<p><strong>Cells in the human body constantly evaluate signals from their surroundings and decide whether to divide, grow, or remain inactive. One of the key systems controlling this communication is the so-called Wnt signalling pathway. If this mechanism becomes disrupted, cells can spiral out of control, leading to uncontrolled growth and the development of tumours. Scientists from CEITEC and the Faculty of Science at Masaryk University have now described a critical decision-making process – the point at which a cell’s future development is decided. Their findings may, in the future, contribute to the search for ways to slow down or stop such processes in diseases like cancer.</strong></p><p>The <strong>Wnt signalling pathway</strong> is a mechanism that coordinates billions of cells in the body – it is responsible for the development of an entire organism from a single cell, and it continues to regulate tissue renewal throughout adult life. Until now, it was not fully understood how this mechanism works in detail. However, new research by teams from Masaryk University (MUNI) shows that the cells do not respond to stimuli smoothly, but rather operate in an on-off mode once a certain decision-threshold is crossed. This principle changes our understanding of how cells function together and helps explain situations in which cellular decision-making goes out of control and may lead to cancer development.</p><p>The process begins at the surface of the cell, where a signal is captured by a receptor embedded in the cell membrane. A protein called Dishevelled (DVL) then binds to this receptor and carries the signal further into the cell. However, for the signal to continue, DVL must first undergo a series of chemical modifications. Small chemical groups begin to accumulate on its surface, progressively increasing its negative charge.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_4_7c8f80376d.jpg" alt="CEITEC/Tripsianes Lab: Scientists Uncover a Tipping Point in Cells. This Is How Cancer May Begin: New mechanism of Wnt signal transduction " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_4_7c8f80376d.jpg 245w," sizes="100vw" width="1600" height="604"></p><p>And this is where the<strong> decisive moment occurs</strong>.</p><p><i>“We found that the DVL protein behaves like a switch – its structure changes abruptly once the negative charge on its surface exceeds a certain threshold. Only then does it rearrange and allow the signal to proceed further,”</i> explains first author <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/miroslav-micka/u92423?page=publication"><strong>Miroslav Micka</strong></a>.</p><p>At this point, DVL detaches from the receptor, allowing the signal to move deeper into the cell. The cell then receives a clear instruction on how to respond – for example, to start dividing or change its function.</p><p>This crucial “tipping point,” where the fate of the signal is decided, has so far been only partially understood: <i>“Scientists knew the pathway the signal follows, but they mostly viewed it as a gradual regulatory system. However, our teams have shown that it is rather a process based on decision points and abrupt changes once a certain threshold is crossed. This is important not only for understanding how cells function, but also for explaining what happens when these processes become dysregulated,”</i> add corresponding authors <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/konstantinos-tripsianes-ph-d/u17552?page=publication"><strong>Konstantinos Tripsianes</strong></a><strong> from CEITEC MUNI</strong> and <a target="_blank" rel="noopener noreferrer" href="https://www.muni.cz/en/people/11088-vitezslav-bryja"><strong>Vítězslav Bryja</strong></a><strong> from the Faculty of Science</strong>.</p><p>Such decision points in cellular signalling are particularly important from a medical perspective, as their disruption can trigger processes that lead to tumour development. A better understanding of these processes, on the other hand, paves the way for more effective treatments.</p><p>The study was published in the journal <a target="_blank" rel="noopener noreferrer" href="https://www.science.org/doi/10.1126/sciadv.aed8899"><strong>Science Advances</strong></a>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_2_af629ac80a.jpg" alt="CEITEC/Tripsianes Lab: Scientists Uncover a Tipping Point in Cells. This Is How Cancer May Begin: DVL in a cell (green), cell nucleus (blue)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_2_af629ac80a.jpg 156w," sizes="100vw" width="1600" height="1600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_36bfbfa0c6.jpg" alt="CEITEC/Tripsianes Lab: Scientists Uncover a Tipping Point in Cells. This Is How Cancer May Begin: DVL in a cell (green), enzym modifiyng DVL (red), cell nucleus (blue)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_36bfbfa0c6.jpg 156w," sizes="100vw" width="1600" height="1600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_3_f21d18e3ca.jpg" alt="CEITEC/Tripsianes Lab: Scientists Uncover a Tipping Point in Cells. This Is How Cancer May Begin: DVL in a cell (green), enzym modifiyng DVL (red), cell nucleus (blue)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vedci_nasli_bod_zlomu_v_bunce_Prave_takto_muze_zacit_rakovina_3_f21d18e3ca.jpg 156w," sizes="100vw" width="1600" height="1600"></p><p>&nbsp;</p><blockquote><h4><strong>Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.science.org/doi/10.1126/sciadv.aed8899"><strong>A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling</strong></a></h4><p>Miroslav Micka, Jitender Kumar, Petra Paclíková, Zuzana Hayek, Kateřina Hanáková, Cherine Bechara, Hana Plešingerová, Ondrej Šedo, Sara Bologna, Elise Del Nero, Kristína Gömöryová, Vojtěch Bystrý, Tomáš Gybel, Tereza Čihalová, Marek Kravec, David Potěšil, Zbyněk Zdráhal, Konstantinos Tripsianes a Vítězslav Bryja.</p><p><i><strong>Sci. Adv., 2026, 12(20), eaed8899</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1126/sciadv.aed8899">DOI: 10.1126/sciadv.aed8899</a></p><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>It is well known that Dishevelled (DVL) phosphorylation by casein kinase 1 (CK1) relays Wnt signals from Frizzled (FZD) receptors to downstream effectors, yet any mechanistic aspects of DVL function related to phosphorylation remain unresolved. Here, we uncovered a Wnt-induced DVL phospho-switch that is mutually exclusive with FZD association. CK1 multiphosphorylation changes dramatically the bulk electrostatics to promote DVL intramolecular interaction between the DEP domain and the adjacent disordered region. A panel of DVL3 mutants demonstrated a switch-like behavior at the molecular level when a charge threshold was reached. Charge accumulation proximal to DEP proved to be a key functional event required, but not sufficient, for Wnt/β-catenin signaling. Proximity interactomics revealed FZD receptors as the prominent effectors of the DVL phospho-switch function consistent with the molecular competition at the DEP interface. By integrating findings at different levels, we propose a universal mechanism, in which Wnt-induced DVL conformational phospho-switch attenuates coresidence with FZDs as a means for downstream signaling events.</p>

 Scientists Uncover a Tipping Point in Cells. This Is How Cancer May Begin 

<div><div><p><strong>Deoxyribozymes represent a new frontier in biotechnology. They show that DNA is much more than a storage molecule for genetic information: it can also function as a catalytic machine. Unlike traditional enzymes made of proteins, such DNA-based tools are stable, easy to produce in large quantities, and can be evolved in a lab to perform different chemical tasks we need. Their potential is vast, ranging from low-cost diagnostic kits to high-speed screening tools that can facilitate drug discovery. As our ability to understand and predict their behavior continues to improve, these versatile molecules are becoming a cornerstone for future personalized medicine and environmental monitoring.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Deoxyribozymes_tools_for_precision_sensing_and_machine_learning_guided_discoveries_e771c8c360.jpeg" alt="IOCB Prague: Deoxyribozymes, tools for precision sensing and machine learning-guided discoveries: Researchers described a new assay for DNA-editing enzymes in which the substrate in the reaction is a chemiluminescent deoxyribozyme called Supernova. ACS Chem. Biol. 2026, 21, 5, 941-950" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Deoxyribozymes_tools_for_precision_sensing_and_machine_learning_guided_discoveries_e771c8c360.jpeg 245w," sizes="100vw" width="1049" height="561"></p></div></div><div><p>Researchers led by <a target="_blank" rel="noopener noreferrer" href="https://curtis.group.uochb.cz/en"><strong>Edward Curtis</strong></a> from IOCB Prague have developed a remarkable DNA molecule called Supernova that acts as a tiny biological flashlight, glowing blue during a specific chemical reaction. By tweaking its sequence, scientists transformed Supernova into a sensor capable of detecting DNA-editing enzymes, which are proteins that alter the sequence of DNA and are often linked to the development of cancer. These new sensors represent a step forward because they are roughly three times faster and over five times cheaper than standard assays. Depending on how they are designed, these sensors can be either "turned off" or "turned on" by a DNA-editing enzyme in a sample. Such sensors will make characterization of DNA-editing enzymes easier, and could also help scientists to find new inhibitors.</p><div><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Deoxyribozymes_tools_for_precision_sensing_and_machine_learning_guided_discoveries_2_8e228a1903.jpeg" alt="IOCB Prague: Deoxyribozymes, tools for precision sensing and machine learning-guided discoveries: Researchers also systematically investigated the specificities of self-phosphorylating deoxyribozymes that convert the coumarin substrate 4-MUP into a fluorescent product using biochemical assays, single-step selections, and machine learning. ACS Chem. Biol. 2026, 21, 5, 951-959" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Deoxyribozymes_tools_for_precision_sensing_and_machine_learning_guided_discoveries_2_8e228a1903.jpeg 245w," sizes="100vw" width="1561" height="505"></p><p>In another advance, researchers in the Curtis Group used a fluorescent deoxyribozyme named Aurora to explore how these molecular tools can be taught to recognize specific chemical targets. Using a combination of artificial evolution and machine learning, the team successfully identified specific mutations that change Aurora’s preference for one substrate over another. Machine learning is particularly powerful here because it allows researchers to predict how millions of different DNA variants will behave without having to test each one individually in a lab. This approach helps us understand the fundamental rules of how DNA molecules interact with the world, which is essential for designing more precise medicines and diagnostic tools.</p><h3><strong>These results are described in two recent publications:</strong></h3><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acschembio.5c00927"><strong>Chemiluminescent Deoxyribozyme Sensors for DNA-Editing Enzymes</strong></a></h4><p>Jakubec, M.; Svoboda, M.; Kurfürst, J.; Svehlova, K.; Veverka, V.; Curtis, E. A. &nbsp;</p><p><i>ACS Chem. Biol. </i><strong>2026</strong></p><p><a target="_blank" rel="noopener noreferrer" href="http://doi.org/10.1021/acschembio.5c00927">http://doi.org/10.1021/acschembio.5c00927</a></p><h6><strong>licensed under </strong><a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>DNA-editing enzymes such as those in the APOBEC family of cytidine deaminases play important roles in both normal and pathogenic function, while engineered enzymes offer exciting new possibilities for genome editing. Despite their importance, widely used assays for DNA-editing enzymes are time-consuming and expensive. Here, we describe a new assay for DNA-editing enzymes in which the substrate in the reaction is a chemiluminescent deoxyribozyme called Supernova. Editing alters the sequence of Supernova, which results in a change in catalytic activity and light production. By analyzing a data set of Supernova variants previously identified by selection and high-throughput sequencing, it was possible to generate sensors with a wide range of specificities. Sensors were also developed for APOBEC3A, a cytidine deaminase which converts C to U in single-stranded DNA and RNA. These include a turn-off sensor that produces light 14-fold slower after incubation with recombinant APOBEC3A than in its absence, and a turn-on sensor that generates light 10-fold faster after incubation with APOBEC3A than in its absence. Assays that use these sensors are faster and less expensive than existing ones, and should be particularly useful for applications such as high-throughput screening.</p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acschembio.5c00969"><strong>Probing the Specificity of Fluorescent Deoxyribozymes Using Single-Step Selections and Machine Learning</strong></a></h4><p>Král’ová, Z.; Isler, L.; Volek, M.; Jandová, M.; Kurfürst, J.; Curtis, E. A.&nbsp;</p><p><i>ACS Chem. Biol. </i><strong>2026</strong>&nbsp;</p><p><a target="_blank" rel="noopener noreferrer" href="http://doi.org/10.1021/acschembio.5c00969">http://doi.org/10.1021/acschembio.5c00969</a></p><h6><strong>licensed under </strong><a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>The ability of proteins and nucleic acids to form specific binding sites for ligands is critical for biological function, and methods to modulate biochemical specificity are important for fields such as enzyme engineering and drug design. Here, we systematically investigated the specificities of self-phosphorylating deoxyribozymes that convert the coumarin substrate 4-MUP into a fluorescent product using biochemical assays, single-step selections, and machine learning. Activity assays using a panel of 20 catalytic motifs and 10 substrates that generate different types of signals when they are dephosphorylated revealed that these deoxyribozymes are extremely specific for 4-MUP. To identify mutations that change specificity, we constructed a library based on a self-phosphorylating fluorescent deoxyribozyme called Aurora. A series of single-step selections yielded variants that react with 4-MUP and the structurally similar substrate diFMUP, but not with the more distinct substrates pNPP and ELF. Pairwise analysis of sequences in the 4-MUP and diFMUP data sets revealed four mutations that modulate Aurora specificity. The effects of these mutations were confirmed using biochemical assays and could be predicted using models developed by machine learning. Taken together, our results show how single-step selections can be used to identify mutations that change the specificity of a deoxyribozyme. They also highlight how machine learning can be used to model complex data sets from <i>in vitro</i> selection experiments.</p></div></div>

Deoxyribozymes, tools for precision sensing and machine learning-guided discoveries

<p><strong>🎤Kelly Broster McMahon (Senior Manager of Market Development and Collaborations at Thermo Fisher Scientific).&nbsp;</strong></p><p>Kelly has over 15 years of experience in LC-MS and protein mass spectrometry. Her work focuses on translating complex analytical challenges into scalable, compliant workflows for the biopharmaceutical industry.</p><p><strong>In this episode of Concentrating on Chromatography, we sit down with Kelly Broster McMahon to explore the analytical science behind one of the most important pharmaceutical trends today: GLP-1 therapeutics.</strong></p><p>Drugs like Semaglutide have transformed the treatment of diabetes and obesity—but ensuring their safety, efficacy, and quality requires cutting-edge analytical workflows.</p><p>In this conversation, we break down how chromatography and high-resolution mass spectrometry (LC-MS) work together to characterize complex peptide therapeutics, detect trace-level impurities, and support drug development from early research through regulated manufacturing.</p><h4><strong>What You’ll Learn</strong></h4><ul><li>Why GLP-1 drugs are uniquely challenging to analyze</li><li>How LC-MS enables detailed peptide characterization</li><li>The importance of detecting low-level impurities for patient safety</li><li>How analytical workflows scale from R&amp;D to manufacturing</li><li>The role of software and data platforms in modern labs</li><li>Where chromatography and mass spectrometry are heading next</li></ul><h4><strong>Key Takeaways</strong></h4><ul><li>GLP-1 therapeutics are a “proof point” for the future of analytical chemistry—where success depends not on measuring more, but on measuring the right things earlier, with confidence.</li><li>As molecules become more complex, integrated workflows combining chromatography, high-resolution mass spectrometry, and advanced informatics are becoming essential to ensure data integrity, regulatory compliance, and ultimately, patient safety.</li></ul><h3><strong>Video Transcription</strong></h3><p>In this episode, Kelly, Senior Manager of Market Development and Collaborations at Thermo Fisher Scientific, discusses the analytical challenges associated with GLP-1 peptide therapeutics and explains how modern chromatography, high-resolution mass spectrometry, software, and data platforms are shaping the future of biopharmaceutical development. Drawing on more than 15 years of experience in LC-MS and protein mass spectrometry, she shares insights into peptide characterization, impurity profiling, workflow continuity, and the growing role of AI in analytical science.</p><h4><strong>Kelly's Background in Biopharmaceutical Analysis</strong></h4><p>Kelly currently focuses on biopharmaceutical development at Thermo Fisher Scientific, working closely with industry partners to transform complex analytical challenges into scalable and compliant workflows. Her scientific background includes cancer biology, quantitative proteomics, biomarker assays, systems oncology, and mass spectrometry-based drug discovery. Throughout her academic and industrial career, she has applied mass spectrometry to support patient stratification, treatment monitoring, and therapeutic development.</p><h4><strong>Why GLP-1 Therapeutics Are So Important Right Now</strong></h4><p>GLP-1 drugs have become one of the most discussed therapeutic classes because they occupy a unique space between traditional small molecules and large biologics. Their structural complexity, sensitivity to degradation, and global manufacturing scale make them an ideal example of how analytical science must evolve.</p><p>According to Kelly, these molecules require greater analytical sensitivity, deeper structural characterization, and seamless workflows that support everything from discovery through manufacturing and regulatory submission.</p><h4><strong>Analytical Challenges of Peptide Therapeutics</strong></h4><p>Unlike conventional small molecules, peptide drugs such as semaglutide are larger, conformationally dynamic, and often chemically modified to improve stability, half-life, and absorption.</p><p>These therapeutics may contain:</p><ul><li>Sequence variants</li><li>Oxidation products</li><li>Deamidation products</li><li>Aggregates</li><li>Trace-level impurities</li></ul><p>Many of these species are difficult to separate chromatographically and challenging to identify confidently. As a result, peptide characterization requires both highly selective chromatography and high-resolution accurate-mass spectrometry.</p><h4><strong>What GLP-1 Drugs Teach Us About the Future of Analytical Science</strong></h4><p>Kelly emphasized that the future of analytical science is not necessarily about generating more data but about measuring the right attributes earlier and with greater confidence.</p><p>GLP-1 developers increasingly require integrated analytical workflows that generate actionable insights during discovery while remaining robust enough to support late-stage development, manufacturing, and global regulatory filings. This trend highlights the growing importance of scalable and harmonized analytical ecosystems.</p><h4><strong>Why Small Molecular Changes Matter</strong></h4><p>Even a single amino acid substitution or post-translational modification can dramatically alter a peptide's biological behavior.</p><p>Minor structural changes may affect:</p><ul><li>Receptor binding</li><li>Potency</li><li>Stability</li><li>Immunogenicity</li></ul><p>Because these changes can directly influence safety and efficacy, regulatory agencies require precise structural characterization and control throughout development and manufacturing.</p><h4><strong>The Power of Combining Chromatography and Mass Spectrometry</strong></h4><p>For complex peptide therapeutics, chromatography and mass spectrometry work together to provide comprehensive characterization.</p><p>Chromatography separates complex mixtures and resolves closely related species, while high-resolution mass spectrometry provides molecular specificity and structural confirmation. Together, advanced UHPLC and HRAM MS deliver the selectivity, sensitivity, and confidence required for modern peptide analysis.</p><h4><strong>The Value of High-Resolution Accurate-Mass Spectrometry</strong></h4><p>Kelly described high-resolution accurate-mass (HRAM) mass spectrometry as an indispensable technology for drug development.</p><p>Compared with older analytical approaches, HRAM provides:</p><ul><li>Exact mass measurements</li><li>Isotopic resolution</li><li>Structural confirmation</li><li>Trace impurity detection</li><li>Identification of sequence variants</li><li>Characterization of post-translational modifications</li></ul><p>These capabilities enable confident data generation that can withstand regulatory scrutiny, audits, and global submissions.</p><h4><strong>Why Sensitivity Is Critical</strong></h4><p>The distinction between a safe and unsafe therapeutic can exist at extremely low concentration levels.</p><p>Detecting impurities at parts-per-billion levels allows developers to identify risks early in development and address them before manufacturing scale-up. Analytical sensitivity therefore supports both regulatory confidence and patient safety.</p><h4><strong>Resolving Co-Eluting Impurities</strong></h4><p>One of the greatest challenges in peptide analysis is distinguishing nearly identical molecular species.</p><p>By combining ultra-high-resolution mass spectrometry with optimized chromatographic separations, scientists can accurately differentiate closely related compounds and remove uncertainty from impurity profiling. This capability is essential for both regulatory submissions and therapeutic safety assessments.</p><h4><strong>Workflow Continuity Across Drug Development</strong></h4><p>Kelly stressed that analytical methods should not be reinvented at every stage of development.</p><p>Instead, workflows should transition smoothly from:</p><ul><li>Research and discovery</li><li>Development</li><li>Quality control</li><li>Manufacturing</li></ul><p>Maintaining continuity improves reproducibility, preserves data integrity, and enables scalable development without disrupting analytical performance.</p><h4><strong>The Evolution of Instruments, Software, and Data Platforms</strong></h4><p>Modern analytical ecosystems extend beyond LC-MS instrumentation alone.</p><p>Today's workflows integrate:</p><ul><li>Consumables</li><li>Chromatography systems</li><li>Mass spectrometers</li><li>Informatics platforms</li></ul><p>Instrumentation continues to become faster, more sensitive, and capable of higher throughput, while advanced software transforms complex datasets into actionable insights. These integrated platforms support compliance, traceability, and harmonized global operations.</p><h4><strong>Data Integrity and Regulatory Compliance</strong></h4><p>As drug development becomes increasingly global, maintaining data integrity is more important than ever.</p><p>Validated software systems, secure data management tools, and comprehensive audit trails help organizations harmonize workflows across multiple sites while ensuring that generated data can withstand regulatory review worldwide.</p><h4><strong>Lessons for Next-Generation Biologics</strong></h4><p>Although GLP-1 therapeutics are currently in the spotlight, Kelly believes the broader lesson is that molecular complexity is increasing across all therapeutic classes.</p><p>This includes:</p><ul><li>Peptides</li><li>Monoclonal antibodies</li><li>Fusion proteins</li><li>Advanced biologics</li></ul><p>Across all these modalities, the need for deeper molecular understanding, early risk detection, and scalable analytical control continues to grow.</p><h4><strong>Biosimilars, Generics, and Equivalence Testing</strong></h4><p>As GLP-1 patents expire, analytical science will play a crucial role in establishing biosimilarity and equivalence.</p><p>Developers must demonstrate rigorous structural and functional comparability between original products and follow-on versions. High-resolution analytical platforms provide the depth of characterization required to prove equivalence while protecting patient safety and therapeutic efficacy.</p><h4><strong>Future Opportunities in Chromatography and Mass Spectrometry</strong></h4><p>Looking ahead, Kelly sees significant innovation opportunities in:</p><ul><li>Greater integration of analytical platforms</li><li>Faster chromatographic separations</li><li>Enhanced multiplexing</li><li>Smarter data analytics</li><li>Increased automation</li><li>Real-time process monitoring</li><li>Process analytical technologies (PAT)</li></ul><p>These developments will bring analytical insights closer to manufacturing environments and improve efficiency across the drug development lifecycle.</p><h4><strong>The Next 5–10 Years</strong></h4><p>Over the next decade, Kelly expects analytical workflows to become:</p><ul><li>More predictive</li><li>More automated</li><li>More interconnected</li><li>Increasingly supported by AI-driven interpretation</li></ul><p>However, she emphasized that these advances must remain grounded in high-quality analytical data generated by ultra-high-resolution instrumentation.</p><h4><strong>Final Thoughts</strong></h4><p>Kelly concluded by highlighting the critical role analytical scientists play in enabling patient access to safe and effective medicines. By identifying hidden risks, characterizing complex molecules, and supporting innovation without compromising scientific rigor, analytical science remains a cornerstone of modern drug development and aligns directly with Thermo Fisher Scientific's mission of making the world healthier, cleaner, and safer.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

How GLP-1 Drugs Are Analyzed: Chromatography & Mass Spectrometry Explained

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Dionex AutoTrace 280 Solid-Phase Extraction Instrument

Extract analytes of interest from large volume aqueous samples easily and efficiently with the Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) Instrument.

