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Manufacturer

Phenomenex

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories. From drug discovery and pharmaceutical development to disease diagnosis, food safety to environmental analysis, Phenomenex chromatography solutions accelerate science and help researchers improve global health and well-being.

Distributor

Phenomenex

(310) 212-0555 USA

SPE sample preparation

Phenomenex Clarity OTX Clean-UpRapid 15-minute clean-up of biological samples using Clarity OTX SPE enables efficient isolation of RNA- and DNA-based therapeutics without LLE, optimized for LC-MS bioanalysis.

Features

Phenomenex Clarity OTX Clean-Up

15 Minute Clean Up from Biological Samples

Isolate RNA and DNA-based therapeutics / metabolites from biological fluids and tissues in less time with Clarity OTX solid phase extraction (SPE) and buffer products.

No liquid-liquid extraction (LLE) required required
>80 % typical extraction recoveries
Optimized for LC-MS bioanalysis

General Extraction
Common Extraction Procedure (~180 minutes)
General Extraction

Common Extraction Procedure (~180 minutes)

Phenomenex: Clarity OTX Clean-Up: Common Extraction Procedure (~180 minutes)

Suitable for Most Oligo Therapeutics & Samples

Oligo types / Sample Types

DNA: Plasma
Aptamers: Serum
RNAi/siRNA: Urine
Thioates: Tears
Lipid-conjugates: Saliva
Liposome encapsulated: Tissue

Extraction Protocol

Sample Pre-Treatment

Tissue:
- Proteinase K digestion or homogenize
- Mix 1-to-1 with Lysis-Loading buffer
Biological Fluids: Mix 1-to-1 with Lysis-Loading buffer

Phenomenex: Clarity OTX Clean-Up: Extraction Protocol

Manufacturer

Phenomenex

Distributor

Phenomenex

(310) 212-0555 USA

www.phenomenex.com

[email protected]

News

Article | Academy

SPE Troubleshooting - Extra Peak

An SPE technical tip focused on identifying the sources of interferences and extra peaks observed after sample extraction.

Phenomenex

Article | Academy

Why are my recoveries greater than 100%?

Although 100% recovery is expected in SPE, values above 100% are sometimes observed. This article explains the most common causes and provides practical guidance on how to identify and resolve them.

Phenomenex