<div><div><div><div><h3><strong>How Does Your Monthly Nitrogen Gas Bill Compared to Other Labs?</strong></h3><p><span><strong>If your lab relies on nitrogen gas cylinders, you already know how quickly costs can add up. Between </strong></span><span style="font-weight:normal;"><strong>purchase prices, rental fees, delivery charges, and frequent refills</strong></span><span><strong>, </strong></span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/understanding-the-costs-of-renting-nitrogen-gas-cylinders-in-laboratories?hsLang=en"><span><strong>nitrogen cylinders can become a </strong></span><span style="font-weight:normal;"><strong>hidden financial drain</strong></span></a><span><strong> on your operations.</strong></span></p><p><span>But how do you know if you're paying a fair price—or if you're overspending compared to other labs? Our </span><i><span style="font-weight:normal;">"Am I Overpaying on my Nitrogen Gas Cylinders?"</span></i><span> tool helps you determine whether your current nitrogen supply costs are within an acceptable range or if you're paying too much.</span></p><h3><strong>The Hidden Costs of Nitrogen Cylinders</strong></h3><p><span>Nitrogen cylinders are a staple in many laboratories, but they come with several costly drawbacks:</span></p><h4><strong>1. High Cylinder Costs</strong></h4><p><span>The upfront cost of nitrogen cylinders can be significant, especially for labs that require multiple cylinders per month. Pricing varies depending on size and supplier, but the recurring need to purchase or replace cylinders creates an ongoing expense that can quickly add up. Unlike a fixed investment in a nitrogen generator, cylinder costs are a </span><span style="font-weight:normal;">constant and unavoidable</span><span> part of lab operations.</span></p><h4><strong>2. Monthly Rental Fees</strong></h4><p><span>Most gas suppliers charge a rental fee per cylinder, adding to the overall cost of maintaining a steady nitrogen supply. These fees might seem small at first, but when multiplied across multiple cylinders and months, they become a substantial recurring expense. Over time, these rental costs can easily surpass the price of investing in an in-house nitrogen generation system.</span></p><h4><strong>3. Delivery &amp; Handling Expenses</strong></h4><p><span>Delivery costs vary but can </span><span style="font-weight:normal;">add hundreds of dollars per month</span><span> to your nitrogen expenses, especially if you're receiving frequent shipments. Beyond costs, frequent deliveries create </span><span style="font-weight:normal;">workflow disruptions </span><span>and </span><span style="font-weight:normal;">safety concerns</span><span> due to cylinder handling and storage. Each delivery requires time, coordination, and often additional fees, making cylinders an inefficient option for labs that require a reliable nitrogen supply.</span></p><h4><strong>4. Gas Waste</strong></h4><p><span>Did you know that most nitrogen cylinders leave </span><span style="font-weight:normal;">about 10% of their gas unused </span><span>due to pressure limitations? That means you're </span><span style="font-weight:normal;">paying for nitrogen you can’t even use</span><span>. Additionally, each time a cylinder runs empty, there is downtime involved in switching to a new one, causing further inefficiencies in lab operations.</span></p><h4><strong>5. Storage &amp; Safety Risks</strong></h4><p><span>Cylinders require dedicated storage space and proper handling to </span><span style="font-weight:normal;">prevent leaks, pressure hazards, and accidents</span><span>. Many labs struggle with cylinder management, leading to </span><span style="font-weight:normal;">compliance concerns and workflow inefficiencies</span><span>. Storing multiple high-pressure cylinders also introduces potential safety risks, requiring strict adherence to safety protocols and regulatory standards.</span></p><h3><strong>The Smarter Alternative: Switching to a Nitrogen Generator</strong></h3><p><span>A </span><span style="font-weight:normal;">nitrogen generator</span><span> offers a </span><span style="font-weight:normal;">cost-effective, on-demand</span><span> solution for labs that need a steady nitrogen supply—without the ongoing expenses of cylinder rentals, refills, and deliveries. Here’s why more labs are making the switch:</span></p><ul><li><span><strong>Eliminate Cylinder Costs</strong> – No more purchases, rentals, or refills.</span></li><li><span><strong>Reduce Long-Term Expenses</strong> – A one-time investment with minimal maintenance.</span></li><li><span><strong>On-Demand Supply</strong> – Generate </span><span style="font-weight:normal;">high-purity nitrogen</span><span> whenever you need it.</span></li><li><span><strong>No More Deliveries</strong> – Say goodbye to disruptions and recurring delivery fees.</span></li><li><span><strong>Increased Safety &amp; Efficiency</strong> – No handling of high-pressure cylinders.</span></li><li><span><strong>Better Environmental Impact</strong> – </span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/are-nitrogen-generators-actually-more-environmentally-friendly?hsLang=en"><span>Reduce carbon emissions</span></a><span> associated with cylinder deliveries and production.</span></li></ul><p><span>Many labs find that a </span><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/would-a-nitrogen-generator-make-sample-preparation-more-affordable?hsLang=en"><span style="font-weight:normal;">nitrogen generator pays for itself within a year</span></a><span>—especially if they use multiple cylinders per month. Beyond the financial savings, labs that </span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/why-nitrogen-generators-are-gaining-popularity?hsLang=en"><span>switch to a nitrogen generator</span></a><span> experience </span><span style="font-weight:normal;">greater efficiency, safety, and reliability</span><span> in their daily operations.</span></p><blockquote><p><span><strong>Learn More: </strong></span><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/nitrogen-generators-for-sample-preparation-everything-you-need-to-know?hsLang=en"><span><strong>Nitrogen Generators for Sample Preparation: Everything you need to know</strong></span></a></p><p><span><strong>Watch Our Webinar: </strong></span><a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/the-case-for-nitrogen-generation-in-your-lab?hsLang=en"><span><strong>Improving Laboratory Workflows with Nitrogen Generation</strong></span></a></p></blockquote><h3><strong>Find Out If You’re Overpaying</strong></h3><p><span>Not sure if switching to a nitrogen generator makes sense for your lab? Use our </span><i><span style="font-weight:normal;">"Am I Overpaying on My Nitrogen Gas Cylinders?"</span></i><span> tool to compare your costs against industry benchmarks. Simply answer a few quick questions, and we’ll help you determine whether it’s time to make the switch.</span></p></div></div></div></div>

Am I Paying Too Much for my Nitrogen Gas Cylinders?

<p><strong>Intensive farming, even when good agricultural practices are followed, very often results in pesticide contamination of soil, surface water and groundwater. Water sources used for drinking water production are thus often affected. The outcome is the progressive banning of proven, effective, yet persistent pesticides in the environment, alongside a decline in water quality. This, in turn, results in substantial investments in water treatment technologies to ensure that drinking water meets relevant legal standards. Passive, easy to maintain and low-cost in-situ denitrification woodchip bioreactors might be a solution to this situation. These bioreactors have already been successfully used in some countries (e.g. the USA) to remove other types of agricultural pollution directly at the point of origin, thus preventing its further transport in the environment.&nbsp;</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_ba53628b5a.jpg" alt="ALS Czech Republic: EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape: Figure 1: A real example of an installed semi-operational bioreactor unit." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_ba53628b5a.jpg 245w," sizes="100vw" width="1971" height="919"></p><h3><strong>Nitrates and Denitrifying Bioreactors</strong></h3><p>Denitrifying woodchip bioreactors are a relatively simple containerised treatment technology originally intended for the removal of nitrates from agricultural outflows. They use woodchip media as a source of bioavailable organic carbon to promote denitrification, which converts nitrates to gaseous nitrogen, the predominant natural component of the atmosphere. The advantages of using denitrification woodchip bioreactors are low acquisition and operating costs, minimal maintenance, long lifetime, and low land take. At some sites, these bioreactors have been operated for more than 15 years without major interventions and with the original fill and still achieve high denitrification rates.</p><h3><strong>ALS Research Project</strong></h3><p>To verify the usability of denitrifying woodchip bioreactors for the simultaneous removal of both nitrates and pesticides found in surface runoff from agriculturally active areas is the goal of research project "Comprehensive assessment of soil contamination with pesticides and in-situ remediation leading to eliminate their entry into groundwater“. The project is being realised under the leadership of ALS Laboratories in cooperation with other research institutes. Work on the project will continue until 2025.</p><h3><strong>Semi-operational Unit and Expected Pesticides Removal</strong></h3><p>Previous research projects by ALS and their partners have thoroughly mapped and identified sites that have been, in terms of pesticides occurrence, problematic for a long time. A semi-operational unit of the denitrification woodchip bioreactor described above was commissioned at one of these sites in the autumn of 2023.</p><p>The unit uses poplar wood chips as a feedstock, which have proven well for this purpose, and is equipped with automatic samplers, sensors measuring basic physical and chemical parameters and a data station with on-line data transmission.</p><p>In the case of pesticides, several decontamination mechanisms are envisaged in the bioreactor area, the most important of which will be the sorption and microbial decomposition. The aim of the project is to suggest the operating conditions of the bioreactor in such a way that the natural processes of microbial degradation of pesticides are facilitated to the maximum extent possible, or to support these processes by active intervention in the physicochemical composition of the bioreactor and its microflora.</p><blockquote><p><strong>Related EnviroMail_08_Europe:</strong><br><a target="_blank" rel="noopener noreferrer" href="https://www.alsglobal.eu/enviromails/enviromail_08"><strong>Hidden Pesticide Threats in Protected Landscape Areas</strong></a></p></blockquote><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_2_4e7e587035.jpg" alt="ALS Czech Republic: EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape: Figure 2: Results of pesticides detected in tested soil samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_2_4e7e587035.jpg 245w," sizes="100vw" width="1439" height="783"></p><h3><strong>Case Study: Monitoring of Pesticides in Soil Sam- ples from Central Europe</strong></h3><p>In June 2023, monitoring of pesticides in agricultural soils and surrounding surface waters was conducted from 45 sites across Central Europe. The range of determined targeted substances was based on prepared risk analysis taking into account the consumption of pesticides and their risk properties such as toxicity and persistence. Analytical methods based on liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were used to determine a wide range of active pesticide substances and their metabolites (308 and 352 parameters, respectively). Results are summarized in <strong>Figure 2</strong>.</p><p>Pesticides were detected in all collected soil samples ranging from 0.01 to 0.73 mg/kg DW (dry weight of soil). By far the most abundant group was glyphosate pesticides, i.e. the herbicide glyphosate and especially its metabolite AMPA (Aminomethylphosponic acid), its total concentrations ranged from 0.01 to 0.46 mg/ kg DW in 36 of the 45 samples collected and tested. Comparing to levels and incidence of pesticides within the Europe, glyphosate and AMPA belong to the most frequently detected and those with the highest concentrations pesticide compounds generally.</p><p>In related surface water samples, pesticides were detected in 43 out of 45 tested samples, their concentrations ranged from 0.01 μg/L to 14.1 μg/L. The most abundant pesticides detected were chloracetanilide and glyphosate.</p><h3><strong>Protective Measures in the Landscape</strong></h3><p>Based on the results obtained during the testing of the semi-operational denitrification woodchip bioreactor unit, a linear measure will be designed and properly sized for the model catchment to remove pesticides directly at the point of generation and thus prevent their further spread.</p><h3><strong>European Legislation</strong></h3><ul><li><strong>SOIL:</strong> Proposal COM(2023) 416 final of 5 July 2023 for a directive of the European Parliament and of the Council on Soil Monitoring and Resilience (Soil Monitoring Law).</li><li><strong>SURFACE WATER:</strong> Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on environmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 2000/60/EC of the European Parliament and of the Council.</li><li><strong>GROUND WATER: </strong>Directive 2006/118/EC of the European Parliament and of the Council of 12 December 2006 on the protection of groundwater against pollution and deterioration.</li><li><strong>DRINKING WATER: </strong>Directive (EU) 2020/2184 of the European Parliament and of the Council of 16 December 2020 on the quality of water intended for human consumption (recast).</li></ul>

EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape

<p><strong>Mushrooms – a fragrant forest treasure for some, a fascinating object of study for others. In this case, the connection between nature and science is entirely natural. What can you discover about a simple mushroom under a microscope or using chromatography? And how helpful is a scientific education when it comes to everyday mushroom hunting?&nbsp;</strong></p><p>We asked <strong>RNDr. Roman Kysilka, CSc.</strong>, who worked as a research specialist at the Czechoslovak Academy of Sciences and now works at <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"><strong>Watrex Prague</strong></a>. He walked us through not only his career, but also through the forest – both scientific and real.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_19_6_uvod_1600_X900_4dca2a04b3.jpg" alt="Watrex Fig.1: RNDr. Roman Kysilka, CSc." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_19_6_uvod_1600_X900_4dca2a04b3.jpg 245w," sizes="100vw" width="3540" height="1875"></p><h4><strong>When and why did you decide to study natural sciences? Were you drawn to them as a child?</strong></h4><p>I got a chemistry experiment kit, I think it was called "Curious Chemist," around seventh grade, and I was immediately captivated by home experiments. I was also into electricity and experimented with that as well. In my early years of high school, I was still considering studying electrical engineering or chemistry. But chemistry won out, largely thanks to the chemistry olympiads I took part in regularly from elementary school. Through national competitions (later in high school), I met a lot of chemistry enthusiasts. And during training camps for the international olympiad, I got to know chemists from the Faculty of Science at Charles University – and that sealed the deal.</p><h4><strong>How did you first get into studying mushrooms? Was it by chance or intentional?</strong></h4><p>It was a coincidence. I did grow champignons when I was around twelve, but that had nothing to do with research – I was mostly curious about what my mom would cook with them. Between 1982 and 1984, I was working on my thesis, and my advisor, Prof. Karel Štulík, arranged for me to work in a chromatography lab in the Department of Experimental Mycology at the Institute of Microbiology of the Czechoslovak Academy of Sciences. And that’s where I first encountered mushrooms as a subject of scientific research.&nbsp;</p><h4><strong>What fascinated you most about mushrooms?&nbsp;</strong></h4><p>Mushrooms have been with humankind since ancient times. You might recall the phrase “mushrooms – meat of the poor.” The Aztecs used the word teonanácatl, which roughly translates as “meat of the gods” (later found to refer to mushrooms with psychoactive effects). Meat for both the poor and the gods – interesting, right? The diverse world of fungi – heterotrophic organisms with a vital role in nature – fascinated me from the start. And of course, there’s also the unexpected potential of the compounds mushrooms produce. Every year, hundreds of new, previously unknown chemical compounds – secondary metabolites – are isolated and described in scientific literature, with potential uses in medicine and other fields. You could say the world of fungi is still largely unexplored from a chemical standpoint.&nbsp;</p><h4><strong>You worked at the Czechoslovak Academy of Sciences from 1985–1991 – what exactly did you do there?&nbsp;</strong></h4><p>After returning from military service, I went back to the Institute of Microbiology, where I focused on research into psychoactive tryptamine derivatives in basidiomycetes for my dissertation. The list of substances later narrowed to psilocybin, psilocin, baeocystin, and bufotenin. I worked on developing analytical methods to detect these compounds in fruiting bodies and mycelium, designing chromatographic methods for semi-preparative scale isolation, conducting cultivation experiments with various Psilocybe species in the lab, and screening potentially psychoactive mushrooms for these compounds.&nbsp;</p><h4><strong>Do you recall a moment or discovery that left a strong impression on you?&nbsp;</strong></h4><p>Yes. Part of the research on magic mushrooms included experimental poisonings. In the late 1980s, together with Marta Semerdžieva, we completed (and later patented) a method for producing mushroom mycelium via submerged cultivation. We planned an intoxication experiment using this rather unappealing material. Based on HPLC analysis, we cooked a “mushroom” soup and began the experiment. However, the outcome was very different from what we expected – it was clear we had significantly overdosed the test subjects. That prompted a detailed review of the analytical method used to determine psilocybin and psilocin levels. We found that the critical step for accurate results was the sample preparation before HPLC analysis. With optimized extraction, we detected concentrations of active compounds several times higher than with previously published methods. Our article in Planta Medica is still cited today and occasionally discussed in forums about psychoactive substances.&nbsp;</p><h4><strong>What techniques were you using then – and what surprises you most about modern lab methods?&nbsp;</strong></h4><p>Our main analytical technique was HPLC. Mass spectrometry, which is standard today, was just a dream back then. The peak was using a UV-VIS diode-array detector or selective and sensitive amperometric detection. I find the current development of lab automation, the explosive growth of computing and mathematical modeling, and more recently, the use of artificial intelligence truly remarkable.</p><h4><strong>How did you transition from academia to Watrex Prague? What attracted you to the change?</strong></h4><p>In the early '90s, research projects at the Institute of Microbiology were being reorganized, and several colleagues I worked with left the institute. Around the same time, I got a job offer from Milan Minárik, who was founding <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"><strong>Watrex</strong></a> – a distributor for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/325"><strong>Waters</strong></a> chromatography systems. I had met Milan a few years earlier (through HPLC, of course), and the idea of working as an applications specialist in his private company was very appealing. It was a time of rapid private sector growth in Czechoslovakia, and I was excited to be a part of that.&nbsp;</p><h4><strong>You’ve studied mushrooms scientifically, but what about in the actual forest? Are you a mushroom picker?</strong></h4><p>I love picking mushrooms (their fruiting bodies), but I don’t have as much time for it as I’d like.&nbsp;</p><h4><strong>How much do you rely on your scientific knowledge when mushroom picking? Do you see mushrooms differently than the average picker?&nbsp;</strong></h4><p>Rather than scientific knowledge, I rely on experience from field excursions with the Institute of Microbiology, where I had the chance to learn mushroom identification from leading Czech mycologists. And unlike “typical pickers,” I often collect gilled mushrooms. My three-year-old granddaughter Anička might actually be able to name more bolete species than I can.</p><h4><strong>Does your knowledge of chemistry help with identifying lesser-known species?</strong></h4><p>No.&nbsp;</p><h4><strong>Do you have a favorite mushroom – either for eating or just because it’s interesting?&nbsp;</strong></h4><p>Aside from magic mushrooms, I have a soft spot for the fly agaric. Amanita muscaria, with its unmistakable red cap and white spots, has a rich and ancient history. Its story intersects with folk medicine and shamanism across many cultures, especially in the Northern Hemisphere. Another mushroom worth mentioning is the deadly Cortinarius orellanus. It grows in our region and can unfortunately be confused with some edible species. It contains the nephrotoxin orellanine, which is dangerous due to its delayed onset – symptoms can appear up to three weeks after ingestion, which makes treating the poisoning extremely difficult.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_19_6_obr_2_02794e1777.jpg" alt="Watrex Fig.2: Illustrative photo of Amanita muscaria and Cortinarius orellanus" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_19_6_obr_2_02794e1777.jpg 245w," sizes="100vw" width="1600" height="839"></p><h4><strong>What can we learn about a mushroom in a laboratory — apart from whether it’s edible or not?&nbsp;</strong></h4><p>A mushroom, as a subject of laboratory study, can be analyzed taxonomically; we can identify substances that are toxicologically significant, search for new biologically active compounds, or use fungi as model organisms in genetic research. Their bioaccumulation capacity can help monitor unwanted substances in the environment. And I’m sure there are many other possibilities for scientific investigation in the lab.&nbsp;</p><h4><strong>Have you ever examined the presence of toxins, heavy metals, or pesticides? How common are they in mushrooms?&nbsp;</strong></h4><p>The ability of mushrooms to accumulate foreign substances is well documented and stems from the way they absorb nutrients from their surroundings. Their mycelium has a huge surface area and absorbs not only nutrients from the soil solution, but also unwanted substances, which may then accumulate in the fruiting bodies. I personally haven’t focused on bioaccumulation research in fungi — with one exception. After the Chernobyl nuclear disaster, we collected various mushroom species to monitor levels of Cesium-137.&nbsp;</p><h4><strong>What’s your opinion on identifying mushrooms using mobile apps?&nbsp;</strong></h4><p>If a mobile app serves as a replacement for a quality, well-illustrated mushroom atlas, I have no objections. However, I’d be very cautious when using various "identification keys." An amateur can easily overlook or misinterpret an important feature that differentiates an edible mushroom from an inedible or even poisonous one. That said, with the advancement of AI, things will surely improve :). As always — only collect mushrooms you can identify with absolute confidence!&nbsp;</p><h4><strong>What’s something every mushroom hunter should know — but often overlooks?</strong></h4><p>Where north is — and how to get back to the train station or their parked car.</p><h4><strong>Besides a knife, is there anything else you’d bring in your mushroom basket?&nbsp;</strong></h4><p>A camera. Mushrooms are incredibly photogenic. And if one doesn't end up in the basket, it can at least become a model for photography.&nbsp;</p><h4><strong>And finally: If you had to describe mushrooms in one word, what would it be?</strong></h4><p>Saprophytes. Without them, life on our planet would come to a halt.&nbsp;</p><p><i><strong>Many thanks to Roman Kysilka for such an open and inspiring conversation. It was a pleasure to explore his professional journey and the unique experiences that have shaped it. Roman currently works at </strong></i><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"><i><strong>Watrex Praha</strong></i></a><i><strong>, where his expertise and experience continue to make a valuable impact. We wish him all the best in his future professional and personal endeavors!</strong></i></p>

Are you scientifically prepared for the new mushroom-picking season?

Article

Product

Scientists

Environment

Video

For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

ALS Czech Republic

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Phenomenex Strata SAX Solid Phase Extraction (SPE)

The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.

<p><strong>Ion-Exchange Solid Phase Extraction Sorbent (SPE)</strong></p><p>The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.</p><figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_sax_470d923b27.webp" alt="Phenomenex: Strata-SAX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_sax_470d923b27.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li><strong>Surface Area (m²/g)</strong>: -</li><li><strong>Separation Mode:</strong> Ion Exchange</li><li><strong>Particle Size:</strong> 55</li><li><strong>Recommended Use: </strong>Extraction of weakly acidic compounds</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>Strata SAX – Strong Anion-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL DI Water</li><li><strong>Load:</strong> Pretreated sample, pH 6-7</li><li><strong>Wash 1</strong>: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li><strong>Wash 2</strong>: 1 mL Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5% Formic Acid in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Phenomenex Strata Screen-A Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.

<p><strong>Mixed-Mode Solid Phase Extraction (SPE) Sorbent</strong></p><p>Incorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.</p><figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_A_f370bdfd48.webp" alt="Phenomenex: Strata Screen-A" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_A_f370bdfd48.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li><strong>Surface Area (m²/g)</strong>: -</li><li><strong>Separation Mode:</strong> Multi-Mode</li><li><strong>Particle Size:</strong> 55</li><li><strong>Recommended Use: </strong>Extracts acidic drugs from biological matrices</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>Strata Screen-A Strong Anion-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL Water</li><li><strong>Load:</strong> Pretreated sample</li><li><strong>Wash 1</strong>: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li><strong>Wash 2</strong>: 1 mL Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5% Formic Acid in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Phenomenex Strata Screen-C Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.

<p><strong>Strong Cation-Exchange Solid Phase Extraction (SPE)</strong></p><p>Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.</p><figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_C_b60bb2b7e4.webp" alt="Phenomenex: Strata Screen-C" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_C_b60bb2b7e4.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li><strong>Surface Area (m²/g)</strong>: 500, m²/g, 500 m²/g</li><li><strong>Separation Mode:</strong> Multi-Mode</li><li><strong>Pore Size</strong>: 70</li><li><strong>Particle Size:</strong> 55</li><li><strong>Recommended Use: </strong>Extraction of basic drugs from biological matrixes</li></ul><h4><strong>Starting Method</strong></h4><h5><strong>Strata Screen-C Strong Cation-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL Acidified Water</li><li><strong>Load:</strong> Pretreated sample (acidified)</li><li><strong>Wash 1</strong>: 1 mL 0.1N HCl in Water</li><li><strong>Wash 2</strong>: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5 % NH<sub>4</sub>OH in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Phenomenex Strata Screen-C GF Solid Phase Extraction (SPE)

Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs.

<p><strong>Strong Cation-Exchange Solid Phase Extraction (SPE)</strong></p><p>Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs from biological matrixes. Offers the selectivity of Screen-C in a gravity flow particle size for viscous samples.</p><h4><strong>Starting Method</strong></h4><h5><strong>Strata Screen-C GF Strong Cation-Exchange</strong></h5><ul><li><strong>Condition:</strong> 1 mL Methanol</li><li><strong>Equilibrate:</strong> 1 mL Acidified Water</li><li><strong>Load:</strong> Pretreated sample (acidified)</li><li><strong>Wash 1</strong>: 1 mL 0.1N HCl in Water</li><li><strong>Wash 2</strong>: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li><strong>Elute: </strong>1 mL 5 % NH<sub>4</sub>OH in Methanol</li></ul><p>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.</p>

Watrex BioWorks WorkBeads resins and GoBio Mini columns

Watrex Praha is the official distributor of Bio-Works, a leading global manufacturer of chromatographic resins designed for the purification of biopharmaceuticals.

<p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Watrex Praha</strong></a> is the official distributor of Bio-Works, a leading global manufacturer of chromatographic resins designed for the purification of biopharmaceuticals. This partnership brings high-quality products to the Czech market, widely used in biotechnology and the pharmaceutical industry.</p><p>The main product of Bio-Works is <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads </strong></a>resins, manufactured using a patented agarosebased method. These resins are characterized by excellent mechanical stability, uniform bead size distribution, and high separation efficiency. They are available both as loose resins and in prepacked columns, making them easy to use in various applications.</p><p>For efficient small-scale purification and process development screening, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio Mini</strong></a> columns are the ideal solution. These prepacked columns are available in 1 ml and 5 ml sizes, facilitating quick and effective biomolecule separations.</p><p>Bio-Works products are used in bioprocess solutions focused on the purification and analysis of biomolecules, including monoclonal antibodies, proteins, peptides, oligonucleotides, viruses, and vaccines. These technologies offer top-tier quality and precision, ensuring optimal capacity and product purity.</p><h3><strong>Types of sorbents:</strong></h3><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/affinity-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Affinity chromatography resins</strong></a></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/affinity-chromatography/affinity-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads™ affimAb</strong></a> is an optimized alkali-stable protein A resin designed for purification of monoclonal- and polyclonal antibodies in laboratory to process scale. This resin has a superior base matrix combined with a patented, alkali-stable protein A ligand, which results in high dynamic binding capacity at short residence times. This resin binds over 40 mg IgG/mL resin also at as short residence time as 2.5 min. Available also in prepacked 1 mL and 5 mL <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/affinity-chromatography/affinity-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio™ Mini </strong></a>columns.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/ion-exchange-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Ion exchange chromatography (IEX) resins</strong></a></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/ion-exchange-chromatography/iex-sorbents/"><strong>WorkBeads™ resins for ion exchange chromatography</strong></a> are designed for research and industrial scale purification of proteins, peptides and oligonucleotides by utilizing the difference in their surface charge. These resins demonstrate high-resolution separation while giving low backpressure to facilitate both capture and polishing purification applications in standard bioprocess columns.</p><ul><li><strong>WorkBeads 100S</strong> is a strong cation exchanger and will bind positively charged substances.</li><li><strong>WorkBeads 100Q</strong> is a strong anion exchanger and will bind negatively charged substances.</li><li><strong>WorkBeads 40S</strong> is a strong cation exchanger with sulfonate ligands and will bind positively<br>charged substances.&nbsp;</li><li><strong>WorkBeads 40Q</strong> is a strong anion exchanger with quaternary amine and will bind negatively charged substances.&nbsp;</li><li><strong>WorkBeads 40 DEAE</strong> is a weak anion exchanger with tertiary amine ligands (diethylaminoethyl) and is an alternative to WorkBeads 40Q when looking for alternative selectivities.</li></ul><p>Also available in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/ion-exchange-chromatography/iex-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio™ Mini.</strong></a></p><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Size exclusion chromatography (SEC) resins</strong></a></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads™ 40/100 SEC, WorkBeads 40/1000 SEC, WorkBeads 40/10 000 SEC, and</strong></a><br><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads Macro SEC resins</strong></a> are used for preparative size exclusion chromatography (SEC) in laboratory and process scale purification of proteins, viruses, and other biomolecules by utilizing the differences in their size. These resins are based on agarose, which is a biopolymer suitable for separation of biomolecules. WorkBeads resins are cross-linked using a proprietary method that results in a very rigid structure. Although the general recommendation for SEC is to use a low flow rate for best purification, the rigidity and tight particle size distribution allow for the purification of viruses and other large substances at a high flow rate for fast processing and high yields.</p><p>Produced using a proprietary cross-linking method that results in highly porous and physically stable matrices.</p><p>We have recently added two new Size Exclusion Chromatography (SEC) products that expand the range for more choice and flexibility in separating large particles. WorkBeads 200 SEC, with 180 μm beads, is suitable for viscous samples like serum and whole blood. WorkBeads Macro SEC resins extend the range of several resins with a bead size of 45 μm but different porosities, making it easy to change between them when different fractionation ranges are desired.</p><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads Dsalt resin</strong></a> is designed to enable quick and easy separations of high- and lowmolecular-weight substances. This resin is pre-swollen and enables efficient desalting and buffer exchange of proteins, large peptides, and nucleic acids.</p><p>Also available in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/gel-filtration-sec/sec-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio™ Mini</strong></a>.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/hic-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Hydrophobic interaction chromatography (HIC) resins</strong></a></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/hic-chromatography/hic-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads™ 40 Butyl SH</strong></a> is a resin for hydrophobic interaction chromatography (HIC) designed for research and industrial scale purification of proteins, peptides, plasmids, and oligonucleotides by utilizing the difference in their surface hydrophobicity. It’s often used to complement other techniques that separate according to either charge or size.</p><p>The functional ligand of WorkBeads 40 Butyl SH is n-butyl thioether. Since butyl is a very<br>hydrophobic linear chain, minimal mixed-mode interactions are expected. The resin is optimized to offer reliable binding performance. The property of high-resolution separation, in combination with low backpressure, allows both capture and polishing purification applications in standard bioprocess columns. The resin is also available in several different ready-to-use prepacked column sizes, such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/hic-chromatography/hic-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio™ Mini</strong></a> 1 mL and 5 mL, GoBio Screen 7 x 100 (3.8 mL), GoBio Prep 16 x 100 (20 mL) and 26 x 100 (53 mL) as well as GoBio Prod columns starting at 1 L. 5.&nbsp;</p><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/imac-chromatography/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Immobilized metal-ion affinity chromatography (IMAC) resins&nbsp;</strong></a></h4><p>There are three different chelating ligands that <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/imac-chromatography/imac-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeads IMAC resins</strong></a> are based on. First are WorkBeads IDA (iminodiacetic acid) and WorkBeads NTA (nitrilotriacetic acid), which can be charged or are pre-charged with four different metals (Ni2+, Co2+, Cu2+, and Zn2+). Different combinations of ligands and metals give unique selectivities. Pre-charged IDA and NTA-based IMAC resins can be stripped, cleaned, and recharged with fresh metal ions.&nbsp;</p><p>The third resin is WorkBeads NiMAC, a nickel pre-charged resin with very low nickel leakage and tolerant against chelating agents (EDTA) and reducing substances (DTT). This resin is optimal for the purification of, e.g. eukarytic cell feeds, which often containing these substances.</p><p>For optimization, an initial screening is recommended with the different WorkBeads IMAC resins to identify the optimal combination of ligand and metal ion.</p><p>Also available in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/imac-chromatography/imac-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio™ Mini.</strong></a></p><h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/activated-resins/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Activated resins</strong></a></h4><p>Pre-activated resin enables successful, convenient immobilization of ligands without the need for complex syntheses or special equipment. We have developed two different pre-activated resins where the bromohydrin active group reacts with thiol, amino and hydroxyl groups of the substance to be coupled. Two different resin porosities are available to facilitate optimized coupling of ligands of different sizes, or to optimize the prepared affinity resin for target molecules of different sizes.</p><p><strong>Resin for coupling your own ligands:</strong></p><ul><li>Made from agarose, well established and well-known in the Biotechnology industry</li><li>Simple coupling procedures at ambient temperature</li><li>Stable at ambient temperature in aqueous solution and at neutral pH</li><li>Suitable for coupling of ligands containing sulphydryl, amino- or hydroxy group</li></ul><p>Available as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/activated-resins/activated-sorbents/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>WorkBeadsTM sorbents</strong></a><strong> </strong>or in pre-filled <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/bioprocessing-products/activated-resins/activated-columns/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>GoBio™ Mini</strong></a>.</p><p>For more information or a non-binding quote regarding BioWorks products, please contact us at <strong>watrex@watrex.com</strong> or visit our website <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>www.watrex.com</strong></a></p>

Waters Sample & Collection Plate

Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots.

<div><div><h4><strong>Broad selection of high-quality sample and collection plates</strong></h4></div></div><div><div><p><strong>Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots. Waters plates are made from polypropylene (PP) for chemical resistance, and specialty 96-well plates with glass inserts are available for samples that are not suitable for polypropylene containers.</strong></p><h3><strong>Overview</strong></h3><div><div><div><div><div><div><ul><li>96- and 384-well collection plates and sample plates are SBS/ANSI compliant for robotic compatible systems&nbsp; &nbsp; &nbsp; &nbsp;</li><li>&nbsp;Constructed of polypropylene (PP) for chemical resistance</li><li>&nbsp;Glass inserts available for samples requiring glass surfaces</li><li>&nbsp;Heat seals and cap mats for plates and glass inserts</li></ul></div></div><div><div><h4><strong>Recommended Use</strong></h4><p>For general high-throughput LC and LC-MS work and for integration in (semi)-automated sample handling workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/img_Waters_Sample_and_Collection_Plate_709693fdab.jpg" alt="imgWaters Sample &amp; Collection Plate." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_img_Waters_Sample_and_Collection_Plate_709693fdab.jpg 245w," sizes="100vw" width="1024" height="493"></p><h3><strong>Cap Mats</strong></h3><h4><strong>Silicone/PTFE &amp; Polypropylene Cap Mats</strong></h4><ul><li>Non-slit storage mats for long-term sample storage</li><li>Pre-slit silicone/PTFE and polypropylene cap mats for use in autosamplers for proper venting and sample draw accuracy with excellent resealing characteristics</li><li>Silicone/PTFE &amp; Polypropylene Cap Mats</li></ul><h3><strong>Heat Seals</strong></h3><h4><strong>Clear Polyester Heat Seals</strong></h4><ul><li>Can be used with most solvents &amp; buffers over a -80 °C to 80 °C temperature range</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Aluminum Foil Heat Seals</strong></h4><ul><li>Made from a polyester/aluminum laminate</li><li>Ideal choice for long term storage to reduce evaporative loss</li><li>Aluminum layer reduces gas permeability of the seal</li><li>Can be used at temperatures from -200 °C to 90 °C</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Adhesive Seals</strong></h4><ul><li>A polyolefin film with synthetic rubber adhesive</li><li>Ideal for use with protein and peptide samples and buffers</li><li>Resistant to small concentrations (0% to 30%) of polar organic solvents</li><li>Can be used at temperatures from -80 °C to 80 °C</li><li>Requires a heat sealer to apply</li></ul></div></div></div></div></div></div></div></div>

Waters CERTIFIED HPLC Vials

Reducing failed assays from unknown contaminant peaks and limiting instrument downtime from out of specification vials is a priority for your lab. Waters sample vials can help you do just that.

<div><div><h4><strong>Meet your analytical challenges with the right vial.</strong></h4></div></div><div><div><p><strong>The right vial.&nbsp;</strong><span><strong>A world of difference.</strong></span></p><p>Reducing failed assays from unknown contaminant peaks and limiting instrument downtime from out of specification vials is a priority for your lab. Waters sample vials can help you do just that.</p><p>All Waters sample vials are held to the tightest dimensional tolerances. Manufactured via a 2-, 3-, or 4-step certification process, we ensure that Waters Certified Vials meet and maintain specified vial dimensions, levels of cleanliness and adsorption properties from batch to batch.&nbsp;Find the right vial for your application and autosampler - use our <a target="_blank" rel="noopener noreferrer" href="https://find.waters.com/VialSelector/"><strong>Vial Selector</strong></a> to find your perfect match.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_118649175c.jpg" alt="Waters CERTIFIED Vials: Table 1. Vials for Routine and Advanced Analyses." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_118649175c.jpg 245w," sizes="100vw" width="1226" height="754"></p><h3><strong>Waters LCMS CERTIFIED</strong></h3><p><span><strong>✅ </strong></span>Dimensional Test</p><p><span><strong>✅ </strong></span>Septum GC Test</p><p><span><strong>✅ </strong></span>HPLC UV Test</p><p><span><strong>✅ </strong></span>MS Scan</p><ul><li>Low MS interferences from known residues &amp; polymers</li><li>For routine QC and LC-MS analysis with lower analyte concentrations from 10-100s ng/ml.</li></ul><h3><strong>Waters TruView<sup>TM</sup> LCMS CERTIFIED</strong></h3><p><span><strong>✅ </strong></span>Dimensional Test</p><p><span><strong>✅ </strong></span>Septum GC Test</p><p><span><strong>✅ </strong></span>HPLC UV Test</p><p><span><strong>✅ </strong></span>MS Scan</p><p><span><strong>✅&nbsp;</strong></span>Low Adsorption Test</p><div><div><ul><li>Lowest MS interferences from known residues &amp; polymers</li><li>For high-end LC-MS analysis and quantification of analytes with a concentration of below 1 ng/ml with reduced adsorption of polar analytes.</li></ul><h3><strong>Waters CERTIFIED</strong></h3><p><span><strong>✅ </strong></span>Dimensional Test</p><p><span><strong>✅ </strong></span>Septum GC Test</p><p><span><strong>✅ </strong></span>HPLC UV Test</p><div><div><ul><li>No UV interferences caused caused by manufacturing and packaging residues</li><li>For routine QC LC-UV analysis&nbsp;of compounds in µg/mL&nbsp;concentrations.&nbsp;</li></ul><h3><strong>MAXPEAK QuanRecovery</strong></h3><ul><li>For high recovery peptide and protein quantification workflows. Reduces sample losses due to non-specific binding (NSB) and ionic interactions.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_2_4fba532335.jpg" alt="Waters CERTIFIED Vials: Vials Shapes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_2_4fba532335.jpg 245w," sizes="100vw" width="1261" height="617"></p><div><div><h3><strong>Control data quality risks and reduce unexpected or ghost peaks</strong></h3><p>Don’t let the presence of background noise impact your results, causing data quality risks and unexpected or ghost peaks. Background noise can come from many factors.&nbsp;One of the most important factors are vial and septa cleanliness, which introduces contamination peaks. Ghost peaks are introduced by analyte degradation that can be triggered by significant pH changes in the diluent. In this chromatogram, the top shows normal behavior, and the bottom chromatogram shows ghost peaks.</p><p>Waters Certified vials are cleanliness tested so you don’t have to worry about contaminant peaks. The TruView pH Control Vials are certified to minimize and control a pH shift away from the neutral.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_4_7749301ba1.jpg" alt="Waters CERTIFIED Vials: Prevent the appearance of unexpected peaks during LC/UV analysis with Waters LC/GC Certified Vials as a control strategy." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_4_7749301ba1.jpg 245w," sizes="100vw" width="1024" height="535"></p><h3><strong>More time on development. Less on troubleshooting</strong></h3><p>Stop losing valuable development time on troubleshooting due to analyte loss from non-specific adsorption. Waters TruView pH Control LCMS Certified Vials have the lowest adsorptive losses on the market, and you can rely on them to provide consistency from batch to batch and vial to vial. This chromatogram shows a comparison of adsorptive loss of chlorhexidine in a variety of vials.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_5_d3e42fa7f2.jpg" alt="Waters CERTIFIED Vials: Comparison of adsorptive loss of chlorhexidine acetate in a variety of vials." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_5_d3e42fa7f2.jpg 245w," sizes="100vw" width="1024" height="633"></p><div><div><h3><strong>Ideal for demanding MS work</strong></h3><p>LCMS Certified Vials are suited for analyte concentrations in the 100s ng/mL concentration. Waters LCMS Certified Vials are tested by MS scans from 250 to 1000 <i>m/z</i> with specifications set on the total ion count and presence of clusters in the high mass range. This chromatogram shows a comparison of typical MS scans of Waters LCMS Certified Vials to vials of other sources and clean solvents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_3_4869fbf57a.jpg" alt="Waters CERTIFIED Vials: Comparing typical MS scans of Waters LCMS Certified Vials, with vials from other sources." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_3_4869fbf57a.jpg 245w," sizes="100vw" width="1024" height="406"></p><div><div><h3><strong>Stay in control of your samples' pH</strong></h3><p>The analysis of low abundance species, pH sensitive analytes or not yet well understood compound mixtures benefit from consistent and well controlled vial surfaces. The glass surface of TruView pH Control LCMS Certified Vials minimizes pH shifts away from the neutral and thus eliminates unwanted reactions and resulting ghost peaks.&nbsp;Ghost peaks are very difficult to troubleshoot.</p><p>Waters TruView pH Control LCMS Certified vials were used to analyze two compounds, drospirenone and lovastatin. The TruView pH Control LCMS Certified Vials show little to no degradant formed over the time tested. Standard glass vials showed considerable degradant formation over the same testing time with irreproducible formation of the degradant across different vials.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_6_4dc330d849.jpg" alt="Waters CERTIFIED Vials: UV peak areas of lovastatin degradant across replicate vials and different vial types. Error bars indicate standard deviation for the replicate injection peak area." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_6_4dc330d849.jpg 245w," sizes="100vw" width="1024" height="605"></p><div><div><h3><strong>Reducing adsorption starts here</strong></h3><p>Inconsistent analytical results from small molecule analyte adsorption onto the surface of glass vials can be a problem at today’s low analyte concentrations. Some scientists have even experienced total analyte loss with samples stored in vials for an extended time. The vial chemistry, specifically the&nbsp;glass surface, is the major contributor to this problem.</p><p>Polar analytes&nbsp;may adsorb to the glass vial surface by polar-polar interaction and/or ion exchange mechanisms, thereby reducing the overall amount of sample in solution. TruView Certified LCMS Vials&nbsp;reduce this problem dramatically due to a stringent manufacturing process. The process yields a glass surface that has a lower concentration of free ions on the surface.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_8_064003b933.jpg" alt="Waters CERTIFIED Vials: Nortriptyline at 1 ppb concentration in several vials. The tall peak is the initial injection; the smaller peak (overlaid) is the four-hour injection. The first two chromatograms are competitor’s vials. The third chromatogram is for the Waters TruView LCMS Certified vial. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_8_064003b933.jpg 245w," sizes="100vw" width="1912" height="1182"></p><div><div><h3><strong>No analyte left behind</strong></h3><p>Instrument development is yielding more sensitive mass spectrometers. With more sensitive instruments, analytes can be detected at pg/mL levels. At these concentrations, understanding adsorption issues is critical for good quantitative results. We tested adsorption loss for the major manufacturers and saw high sample loss across manufacturers and clear and amber glass types – with significantly less loss with Waters TruView Autosampler Vials.</p><h4><strong>Average Analyte Loss</strong></h4><p><strong>Manufacturer: Clear Glass / Amber Glass</strong></p><ul><li><strong>Vendor A</strong>: 55% / 69%</li><li><strong>Vendor B</strong>: 25% / 25%</li><li><strong>Vendor C</strong>: 54% / 74%</li><li><strong>Vendor D</strong>: 18% / 59%</li><li><strong>Vendor E</strong>: 75% / Not tested</li><li><strong>Waters TruView LCMS Certified Vial</strong>: 1% / 6%</li></ul></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters QuanRecovery HPS Vials & Plates

 QuanRecovery Vials and Plates minimize sample losses from NSB and ionic interactions, ensuring greater confidence in results and improved assay repeatability.

<div><div id="title-b8cf3c562d" data-locator="title-with-text"><h3><strong>Reduce sample losses due to non-specific binding (NSB)</strong></h3></div></div><div><div id="text-c1d5077d21" data-locator="text"><p><strong>Successfully quantitating proteins and peptides with LC–MS is often more challenging than doing so for small molecules. Their large and complex structure requires a different approach to LC–MS method development and may also cause other unique problems like nonspecific adsorption (or nonspecific binding). Proteins and peptides in solution tend to stick to the surfaces they encounter.</strong></p><p>Autosampler-ready QuanRecovery Vials and Plates with MaxPeak High Performance Surfaces (HPS)&nbsp;are designed to enable scientists like you to meet recovery, sensitivity and reproducibility challenges in LC-MS peptide and protein quantification. By reducing sample losses from non-specific binding (NSB) and ionic interactions, QuanRecovery Vials and Plates provide your lab greater confidence in analytical results and fewer long-term assay repeatability problems.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Quan_Recovery_HPS_Vials_and_Plates_Quan_Recovery_Vials_and_Plates_with_Max_Peak_HPS_ed65a9e3fe.jpg" alt="Waters QuanRecovery HPS Vials &amp; Plates: QuanRecovery Vials &amp; Plates with MaxPeak HPS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Quan_Recovery_HPS_Vials_and_Plates_Quan_Recovery_Vials_and_Plates_with_Max_Peak_HPS_ed65a9e3fe.jpg 234w," sizes="100vw" width="1024" height="682"></p><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-b5ef0a0cdd" data-locator="text"><div><div id="text-62116091dc" data-locator="text"><h3 data-locator="modal-title"><strong>Improved LC-MS analysis of peptides and proteins</strong></h3><p>It can be difficult to predict peptide losses due to non-specific binding and ionic interactions, as losses can be dependent on many characteristics, including pI and hydrophobicity. Demonstrated here are the recovery of four different peptides.&nbsp;The recovery of desmopressin is not affected by the sample container, while glucagon, insulin, and melittin suffer varying losses due to non-specific binding and ionic interactions in the glass and standard polypropylene containers.&nbsp;</p><p>Only QuanRecovery Vials and Plates with MaxPeak HPS protect these peptides from loss, enabling you to achieve higher recovery and improved repeatability.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Quan_Recovery_vialky_a_platicka_pro_lepsi_analyzy_proteinu_a_peptidu_caa551b7f1/Waters-QuanRecovery-vialky-a-platicka-pro-lepsi-analyzy-proteinu-a-peptidu_l.webp" alt="Waters QuanRecovery HPS Vials &amp; Plates: A comparison of the recovery of four peptides prepared in a typical LC-MS diluent and stored in various sample containers." width="1074" height="466"></p></div></div></div></div></div></div></div></div></div></div><div><div id="text-455dc5902f" data-locator="text"><h3 data-locator="modal-title"><strong>Enhanced recovery and sensitivity at high and low sample concentrations</strong></h3><p>Biomolecule sample losses are a problem at low concentrations. However, non-specific binding and ionic interactions can also cause losses at higher concentrations. Such losses not only negatively impact the assay sensitivity, they also compromise the reproducibility, precision, and accuracy of the analysis. QuanRecovery Vials and Plates with MaxPeak HPS protect your sample, ensuring improved analytical results.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Quan_Recovery_HPS_Vials_and_Plates_Improved_recovery_and_sensitivity_at_high_and_low_sample_concentrations_with_Quan_Recovery_Plates_2816689528.jpg" alt="Waters QuanRecovery HPS Vials &amp; Plates: Improved recovery and sensitivity at high and low sample concentrations with QuanRecovery Plates." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Quan_Recovery_HPS_Vials_and_Plates_Improved_recovery_and_sensitivity_at_high_and_low_sample_concentrations_with_Quan_Recovery_Plates_2816689528.jpg 221w," sizes="100vw" width="1024" height="724"></p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-9f8f97fb02" data-locator="text"><ul><li>For large molecule LC-MS bioanalysts who need the ultimate sensitivity, reproducibility and sample stability over multiple analyses.</li><li>Improve detection limits and analysis reproducibility for peptide and protein quantification with these low adsorption plates and vials.</li><li>Designed for LC-MS analysis in autosampler ready formats with low residual volumes in 700 µL 96-well plate and 300 µL sample vial formats.</li><li>Increase analyte recovery, sensitivity and reproducibility by minimizing analyte/surface interactions that can lead to sample losses with MaxPeak High Performance Surfaces (HPS).</li><li>Suitable for workflows with ACQUITY Premier Systems and Columns.</li></ul></div></div><div><div id="text-b5ef0a0cdd" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>To minimize sample losses due to non-specific binding (NSB) and ionic interactions.</p></div></div></div></div></div></div></div></div>

Waters Oasis SPE products

Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs.

<h4><strong>Faster, cleaner, and simpler SPE starts here</strong></h4><p>Analytical chemists worldwide use SPE to remove interfering matrix compounds and enrich the analyte of interest to ensure the generation of high-quality data.</p><p>Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.</p><p>Oasis SPE products deliver unmatched purity, consistency, and quality for sample analysis. Along with seven different SPE chemistries, Oasis SPE products use universal to customized protocols to handle your unique sample.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_2_967d461f2e/Waters-Oasis-SPE-kolonky-2.jpg" alt="Waters: Oasis SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_2_967d461f2e/thumbnail_Waters-Oasis-SPE-kolonky-2.jpg 245w," sizes="100vw" width="950" height="416"></p><h4><strong>Overview</strong></h4><ul><li>Fully water-wettable sorbents increase reproducibility and enable elimination of condition and equilibration steps</li><li>Co-polymer pH stable from 0–14 with no secondary silanol activity</li><li>Optimal sensitivity for small molecule and therapeutic peptide analysis</li><li>Reduced matrix effects for LC-MS/MS analyses</li><li>Unsurpassed selectivity</li><li>Consistent retention and capacity, yielding the highest SPE recovery</li><li>Reduces variability in analytical results and increases robustness of analysis</li><li><strong>Recommended Use: </strong>For the fastest, cleanest, and simplest approach to SPE-LC-MS/MS method development.</li></ul><h4><strong>Solid-Phase Extraction Chemistries</strong></h4><h5><strong>Oasis PRiME HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent for acids, bases, and neutrals</li><li>Cleaner samples in fewer steps with simple three-and two-step protocols, no SPE expertise required</li><li>Removes 95% of common matrix interferences, such as proteins, salts, phospholipids, and pigments</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Ideal for reversed-phase SPE clean-up of samples in routine analysis.</li></ul><h5><strong>Oasis PRiME MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent to selectively retain, concentrate and elute basic compounds</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Cleaner samples in fewer steps with simple and fast 3 and 4-step protocols</li><li>Removes 99% of phospholipids from complex sample matrices</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Highly reproducible target analyte recoveries</li><li>Ideal for basic compound specificity while removing matrix interferences in a simple clean-up.</li></ul><h5><strong>Oasis HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent</li><li>Provides high capacity for acids, bases, and neutrals</li><li>Ideal for ultra-high capacity of very polar compounds.</li></ul><h5><strong>Oasis MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent for bases</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of basic analytes.</li></ul><h5><strong>Oasis MAX</strong></h5><ul><li>Mixed-mode Anion eXchange for acids</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of acidic analytes.</li></ul><h5><strong>Oasis WAX</strong></h5><ul><li>Weak Anion eXchange mixed-mode sorbent for strong acidic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of strong acidic compounds.</li></ul><h5><strong>Oasis WCX</strong></h5><ul><li>Weak Cation eXchange mixed-mode sorbent for strong basic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of all basic analytes, especially strong bases.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png" alt="Waters: Oasis Sorbents" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/thumbnail_Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png 211w," sizes="100vw" width="1511" height="1119"></p><h4><strong>Oasis Formats</strong></h4><h5><strong>Oasis μElution Plate</strong></h5><ul><li>Patented μElution plate design</li><li>No evaporation and reconstitution necessary due to elution volumes as low as 25 µL</li><li>Ideal for SPE clean-up and analyte enrichment of small sample volumes</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg" alt="Waters: Oasis µElution Plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg 245w," sizes="100vw" width="502" height="260"></p><h5><strong>Oasis 96-Well Sample Extraction Plates</strong></h5><ul><li>Innovative, award-winning two-stage well design</li><li>High throughput and high recovery</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg" alt="Waters: Oasis 96-Well Sample Extraction Plates" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg 245w," sizes="100vw" width="525" height="329"></p><h5><strong>Oasis Syringe-Barrel Cartridges</strong></h5><ul><li>Ultra-clean syringe barrel and frits</li><li>Available in cartridges ranging from 1 cc to 60 cc</li><li>Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes</li><li>Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg" alt="Waters Corporation: Oasis Syringe-Barrel Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg 245w," sizes="100vw" width="503" height="311"></p><h5><strong>Oasis Glass Cartridges</strong></h5><ul><li>Ultra-clean glass syringe with Teflon® frit</li><li>For trace level detection and analysis at part-per-trillion (PPT) levels</li><li>Available in 5 cc (200 mg) configuration</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg" alt="Waters: Oasis Glass Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg 188w," sizes="100vw" width="409" height="339"></p><h5><strong>Oasis On-Line Columns and Cartridges</strong></h5><ul><li>For rugged, reproducible, and ultra-fast on-line analysis</li><li>Wide choice of configurations, particle sizes, and sorbent chemistries</li><li>Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX</li><li>High recovery and reproducible results for a wide range of compounds</li><li>Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg" alt="Waters: Oasis On-Line Columns and Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg 168w," sizes="100vw" width="415" height="386"></p><h4><strong>Sample preparation, improved</strong></h4><p>Improving various aspects of chromatography and mass spectrometry analyses often starts with sample preparation. &nbsp;Solid-phase extraction (SPE) or "liquid-solid phase extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing matrix components in a sample. &nbsp;</p><p>SPE is a proven sample preparation tool for simple sample matrix removal, including common interferences like proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including matrix effect reduction and an increase in sample concentration and analyte specificity. Matrix removal also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC and reducing signal suppression by providing clean samples for the MS.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg" alt="Waters: Oasis SPE - LC-MS-MS chromatogram of 1 pgmL of alprazolam from rat plasma" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg 169w," sizes="100vw" width="1024" height="946"></p><h4><strong>Get the cleanest extracts with the Oasis 2x4 method</strong></h4><p>Having a variety of tools for method development allows separation scientists to optimize protocols for maximum sensitivity and selectivity. The Oasis 2x4 method for SPE sorbent and protocol selection for acids, bases, and neutrals produces the cleanest extracts – all by eliminating matrix interferences and sample variability. Get the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis with our two protocols and four sorbents. By following the simple steps outlined in the Oasis 2x4 method, you can achieve high recoveries and the cleanest extracts.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg" alt="Waters: Oasis SPE - Oasis 2 x 4 method development protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/thumbnail_Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg 242w," sizes="100vw" width="1519" height="979"></p><h4><strong>Isolate your compounds with the Oasis HLB method</strong></h4><p>Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg" alt="Waters: Oasis SPE - Generic Method Protocol with Oasis HLB SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg 139w," sizes="100vw" width="1024" height="1153"></p><h4><strong>Multiple contaminant analysis with the 2-step pass-through protocol</strong></h4><p>If you are doing multiple residue screening of veterinary drugs, pesticides and mycotoxins in various food matrices, you may be facing a high concentration of organic solvents. With our simple 2-step protocol (pass-through SPE), you can remove matrix interferences quickly if your samples contain a high concentration of organic solvent(s) and concentration and/or salt removal is not required. It can be used as the clean-up method following QuEChERS extraction, saving you significant time compared to the traditional dispersive SPE method - all while achieving superior clean-up.</p><p><strong>To do pass-through SPE, Waters offers 2 convenient and flexible formats:</strong></p><ul><li><strong>Traditional-style cartridges</strong> compatible with vacuum and positive pressure manifolds&nbsp;</li><li><strong>"Plus" style cartridges</strong> that can be used without manifolds when connected to a displacement syringe</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_3_b4da6242de/Waters-Oasis-SPE-kolonky-3.jpg" alt="Waters: Oasis Plus Light and Plus Short Cartridges for 2-step pass through protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_3_b4da6242de/thumbnail_Waters-Oasis-SPE-kolonky-3.jpg 152w," sizes="100vw" width="1308" height="1342"></p>

Thermo Scientific QuEChERS Sample Preparation Kits

QuEChERS is a versatile sample preparation method for analyzing pesticides, veterinary drugs, and other residues in various sample types before GC-MS or LC-MS analysis.

<div><div><div><h3><strong>QuEChERS sample preparation—now easier, more convenient, and affordable</strong></h3><p><strong>QuEChERS</strong> (<strong><u>Q</u></strong><u>u</u>ick, <strong><u>E</u></strong>asy, <strong><u>C</u></strong>heap, <strong><u>E</u></strong>ffective, <strong><u>R</u></strong>ugged, and <strong><u>S</u></strong>afe)is a <strong>sample preparation method</strong> that was initially developed in 2003 for <strong>analyzing veterinary drugs in food</strong>. The method was also tested for <strong>pesticide residue analysis in plant material</strong> with great success. In 2003, the <strong>detailed method was published</strong> in the journal AOAC International. Since then, the QuEChERS method has evolved into a <strong>sample preparation technique used on a variety of analytes</strong> in an <strong>expanding number of sample types</strong> before analysis by <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/gas-chromatography-mass-spectrometry-gc-ms.html"><strong>GC-MS</strong></a><strong> or </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms.html"><strong>LC-MS</strong></a>.</p><p><strong>Thermo Scientific QuEChERS kits</strong> contain <strong>pre-packaged, ready-weighed salts, sorbents, and buffers</strong> to <strong>streamline your workflow</strong> and <strong>minimize the potential for error</strong>. The kits are grouped according to your <strong>analytes of interest</strong>, <strong>sample matrix</strong>, and <strong>preferred QuEChERS method</strong>:</p><ul><li><strong>Original 2003 Method</strong></li><li><strong>AOAC 2007.01 Method</strong></li><li><strong>EN 15662 Method</strong></li><li><strong>NY/T 1380-2007 Method</strong></li></ul><p><strong>Complete packages</strong>—including both <strong>extraction and clean-up kits</strong>—are available. <strong>Extraction-only and cleanup-only kits</strong> are also offered to suit your laboratory’s sample preparation needs. These <strong>pre-packaged kits save time and money</strong>, make QuEChERS procedures <strong>easier and more convenient</strong>, and ensure <strong>quality to yield reproducible results</strong> and <strong>excellent recoveries</strong> for a wide variety of analytes.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_2_1e99f0c81b.jpg 217w," sizes="100vw" width="891" height="640"></p><h3><strong>Typical QuEChERS workflow</strong></h3><div><div><div><div><div><p>As shown in the workflow diagram below, the <strong>QuEChERS method has four basic steps</strong>:</p><ol><li><strong>Homogenize sample</strong> (fruit or vegetable)</li><li><strong>Extract analytes of interest</strong> from a sample using <strong>extraction salts</strong> (pesticide residues, veterinary drugs, and/or mycotoxins)</li><li><strong>Clean-up the resulting supernatant</strong> using <strong>dispersive solid phase extraction (dSPE)</strong></li><li><strong>Analyze by GC-MS or LC-MS</strong></li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_496491ceff.jpeg 245w," sizes="100vw" width="1170" height="706"></p><div><h3><strong>QuEChERS products</strong></h3></div><div><h4><strong>QuEChERS extraction kits for original 2003 method</strong></h4></div><div><p>The <strong>original QuEChERS method</strong> (Anastassiades et al, 2003) was developed by <strong>Steven J. Lehotay and Michelangelo Anastassiades</strong> in 2003 for the <strong>determination of pesticide residues in fruits and vegetables</strong>. The original method was created to <strong>analyze non-base-sensitive compounds</strong> and uses a <strong>sodium chloride buffer</strong> to <strong>reduce polar interferences</strong> and <strong>enhance extraction</strong>. <strong>Buffering</strong> is an important part of the QuEChERS procedure because it <strong>allows for analysis of pesticides and other residues</strong> that display <strong>pH dependence in their recoveries</strong>, depending on the <strong>sample matrix</strong>. <strong>Three different extraction kits</strong> are available to support the <strong>original 2003 QuEChERS method</strong>.</p><h5><strong>Chemicals used in the original 2003 method</strong></h5></div><ul><li><strong>Acetonitrile:</strong> Used to perform single-phase extraction (SPE) on a sample</li><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>NaCl:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample; reduces polar interference and enhances extraction</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li></ul><div><h4><strong>QuEChERS extraction and clean-up kits for AOAC 2007.01 method</strong></h4><p>The <strong>AOAC 2007.01 QuEChERS Method</strong> was developed by <strong>Steven J. Lehotay and his co-workers</strong>, who <strong>modified the original 2003 method</strong> to accommodate the use of <strong>acetate buffering at higher concentration</strong> for <strong>greater buffering strength</strong>. <strong>Buffering</strong> is an important part of the QuEChERS procedure because it <strong>allows for analysis of pesticides and other residues</strong> that display <strong>pH dependence in their recoveries</strong>, depending on the <strong>sample matrix</strong>. These <strong>extraction and clean-up products</strong> here support the <strong>AOAC 2007.01 method</strong>.</p><div><h5><strong>Chemicals used in the AOAC 2007.01 method</strong></h5><ul><li><strong>Acetonitrile:</strong> Used to perform single-phase extraction on a sample</li><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>NaOAc:</strong> Buffers the sample to stabilize pH</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>C18:</strong> Removes fats, sterols, and other non-polar interferences from sample</li><li><strong>GCB:</strong> Graphitized carbon black, removes pigment (<i>not recommended for use with planar pesticides</i>)</li></ul><div><h4><strong>QuEChERS extraction and clean-up kits for EN 15662 method</strong></h4><p>The <strong>extraction and clean-up products</strong> here support the <strong>EN 15662 method</strong>. <strong>Widely used in Europe</strong>, the EN 15662 method was developed by <strong>Michelangelo Anastassiades and co-workers</strong>, who <strong>modified the original 2003 method</strong> to accommodate the use of <strong>citrate buffering at a relatively low buffering capacity</strong>. This variation uses a <strong>sodium chloride buffer</strong> to <strong>minimize polar interferences</strong> in addition to <strong>sodium citrate dihydrate</strong> and <strong>disodium citrate sesquihydrate buffers</strong>. <strong>Buffering</strong> is an important part of the QuEChERS procedure because it <strong>allows for analysis of pesticides and other residues</strong> that display <strong>pH dependence in their recoveries</strong>, depending on the <strong>sample matrix</strong>.</p><h5><strong>Chemicals used in the EN 15662 method</strong></h5><ul><li><strong>Acetonitrile:</strong> Used to perform single-phase extraction on a sample</li><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>NaCl:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes residual water from sample</li><li><strong>Trisodium Citrate:</strong> Buffers the sample to stabilize pH</li><li><strong>Disodium Citrate Sesquihydrate:</strong> Buffers the sample to stabilize pH</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>C18:</strong> Removes fats, sterols, and other non-polar interferences from sample</li><li><strong>GCB:</strong> Graphitized carbon black, removes pigment (<i>not recommended for use with planar pesticides</i>)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg" alt="Thermo Scientific QuEChERS Sample Preparation Kits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Qu_E_Ch_ERS_Sample_Preparation_Kits_3_721562526f.jpg 245w," sizes="100vw" width="1471" height="488"></p><div><h4><strong>QuEChERS clean-up kit for NY/T 1380-2007 method</strong></h4></div><div><p>The <strong>NY/T 1380-2007 method</strong> is <strong>recommended by China Ministry of Agriculture</strong> for the <strong>QuEChERS Method sample preparation</strong> of <strong>51 specific pesticide residues</strong> in <strong>fruits and vegetables</strong> with <strong>analysis by GC-MS</strong>.</p><h5><strong>Chemicals used in the NY/T 1380-2007 method</strong></h5><ul><li><strong>Anhydrous MgSO₄:</strong> Induces phase separation between water content in sample and acetonitrile layer during liquid-liquid partitioning step; removes remaining water from sample during dispersive solid phase extraction (dSPE) step</li><li><strong>PSA:</strong> Primary secondary amine, used to remove polar matrix components, citric co-extractives, and free fatty acids, from sample during dSPE step</li><li><strong>C18:</strong> Removes fats, sterols, and other non-polar interferences from sample</li></ul></div></div></div></div></div></div></div></div></div></div></div></div>

Products from category Consumables | LabRulez LCMS

Related content

EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape

Am I Paying Too Much for my Nitrogen Gas Cylinders?

Are you scientifically prepared for the new mushroom-picking season?