<h4><strong>2nd Annual International NETPHARM Conference: From Cutting-Edge Equipment to Excellent Results at the Midpoint of the Project</strong></h4><p><strong>On 15–16 April 2026, the second annual international conference of the project “New Technologies for Translational Research in Pharmaceutical Sciences” (NETPHARM) took place in Kudowa-Zdrój, Poland. The meeting, attended by more than 100 researchers, confirmed that after two years of implementation the project is successfully fulfilling its ambitious goals in linking basic research with clinical practice.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mgr_Martina_Kopecka_Jurcekova_Prof_Petr_Pavek_8db8f7e5f2.jpg" alt="Mgr. Martina Kopecká Jurčeková: Prof. Petr Pávek" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mgr_Martina_Kopecka_Jurcekova_Prof_Petr_Pavek_8db8f7e5f2.jpg 234w," sizes="100vw" width="1200" height="800"></p><h4><strong>Scientific excellence and international outreach</strong></h4><p>The scientific programme of the conference focused on progress in five key research objectives (RO1–RO5), with the leaders of individual sections presenting achieved milestones, scientific results, and team synergies over the past year.<strong> Prof. Kateřina Vávrová</strong> presented on pharmaceutical and analytical chemistry (RO1), while progress in pharmacology and toxicology (RO2) was summarized by <strong>Prof. František Štaud</strong>. <strong>Prof. Martin Hrubý</strong> presented results in the field of novel drug delivery systems and formulations (RO3), and <strong>Assoc. Prof. Daniela Fialová</strong> (RO4) introduced research in geriatric clinical pharmacy focused on systematic literature reviews, algorithms for individualized pharmacotherapy, and pilot deprescribing interventions. Evaluation of activities in advanced in vitro and in silico approaches in drug development (RO5) was presented by <strong>Assoc. Prof. Tomáš Smutný.</strong> Among the keynote speakers were, for example, <strong>Prof. Martin Hrubý</strong> with a lecture on stimuli-responsive polymers for biomedical applications, and <strong>Prof. František Švec</strong>, who addressed the use of monoliths in supercritical fluid chromatography for the separation of large biologically active molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mgr_Martina_Kopecka_Jurcekova_2_vyrocni_mezinarodni_konference_NETPHARM_prof_Frantisek_Svec_0b7cb8a321.jpg" alt="Mgr. Martina Kopecká Jurčeková: Prof. František Švec" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mgr_Martina_Kopecka_Jurcekova_2_vyrocni_mezinarodni_konference_NETPHARM_prof_Frantisek_Svec_0b7cb8a321.jpg 234w," sizes="100vw" width="1200" height="800"></p><p>A key contribution was the participation of members of the <strong>International Scientific Advisory Board (ISAB)</strong>, led by <strong>Prof. Maria da Conceição</strong> from the University of Porto. As part of invited lectures, these distinguished experts presented the latest global trends: <strong>Prof. Kevin A. Schug </strong>(<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/301"><strong>University of Texas at Arlington</strong></a>) focused on the characterization of advanced drug formulations, <strong>Prof. Igor Lacík </strong>(Slovak Academy of Sciences) discussed the use of polymers in diabetes treatment, <strong>Prof. Søren Bak</strong> (University of Copenhagen) presented plant natural products as next-generation biopesticides, and <strong>Prof. Michael Lämmerhofer</strong> (University of Tübingen) analyzed the importance of metabolomics and lipidomics in biomedical research.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mgr_Martina_Kopecka_Jurcekova_prof_Kevin_A_Schug_b31db2c431.jpg" alt="Mgr. Martina Kopecká Jurčeková: Prof. Kevin A. Schug" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mgr_Martina_Kopecka_Jurcekova_prof_Kevin_A_Schug_b31db2c431.jpg 234w," sizes="100vw" width="1200" height="800"></p><h4><strong>Key results: 110 publications and investment in infrastructure</strong></h4><p>The NETPHARM project, funded by the Jan Amos Komenský Operational Programme (OP JAK), achieved significant milestones by early 2026:</p><ul><li><strong>Interdisciplinary expert team: </strong>More than 200 researchers, both experienced and early-career scientists, have been involved in research activities within the project.