<p><strong>Quantifying steroids in biological matrices has always presented analytical challenges. Complex structures, isobaric interferences, and matrix effects often limit accuracy and throughput — demanding a new approach beyond incremental improvements.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg 245w," sizes="100vw" width="1024" height="512"></p><p>The<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149"> <strong>Thermo Scientific Stellar MS</strong></a> redefines how analysts approach <strong>steroid quantification</strong>, enabling rapid method optimization through advanced scanning flexibility, complementary fragmentation mechanisms, and enhanced selectivity.</p><p>Rather than focusing on a single metric, this application note demonstrates how researchers can <strong>quickly develop more effective methods</strong> by leveraging the full capability of the Stellar MS to characterize compounds comprehensively in a single acquisition.</p><h3><strong>A new paradigm for analytical quantitation</strong></h3><p>Traditional LC-MS approaches often rely on incremental improvements in sensitivity or speed.<br>Stellar MS instead provides a new analytical framework, designed to optimize every parameter of quantitative method creation.</p><p><strong>Key attributes include:</strong></p><ol><li><strong>PRM vs. SRM/MRM — improving instrument efficiency</strong><br>Parallel Reaction Monitoring (PRM) on Stellar MS provides comprehensive ion information for each target steroid, improving quantitation accuracy and efficiency over traditional MRM methods.</li><li><strong>Complementary dissociation mechanisms — HCD and CID</strong><br>Stellar’s unique ability to combine <strong>beam-type (HCD)</strong> and <strong>resonant (CID)</strong> dissociation ensures full coverage of all 17 targeted steroids. This dual-mode approach effectively overcomes neutral water loss product ions — one of the key challenges in steroid analysis.</li><li><strong>Next-level selectivity — MS² to MS³ transition</strong><br>The platform’s ability to perform <strong>MS³ experiments</strong> enhances selectivity and confidence, particularly in complex biological matrices, where interferences are common.</li><li><strong>Product ion accumulation vs. dwell time — improved ion ratios and sensitivity</strong><br>By accumulating product ions rather than relying on fixed dwell times, Stellar MS achieves superior ion ratio precision and reproducibility — even at low spiking levels — without sacrificing throughput.</li></ol><p>&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer: Figure 1. Representative EIC of 17 steroids in human serum using the Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg 245w," sizes="100vw" width="800" height="412"></p><h3><strong>From characterization to confident method creation</strong></h3><p>The power of Stellar MS lies in its <strong>comprehensive acquisition strategy</strong>. In this application note, a single injection per spiking level generated <strong>125 distinct scan events</strong>, combining:</p><ul><li>HCD and CID MS² experiments</li><li>Targeted MS³ scans</li><li>Positive/negative <strong>polarity switching</strong></li></ul><p><strong>This workflow enables analysts to rapidly evaluate:</strong></p><ol><li>Which <strong>dissociation mechanism</strong> produces the most informative spectra</li><li>Whether <strong>MS² or MS³</strong> is best suited for each compound</li><li>The optimal <strong>product ions</strong> for confident post-acquisition quantitation</li></ol><p>By providing this complete dataset in a single run, Stellar MS dramatically accelerates <strong>method optimization and validation</strong>, supporting more efficient and reliable quantitation strategies.</p><h3><strong>Driving method development efficiency</strong></h3><p>The real strength of the Stellar MS isn’t just its speed or sensitivity — it’s the ability to <strong>experiment, compare, and decide</strong> in one workflow.<br><br>Through simultaneous HCD/CID fragmentation, MS² and MS³ switching, and polarity control, users can determine the optimal analytical conditions across complex steroid panels quickly and confidently.</p><p><strong>This approach helps labs:</strong></p><ul><li>Reduce development time</li><li>Maximize data quality</li><li>Improve long-term robustness and reproducibility</li></ul><h3><strong>Conclusion</strong></h3><p>The <strong>Stellar MS</strong> platform empowers analytical scientists to move beyond incremental performance metrics toward <strong>methodological innovation</strong>.</p><p>By integrating comprehensive characterization and advanced scanning control, it enables faster, more confident decisions in quantitative steroid analysis — setting a new benchmark for flexibility and precision in LC-MS.</p><p><strong>Ask the Experts: On-Demand Webinar</strong></p><p>Learn more about how Stellar MS enhances steroid profiling and quantitative workflows.<br><strong>Watch the on-demand webinar where Thermo Fisher scientists discuss:</strong></p><ul><li>Advanced method development strategies</li><li>Insights from the steroid panel dataset</li><li>Practical examples of leveraging Stellar’s flexibility for confident quantitation</li></ul><blockquote><p><strong>Watch now: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5117"><strong>Unlocking the Power of Quantitative Analysis with Stellar MS</strong></a></p><p><strong>Read the full application note:</strong><i><strong> </strong></i><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/32415"><i><strong>Selective and sensitive measurement of 17 steroids in human serum using a Stellar mass spectrometer</strong></i></a></p></blockquote><ul><li>Learn more about<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/stellar-mass-spectrometer/stellar-mass-spectrometer-applications.html"><strong>Stellar Mass Spectrometer applications</strong></a></li><li><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a><strong>&nbsp;</strong>#MassSpectrometry #ClinicalResearch #LCMS #StellarMS</li></ul><blockquote><h5><strong>Dane Walker</strong></h5><p>Dane Walker is Regional Marketing Manager at Thermo Fisher Scientific, focused on LC-MS systems across EMEA. With a PhD in Surface Science and a background in scientific instrumentation, he blends technical expertise with strategic marketing to drive data-led campaigns. Passionate about communicating science and enabling innovation, Dane enjoys powerboating, cycling, stargazing, and talking endlessly about mass spectrometry.</p></blockquote>

Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer

<p><strong>Visit the LabRulez booth at </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871"><strong>analytica 2026</strong></a><strong> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"></p><h3><strong>Would you like to learn how the LabRulez portals work?</strong></h3><p>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/"><strong>LabRulezLCMS</strong></a><br>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/"><strong>LabRulezGCMS</strong></a><br>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/"><strong>LabRulezICPMS</strong></a></p><ul><li>A <strong>unique online solution</strong> for <strong>your marketing</strong></li><li>A <strong>targeted audience</strong> from the <strong>analytical chemistry community</strong></li><li><strong>Modern technologies</strong> and a unique concept</li><li><strong>Design optimized for most smart devices</strong> – PC, mobile, and tablet</li><li><strong>Advanced statistical tools</strong> fully available to users</li></ul><h3><strong>Services Offered</strong></h3><h4><strong>News + Company Updates</strong></h4><ul><li>Publish <strong>news related to your instrumentation, applications, services, events, job openings</strong>, and more</li><li><strong>News is shared</strong> both on our <strong>portals</strong> and across our <strong>social media channels</strong></li></ul><h4><strong>Library – All Documents in One Place</strong></h4><ul><li><strong>Applications, posters, presentations, technical articles, brochures, specifications</strong></li><li><strong>Fas</strong>t and <strong>advanced search</strong> capabilities</li><li>Over <strong>32,000 documents</strong> currently available in our database</li></ul><h4><strong>Webinars</strong></h4><ul><li><strong>Upcoming and on-demand webinars</strong> from the field of analytical chemistry</li><li>More than <strong>5,500 webinars</strong> covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4><strong>Careers</strong></h4><ul><li><strong>Targeted job</strong> listings in <strong>analytical chemistry</strong></li><li>Clear filtering by specialization or location</li><li><strong>Increased visibility for job postings</strong></li></ul><h4><strong>Companies</strong></h4><ul><li><strong>Company profile</strong> with key information and contacts</li><li><strong>Integration of all your activities on our portal:</strong> News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li><strong>Connection to your Products </strong>listed on LabRulez portals</li></ul><h4><strong>Products and Services</strong></h4><ul><li>Comprehensive <strong>overview of instrumental techniques and services</strong></li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4><strong>Marketplace</strong></h4><ul><li><strong>Buying and selling pre-owned analytical equipment</strong></li><li>We purchase analytical instrumentation of all brands and ages</li><li><strong>Sell your equipment through LabRulez</strong></li><li>Request and purchase <strong>complete systems, modules</strong>, or <strong>spare parts</strong> with warranty and service</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"></p><h3><strong>What Will Be on Display at Our Booth?</strong></h3><p>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.</p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar"><strong>LabRulezLCMS Marketplace</strong></a></h4><p><strong>Spare parts for LC/MS:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378"><strong>Vacuum turbo pumps for LC/MS</strong></a></li></ul><p><strong>Spare parts for HPLC:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397"><strong>Degassers for HPLC/UHPLC</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve"><strong>Automatic valves for HPLC</strong></a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul><p><strong>Consumables:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377"><strong>HPLC lamps</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48"><strong>HPLC columns</strong></a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar"><strong>LabRulezGCMS Marketplace</strong></a></h4><p><strong>Spare parts for GC/MS:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo"><strong>Turbo pumps for GC/MS</strong></a></li></ul><p><strong>Spare parts for GC:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC"><strong>EPC modules</strong></a></li></ul><p><strong>Sample injection</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432"><strong>Autosampler 7693A for GC</strong></a></li></ul><p><strong>Consumables:</strong></p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7"><strong>GC columns </strong></a>and selected accessories</li></ul>

LabRulez at analytica 2026

<h4><strong>The future of chromatography is here – are you ready?</strong></h4><p><strong>Chromatography continues to evolve at an unprecedented pace, driven by the need for sustainability, analytical performance, and digital innovation. This dedicated analytica 2026 conference session brings together leading experts to explore how next-generation GC and LC technologies, advanced hyphenated techniques, microfluidics, additive manufacturing, and data-driven workflows are reshaping analytical science. From greener HPLC strategies and supercritical fluid chromatography to ion mobility–HRMS and multidimensional separations, the programme provides a forward-looking perspective on how laboratories can adapt to emerging analytical challenges and unlock new scientific insights.</strong></p><blockquote><h5><strong>Companies participating in the analytica 2026 trade fair:</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1200"><strong>Agilent Technologies</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1206"><strong>ANALYTIKA</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1197"><strong>Bruker</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1201"><strong>DataApex</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1195"><strong>KNAUER</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7049"><strong>LabRulez</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1227"><strong>LECO</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1202"><strong>Markes International</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1214"><strong>Metrohm</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1213"><strong>PerkinElmer</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1216"><strong>Restek</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1196"><strong>SCIEX</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1230"><strong>SciY</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1215"><strong>Shimadzu</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1191"><strong>Thermo Fisher Scientific</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1217"><strong>Wasson-ECE</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1228"><strong>Waters Corporation</strong></a></li></ul></blockquote><h4><strong>Programme Tuesday, 24 March 2026, ICM Saal 5</strong></h4><h5><strong>09:30 - 10:00 EuChemS-DAC Tribute to Prof. Dr. Wolfgang Buchberger presented by Dr. Martin Vogel</strong></h5><ul><li>Dr. Martin Vogel | Germany | University of Münster</li></ul><h4><strong>The Future of Chromatography Is Here – Are You Ready? Towards Sustainable HPLC: Are Narrower Columns the Solution? (GDCh)</strong></h4><h5><strong>10:00 - 10:30 From filament to chromatogram: using additive manufacturing to democratise miniaturisation and promote sustainability in analytical laboratories</strong></h5><ul><li>Dr. Thorsten Teutenberg | Germany | Institut für Umwelt &amp; Energie, Technik &amp; Analytik e.V. (IUTA)</li></ul><h5><strong>10:30 - 11:00 Supercritical fluid chromatography as a sustainable analytical method</strong></h5><ul><li>Prof. Dr. Caroline West | France | University of Orleans</li></ul><h5><strong>11:00 - 11:30 Microfluidic versus conventional LC–HRMS for HIV metabolomics: a comparative performance assessment</strong></h5><ul><li>Frederic Lynen | Belgium</li></ul><h5><strong>11:30 - 12:30 Poster Session sponsored by Shimadzu</strong></h5><h4><strong>The Future of Chromatography Is Here – Are You Ready? GC &amp; HPLC: The Cutting-Edge Advances You Need to Know - Part I (GDCh)</strong></h4><h5><strong>12:30 - 13:00 Next-Gen HPLC: A Column Technology Deep Dive</strong></h5><ul><li>Prof. Dr. Gert Desmet | Belgium | Free University Brussels</li></ul><h5><strong>13:00 - 13:30 GC-Based hyphenated techniques for detailed characterization of complex samples</strong></h5><ul><li>Prof. Dr. Giorgia Purcaro | Belgium | University of Liège</li></ul><h5><strong>13:30 - 14:00 Molecular Encoding as a Tool to Enable Data Science-Assisted Analytical Measurements</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/articles?search=Kevin+Schug"><strong>Prof. Kevin Schug</strong></a> | USA | The University of Texas at Arlington</li></ul><h5><strong>14:00 - 14:30 Good Resolutions for Bad Pollutants: Ion Mobility–HRMS to Resolve Complex Environmental Mixtures</strong></h5><ul><li>Prof. Dr. Gauthier Eppe | Belgium</li></ul><h4><strong>The Future of Chromatography Is Here – Are You Ready? GC &amp; HPLC: The Cutting-Edge Advances You Need to Know - Part II (GDCh)</strong></h4><h5><strong>15:00 - 15:30 GC×GC-TOFMS for Medical Volatolomics</strong></h5><ul><li>Prof. Dr. Jean-François Focant | Belgium | University of Liege</li></ul><h5><strong>15:30 - 16:00 Cryogenic band compression as a means to simplify the sample preparation step in 1D and comprehensive 2D GC food analysis</strong></h5><ul><li>Prof. Dr. Peter Q. Tranchida | Italy | University of Messina</li></ul><h5><strong>16:00 - 16:30 Significance of radial dispersion to effective modulation in two-dimensional liquid chromatography and its role in machine-learning based automated method development</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/articles?search=bob+pirok"><strong>Bob Pirok</strong></a> | Netherlands | University of Amsterdam</li></ul><h5><strong>16:30 - 17:00 LCxSFC coupling: A robust tool to aid understanding of renewable and circular industrial processes&nbsp;</strong></h5><ul><li>Karine Faure | France | University of Lyon</li></ul>

Analytica 2026: The Future of Chromatography Is Here – Are You Ready?

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laboratory technology, analysis, biotechnology and analytica conference

For five decades, the world's leading trade fair analytica has been your guarantee of success for the successful presentation of innovative laboratory technology and pioneering biotechnology. As the most important meeting place for the sector, it brings together the complete range of topics for laboratories in industry and research.

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LabRulez

Thermo Scientific mzCloud Mass Spectral Library

mzCloud is a high-resolution, accurate-mass MSⁿ spectral library that helps identify unknown compounds when no reference standards are available, especially in complex studies.

<div colctrl-id="1-1"><div><div container-id="cq-colctrl-lt8" comp-id="B1"><div><h3><strong>Confident identification for unknown small molecules</strong></h3></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div><p><strong>Mass spectral libraries and databases may be required to identify unknown compounds when reference standards are not available. This is particularly true for studies in areas such as metabolomics, forensic toxicology, food &amp; beverage, environmental emerging contaminants or extractables &amp; leachables.</strong></p></div></div></div></div><div><p>In these situations, a mass spectral library, particularly a high-resolution and accurate-mass MS<sup>n</sup> fragmentation library, such as the mzCloud mass spectral library, can provide the information required to confidently identify unknown compounds.</p><h3><strong>Key benefits</strong></h3><h5><strong>Confidence for unknown identification</strong></h5><p>Comprehensive curation of all library content with extensive, fully annotated and searchable fragmentation spectra to provide confidence in your results.</p><h5><strong>Know more unknowns&nbsp;&nbsp;&nbsp;&nbsp;</strong></h5><p>Identify unknowns with automated deconvolution and library searching against extensive fragmentation spectra covering a wide chemical space, including small molecule applications.</p><h5><strong>From spectra to structure&nbsp;&nbsp;</strong></h5><p>With solutions to cover infusion or LC-MS, nominal or high-resolution data, you can be sure that your MS<sup>n</sup> spectra will provide you with actionable results, even when there is no spectral library match.</p><h5><strong>Flexibility for research and routine applications</strong></h5><p>Search for free online at <a target="_blank" rel="noopener noreferrer" href="http://www.mzcloud.org/" aria-label="mzCloud.org - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="http://www.mzcloud.org/$mzcloud.org"><span style="white-space:nowrap;">mzCloud.org</span></a>, or directly access through the Thermo Scientific Compound Discoverer and Mass Frontier software packages. Not online? mzVault provides you with the ability to perform offline spectral library searching.</p><figure class="image"><img style="aspect-ratio:1398/1002;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_ecc79fe9bf.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: mzCloud is easily and freely accessed online. Anyone can search, review, and use its extensive information. Note that the numbers above were correct at time of publication. For the most up to date numbers visit mzCloud.org." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_ecc79fe9bf.jpg 218w," sizes="100vw" width="1398" height="1002"></figure><h3><strong>What is in the mzCloud mass spectral library?</strong></h3><div style="height:auto;" id="whatismzcloud"><div><div><div><div><p>mzCloud is an extensively curated, high-quality mass spectral fragmentation library that contains significant amounts of spectra and metadata for each entry. Each entry also includes exhaustive high-resolution <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/mass-spectral-libraries/mzcloud-mass-spectral-library.html?open=multi-stage-fragmentation#multi-stage-fragmentation">MS/MS and multi-stage MS<sup>n</sup> spectra</a>, which have been acquired at various collision energies and using different fragmentation techniques.</p><p><strong>Each raw mass spectrum</strong> has been filtered to remove noise, recalibrated to ensure absolute mass accuracy, fully annotated and <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/mass-spectral-libraries/mzcloud-mass-spectral-library.html?open=content-curation#content-curation">curated</a> to ensure quality and consistency.</p><p>Finally, the contents of the library are <strong>updated weekly</strong> to provide coverage of a wide chemical space, ensuring relevance to all small-molecule application areas.</p></div><div><figure class="image"><img style="aspect-ratio:740/410;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_2_77a6795676.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: Example of the Mass Frontier software utilizing the contents of mzCloud to identify an unknown compound through a sub-structural search; the experimental fragmentation spectrum (top left) is searched against the mzCloud mass spectral database, with corresponding spectral hits shown in a given spectral tree (top, middle), with supporting spectral information (bottom left) and the identified precursor structure from the matched fragmentation spectra (bottom right), and the library compound structure from which the fragment originated (top right)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_2_77a6795676.jpg 245w," sizes="100vw" width="740" height="410"></figure></div><div><p>Distinguishing among types of databases is important, as the contents and quality of the contents typically differs significantly and may have an impact upon the reliability of the data and the confidence in any results obtained.</p><ul><li><strong>Chemical database</strong>: A collection of chemical information with, at minimum, a name and a formula. It may also include structures, elemental composition, retention time information, and additional biological or chemical metadata.</li><li><strong>Accurate-mass (AM) database</strong>: An AM database contains accurate mass formulas.</li><li><strong>Accurate-mass retention-time (AMRT) database</strong>: An AMRT database contains accurate mass and retention time(s) for specific analytical methods.</li><li><strong>Library</strong>: A library is a database (AM or AMRT) that, in addition to the above, also contains reference mass spectral data, MS/MS, and/or MS<sup>n</sup> data on compounds.</li></ul><h3><strong>Multi-stage fragmentation in the mzCloud mass spectral library</strong></h3><div style="height:auto;" id="multistagefragmentation"><div><div><div><div><figure class="image"><img style="aspect-ratio:740/420;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_3_792a9d9a59.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: A schematic representation of a spectral tree within mzCloud. The MS spectra are acquired for a given compound in multiple polarities (ESI +/-), and for a range of adducts. Each precursor is exhaustively fragmented using different fragmentation techniques (CID, HCD) and at multiple collision energies to produce collections of fragmentation spectra at each fragmentation level (MS2, MS3, MS4 etc.), generating a comprehensive spectral tree of information for each library entry." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_3_792a9d9a59.jpg 245w," sizes="100vw" width="740" height="420"></figure><p>mzCloud is an<strong> all-encompassing mass spectral library </strong>with content based on a wide chemical space (therefore covering all small-molecule application areas), and acquiring high-resolution and accurate-mass data at various collision energies, for multiple adducts, using CID and HCD. Approximately 10–20 collision energies are included for every precursor ion; this multi-stage, multidimensional MS<sup>n</sup> spectral data are arranged into spectral trees, which are both extensive and comprehensive, typically containing many hundreds to thousands of spectra for each compound, with many exceeding MS<sup>4</sup> fragmentation steps.</p><p>The extensive data for each library entry is critical for accurate compound identifications, matching experimentally obtained data to that of the library contents, with fit confidence and data visualization <strong>provided in the</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759"><strong> Compound Discoverer</strong></a><strong> and </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757"><strong>Mass Frontier</strong></a><strong> data analysis software.</strong> Additional tools in Compound Discoverer and Mass Frontier software, including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1762"><strong>mzLogic</strong></a>, use the extensive fragmentation information to confidently identify unknowns that cannot be identified based upon the spectral library compound entries alone.</p><h3><strong>Content curation in the mzCloud mass spectral library</strong></h3><div style="height:auto;" id="contentcuration"><div><div><div><div><figure class="image"><img style="aspect-ratio:740/342;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_4_c7064c2ca2.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: An example of the mass spectral content curation tool available within the Mass Frontier software. It contains all the functionality used to manually curate the content contained within mzCloud. The power of mzCloud lies in both the extent and high quality of its content, which helps make confident compound identifications." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_4_c7064c2ca2.jpg 245w," sizes="100vw" width="740" height="342"></figure><p>Despite the fact that <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=orbitrap"><strong>Thermo Scientific Orbitrap-based mass analyzers</strong></a> routinely deliver sub 3-ppm mass errors, there is a small amount of inaccuracy in any experimental data used to search against a library; these errors would be compounded if the library itself also contained data with mass errors. The compounded errors would mean that any identifications could be harder to make or have lower confidence due to match scores being higher.</p><p>Curating the content within mzCloud guards against error and variability in the reference spectra against which data is compared. <strong>The process of curation involves:</strong></p><ul><li>Averaging multiple, individually acquired spectra</li><li>The removal of mass spectral noise</li><li>Recalibration of the mass spectra based upon the predicted formulas for the fragment ion elemental compositions, based upon the known elemental composition and structure of the precursor ion</li><li>Annotation of all fragment ions with the fragment structure</li><li>Addition of large amounts of metadata, including the source of the data and all original raw data files</li></ul><p>This curation process is performed <strong>for every spectra</strong> - every collision energy, every fragmentation type, every MS<sup>n</sup> level and for each precursor ion. As such, the spectral data in mzCloud is of <strong>exceedingly high quality </strong>and offers significant advantages over crowd-sourced libraries due to the high levels of control.</p><figure class="image"><img style="aspect-ratio:740/350;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_5_0c3fdc4c6c.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: A recalibrated fragmentation spectrum showing no error on the highlighted peak at m/z 213.05462, along with all of the annotation that is provided for each spectra, at each collision energy, for each fragmentation type at each MSn level." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_5_0c3fdc4c6c.jpg 245w," sizes="100vw" width="740" height="350"></figure><div><div><div container-id="cq-colctrl-lt8" comp-id="B1"><div><h3><strong>How can I use the mzCloud mass spectral library?</strong></h3><p>Accelerate your small molecule unknown identification studies with <strong>mzCloud</strong>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1762"><strong>mzLogic</strong></a>, and the other data analysis and mass spectral prediction tools available in<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759"><strong>Compound Discoverer</strong></a> software and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757"><strong>Mass Frontier</strong></a><strong> </strong>software.</p><p><img style="aspect-ratio:860/261;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_7_a8cefb0028.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: Connectivity of mzCloud and mzVault to Mass Frontier." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_7_a8cefb0028.jpg 245w," sizes="100vw" width="860" height="261"></p><p>The content within mzCloud is accessible through Compound Discoverer and Mass Frontier software packages, provided you have an internet connection. No internet? No problem – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1760"><strong>mzVault</strong></a>, provides you with the MS and MS/MS content of mzCloud in an offline format.</p><div style="height:auto;" id="accessmzcloud"><div><div><div><div colctrl-id="3-1"><div><div container-id="cq-colctrl-lt8" comp-id="B1"><div><p><img style="aspect-ratio:860/242;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_6_ae3c0a28e1.jpg" alt="Thermo Scientific mzCloud Mass Spectral Library: Connectivity of mzCloud and mzVault to Compound Discoverer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_mz_Cloud_Mass_Spectral_Library_6_ae3c0a28e1.jpg 245w," sizes="100vw" width="860" height="242"></p><p>Due to security concerns or local firewalls, not everyone can connect processing PCs to the internet. With that in mind, the contents of mzCloud are combined into an offline version twice a year. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1760"><strong>mzVault</strong></a><strong> </strong>software application helps you access this information. In addition to accessing the offline version of mzCloud, the mzVault application can store and search your high-quality custom in-house (offline) MS/MS spectral libraries.</p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

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