Waters Oasis SPE products

Faster, cleaner, and simpler SPE starts here

Analytical chemists worldwide use SPE to remove interfering matrix compounds and enrich the analyte of interest to ensure the generation of high-quality data.

Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.

Oasis SPE products deliver unmatched purity, consistency, and quality for sample analysis. Along with seven different SPE chemistries, Oasis SPE products use universal to customized protocols to handle your unique sample.

Waters: Oasis SPE

Overview

• Fully water-wettable sorbents increase reproducibility and enable elimination of condition and equilibration steps
• Co-polymer pH stable from 0–14 with no secondary silanol activity
• Optimal sensitivity for small molecule and therapeutic peptide analysis
• Reduced matrix effects for LC-MS/MS analyses
• Unsurpassed selectivity
• Consistent retention and capacity, yielding the highest SPE recovery
• Reduces variability in analytical results and increases robustness of analysis
• Recommended Use: For the fastest, cleanest, and simplest approach to SPE-LC-MS/MS method development.

Solid-Phase Extraction Chemistries

Oasis PRiME HLB

• Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent for acids, bases, and neutrals
• Cleaner samples in fewer steps with simple three-and two-step protocols, no SPE expertise required
• Removes 95% of common matrix interferences, such as proteins, salts, phospholipids, and pigments
• No sorbent conditioning or equilibration steps required
• Faster, more predictable analysis time
• Ideal for reversed-phase SPE clean-up of samples in routine analysis.

Oasis PRiME MCX

• Mixed-mode Cation eXchange sorbent to selectively retain, concentrate and elute basic compounds
• Reversed-phase and ion-exchange functionality for orthogonal sample preparation
• Cleaner samples in fewer steps with simple and fast 3 and 4-step protocols
• Removes 99% of phospholipids from complex sample matrices
• No sorbent conditioning or equilibration steps required
• Faster, more predictable analysis time
• Highly reproducible target analyte recoveries
• Ideal for basic compound specificity while removing matrix interferences in a simple clean-up.

Oasis HLB

• Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent
• Provides high capacity for acids, bases, and neutrals
• Ideal for ultra-high capacity of very polar compounds.

Oasis MCX

• Mixed-mode Cation eXchange sorbent for bases
• Increased analyte specificity and sensitivity
• Reversed-phase and ion-exchange functionality for orthogonal sample preparation
• Ideal for selective retention of basic analytes.

Oasis MAX

• Mixed-mode Anion eXchange for acids
• Increased analyte specificity and sensitivity
• Reversed-phase and ion-exchange functionality for orthogonal sample preparation
• Ideal for selective retention of acidic analytes.

Oasis WAX

• Weak Anion eXchange mixed-mode sorbent for strong acidic compounds
• Increased analyte specificity and sensitivity
• Reversed-phase and ion-exchange functionality for orthogonal sample preparation
• Ideal for improved retention of strong acidic compounds.

Oasis WCX

• Weak Cation eXchange mixed-mode sorbent for strong basic compounds
• Increased analyte specificity and sensitivity
• Reversed-phase and ion-exchange functionality for orthogonal sample preparation
• Ideal for improved retention of all basic analytes, especially strong bases.

Waters: Oasis Sorbents

Oasis Formats

Oasis μElution Plate

• Patented μElution plate design
• No evaporation and reconstitution necessary due to elution volumes as low as 25 µL
• Ideal for SPE clean-up and analyte enrichment of small sample volumes
• Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)

Waters: Oasis µElution Plate

Oasis 96-Well Sample Extraction Plates

• Innovative, award-winning two-stage well design
• High throughput and high recovery
• Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)

Waters: Oasis 96-Well Sample Extraction Plates

Oasis Syringe-Barrel Cartridges

• Ultra-clean syringe barrel and frits
• Available in cartridges ranging from 1 cc to 60 cc
• Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes
• Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes

Waters Corporation: Oasis Syringe-Barrel Cartridges

Oasis Glass Cartridges

• Ultra-clean glass syringe with Teflon® frit
• For trace level detection and analysis at part-per-trillion (PPT) levels
• Available in 5 cc (200 mg) configuration

Waters: Oasis Glass Cartridges

Oasis On-Line Columns and Cartridges

• For rugged, reproducible, and ultra-fast on-line analysis
• Wide choice of configurations, particle sizes, and sorbent chemistries
• Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX
• High recovery and reproducible results for a wide range of compounds
• Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available

Waters: Oasis On-Line Columns and Cartridges

Sample preparation, improved

Improving various aspects of chromatography and mass spectrometry analyses often starts with sample preparation.  Solid-phase extraction (SPE) or "liquid-solid phase extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing matrix components in a sample.  

SPE is a proven sample preparation tool for simple sample matrix removal, including common interferences like proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including matrix effect reduction and an increase in sample concentration and analyte specificity. Matrix removal also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC and reducing signal suppression by providing clean samples for the MS.

Waters: Oasis SPE - LC-MS-MS chromatogram of 1 pgmL of alprazolam from rat plasma

Get the cleanest extracts with the Oasis 2x4 method

Having a variety of tools for method development allows separation scientists to optimize protocols for maximum sensitivity and selectivity. The Oasis 2x4 method for SPE sorbent and protocol selection for acids, bases, and neutrals produces the cleanest extracts – all by eliminating matrix interferences and sample variability. Get the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis with our two protocols and four sorbents. By following the simple steps outlined in the Oasis 2x4 method, you can achieve high recoveries and the cleanest extracts.

Waters: Oasis SPE - Oasis 2 x 4 method development protocol.

Isolate your compounds with the Oasis HLB method

Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.

Waters: Oasis SPE - Generic Method Protocol with Oasis HLB SPE

Multiple contaminant analysis with the 2-step pass-through protocol

If you are doing multiple residue screening of veterinary drugs, pesticides and mycotoxins in various food matrices, you may be facing a high concentration of organic solvents. With our simple 2-step protocol (pass-through SPE), you can remove matrix interferences quickly if your samples contain a high concentration of organic solvent(s) and concentration and/or salt removal is not required. It can be used as the clean-up method following QuEChERS extraction, saving you significant time compared to the traditional dispersive SPE method - all while achieving superior clean-up.

To do pass-through SPE, Waters offers 2 convenient and flexible formats:

• Traditional-style cartridges compatible with vacuum and positive pressure manifolds 
• "Plus" style cartridges that can be used without manifolds when connected to a displacement syringe

Waters: Oasis Plus Light and Plus Short Cartridges for 2-step pass through protocol.