Thermo Scientific Acclaim Mixed Mode HILIC-1 HPLC Columns

Get all the advantages of reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.

Separates Both Polar and Nonpolar Molecules with Selectivity Complementary to RP Columns

• Operates in both RP and HILIC modes
• Retains highly polar molecules
• Covers broader application range than conventional diol-based columns, including nonionic ethoxylated surfactants, drug metabolites, lipids, and polyethylene glycols (PEGs)
• Stable between pH 2.5 and 7.5
• Available in 3 and 5μm particle sizes with an average pore diameter of 120Å

Specifications

• Column type: Mixed mode
• For use with: HILIC
• Max. Pressure: 5800 psi (400 bar)
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Stationary phase: Hydrophobic Alkyl Chain with a Diol Group
• USP Type: N/A
• Surface Area: 300 m²/g
• Temperature: 50°C
• Phase: Reversed Phase, HILIC
• Pore Size: 120 Å
• pH: 2.5 to 7.5

About the Acclaim Family of HPLC Columns

Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.

• Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs
• Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations

Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns