Phenomenex Strata X-CW Solid Phase Extraction (SPE)

Weak Cation Mixed-Mode Solid Phase Extraction (SPE)

A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.

Phenomenex: Strata-X-CW SPE

• Recommended Use: Extraction of basic compounds, particularly strong bases

Starting Method

For Bases with pKa > 8

• Column: 1 mL Methanol
• Equilibrate: 1 mL Water, pH 6-7
• Load: Diluted Sample, pH 6-7
• Wash 1: 1 mL Water, pH 6-7
• Wash 2: 1 mL Methanol (collect this fraction to analyze Neutrals/Acids)
• Elute Any Bases: 2x 500 µL 5 % Formic Acid in Methanol
• Elute Weak Bases: 2x 500 µL 5 % NH4OH in Methanol

Phenomenex: Strata X single tube

Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Available Formats

• 96-Well-Plate

Phenomenex: Strata X 96-well plate

• 1, 3, and 6mL Tubes

Phenomenex: Strata X 1, 3, and 6mL Tubes

• Giga Tubes (12-150mL Tubes)

Phenomenex: Strata X Giga Tubes (12-150mL Tubes)