Quantifying steroids in biological matrices has always presented analytical challenges. Complex structures, isobaric interferences, and matrix effects often limit accuracy and throughput — demanding a new approach beyond incremental improvements.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg 245w," sizes="100vw" width="1024" height="512">The<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149"> Thermo Scientific Stellar MS</a> redefines how analysts approach steroid quantification, enabling rapid method optimization through advanced scanning flexibility, complementary fragmentation mechanisms, and enhanced selectivity.Rather than focusing on a single metric, this application note demonstrates how researchers can quickly develop more effective methods by leveraging the full capability of the Stellar MS to characterize compounds comprehensively in a single acquisition.<h3>A new paradigm for analytical quantitation</h3>Traditional LC-MS approaches often rely on incremental improvements in sensitivity or speed. Stellar MS instead provides a new analytical framework, designed to optimize every parameter of quantitative method creation.Key attributes include:<ol><li>PRM vs. SRM/MRM — improving instrument efficiency Parallel Reaction Monitoring (PRM) on Stellar MS provides comprehensive ion information for each target steroid, improving quantitation accuracy and efficiency over traditional MRM methods.</li><li>Complementary dissociation mechanisms — HCD and CID Stellar’s unique ability to combine beam-type (HCD) and resonant (CID) dissociation ensures full coverage of all 17 targeted steroids. This dual-mode approach effectively overcomes neutral water loss product ions — one of the key challenges in steroid analysis.</li><li>Next-level selectivity — MS² to MS³ transition The platform’s ability to perform MS³ experiments enhances selectivity and confidence, particularly in complex biological matrices, where interferences are common.</li><li>Product ion accumulation vs. dwell time — improved ion ratios and sensitivity By accumulating product ions rather than relying on fixed dwell times, Stellar MS achieves superior ion ratio precision and reproducibility — even at low spiking levels — without sacrificing throughput.</li></ol>&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer: Figure 1. Representative EIC of 17 steroids in human serum using the Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg 245w," sizes="100vw" width="800" height="412"><h3>From characterization to confident method creation</h3>The power of Stellar MS lies in its comprehensive acquisition strategy. In this application note, a single injection per spiking level generated 125 distinct scan events, combining:<ul><li>HCD and CID MS² experiments</li><li>Targeted MS³ scans</li><li>Positive/negative polarity switching</li></ul>This workflow enables analysts to rapidly evaluate:<ol><li>Which dissociation mechanism produces the most informative spectra</li><li>Whether MS² or MS³ is best suited for each compound</li><li>The optimal product ions for confident post-acquisition quantitation</li></ol>By providing this complete dataset in a single run, Stellar MS dramatically accelerates method optimization and validation, supporting more efficient and reliable quantitation strategies.<h3>Driving method development efficiency</h3>The real strength of the Stellar MS isn’t just its speed or sensitivity — it’s the ability to experiment, compare, and decide in one workflow. Through simultaneous HCD/CID fragmentation, MS² and MS³ switching, and polarity control, users can determine the optimal analytical conditions across complex steroid panels quickly and confidently.This approach helps labs:<ul><li>Reduce development time</li><li>Maximize data quality</li><li>Improve long-term robustness and reproducibility</li></ul><h3>Conclusion</h3>The Stellar MS platform empowers analytical scientists to move beyond incremental performance metrics toward methodological innovation.By integrating comprehensive characterization and advanced scanning control, it enables faster, more confident decisions in quantitative steroid analysis — setting a new benchmark for flexibility and precision in LC-MS.Ask the Experts: On-Demand WebinarLearn more about how Stellar MS enhances steroid profiling and quantitative workflows. Watch the on-demand webinar where Thermo Fisher scientists discuss:<ul><li>Advanced method development strategies</li><li>Insights from the steroid panel dataset</li><li>Practical examples of leveraging Stellar’s flexibility for confident quantitation</li></ul><blockquote>Watch now: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5117">Unlocking the Power of Quantitative Analysis with Stellar MS</a>Read the full application note: <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/32415">Selective and sensitive measurement of 17 steroids in human serum using a Stellar mass spectrometer</a></blockquote><ul><li>Learn more about <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/stellar-mass-spectrometer/stellar-mass-spectrometer-applications.html">Stellar Mass Spectrometer applications</a></li><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#MassSpectrometry #ClinicalResearch #LCMS #StellarMS</li></ul><blockquote><h5>Dane Walker</h5>Dane Walker is Regional Marketing Manager at Thermo Fisher Scientific, focused on LC-MS systems across EMEA. With a PhD in Surface Science and a background in scientific instrumentation, he blends technical expertise with strategic marketing to drive data-led campaigns. Passionate about communicating science and enabling innovation, Dane enjoys powerboating, cycling, stargazing, and talking endlessly about mass spectrometry.</blockquote>

Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer

<h4>The problem&nbsp;</h4>We live in a “global village” but we are all different and we use many different languages to communicate.&nbsp;Even when people speak the same language, the same words may be used with different meanings. See, for example, some different uses of the word “standard” in English – a normative document, a solution of known concentration or even a type of flag.&nbsp;Even in the same area of activity misunderstandings do occur which may result in a waste of time and money, or even worse consequences.&nbsp;When talking about laboratory tests, lack of a full understanding of key terms and inappropriate translations leaves room for different interpretations of stated requirements. For example, there have historically been a number of different but related uses of the term ‘detection limit’. This may lead to inadequate fulfilment of those requirements, thus becoming an obstacle to the production of analytical data that is fit for its intended use. A shared interpretation of concepts related to quality in measurement between laboratories and accreditation bodies is also crucial for a fair and harmonised approach to laboratory assessment across the world.&nbsp;<h4>A common language&nbsp;</h4>We need a common language; clear and unambiguous. We need a common vocabulary to provide consistent definitions of concepts with their associated terms. To this end, several organisations participate in a joint effort to develop and maintain an international vocabulary of metrology (VIM) [1], which aims to achieve a unique understanding of concepts related to measurements across all sectors. The VIM is a normative reference in the standards ISO/IEC 17025, ISO 15189 and ISO/IEC 17043, and is therefore a key document for all organisations seeking accreditation.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_Standards_8d947dbd5f.jpg" alt="Eurachem: You talk, we understand – The way out of the tower of Babel_A common language" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_Standards_8d947dbd5f.jpg 245w," sizes="100vw" width="1126" height="499"><h4>Is this enough?&nbsp;</h4>For laboratory staff some problems still stand and need to be addressed:&nbsp;<ul><li>Many people are confused about both concepts and terms.&nbsp;</li><li>It may be difficult to understand the “formal” VIM definitions – they are short statements intended to apply to many different measurement sectors.</li><li>Translations into local languages may add to the confusion, e.g. if different terms are used in different sectors for the same VIM concept and the translator overlooks this issue.</li><li>Some substantial changes to terminology have been made in an attempt to accommodate chemical and biological measurements. Some “old” concepts still stand but have new names, e.g. “within laboratory reproducibility” vs. “intermediate precision”.&nbsp;</li></ul>To address these issues Eurachem has produced a guide [2] which provides an accessible reference containing simple and in context explanations of terms most relevant to analysts, with additional examples relating those concepts to chemical and biological measurements.&nbsp;<h4>Do you understand the meaning of these terms?&nbsp;</h4>Are you interested in a quantity or a quality? Depending on the answer, will you use a measurement procedure or an examination? Is method verification the same or less demanding than method validation? Do you need a calibration or just performance verification? Do you have a measurement standard in your laboratory? Is metrological traceability something you worry about? These are examples of the terms supporting the reliability of any measurement result that need to be clearly understood.&nbsp;<h5>Example – Accuracy, trueness, precision: are they synonyms?&nbsp;</h5><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_accuracy_231e1e005e.jpg" alt="Eurachem: You talk, we understand – The way out of the tower of Babel_Example – Accuracy, trueness, precision" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_You_talk_we_understand_The_way_out_of_the_tower_of_Babel_accuracy_231e1e005e.jpg 146w," sizes="100vw" width="754" height="807"><ul><li>Closeness between measurement results (relating to the concept of precision)</li><li>Closeness of the average of the measurement results to a reference value (relating to the concept of trueness)&nbsp;</li><li>Closeness of an individual measurement result to the true value (relating to the concept of accuracy)&nbsp;</li></ul>The different situations in the figure show the effect of:&nbsp;<ol style="list-style-type:lower-latin;"><li>poor precision and trueness (imprecise and biased)&nbsp;</li><li>good precision, poor trueness (precise but biased)&nbsp;</li><li>poor precision, good trueness (imprecise but unbiased)&nbsp;</li><li>good precision and trueness (precise and unbiased)&nbsp;</li></ol>Improving both precision and trueness is expected to improve accuracy.&nbsp;<h4>Is all this relevant to you?&nbsp;</h4>Do you work in a laboratory, either as an analyst, quality manager or director? Are you involved in other activities related to analytical work, such as providing proficiency tests, producing reference materials or accrediting any such activity? Do you teach or study subjects related to measurements? Do you use measurement results or prescribe how to use them? If the answer is yes to any of these questions, the following reference documents are also for you.

You talk, we understand – The way out of the tower of Babel

Visit the LabRulez booth at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871">analytica 2026</a> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"><h3>Would you like to learn how the LabRulez portals work?</h3>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/">LabRulezLCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/">LabRulezGCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/">LabRulezICPMS</a><ul><li>A unique online solution for your marketing</li><li>A targeted audience from the analytical chemistry community</li><li>Modern technologies and a unique concept</li><li>Design optimized for most smart devices – PC, mobile, and tablet</li><li>Advanced statistical tools fully available to users</li></ul><h3>Services Offered</h3><h4>News + Company Updates</h4><ul><li>Publish news related to your instrumentation, applications, services, events, job openings, and more</li><li>News is shared both on our portals and across our social media channels</li></ul><h4>Library – All Documents in One Place</h4><ul><li>Applications, posters, presentations, technical articles, brochures, specifications</li><li>Fast and advanced search capabilities</li><li>Over 32,000 documents currently available in our database</li></ul><h4>Webinars</h4><ul><li>Upcoming and on-demand webinars from the field of analytical chemistry</li><li>More than 5,500 webinars covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4>Careers</h4><ul><li>Targeted job listings in analytical chemistry</li><li>Clear filtering by specialization or location</li><li>Increased visibility for job postings</li></ul><h4>Companies</h4><ul><li>Company profile with key information and contacts</li><li>Integration of all your activities on our portal: News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li>Connection to your Products listed on LabRulez portals</li></ul><h4>Products and Services</h4><ul><li>Comprehensive overview of instrumental techniques and services</li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4>Marketplace</h4><ul><li>Buying and selling pre-owned analytical equipment</li><li>We purchase analytical instrumentation of all brands and ages</li><li>Sell your equipment through LabRulez</li><li>Request and purchase complete systems, modules, or spare parts with warranty and service</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"><h3>What Will Be on Display at Our Booth?</h3>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar">LabRulezLCMS Marketplace</a></h4>Spare parts for LC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378">Vacuum turbo pumps for LC/MS</a></li></ul>Spare parts for HPLC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397">Degassers for HPLC/UHPLC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve">Automatic valves for HPLC</a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377">HPLC lamps</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48">HPLC columns</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar">LabRulezGCMS Marketplace</a></h4>Spare parts for GC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo">Turbo pumps for GC/MS</a></li></ul>Spare parts for GC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC">EPC modules</a></li></ul>Sample injection<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432">Autosampler 7693A for GC</a></li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7">GC columns </a>and selected accessories</li></ul>