</li><li><strong>Publications:</strong> <strong>110 scientific publications</strong> have been produced, the majority of which fall into the Q1 category.</li><li><strong>Dissemination and outreach:</strong> The number of contributions at international conferences (195) already exceeds the original plan by 38%.</li><li><strong>Infrastructure: 16 state-of-the-art instruments</strong> have been acquired for research laboratories, with a total value of CZK 37.5 million, including an X-ray diffractometer, confocal microscopy, and a computational server.</li><li><strong>Audit: </strong>An on-site inspection by the Ministry of Education, Youth and Sports was successfully completed, confirming the efficient use of grant funds.</li></ul><h4><strong>Strategic recommendations for the future</strong></h4><p>The conference was followed on 17 April by the <strong>3rd</strong> <strong>meeting of the ISAB,</strong> held in the historic premises of the Carolinum in Prague. The ISAB appreciated the dynamic collaboration among institutions such as <strong>Charles University (Faculty of Pharmacy and Faculty of Medicine in Hradec Králové), the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/11"><strong>University of Chemistry and Technology in Prague</strong></a><strong>, institutes of the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/10"><strong>Czech Academy of Sciences</strong></a><strong> (</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/62"><strong>IOCB Prague</strong></a><strong> and IMC), and </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/12"><strong>Masaryk University</strong></a><strong>/</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"><strong>CEITEC</strong></a>. For the remaining half of the project, experts recommended an even stronger focus on international mobility of project teams, strengthening synergies among individual teams, accelerating the patent application process, and focusing on the soft skills of junior researchers.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mgr_Martina_Kopecka_Jurcekova_3_zasedani_rady_ISAB_0121b9b78d.jpg" alt="Mgr. Martina Kopecká Jurčeková: 3rd meeting of the ISAB" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mgr_Martina_Kopecka_Jurcekova_3_zasedani_rady_ISAB_0121b9b78d.jpg 208w," sizes="100vw" width="1200" height="900"></p><h4><strong>Networking and support for talent</strong></h4><p>In addition to the intensive scientific programme, there was also a social programme, including a tour of Kudowa-Zdrój and a joint dinner. An important part was the award for the best poster presentations by young scientists, highlighting the project’s emphasis on educating a new generation of pharmaceutical experts. Among the awardees were <strong>Ing. Anna Tvrdá, Mgr. Mária Bajnoková, and Mary Angelia Alfred, M.Sc.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mgr_Martina_Kopecka_Jurcekova_oceneni_nejlepsich_posterovych_prezentaci_37ba838bed.jpg" alt="Mgr. Martina Kopecká Jurčeková: Award for the best poster presentations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mgr_Martina_Kopecka_Jurcekova_oceneni_nejlepsich_posterovych_prezentaci_37ba838bed.jpg 234w," sizes="100vw" width="1200" height="800"></p><blockquote><p>“<i>NETPHARM is at the midpoint of its journey and clearly demonstrates that building a nationwide platform of excellence makes sense. Our scientific outputs and modernized facilities form a strong foundation for innovations that will directly influence the quality of pharmaceutical care in the future</i>,” said <strong>Prof. Petr Pávek</strong>, the project’s principal investigator, summarising the meeting.</p></blockquote>

2nd Annual International NETPHARM Conference

<p><strong>High-performance liquid chromatography (HPLC) remains one of the most widely used analytical techniques for separating, identifying, and quantifying compounds in complex mixtures. Successful HPLC method development depends not only on selecting the correct column and optimizing the mobile phase composition, but also on choosing the appropriate detector. Even a perfectly optimized chromatographic separation provides little value if the detection system cannot properly capture and interpret the analytes of interest.</strong></p><p>In many ways, the detector acts as the <i><strong>“vision system</strong></i>” of the chromatograph. It converts separated compounds into measurable electronic signals, allowing analysts to identify components and determine their concentrations. The choice of detector therefore has a major impact on sensitivity, selectivity, robustness, and the overall quality of analytical data.</p><p>This article provides an overview of the most common HPLC detection techniques, discusses their strengths and limitations, and outlines the most important criteria to consider when selecting a detector for a specific analytical task.</p><h3><strong>HPLC Detection Technologies</strong></h3><p>HPLC detectors are typically divided into two major categories:</p><ol><li>Specific detectors (solute-property detectors)</li><li>Bulk property detectors</li></ol><p>Specific detectors respond to a particular chemical or physical property of the analyte. Ideally, their signal is largely unaffected by changes in mobile phase composition. Common examples include UV/Vis detectors, fluorescence detectors, and mass spectrometers.</p><p>Bulk property detectors, on the other hand, measure differences between the pure mobile phase and the mobile phase containing analytes. These detectors are considered more universal because they respond to properties shared by many compounds. Refractive index detectors are among the best-known examples, alongside conductivity and light-scattering detectors.</p><p>Both detector categories have important advantages depending on the application, sample type, and analytical goals.</p><h3><strong>Common Detector Types Used in HPLC</strong></h3><p>A wide variety of detectors are available for HPLC systems, ranging from highly universal devices to extremely selective and sensitive technologies.</p><p>Common detector options include:</p><ul><li>UV/Vis detectors</li><li>Diode array detectors (DAD/PDA)</li><li>Fluorescence detectors</li><li>Refractive index detectors (RID)</li><li>Conductivity detectors</li><li>Evaporative light scattering detectors (ELSD)</li><li>Charged aerosol detectors (CAD)</li><li>Mass spectrometry detectors (MS)</li></ul><p>Each technology offers a different balance of sensitivity, selectivity, robustness, operational complexity, and cost.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg" alt="KNAUER: Detector overview with characteristics. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Detector_overview_with_characteristics_Graphic_by_KNAUER_84b0dd30b1.jpg 245w," sizes="100vw" width="1293" height="596"></p><h3><strong>Selecting the Right HPLC Detector</strong></h3><p>There is no universally ideal detector for all HPLC applications. The best choice depends on several factors, including analyte chemistry, matrix complexity, required detection limits, and instrument compatibility.</p><p>Careful detector selection is essential for obtaining reliable and meaningful analytical results.</p><p>The following considerations can help guide the selection process.</p><h4><strong>1. Chemical and Physical Properties of the Analytes</strong></h4><p>The chemical nature of the compounds being analyzed is one of the most important selection criteria.</p><p><strong>Questions to consider include:</strong></p><ul><li>Do the analytes contain chromophores?</li><li>Are the compounds naturally fluorescent?</li><li>What is their polarity?</li><li>What is their molecular weight?</li><li>Are they volatile or non-volatile?</li></ul><p>Compounds containing UV-active groups are often well suited for UV/Vis detection, while fluorescent molecules can benefit from the high sensitivity of fluorescence detectors. Non-UV-active compounds may require universal detection approaches such as CAD, ELSD, or mass spectrometry.</p><h4><strong>2. Required Sensitivity</strong></h4><p>Detector sensitivity determines the minimum concentration that can be reliably detected and quantified.</p><p>Routine quality control analyses often rely on UV/Vis detectors because they provide sufficient sensitivity for many applications while remaining relatively simple and cost-effective. However, trace-level analysis or highly complex matrices may require more advanced technologies such as:</p><ul><li>Fluorescence detection</li><li>Charged aerosol detection</li><li>Mass spectrometry</li></ul><p>These techniques offer significantly lower detection limits and greater analytical specificity.