<h5><strong>Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_326238593f.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_326238593f.jpeg 245w," sizes="100vw" width="1024" height="512"></p><h3><strong>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</strong></h3><p>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.</p><p>So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.</p><div><div><p>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.</p><p>At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (<strong>Figure 1</strong>).</p><p>Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493"></p><p>While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.</p><p>And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.</p><h3><strong>Key takeaways</strong></h3><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h3><strong>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</strong></h3><p>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.</p><p>Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.</p><p>Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.</p><h3><strong>Online SPE analysis provides key benefits like:</strong></h3><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul><p>Let’s explore what this technique looks like in practice.</p><h3><strong>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</strong></h3><p>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (<strong>Figure 2</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"></p><h3><strong>The process for analyzing a hops standard solution is straightforward:</strong></h3><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"><strong> on the SPE column</strong></a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"><strong> analytical column </strong></a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (<strong>Figure 3</strong>).</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"></p><h3><strong>Online SPE for analyzing α- and β-bitter acids in real hops samples</strong></h3><p>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.</p><p>To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.</p><p>The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (<strong>Figure 4</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"></p><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h3><strong>From carefully procured samples to tasty sips</strong></h3><p>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.</p><ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul><p>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf"><strong>check out our application note today</strong></a>.</p><h3><strong>FAQs</strong></h3><div><div><h5><strong>What are the key compounds in hops analyzed by HPLC?</strong></h5><p>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</p></div><div><h5><strong>What column types are recommended for hop acid separations?</strong></h5><p>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</p></div><div><h5><strong>What’s the best workflow for hops analysis?</strong></h5><p>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #Automation</p></blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

Webinars LabRulezLCMS Week 27/2026

<p><strong>The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;</strong></p><p>There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607"></p><p>A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;</p><h4><strong>Evaluation of measurement procedure performance&nbsp;</strong></h4><p>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;</p><ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"></p><h4><strong>Characterisation of candidate reference materials&nbsp;</strong></h4><p>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;</p><ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4><strong>Other ILCs&nbsp;</strong></h4><p>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).</p>

Interlaboratory comparisons other than proficiency testing

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Waters Oasis SPE products

Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs.

<h4><strong>Faster, cleaner, and simpler SPE starts here</strong></h4><p>Analytical chemists worldwide use SPE to remove interfering matrix compounds and enrich the analyte of interest to ensure the generation of high-quality data.</p><p>Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.</p><p>Oasis SPE products deliver unmatched purity, consistency, and quality for sample analysis. Along with seven different SPE chemistries, Oasis SPE products use universal to customized protocols to handle your unique sample.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_2_967d461f2e/Waters-Oasis-SPE-kolonky-2.jpg" alt="Waters: Oasis SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_2_967d461f2e/thumbnail_Waters-Oasis-SPE-kolonky-2.jpg 245w," sizes="100vw" width="950" height="416"></p><h4><strong>Overview</strong></h4><ul><li>Fully water-wettable sorbents increase reproducibility and enable elimination of condition and equilibration steps</li><li>Co-polymer pH stable from 0–14 with no secondary silanol activity</li><li>Optimal sensitivity for small molecule and therapeutic peptide analysis</li><li>Reduced matrix effects for LC-MS/MS analyses</li><li>Unsurpassed selectivity</li><li>Consistent retention and capacity, yielding the highest SPE recovery</li><li>Reduces variability in analytical results and increases robustness of analysis</li><li><strong>Recommended Use: </strong>For the fastest, cleanest, and simplest approach to SPE-LC-MS/MS method development.</li></ul><h4><strong>Solid-Phase Extraction Chemistries</strong></h4><h5><strong>Oasis PRiME HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent for acids, bases, and neutrals</li><li>Cleaner samples in fewer steps with simple three-and two-step protocols, no SPE expertise required</li><li>Removes 95% of common matrix interferences, such as proteins, salts, phospholipids, and pigments</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Ideal for reversed-phase SPE clean-up of samples in routine analysis.</li></ul><h5><strong>Oasis PRiME MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent to selectively retain, concentrate and elute basic compounds</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Cleaner samples in fewer steps with simple and fast 3 and 4-step protocols</li><li>Removes 99% of phospholipids from complex sample matrices</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Highly reproducible target analyte recoveries</li><li>Ideal for basic compound specificity while removing matrix interferences in a simple clean-up.</li></ul><h5><strong>Oasis HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent</li><li>Provides high capacity for acids, bases, and neutrals</li><li>Ideal for ultra-high capacity of very polar compounds.</li></ul><h5><strong>Oasis MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent for bases</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of basic analytes.</li></ul><h5><strong>Oasis MAX</strong></h5><ul><li>Mixed-mode Anion eXchange for acids</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of acidic analytes.</li></ul><h5><strong>Oasis WAX</strong></h5><ul><li>Weak Anion eXchange mixed-mode sorbent for strong acidic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of strong acidic compounds.</li></ul><h5><strong>Oasis WCX</strong></h5><ul><li>Weak Cation eXchange mixed-mode sorbent for strong basic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of all basic analytes, especially strong bases.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png" alt="Waters: Oasis Sorbents" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/thumbnail_Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png 211w," sizes="100vw" width="1511" height="1119"></p><h4><strong>Oasis Formats</strong></h4><h5><strong>Oasis μElution Plate</strong></h5><ul><li>Patented μElution plate design</li><li>No evaporation and reconstitution necessary due to elution volumes as low as 25 µL</li><li>Ideal for SPE clean-up and analyte enrichment of small sample volumes</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg" alt="Waters: Oasis µElution Plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg 245w," sizes="100vw" width="502" height="260"></p><h5><strong>Oasis 96-Well Sample Extraction Plates</strong></h5><ul><li>Innovative, award-winning two-stage well design</li><li>High throughput and high recovery</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg" alt="Waters: Oasis 96-Well Sample Extraction Plates" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg 245w," sizes="100vw" width="525" height="329"></p><h5><strong>Oasis Syringe-Barrel Cartridges</strong></h5><ul><li>Ultra-clean syringe barrel and frits</li><li>Available in cartridges ranging from 1 cc to 60 cc</li><li>Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes</li><li>Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg" alt="Waters Corporation: Oasis Syringe-Barrel Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg 245w," sizes="100vw" width="503" height="311"></p><h5><strong>Oasis Glass Cartridges</strong></h5><ul><li>Ultra-clean glass syringe with Teflon® frit</li><li>For trace level detection and analysis at part-per-trillion (PPT) levels</li><li>Available in 5 cc (200 mg) configuration</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg" alt="Waters: Oasis Glass Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg 188w," sizes="100vw" width="409" height="339"></p><h5><strong>Oasis On-Line Columns and Cartridges</strong></h5><ul><li>For rugged, reproducible, and ultra-fast on-line analysis</li><li>Wide choice of configurations, particle sizes, and sorbent chemistries</li><li>Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX</li><li>High recovery and reproducible results for a wide range of compounds</li><li>Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg" alt="Waters: Oasis On-Line Columns and Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg 168w," sizes="100vw" width="415" height="386"></p><h4><strong>Sample preparation, improved</strong></h4><p>Improving various aspects of chromatography and mass spectrometry analyses often starts with sample preparation. &nbsp;Solid-phase extraction (SPE) or "liquid-solid phase extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing matrix components in a sample. &nbsp;</p><p>SPE is a proven sample preparation tool for simple sample matrix removal, including common interferences like proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including matrix effect reduction and an increase in sample concentration and analyte specificity. Matrix removal also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC and reducing signal suppression by providing clean samples for the MS.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg" alt="Waters: Oasis SPE - LC-MS-MS chromatogram of 1 pgmL of alprazolam from rat plasma" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg 169w," sizes="100vw" width="1024" height="946"></p><h4><strong>Get the cleanest extracts with the Oasis 2x4 method</strong></h4><p>Having a variety of tools for method development allows separation scientists to optimize protocols for maximum sensitivity and selectivity. The Oasis 2x4 method for SPE sorbent and protocol selection for acids, bases, and neutrals produces the cleanest extracts – all by eliminating matrix interferences and sample variability. Get the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis with our two protocols and four sorbents. By following the simple steps outlined in the Oasis 2x4 method, you can achieve high recoveries and the cleanest extracts.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg" alt="Waters: Oasis SPE - Oasis 2 x 4 method development protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/thumbnail_Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg 242w," sizes="100vw" width="1519" height="979"></p><h4><strong>Isolate your compounds with the Oasis HLB method</strong></h4><p>Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg" alt="Waters: Oasis SPE - Generic Method Protocol with Oasis HLB SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg 139w," sizes="100vw" width="1024" height="1153"></p><h4><strong>Multiple contaminant analysis with the 2-step pass-through protocol</strong></h4><p>If you are doing multiple residue screening of veterinary drugs, pesticides and mycotoxins in various food matrices, you may be facing a high concentration of organic solvents. With our simple 2-step protocol (pass-through SPE), you can remove matrix interferences quickly if your samples contain a high concentration of organic solvent(s) and concentration and/or salt removal is not required. It can be used as the clean-up method following QuEChERS extraction, saving you significant time compared to the traditional dispersive SPE method - all while achieving superior clean-up.</p><p><strong>To do pass-through SPE, Waters offers 2 convenient and flexible formats:</strong></p><ul><li><strong>Traditional-style cartridges</strong> compatible with vacuum and positive pressure manifolds&nbsp;</li><li><strong>"Plus" style cartridges</strong> that can be used without manifolds when connected to a displacement syringe</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_3_b4da6242de/Waters-Oasis-SPE-kolonky-3.jpg" alt="Waters: Oasis Plus Light and Plus Short Cartridges for 2-step pass through protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_3_b4da6242de/thumbnail_Waters-Oasis-SPE-kolonky-3.jpg 152w," sizes="100vw" width="1308" height="1342"></p>

Products from category SPE sample preparation from Waters - page 1

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