LabRulez at analytica 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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Watrex BioWorks WorkBeads resins and GoBio Mini columns

Watrex Praha is the official distributor of Bio-Works, a leading global manufacturer of chromatographic resins designed for the purification of biopharmaceuticals.

<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex Praha</a> is the official distributor of Bio-Works, a leading global manufacturer of chromatographic resins designed for the purification of biopharmaceuticals. This partnership brings high-quality products to the Czech market, widely used in biotechnology and the pharmaceutical industry.The main product of Bio-Works is <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads </a>resins, manufactured using a patented agarosebased method. These resins are characterized by excellent mechanical stability, uniform bead size distribution, and high separation efficiency. They are available both as loose resins and in prepacked columns, making them easy to use in various applications.For efficient small-scale purification and process development screening, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio Mini</a> columns are the ideal solution. These prepacked columns are available in 1 ml and 5 ml sizes, facilitating quick and effective biomolecule separations.Bio-Works products are used in bioprocess solutions focused on the purification and analysis of biomolecules, including monoclonal antibodies, proteins, peptides, oligonucleotides, viruses, and vaccines. These technologies offer top-tier quality and precision, ensuring optimal capacity and product purity.<h3>Types of sorbents:</h3><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/affinity-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Affinity chromatography resins</a></h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/affinity-chromatography/affinity-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads™ affimAb</a> is an optimized alkali-stable protein A resin designed for purification of monoclonal- and polyclonal antibodies in laboratory to process scale. This resin has a superior base matrix combined with a patented, alkali-stable protein A ligand, which results in high dynamic binding capacity at short residence times. This resin binds over 40 mg IgG/mL resin also at as short residence time as 2.5 min. Available also in prepacked 1 mL and 5 mL <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/affinity-chromatography/affinity-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio™ Mini </a>columns.<h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/ion-exchange-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Ion exchange chromatography (IEX) resins</a></h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/ion-exchange-chromatography/iex-sorbents/">WorkBeads™ resins for ion exchange chromatography</a> are designed for research and industrial scale purification of proteins, peptides and oligonucleotides by utilizing the difference in their surface charge. These resins demonstrate high-resolution separation while giving low backpressure to facilitate both capture and polishing purification applications in standard bioprocess columns.<ul><li>WorkBeads 100S is a strong cation exchanger and will bind positively charged substances.</li><li>WorkBeads 100Q is a strong anion exchanger and will bind negatively charged substances.</li><li>WorkBeads 40S is a strong cation exchanger with sulfonate ligands and will bind positively charged substances.&nbsp;</li><li>WorkBeads 40Q is a strong anion exchanger with quaternary amine and will bind negatively charged substances.&nbsp;</li><li>WorkBeads 40 DEAE is a weak anion exchanger with tertiary amine ligands (diethylaminoethyl) and is an alternative to WorkBeads 40Q when looking for alternative selectivities.</li></ul>Also available in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/ion-exchange-chromatography/iex-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio™ Mini.</a><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Size exclusion chromatography (SEC) resins</a></h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads™ 40/100 SEC, WorkBeads 40/1000 SEC, WorkBeads 40/10 000 SEC, and</a> <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads Macro SEC resins</a> are used for preparative size exclusion chromatography (SEC) in laboratory and process scale purification of proteins, viruses, and other biomolecules by utilizing the differences in their size. These resins are based on agarose, which is a biopolymer suitable for separation of biomolecules. WorkBeads resins are cross-linked using a proprietary method that results in a very rigid structure. Although the general recommendation for SEC is to use a low flow rate for best purification, the rigidity and tight particle size distribution allow for the purification of viruses and other large substances at a high flow rate for fast processing and high yields.Produced using a proprietary cross-linking method that results in highly porous and physically stable matrices.We have recently added two new Size Exclusion Chromatography (SEC) products that expand the range for more choice and flexibility in separating large particles. WorkBeads 200 SEC, with 180 μm beads, is suitable for viscous samples like serum and whole blood. WorkBeads Macro SEC resins extend the range of several resins with a bead size of 45 μm but different porosities, making it easy to change between them when different fractionation ranges are desired.<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads Dsalt resin</a> is designed to enable quick and easy separations of high- and lowmolecular-weight substances. This resin is pre-swollen and enables efficient desalting and buffer exchange of proteins, large peptides, and nucleic acids.Also available in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio™ Mini</a>.<h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/hic-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Hydrophobic interaction chromatography (HIC) resins</a></h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/hic-chromatography/hic-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads™ 40 Butyl SH</a> is a resin for hydrophobic interaction chromatography (HIC) designed for research and industrial scale purification of proteins, peptides, plasmids, and oligonucleotides by utilizing the difference in their surface hydrophobicity. It’s often used to complement other techniques that separate according to either charge or size.The functional ligand of WorkBeads 40 Butyl SH is n-butyl thioether. Since butyl is a very hydrophobic linear chain, minimal mixed-mode interactions are expected. The resin is optimized to offer reliable binding performance. The property of high-resolution separation, in combination with low backpressure, allows both capture and polishing purification applications in standard bioprocess columns. The resin is also available in several different ready-to-use prepacked column sizes, such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/hic-chromatography/hic-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio™ Mini</a> 1 mL and 5 mL, GoBio Screen 7 x 100 (3.8 mL), GoBio Prep 16 x 100 (20 mL) and 26 x 100 (53 mL) as well as GoBio Prod columns starting at 1 L. 5.&nbsp;<h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/imac-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Immobilized metal-ion affinity chromatography (IMAC) resins&nbsp;</a></h4>There are three different chelating ligands that <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/imac-chromatography/imac-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeads IMAC resins</a> are based on. First are WorkBeads IDA (iminodiacetic acid) and WorkBeads NTA (nitrilotriacetic acid), which can be charged or are pre-charged with four different metals (Ni2+, Co2+, Cu2+, and Zn2+). Different combinations of ligands and metals give unique selectivities. Pre-charged IDA and NTA-based IMAC resins can be stripped, cleaned, and recharged with fresh metal ions.&nbsp;The third resin is WorkBeads NiMAC, a nickel pre-charged resin with very low nickel leakage and tolerant against chelating agents (EDTA) and reducing substances (DTT). This resin is optimal for the purification of, e.g. eukarytic cell feeds, which often containing these substances.For optimization, an initial screening is recommended with the different WorkBeads IMAC resins to identify the optimal combination of ligand and metal ion.Also available in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/imac-chromatography/imac-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio™ Mini.</a><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/activated-resins/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Activated resins</a></h4>Pre-activated resin enables successful, convenient immobilization of ligands without the need for complex syntheses or special equipment. We have developed two different pre-activated resins where the bromohydrin active group reacts with thiol, amino and hydroxyl groups of the substance to be coupled. Two different resin porosities are available to facilitate optimized coupling of ligands of different sizes, or to optimize the prepared affinity resin for target molecules of different sizes.Resin for coupling your own ligands:<ul><li>Made from agarose, well established and well-known in the Biotechnology industry</li><li>Simple coupling procedures at ambient temperature</li><li>Stable at ambient temperature in aqueous solution and at neutral pH</li><li>Suitable for coupling of ligands containing sulphydryl, amino- or hydroxy group</li></ul>Available as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/activated-resins/activated-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">WorkBeadsTM sorbents</a> or in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/activated-resins/activated-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">GoBio™ Mini</a>.For more information or a non-binding quote regarding BioWorks products, please contact us at watrex@watrex.com or visit our website <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">www.watrex.com</a>

Waters Oasis SPE products

Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs.

<h4>Faster, cleaner, and simpler SPE starts here</h4>Analytical chemists worldwide use SPE to remove interfering matrix compounds and enrich the analyte of interest to ensure the generation of high-quality data.Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.Oasis SPE products deliver unmatched purity, consistency, and quality for sample analysis. Along with seven different SPE chemistries, Oasis SPE products use universal to customized protocols to handle your unique sample.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_2_967d461f2e/Waters-Oasis-SPE-kolonky-2.jpg" alt="Waters: Oasis SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_2_967d461f2e/thumbnail_Waters-Oasis-SPE-kolonky-2.jpg 245w," sizes="100vw" width="950" height="416"><h4>Overview</h4><ul><li>Fully water-wettable sorbents increase reproducibility and enable elimination of condition and equilibration steps</li><li>Co-polymer pH stable from 0–14 with no secondary silanol activity</li><li>Optimal sensitivity for small molecule and therapeutic peptide analysis</li><li>Reduced matrix effects for LC-MS/MS analyses</li><li>Unsurpassed selectivity</li><li>Consistent retention and capacity, yielding the highest SPE recovery</li><li>Reduces variability in analytical results and increases robustness of analysis</li><li>Recommended Use: For the fastest, cleanest, and simplest approach to SPE-LC-MS/MS method development.</li></ul><h4>Solid-Phase Extraction Chemistries</h4><h5>Oasis PRiME HLB</h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent for acids, bases, and neutrals</li><li>Cleaner samples in fewer steps with simple three-and two-step protocols, no SPE expertise required</li><li>Removes 95% of common matrix interferences, such as proteins, salts, phospholipids, and pigments</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Ideal for reversed-phase SPE clean-up of samples in routine analysis.</li></ul><h5>Oasis PRiME MCX</h5><ul><li>Mixed-mode Cation eXchange sorbent to selectively retain, concentrate and elute basic compounds</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Cleaner samples in fewer steps with simple and fast 3 and 4-step protocols</li><li>Removes 99% of phospholipids from complex sample matrices</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Highly reproducible target analyte recoveries</li><li>Ideal for basic compound specificity while removing matrix interferences in a simple clean-up.</li></ul><h5>Oasis HLB</h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent</li><li>Provides high capacity for acids, bases, and neutrals</li><li>Ideal for ultra-high capacity of very polar compounds.</li></ul><h5>Oasis MCX</h5><ul><li>Mixed-mode Cation eXchange sorbent for bases</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of basic analytes.</li></ul><h5>Oasis MAX</h5><ul><li>Mixed-mode Anion eXchange for acids</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of acidic analytes.</li></ul><h5>Oasis WAX</h5><ul><li>Weak Anion eXchange mixed-mode sorbent for strong acidic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of strong acidic compounds.</li></ul><h5>Oasis WCX</h5><ul><li>Weak Cation eXchange mixed-mode sorbent for strong basic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of all basic analytes, especially strong bases.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png" alt="Waters: Oasis Sorbents" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/thumbnail_Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png 211w," sizes="100vw" width="1511" height="1119"><h4>Oasis Formats</h4><h5>Oasis μElution Plate</h5><ul><li>Patented μElution plate design</li><li>No evaporation and reconstitution necessary due to elution volumes as low as 25 µL</li><li>Ideal for SPE clean-up and analyte enrichment of small sample volumes</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg" alt="Waters: Oasis µElution Plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg 245w," sizes="100vw" width="502" height="260"><h5>Oasis 96-Well Sample Extraction Plates</h5><ul><li>Innovative, award-winning two-stage well design</li><li>High throughput and high recovery</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg" alt="Waters: Oasis 96-Well Sample Extraction Plates" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg 245w," sizes="100vw" width="525" height="329"><h5>Oasis Syringe-Barrel Cartridges</h5><ul><li>Ultra-clean syringe barrel and frits</li><li>Available in cartridges ranging from 1 cc to 60 cc</li><li>Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes</li><li>Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg" alt="Waters Corporation: Oasis Syringe-Barrel Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg 245w," sizes="100vw" width="503" height="311"><h5>Oasis Glass Cartridges</h5><ul><li>Ultra-clean glass syringe with Teflon® frit</li><li>For trace level detection and analysis at part-per-trillion (PPT) levels</li><li>Available in 5 cc (200 mg) configuration</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg" alt="Waters: Oasis Glass Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg 188w," sizes="100vw" width="409" height="339"><h5>Oasis On-Line Columns and Cartridges</h5><ul><li>For rugged, reproducible, and ultra-fast on-line analysis</li><li>Wide choice of configurations, particle sizes, and sorbent chemistries</li><li>Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX</li><li>High recovery and reproducible results for a wide range of compounds</li><li>Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg" alt="Waters: Oasis On-Line Columns and Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg 168w," sizes="100vw" width="415" height="386"><h4>Sample preparation, improved</h4>Improving various aspects of chromatography and mass spectrometry analyses often starts with sample preparation. &nbsp;Solid-phase extraction (SPE) or "liquid-solid phase extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing matrix components in a sample. &nbsp;SPE is a proven sample preparation tool for simple sample matrix removal, including common interferences like proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including matrix effect reduction and an increase in sample concentration and analyte specificity. Matrix removal also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC and reducing signal suppression by providing clean samples for the MS.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg" alt="Waters: Oasis SPE - LC-MS-MS chromatogram of 1 pgmL of alprazolam from rat plasma" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg 169w," sizes="100vw" width="1024" height="946"><h4>Get the cleanest extracts with the Oasis 2x4 method</h4>Having a variety of tools for method development allows separation scientists to optimize protocols for maximum sensitivity and selectivity. The Oasis 2x4 method for SPE sorbent and protocol selection for acids, bases, and neutrals produces the cleanest extracts – all by eliminating matrix interferences and sample variability. Get the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis with our two protocols and four sorbents. By following the simple steps outlined in the Oasis 2x4 method, you can achieve high recoveries and the cleanest extracts.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg" alt="Waters: Oasis SPE - Oasis 2 x 4 method development protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/thumbnail_Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg 242w," sizes="100vw" width="1519" height="979"><h4>Isolate your compounds with the Oasis HLB method</h4>Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg" alt="Waters: Oasis SPE - Generic Method Protocol with Oasis HLB SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg 139w," sizes="100vw" width="1024" height="1153"><h4>Multiple contaminant analysis with the 2-step pass-through protocol</h4>If you are doing multiple residue screening of veterinary drugs, pesticides and mycotoxins in various food matrices, you may be facing a high concentration of organic solvents. With our simple 2-step protocol (pass-through SPE), you can remove matrix interferences quickly if your samples contain a high concentration of organic solvent(s) and concentration and/or salt removal is not required. It can be used as the clean-up method following QuEChERS extraction, saving you significant time compared to the traditional dispersive SPE method - all while achieving superior clean-up.To do pass-through SPE, Waters offers 2 convenient and flexible formats:<ul><li>Traditional-style cartridges compatible with vacuum and positive pressure manifolds&nbsp;</li><li>"Plus" style cartridges that can be used without manifolds when connected to a displacement syringe</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_3_b4da6242de/Waters-Oasis-SPE-kolonky-3.jpg" alt="Waters: Oasis Plus Light and Plus Short Cartridges for 2-step pass through protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_3_b4da6242de/thumbnail_Waters-Oasis-SPE-kolonky-3.jpg 152w," sizes="100vw" width="1308" height="1342">

Phenomenex Strata X-AW Solid Phase Extraction (SPE)

Strata-X-AW mixed-mode SPE sorbent enables strong retention of acids (pKa ≤5) with 100% organic wash, ensuring maximum removal of interferences for clean, reliable extractions.

Weak Anion Mixed-Mode Solid Phase Extraction (SPE)A weak anion-exchange functionalized polymeric sorbent that allows for complete retention of acidic compounds with a pKa less than 5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg" alt="Phenomenex: Strata-X-AW SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_AW_SPE_cd5908db4d.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of acidic compounds, particularly strong acids</li></ul><h4>Starting Method</h4><h5>For Acids with pKa ≤ 5</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample pH 6-7</li><li>Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li>Wash 2: 1 mL Methanol</li><li>Elute Any Acid: 2x 500 µL 5 % NH4OH in Methanol</li><li>Elute Weak Acids: 2x 500 µL 5 % Formic Acid in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-A Solid Phase Extraction (SPE)

Strata-X-A strong anion-exchange SPE retains weak acids (pKa >2) with 100% organic wash, maximizing interference removal. Available in tubes, plates, and giga formats.

Strong Anion Mixed-Mode Solid Phase Extraction (SPE)A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with a pKa greater than 2, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg" alt="Phenomenex: Strata-X-A SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_A_SPE_e107f0b6bf.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of weakly acidic compounds</li></ul><h4>Starting Method</h4><h5>For Acids with pKa &gt; 2</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample pH 6-7</li><li>Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7</li><li>Wash 2: 1 mL Methanol (collect this fraction to analyze Neutral/Bases)</li><li>Elute Acid: 2x 500 µL 5 % Formic Acid in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-C Solid Phase Extraction (SPE)

Strata-X-C strong cation-exchange SPE retains basic compounds (pKa ≤10.5) with 100% organic wash for maximum cleanup. Offered in tubes, plates, and giga formats.

Strong Cation Mixed-Mode Solid Phase Extraction (SPE)A strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa less than 10.5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_C_SPE_4927d7340d.jpg" alt="Phenomenex: Strata-X-C SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_C_SPE_4927d7340d.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of weakly basic compounds</li></ul><h4>Starting Method</h4><h5>For Bases with pKa ≤ 10.5</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Diluted Acidified Sample</li><li>Wash 1: 1 mL 0.1 N HCI in Water (collect this fraction to analyze Polar Neutrals)</li><li>Wash 2: 1 mL 0.1 N HCI in Methanol (collect this fraction to analyze Neutrals/Acids)</li><li>Elute Bases: 2x 500 µL 5 % NH4OH in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X-CW Solid Phase Extraction (SPE)

Strata-X-CW weak cation-exchange SPE retains strong bases (pKa >8) with 100% organic wash for maximum cleanup. Available in tubes, 96-well plates, and giga formats.

Weak Cation Mixed-Mode Solid Phase Extraction (SPE)A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_CW_SPE_b16a0e0baf.jpg" alt="Phenomenex: Strata-X-CW SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_CW_SPE_b16a0e0baf.jpg 245w," sizes="100vw" width="500" height="268"><ul><li>Recommended Use: Extraction of basic compounds, particularly strong bases</li></ul><h4>Starting Method</h4><h5>For Bases with pKa &gt; 8</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Diluted Sample, pH 6-7</li><li>Wash 1: 1 mL Water, pH 6-7</li><li>Wash 2: 1 mL Methanol (collect this fraction to analyze Neutrals/Acids)</li><li>Elute Any Bases: 2x 500 µL 5 % Formic Acid in Methanol</li><li>Elute Weak Bases: 2x 500 µL 5 % NH4OH in Methanol</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Strata X Solid Phase Extraction (SPE)

Strata-X reversed phase SPE retains neutrals, acids, and bases via π-π, hydrogen bonding, and hydrophobic interactions. Robust cleanup under high organic wash.

Reversed Phase Solid Phase Extraction (SPE)A reversed phase functionalized polymeric sorbent that gives strong retention of neutral, acidic, or basic compounds under aggressive, high organic wash conditions. This sorbent relies on 3 mechanisms of retention: pi-pi bonding, hydrogen bonding (dipole-dipole interactions), and hydrophobic interaction.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_d8f4451e42.jpg" alt="Phenomenex: Strata-X" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_d8f4451e42.jpg 245w," sizes="100vw" width="433" height="247"><ul><li>Recommended Use: Extraction of neutrals and aromatics</li></ul><h4>Starting Method</h4><h5>For Neutral Compounds</h5><ul><li>Column: 1 mL Methanol</li><li>Equilibrate: 1 mL Water</li><li>Load: Diluted Sample</li><li>Wash: 1 mL 5-60 % Methanol</li><li>Elute: 2x 500 µL 2 % Formic Acid in Methanol/Acetonitrile</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_single_tube_435d45d0ad.jpg" alt="Phenomenex: Strata X single tube" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_single_tube_435d45d0ad.jpg 156w," sizes="100vw" width="1080" height="1080">Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.<h4>Available Formats</h4><ul><li>96-Well-Plate</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_96_well_plate_9a66005814.jpg" alt="Phenomenex: Strata X 96-well plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_96_well_plate_9a66005814.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>1, 3, and 6mL Tubes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg" alt="Phenomenex: Strata X 1, 3, and 6mL Tubes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_1_9b04cab6c1.jpg 156w," sizes="100vw" width="1080" height="1080"><ul><li>Giga Tubes (12-150mL Tubes)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg" alt="Phenomenex: Strata X Giga Tubes (12-150mL Tubes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_tubes_2_a8cd1706ec.jpg 156w," sizes="100vw" width="1080" height="1080">

Phenomenex Biozen N-Glycan Clean-Up

Biozen N-Glycan Clean-up enables fast, efficient SPE purification of labeled, released N-glycans using HILIC microelution, ideal for small sample volumes and LC-MS workflows.

<h4>Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)</h4>SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.<ul><li>Small volume format allows for more concentrated samples</li><li>Minimal elution solvent</li><li>Excellent retention and recovery of labeled, released N-glycans</li><li>Faster clean-up methods to save on sample prep time</li></ul><h4>Protocol</h4><ul><li>96-Well Plate: Biozen N-Glycan Clean-up</li><li>Part No.: 8M-S009-NGA</li><li>Condition: 200 µL Water</li><li>Equilibrate: 200 µL Water/Acetonitrile (15:85)</li><li>Load: Labeled Sample (up to 400 µL of labeled sample)</li><li>Wash: 2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)</li><li>Elute: 3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)</li><li>Dilute: 100 µL Dimethylformamide and then 210 µL of Acetonitrile</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_N_Glycan_962a23eee6.jpg" alt="Phenomenex: Biozen N-Glycan Clean-up" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_N_Glycan_962a23eee6.jpg 245w," sizes="100vw" width="1444" height="424">

Phenomenex Clarity OTX Clean-Up

Rapid 15-minute clean-up of biological samples using Clarity OTX SPE enables efficient isolation of RNA- and DNA-based therapeutics without LLE, optimized for LC-MS bioanalysis.

<h4>15 Minute Clean Up from Biological Samples</h4>Isolate RNA and DNA-based therapeutics / metabolites from biological fluids and tissues in less time with Clarity OTX solid phase extraction (SPE) and buffer products.<ul><li>No liquid-liquid extraction (LLE) required required</li><li>&gt;80 % typical extraction recoveries</li><li>Optimized for LC-MS bioanalysis</li></ul><h3><app-cb-header style="color:rgb(65, 66, 69);font-family:proxima-nova, sans-serif;font-size:16px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:left;text-indent:0px;text-transform:none;"><div><div><h2 style="border-bottom:1px solid rgb(51, 51, 51);color:rgb(0, 41, 72);font-size:32px;font-style:normal;font-weight:400;line-height:1.2;"><div>General Extraction</div></h2></div></div></app-cb-header><app-cb-fourtext-eightimage style="color:rgb(65, 66, 69);font-family:proxima-nova, sans-serif;font-size:16px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:left;text-indent:0px;text-transform:none;"><div><div><div style="flex-wrap:wrap;"><div style="flex:0 0 100%;"><h2 style="color:rgb(0, 41, 72);font-size:32px;font-weight:400;line-height:1.2;">Common Extraction Procedure (~180 minutes)</h2></div></div></div></div></app-cb-fourtext-eightimage>General Extraction</h3><h4>Common Extraction Procedure (~180 minutes)</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Clarity_OTX_Clean_Up_e317411382.jpg" alt="Phenomenex: Clarity OTX Clean-Up: Common Extraction Procedure (~180 minutes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Clarity_OTX_Clean_Up_e317411382.jpg 245w," sizes="100vw" width="678" height="374"><h5>Suitable for Most Oligo Therapeutics &amp; Samples</h5>Oligo types / Sample Types<ul><li>DNA: Plasma</li><li>Aptamers: Serum</li><li>RNAi/siRNA: Urine</li><li>Thioates: Tears</li><li>Lipid-conjugates: Saliva</li><li>Liposome encapsulated: Tissue</li></ul><h4>Extraction Protocol</h4>Sample Pre-Treatment<ul><li>Tissue:<ul><li>Proteinase K digestion or homogenize</li><li>Mix 1-to-1 with Lysis-Loading buffer</li></ul></li><li>Biological Fluids: Mix 1-to-1 with Lysis-Loading buffer</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Clarity_OTX_Clean_Up_2_fd322c63cd.jpg" alt="Phenomenex: Clarity OTX Clean-Up: Extraction Protocol" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Clarity_OTX_Clean_Up_2_fd322c63cd.jpg 245w," sizes="100vw" width="1031" height="494">

Thermo Scientific QuEChERS Sample Preparation Kits

QuEChERS is a versatile sample preparation method for analyzing pesticides, veterinary drugs, and other residues in various sample types before GC-MS or LC-MS analysis.

<div><div><div><h3>QuEChERS sample preparation—now easier, more convenient, and affordable</h3>QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe)is a sample preparation method that was initially developed in 2003 for analyzing veterinary drugs in food. The method was also tested for pesticide residue analysis in plant material with great success. In 2003, the detailed method was published in the journal AOAC International. Since then, the QuEChERS method has evolved into a sample preparation technique used on a variety of analytes in an expanding number of sample types before analysis by <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/gas-chromatography-mass-spectrometry-gc-ms.html">GC-MS</a> or <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms.html">LC-MS</a>.Thermo Scientific QuEChERS kits contain pre-packaged, ready-weighed salts, sorbents, and buffers to streamline your workflow and minimize the potential for error. The kits are grouped according to your analytes of interest, sample matrix, and preferred QuEChERS method:<ul><li>Original 2003 Method</li><li>AOAC 2007.01 Method</li><li>EN 15662 Method</li><li>NY/T 1380-2007 Method</li></ul>Complete packages—including both extraction and clean-up kits—are available. Extraction-only and cleanup-only kits are also offered to suit your laboratory’s sample preparation needs. These pre-packaged kits save time and money, make QuEChERS procedures easier and more convenient, and ensure quality to yield reproducible results and excellent recoveries for a wide variety of analytes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg 217w," sizes="100vw" width="891" height="640"><h3>Typical QuEChERS workflow</h3><div><div><div><div><div>As shown in the workflow diagram below, the QuEChERS method has four basic steps:<ol><li>Homogenize sample (fruit or vegetable)</li><li>Extract analytes of interest from a sample using extraction salts (pesticide residues, veterinary drugs, and/or mycotoxins)</li><li>Clean-up the resulting supernatant using dispersive solid phase extraction (dSPE)</li><li>Analyze by GC-MS or LC-MS</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg 245w," sizes="100vw" width="1170" height="706"><div><h3>QuEChERS products</h3></div><div><h4>QuEChERS extraction kits for original 2003 method</h4></div><div>The original QuEChERS method (Anastassiades et al, 2003) was developed by Steven J. Lehotay and Michelangelo Anastassiades in 2003 for the determination of pesticide residues in fruits and vegetables. The original method was created to analyze non-base-sensitive compounds and uses a sodium chloride buffer to reduce polar interferences and enhance extraction. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. Three different extraction kits are available to support the original 2003 QuEChERS method.<h5>Chemicals used in the original 2003 method</h5></div><ul><li>Acetonitrile: Used to perform single-phase extraction (SPE) on a sample</li><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>NaCl: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample; reduces polar interference and enhances extraction</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li></ul><div><h4>QuEChERS extraction and clean-up kits for AOAC 2007.01 method</h4>The AOAC 2007.01 QuEChERS Method was developed by Steven J. Lehotay and his co-workers, who modified the original 2003 method to accommodate the use of acetate buffering at higher concentration for greater buffering strength. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix. These extraction and clean-up products here support the AOAC 2007.01 method.<div><h5>Chemicals used in the AOAC 2007.01 method</h5><ul><li>Acetonitrile: Used to perform single-phase extraction on a sample</li><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>NaOAc: Buffers the sample to stabilize pH</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li>C18: Removes fats, sterols, and other non-polar interferences from sample</li><li>GCB: Graphitized carbon black, removes pigment (not recommended for use with planar pesticides)</li></ul><div><h4>QuEChERS extraction and clean-up kits for EN 15662 method</h4>The extraction and clean-up products here support the EN 15662 method. Widely used in Europe, the EN 15662 method was developed by Michelangelo Anastassiades and co-workers, who modified the original 2003 method to accommodate the use of citrate buffering at a relatively low buffering capacity. This variation uses a sodium chloride buffer to minimize polar interferences in addition to sodium citrate dihydrate and disodium citrate sesquihydrate buffers. Buffering is an important part of the QuEChERS procedure because it allows for analysis of pesticides and other residues that display pH dependence in their recoveries, depending on the sample matrix.<h5>Chemicals used in the EN 15662 method</h5><ul><li>Acetonitrile: Used to perform single-phase extraction on a sample</li><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>NaCl: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample</li><li>Trisodium Citrate: Buffers the sample to stabilize pH</li><li>Disodium Citrate Sesquihydrate: Buffers the sample to stabilize pH</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li>C18: Removes fats, sterols, and other non-polar interferences from sample</li><li>GCB: Graphitized carbon black, removes pigment (not recommended for use with planar pesticides)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg 245w," sizes="100vw" width="1471" height="488"><div><h4>QuEChERS clean-up kit for NY/T 1380-2007 method</h4></div><div>The NY/T 1380-2007 method is recommended by China Ministry of Agriculture for the QuEChERS Method sample preparation of 51 specific pesticide residues in fruits and vegetables with analysis by GC-MS.<h5>Chemicals used in the NY/T 1380-2007 method</h5><ul><li>Anhydrous MgSO₄: Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li>PSA: Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dSPE step</li><li>C18: Removes fats, sterols, and other non-polar interferences from sample</li></ul></div></div></div></div></div></div></div></div></div></div></div></div>

Products from category SPE sample preparation - page 1

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