rHGH fragment or AOD-9604 (HPLC) analysis

The rHGH Fragment or AOD-9604 (HPLC) Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of rHGH fragment 176-191 and AOD-9604 in lyophilised vials and raw active pharmaceutical ingredient (API) powders.

The analysis can detect and quantify both compounds. For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.

About rHGH Fragment and AOD-9604

rHGH fragment 176-191 is a synthetic peptide corresponding to the C-terminal region of human growth hormone. AOD-9604 is a closely related modified analogue that differs at the N-terminal position, where tyrosine is present instead of phenylalanine.

Both peptides have been studied primarily in relation to lipid metabolism and their effects on adipose tissue. AOD-9604 was developed from research investigating the metabolic activity of the C-terminal region of human growth hormone.

Key Features

• Targeted identification and quantification of rHGH fragment 176-191 and AOD-9604
• Detection and measurement of both compounds
• Suitable for lyophilised vials and raw API powders
• Analysis by HPLC
• Results for lyophilised samples reported in mg per vial and peptide purity
• Alternative result format for raw API powders includes purity in % (m/m) and peptide purity
• Several solvent options available for samples with solubility issues
• Minimum sample amount of one whole unopened vial with lyophilisate
• Minimum sample amount of 30 mg for raw API powders
• Average turnaround time of 7 business days

Analysis Parameters

• Target compounds: rHGH fragment – Somatropin 176-191, AOD-9604
• CAS number – AOD-9604: 221231-10-3
• Molecular weight – AOD-9604: 1815
• Sequence – AOD-9604: H-Tyr-Leu-Arg-Ile-Val-Gln-Cys(1)-Arg-Ser-Val-Glu-Gly-Ser-Cys(1)-Gly-Phe-OH
• CAS number – rHGH fragment: 66004-57-7
• Molecular weight – rHGH fragment: 1799
• Sequence – rHGH fragment: H-Phe-Leu-Arg-Ile-Val-Gln-Cys(1)-Arg-Ser-Val-Glu-Gly-Ser-Cys(1)-Gly-Phe-OH
• Testing method: HPLC
• Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial
• Result format for lyophilised samples: mg per vial and peptide purity
• Result format for raw API powders: Purity in % (m/m) and peptide purity
• Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate
• Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture
• Average turnaround time: 7 business days

Important Notice

• rHGH fragment and AOD-9604 samples may have solubility issues during sample preparation.
• Ultrapure water is used as the default solvent. If a stronger solvent is required, 5% acetic acid or DMSO can be selected as an additional service.
• Both DMSO and 5% acetic acid should dissolve AOD samples completely, although the choice of solvent may affect the analytical results. Samples prepared with water have a higher probability of solubility issues.
• This analysis is specifically intended for rHGH fragment 176-191 and AOD-9604. It does not automatically screen the sample for other growth hormone fragments, related peptides, degradation products, contaminants, or undeclared substances.
• For lyophilised samples, one whole unopened vial containing lyophilisate is required.
• Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.

Optional Additional Analyses

The following optional laboratory services are available:

• TFA Analysis
• Complete Raw Analytical Data
• Solvent: Acetic Acid
• Solvent: DMSO
• Fentanyl Screening
• Alternative Result Format: Purity in % (m/m)
• Batch Variance Testing
• pH Measurement
• Endotoxin Analysis
• CHNS Mass Report
• LCMS Screening for Peptide Contamination
• Sterility Testing (TAMC + TYMC)
• Heavy Metals Analysis (As, Cd, Pb, Hg)
• Additional Laboratory Report