Thermo Scientific Acclaim HILIC-10 HPLC Columns

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.

Rugged and Reproducible Separations of Highly Polar Compounds:

• Unique selectivity, complementary to reversed-phase columns
• Enhanced sensitivity for MS detection
• Hydrolytically stable
• Rugged column packing
• Broad application range, including separation of hydrophilic drugs and drug metabolites

Acclaim HILIC-10 LC columns are available in 3μm particle size and a variety of column formats: 4.6×150mm, for high-throughput routine analysis; 3.0×150mm, for high-throughput analysis with reduced solvent consumption; and 2.1×150mm, for LC-MS analysis.

Specifications

• Column type: HILIC
• Max. Pressure: 6000 psi (415 bar)
• Packing Material: Silica
• Surface Area: 300 m²/g
• Temperature: 70°C
• USP Type: N/A
• Carbon load: 8%
• Phase: HILIC
• Pore Size: 120 Å
• pH: 2 to 8

About the Acclaim Family of HPLC Columns

Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.

• Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs
• Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations

Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns