Phenomenex Biozen N-Glycan Clean-Up

Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)

SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.

• Small volume format allows for more concentrated samples
• Minimal elution solvent
• Excellent retention and recovery of labeled, released N-glycans
• Faster clean-up methods to save on sample prep time

Protocol

• 96-Well Plate: Biozen N-Glycan Clean-up
• Part No.: 8M-S009-NGA
• Condition: 200 µL Water
• Equilibrate: 200 µL Water/Acetonitrile (15:85)
• Load: Labeled Sample (up to 400 µL of labeled sample)
• Wash: 2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
• Elute: 3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
• Dilute: 100 µL Dimethylformamide and then 210 µL of Acetonitrile

Phenomenex: Biozen N-Glycan Clean-up