Waters XBridge BEH HILIC HPLC Columns

The XBridge BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

The maximum operating pressure for the XBridge BEH HILIC XP Column is up to 18000 psi (1240 Bar). It is important to note that pressures greater than the range of 18000 psi should be avoided as they can result in reduced column lifetime and decreased column performance.

Waters: XBridge BEH HILIC HPLC Columns

Specifications

• Chemistry: HILIC
• Separation Mode: Hydrophilic Interaction (HILIC)
• Particle Substrate: Hybrid
• pH Range Min: 1 pH
• pH Range Max: 9 pH
• Maximum Pressure: 18000 psi (1240 Bar)
• Endcapped: No
• Silanol Activity: Low
• Particle Shape: Spherical
• System: UHPLC, UPLC
• Particle Technology: BEH
• USP Classification: L3

The Waters XBridge BEH HILIC HPLC Column is also available in these types:

Waters XBridge BEH HILIC Column with eConnect Column Tag

• This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.

Waters: XBridge BEH HILIC Column with eConnect Column Tag

XBridge Columns

Particle stability and performance for methods development

Withstanding extreme operating conditions like high pH and high temperatures is essential for methods development. XBridge HPLC and UHPLC Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology and are built to withstand high pH, high temperatures, and other extreme operating conditions like volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis.

XBridge HPLC and UHPLC Columns are available in a broad range of sorbent particles and sizes: XBridge eXtended Perfomance (XP) 2.5 µm Columns for rapid analytical UHPLC separations; 3.5 and 5 µm HPLC columns for reliable analytical method development; and 5 and 10 µm optimum bed density (OBD) preparative columns for purification chromatography.

Waters: XBridge Columns