</p><h4><strong>3. Detector Linearity</strong></h4><p>Linearity describes how well detector response correlates with analyte concentration across a defined range.</p><p>A detector with strong linearity ensures accurate quantification and simplifies calibration procedures. Poor linearity can introduce quantification errors, especially when analyzing samples across broad concentration ranges.</p><h4><strong>4. Dynamic Range</strong></h4><p>Dynamic range refers to the concentration interval over which the detector can reliably measure analytes.</p><p>Applications involving samples with highly variable concentrations require detectors capable of maintaining accurate response over a broad range. A wide dynamic range reduces the need for repeated dilutions or multiple analytical runs.</p><h4><strong>5. Selectivity</strong></h4><p>Selectivity defines the detector’s ability to distinguish target analytes from other matrix components.</p><p>This factor becomes particularly important for complex samples containing many interfering substances. Highly selective detectors reduce the risk of overlapping signals and false positives.</p><p>Mass spectrometry is often advantageous when dealing with unknown compounds or highly complicated sample matrices because it provides structural information in addition to quantitative data.</p><h4><strong>6. Budget Considerations</strong></h4><p>Detector cost is another practical factor that laboratories must evaluate.</p><p>UV/Vis and refractive index detectors are generally among the more economical choices, making them attractive for routine analyses and standard applications.</p><p>More sophisticated technologies such as fluorescence detection, CAD, or mass spectrometry typically require:</p><ul><li>Higher initial investment</li><li>Additional maintenance</li><li>More complex infrastructure</li><li>Greater operational expertise</li></ul><p>However, the overall value of the detector should always be considered in relation to analytical performance requirements and long-term application needs.</p><h4><strong>7. Instrument and Mobile Phase Compatibility</strong></h4><p>The selected detector must be compatible with the operational conditions of the HPLC system.</p><p>Important parameters include:</p><ul><li>Flow rate range</li><li>Pressure limitations</li><li>Mobile phase composition</li><li>Solvent compatibility</li></ul><p>Some detectors are highly sensitive to mobile phase composition or gradient changes, while others tolerate a broader range of operating conditions.</p><p>Ensuring proper compatibility helps prevent baseline instability, signal interference, and reduced detector performance.</p><h4><strong>8. Destructive vs. Non-Destructive Detection</strong></h4><p>Another important consideration is whether the analytical workflow involves only detection or also preparative purification.</p><p>Non-destructive detectors allow collected fractions to remain intact after detection, which is highly beneficial for preparative chromatography and purification workflows.</p><p>Destructive detectors, by contrast, consume or alter the sample during measurement and are therefore more suitable for analytical applications where sample recovery is not required.</p><h4><strong>9. Reliability and Ease of Operation</strong></h4><p>Practical usability is often just as important as analytical performance.</p><p>Some detectors are designed for straightforward plug-and-play operation, while others require:</p><ul><li>Frequent calibration</li><li>Specialized maintenance</li><li>Dedicated gas supplies</li><li>Vacuum systems</li><li>Advanced operator expertise</li></ul><p>Laboratories should evaluate the available technical support, user experience, and maintenance capabilities before implementing more sophisticated detection technologies.</p><p>Reliable operation and manageable maintenance requirements can significantly improve laboratory productivity and reduce downtime.</p><p>Choosing the correct HPLC detector starts with understanding both the sample and the analytical objective. Rather than selecting a detector simply because it is available, laboratories should ensure that the technology aligns with the chemistry of the analytes, required performance characteristics, and workflow demands.</p><p>In many cases, combining multiple detectors within a single HPLC system can provide even greater analytical insight by delivering complementary information from the same chromatographic separation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg" alt="KNAUER: Flow scheme for detector selection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Flow_scheme_for_detector_selection_Graphic_by_KNAUER_486004045f.jpg 245w," sizes="100vw" width="1316" height="808"></p><p>Interested in learning more? Future articles will explore individual HPLC detection techniques in greater detail, including their operating principles, application areas, and practical advantages for different analytical workflows.</p>

A comprehensive guide for selecting the appropriate HPLC detector

News from LabRulezLCMS Library - Week 20, 2026

Article

Product

Application

Academy

Science and research

The Faculty of Pharmacy, with its seat in the town of Hradec Králové, was established by a government decree in 1969. This Faculty of Pharmacy has continued in the old and long-time tradition of the education of pharmacy at Charles University.

Faculty of Pharmacy, Charles University

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

KNAUER LH 8.1 - Modular analytical XYZ Autosampler

KNAUER Liquid Handler offers finally an alternative in the world of robotic autosamplers. In-house design and development for a new level of simplicity and speed.

<p><strong>KNAUER Liquid Handler offers finally an alternative in the world of robotic autosamplers. In-house design and development for a new level of simplicity and speed, namely by:</strong></p><h4><strong>Performance</strong></h4><ul><li>Highest repeatability of peak area in full loop or sandwich injection mode</li><li>Zero sample loss and high precision via Sandwich injection mode&nbsp;</li><li>Lowest carry over with active Fast wash station that cleans the injection syringe and needle on the in- and outside by delivering an adjustable cleaning solvent flow</li><li>User-friendly control via pre programmed injection parameters in Chromatography Software packages</li></ul><h4><strong>Versatility</strong></h4><ul><li>Modular design and wide selection of accessories</li><li>Flexible injection volumes by a variety of exchangeable injection syringes&nbsp;</li><li>Customizable lengths of the rail from standard &nbsp;557 or 887 mm up to 1200mm to increase the amount of modules</li></ul><h4><strong>High throughput</strong></h4><ul><li>Up to 390 x 1.5 mL vials or 6 well plates per Robotic Cooler</li><li>Maximum 1560 x 1.5 ml vials or 24 well plates per Liquid Handler</li><li>Short cycle times via fast and precise movements and the software option "overlapped injections" that allows for preparing the next injection while the previous is still running</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_AZURA_system1_7ad2b43571.jpg" alt="Knauer: Liquid Handler LH 8.1 with AZURA system" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_AZURA_system1_7ad2b43571.jpg 244w," sizes="100vw" width="1390" height="889"></p><h3><strong>Great variety of modules available</strong></h3><p><strong>KNAUER offers a range of active and passive modules for easy customisation of your LH 8.1.</strong></p><ul><li>Want to have more samples and need a cooling option? Use the Robotic Cooler!</li><li>Want a lower carry over? Install the Fast Wash Station with two different solvents for in- and outside syringe and needle cleaning!</li><li>Want to change the solvent in your syringe? Use a passive solvent option to draw liquid during the injection process!</li></ul><p>There are a variety of active and passive options available. Just mount the module on the sampler, configure it in your software and you are ready to go.</p><h4><strong>Injection Valve</strong></h4><p>The injection valve consists of a valve drive and a 6 Port 2-Position valve with a special injection port.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_standalone1_5f21514cca.jpg" alt="Knauer: Liquid Handler LH 8.1 - standalone" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_standalone1_5f21514cca.jpg 179w," sizes="100vw" width="1293" height="1127"></p><h4><strong>Tray Holder</strong></h4><p>The manual tray holder can store up to 130 standard HPLC vials and is recommended whenever no sample cooling is needed.</p><h4><strong>Robotic Cooler</strong></h4><p>The Robotic Cooler can store your samples at 4 - 40°C. You can insert up to 3 Sample Racks for 130 vials each, what means you can store up to 390 sample vials per Robotic Cooler.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_cooled_tray_2_c6fd0672e9.jpg" alt="Knauer: Liquid Handler LH 8.1 - cooled tray" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_cooled_tray_2_c6fd0672e9.jpg 239w," sizes="100vw" width="1595" height="1043"></p><h4><strong>Fast Wash Station</strong></h4><p>With the fast wash station, the injection syringe is cleaned on the in- and outside. It is always recommended, because it significantly reduces carry-over and offers a very user-friendly handling.&nbsp;</p><h4><strong>Manual Wash Station</strong></h4><p>Manual wash stations are an alternative to the fast wash station when carry-over is not the major concern. A manual wash station can be integrated in the manual tray holder with 5 x 10 ml vials or used as an additional module with 6 x 10 ml vials or 2 x 60 ml vials.</p><h4><strong>Sample Trays</strong></h4><p>Different sample trays can be used either in the manual rack holder or in the Robotic Cooler. Depending on your needs, they are made for analytical standard 1.5 ml vials or well plates.</p><h4><strong>Syringes</strong></h4><p>The most common syringes are implemented in the chromatography software packages. All optimized parameters are preconfigured to get quickly started.</p><h3><strong>Special features</strong></h3><h4><strong>Injection modes</strong></h4><h5><strong>Sandwich loop: Zero sample loss with excellent reproducibility</strong></h5><p><strong>Tired of choosing between sample loss and high performance? The new injection mode offers both.</strong></p><ul><li>KNAUER Liquid Handler LH 8.1 has a special injection mode. Next to standard injections like full loop and partial loop it has the opportunity to use the sandwich injection mode.</li><li>The sample is prepared inside a gastight syringe and then transferred into the HPLC System. In sandwich mode the sample is trapped between small gaps of air. This injection method only takes up the sample volume it needs and the air gaps prevent any peak broadening. Furthermore the pressure increase during the injection process will reduce the sample plug even more leading ot much narrower peak shapes and higher injection precision. &nbsp;</li><li>Ths injection method combines the zero sample loss of µl pickup and injection precision of partial loop injection.</li><li>Only the amount of sample that is injected is aspired by the syringe</li><li>Minimized sample widenening during injection process</li><li>Very high precision</li><li>No loss of sample</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Sandwich_Injection_Mode_Big_fb713e65a0.jpg" alt="Knauer - Liquid Handler LH 8.1 - Sandwich Injection Mode" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Sandwich_Injection_Mode_Big_fb713e65a0.jpg 179w," sizes="100vw" width="2832" height="2475"></p><h5><strong>Full loop</strong></h5><ul><li>Sample loop is completely filled with the sample</li><li>Maximum reproducibility is achieved</li><li>Injection volume equals the loop volume</li><li>The sample loop will be overfilled to ensure a complete and reproducible filling</li></ul><h5><strong>Partial loop</strong></h5><ul><li>Sample loop is partially filled with programmed amount of sample&nbsp;</li><li>Injection mode is recommended for preparative tasks</li><li>Maximum injection volume equals 50 % of the loop volume</li><li>No sample loss</li></ul><h3><strong>Large number of samples for high-throughput applications</strong></h3><p><strong>The Robotic Cooler offers space for up to 390 x 1.5 ml standard sample vials while maintaining a stable temperature.&nbsp;</strong></p><ul><li>The Robotic Cooler compartment offers 6 places for sample racks. Two per drawer with a variety of options. One place can be used by a 60 x 1.5 ml vial rack, or 384 well plates or 96 well plates. Furthermore a double rack can be used to offer 130 x 1.5 mL vial positions, taking the total sample capacity to 390 x 1.5 ml vials.</li><li>Depending on the rail length a multitude of Robotic Coolers can be installed and used seperatly. Up to 4 Robotic Coolers on the longest rail.</li><li>The Robotic Cooler is software controlled, which enables a higher response speed and only open the doors so the injection syringe can reach the sample, to protect other samples from temperature fluctuations.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_cooled_tray_1_d2a6edf265.jpg" alt="Knauer: Liquid Handler LH 8.1 - high throughtput" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_cooled_tray_1_d2a6edf265.jpg 245w," sizes="100vw" width="1722" height="1040"></p><h3><strong>Speed up your injection</strong></h3><p><strong>The LH 8.1 is optimized to save time during injection and movement.</strong></p><ul><li>To safe time during the movement, the opening of the Robotic Cooler is software controlled and happens automatically.</li><li>To further speed up the injection, the sampler can apply the "overlapped injections" function, where the sample is already prepared inside the syringe.</li><li>Then the sample can be injected directly after the end of the previous run.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Method_overlapped_2_e94a888086.jpg" alt="Knauer: Liquid Handler LH 8.1 - Method_overlapped" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Method_overlapped_2_e94a888086.jpg 245w," sizes="100vw" width="1121" height="310"></p><h3><strong>Easy control via HPLC software&nbsp;</strong></h3><ul><li><strong>The LH 8.1 </strong>can be used just as comfortable as any HPLC Autosampler.</li><li>All parameters for recommended syringes, like injection speed, delay and sandwich build-up are already implemented and extensively tested in our Chromatography Software packages, so that you are ready to go!</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Method_overlapped_af91cd2ba5.jpg" alt="Knauer: Liquid Handler LH 8.1 - SW control" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Method_overlapped_af91cd2ba5.jpg 188w," sizes="100vw" width="928" height="771"></p>

KNAUER AZURA Autosampler AS 6.1L

KNAUER AZURA AS 6.1L autosampler with up to 768 samples, ultra-low carryover, and fast injection cycles for HPLC/UHPLC workflows.

<p><strong>The KNAUER AZURA® AS 6.1L is a high-performance autosampler designed for automated sample injection in HPLC/UHPLC systems, offering high capacity, precision, and flexibility for both routine and high-throughput applications.</strong></p><p>The <strong>autosampler supports:</strong></p><ul><li>up to <strong>768 sample positions (microtiter plates)</strong></li><li>up to <strong>108 standard 1.5 mL vials</strong></li></ul><p>A highly effective <strong>internal and external needle wash system</strong> minimizes carryover to extremely low levels. The system is also fast, with a <strong>complete injection cycle taking less than one minute</strong>, including needle washing.</p><p>Three <strong>injection modes</strong> are supported:</p><ul><li><strong>full loop filling</strong> (highest precision)</li><li><strong>partial loop filling</strong> (variable volumes)</li><li><strong>µl pickup</strong> (loss-free injection of small volumes)</li></ul><p>The integrated <strong>ILD™ (Intermediate Loop Decompression) valve</strong> ensures accurate high-pressure injections without sample dilution and reduces pressure spikes, protecting the column.</p><h4><span><strong>Key Features</strong></span></h4><ul><li><strong>Autosampler for HPLC/UHPLC</strong> systems</li><li><strong>Up to 768 sample positions</strong></li><li><strong>Up to 108 vials </strong>(1.5 mL)</li><li>Ultra-low <strong>carryover (&lt; 0.0003%)</strong></li><li><strong>Fast injection</strong> cycle (<strong>&lt; 1 min</strong>)</li><li><strong>3 injection modes</strong> (full loop, partial loop, µl pickup)</li><li>ILD™ technology for high-pressure injection</li><li>PASA™ (pressure-assisted sample aspiration)</li><li>Optional <strong>sample cooling (4–40 °C)</strong></li><li>Missing vial/plate detection</li><li>Supports precolumn derivatization</li></ul><h3><span><strong>Technical Specifications</strong></span></h3><h4><span><strong>Sample Injection</strong></span></h4><ul><li><strong>Injection volume range:</strong> 0.1 µl – 10 mL</li><li><strong>Precision:</strong><ul><li><strong>Full loop:</strong> &lt; 0.3% RSD</li><li><strong>Partial loop:</strong> &lt; 0.5% RSD</li><li><strong>µl pickup:</strong> &lt; 1.0% RSD</li></ul></li><li><strong>Accuracy:</strong> 0.2 µl (250 µl syringe)</li></ul><h4><span><strong>Performance</strong></span></h4><ul><li><strong>Carryover:</strong><ul><li>&lt; 0.0015% (standard)</li><li>&lt; 0.0003% (extended wash)</li></ul></li><li><strong>Injections per vial:</strong> <strong>max. 9</strong></li><li><strong>Cycle time:</strong><ul><li>7 s (same vial)</li><li>14 s (different vials)</li><li>&lt; 60 s total</li></ul></li></ul><h4><span><strong>Capacity &amp; Compatibility</strong></span></h4><ul><li><strong>Microtiter plates:</strong> ANSI SLAS standard</li><li><strong>Max vial/plate height:</strong> <strong>47 mm</strong></li></ul><h4><span><strong>Additional Features</strong></span></h4><ul><li>Headspace pressure (bubble prevention)</li><li>Precolumn derivatization</li><li>Sample tray cooling (optional, 4–40 °C)</li><li>Vial detection sensor</li></ul><h4><span><strong>Materials</strong></span></h4><ul><li>ETFE, PTFE, stainless steel, glass, Vespel</li><li>Wide chemical compatibility</li></ul><h4><span><strong>Control &amp; Communication</strong></span></h4><ul><li>LAN / Ethernet</li><li>Analog inputs</li><li>TTL inputs (start, stop, freeze)</li><li>Relay output (alarm, marker)</li></ul><h4><span><strong>Operating Conditions</strong></span></h4><ul><li><strong>Temperature:</strong> 10–40 °C</li><li><strong>Humidity:</strong> 20–80%</li></ul>

Products from category HPLC Autosamplers focused on HPLC from KNAUER - page 1

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