<p><strong>Extract analytes of interest from large volume aqueous samples easily and efficiently with the Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) Instrument.&nbsp;</strong></p><p>The AutoTrace 280 automates all four steps of SPE (conditioning, loading, rinsing, and eluting), reducing solvent consumption and improving recovery and reproducibility. Boost productivity and reduce cost of analysis by simultaneously processing up to six samples in 2-3 hours with only 15 minutes of operator involvement. The AutoTrace 280 meets requirements in US EPA methods including the 500 and 600 series.</p><h5><strong>Improved reliability and recovery</strong></h5><ul><li>Automated SPE minimizes operator intervention</li><li>Positive pressure loading and elution of samples and solvents provide constant flow for improved analytical precision</li></ul><h5><strong>Flexibility in operation</strong></h5><ul><li>Loads sample volumes from 20 mL to 4 L</li><li>Compatible with 1, 3, and 6 mL cartridges and with 47 mm SPE disks</li><li>Automatically conditions, rinses, and elutes SPE cartridges with a choice of five solvents</li><li>Offers a choice of six different collection vial racks</li></ul><h5><strong>Improved laboratory safety</strong></h5><ul><li>Minimal sample handling reduces operator exposure to hazardous chemicals</li><li>No hood required</li></ul><h5><strong>Enhanced laboratory productivity and reduced costs</strong></h5><ul><li>Unattended operation</li><li>Batch processing of up to 6 samples</li><li>Reduced solvent usage</li><li>Segregates aqueous and solvent waste using separate waste lines for efficient, cost-effective waste handling</li></ul><h5><strong>Supports a range of matrices and applications</strong></h5><ul><li>Ability to process samples containing suspended solids</li><li>Meets requirements for solid-phase extraction for U.S. EPA methods including 500 and 600 series</li></ul><h5><strong>Recommended use</strong></h5><ul><li>Analyze prepared samples with Thermo Scientific LC or GC systems for a fully integrated workflow</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Instrument Type:</strong> Automated Solid Phase Extraction (SPE) System</li><li><strong>Application:</strong> For use with 47 mm SPE disks</li><li><strong>Model Description:</strong> AutoTrace 280 SPE System</li></ul><h5><strong>Performance &amp; Operating Details</strong></h5><ul><li><strong>Disk Compatibility:</strong> Designed for 47 mm SPE disks</li><li><strong>Pressure Range:</strong><ul><li>Output: 0 to 30 psi (0 to 1.4 bar)</li><li>Input: Up to 100 psi (6.9 bar) maximum</li></ul></li><li><strong>Voltage:</strong> 100–240 V ±10%</li><li><strong>Frequency:</strong> 47–63 Hz</li></ul><h5><strong>Physical Specifications</strong></h5><ul><li><strong>Dimensions (D × W × H):</strong> 57 × 63.5 × 69 cm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific EXTREVA ASE Accelerated Solvent Extractor

Achieve true walkaway sample-to-vial sample preparation with the Thermo Scientific™ EXTREVA™ ASE™ Accelerated Solvent Extractor. 

<p><strong>Achieve true walkaway sample-to-vial sample preparation with the Thermo Scientific™ EXTREVA™ ASE™ Accelerated Solvent Extractor. Without any intervention, perform sample extraction, in-cell cleanup and evaporation—all in one seamless operation—reducing manual labor from hours to minutes and enabling you to focus on other lab priorities while using up to 50% less solvent than other sample preparation techniques.</strong></p><p><strong>Accelerate your journey from sample to vial</strong></p><h5><strong>The EXTREVA ASE Accelerated Solvent Extractor offers:</strong></h5><ul><li><strong>All-in-one sample preparation.</strong> The EXTREVA ASE system automatically extracts and concentrates samples, eliminating the need to manually move sample extracts to another device for solvent evaporation.</li><li><strong>A walk-away solution. </strong>The system minimizes hands-on sample preparation tasks, enabling staff to accomplish other tasks.</li><li><strong>Parallel extraction. </strong>One EXTREVA ASE system can perform four parallel sample extractions, substantially increasing throughput.</li><li><strong>Gas-assisted extraction. </strong>The dynamic gas-assisted extraction mode reduces solvent consumption to between 5 and 100 milliliters per sample in most cases.</li><li><strong>Automation. </strong>Full automation improves recoveries and reproducibility while reducing training needs. Unlike humans, automated systems are ideal for performing the same tedious task repeatedly, and they don’t make mistakes.</li><li><strong>Method flexibility and optimization</strong>. Up to six different solvents can be used, including wash solvents for cleaning between methods. Precise flow control optimizes methods.</li><li><strong>Barcode-reading.</strong> The system tracks sample information and preparation parameters using a 2-D bar code reader to facilitate tracking and integrity. Data is recorded on the instrument or Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Chromeleon™ Chromatography Data System (CDS) software</strong></a>.</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Instrument Type:</strong> Automated Sample Preparation System</li><li><strong>Application:</strong> Simultaneous sample extraction and concentration</li><li><strong>Model Description:</strong> EXTREVA ASE Extraction and Evaporation System</li></ul><h5><strong>Performance &amp; Capabilities</strong></h5><ul><li><strong>Extraction Temperature Range:</strong> 40–200 °C</li><li><strong>Evaporation Temperature Range:</strong> 40–100 °C</li><li><strong>Compatible Solvents:</strong> Wide range of organic and aqueous solvents</li><li><strong>Pressure Capability:</strong> Up to 200 psi</li><li><strong>Power Consumption:</strong> 2000 W</li><li><strong>Frequency:</strong> 50/60 Hz</li></ul><h5><strong>Sample &amp; Solvent Handling</strong></h5><ul><li><strong>Extraction Cells (stainless steel):</strong> 1, 5, 10, 22, 34, 66, 100 mL</li><li><strong>Extraction Cell Tray:</strong> 16-position with 1 rinse</li><li><strong>Collection Vials:</strong><ul><li>60 mL and 100 mL evaporation vials</li><li>250 mL evaporation bottles</li><li>Direct concentrate to 2 mL sample vials with rinse capability</li></ul></li><li><strong>Collection Vial Tray:</strong> 16-position tray insert for 60 mL vials and 250 mL bottles; includes 4 rinse positions</li></ul><h5><strong>Utilities &amp; Requirements</strong></h5><ul><li><strong>Pneumatic Requirements:</strong><ul><li>Air: 130–140 psi</li><li>Nitrogen: 350–450 psi</li></ul></li></ul><h5><strong>Physical Specifications</strong></h5><ul><li><strong>Dimensions (D × W × H):</strong> 65.2 × 100 × 88.6 cm (25.6 × 39.4 × 34.8 in.)</li><li><strong>Weight:</strong> 160 kg (353 lb)</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor

Save time, reduce solvent consumption, and boost productivity with Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor.

<div><p><strong>Automate extraction, filtration and clean up of compounds from solid and semisolid samples in minutes using the automated Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor. This system accommodates sample sizes of 1-100g, allows unattended extraction of up to 24 samples and uses 50 to 90% less solvent compared to other methods.&nbsp;</strong></p><p>The chemically inert pathway supports acid and alkaline sample matrices and solvents. Flexible, easy-to-operate and cost-effective, the Dionex ASE 350 is ideally suited for high-throughput laboratories and for a wide variety of applications.</p><div><h4><strong>Rapid and efficient extractions</strong></h4><ul><li>Elevated temperature (up to 200°C) and pressure (1500psi) reduce extraction times and solvent consumption</li><li>Fast pump (70mL/min.) enables fast extractions (typically 12-20 minutes)</li><li>Specialized oven design ensures precise temperature control for excellent reproducibility</li><li>Integrated solvent controller allows mixing and delivery of up to three solvents</li><li>The <strong>Thermo Scientific™ SmartRun™ System</strong> ensures cell size and collection vessel size are matched for an optimal extraction</li></ul><h4><strong>Enhanced flexibility to support multiple applications</strong></h4><ul><li>System accommodates <strong>1, 5, 10, 22, 34, 66 </strong>and <strong>100mL cell sizes</strong></li><li>pH-hardened pathway with <strong>Thermo Scientific™ Dionium™ components and cell</strong> enables compatibility with acid or alkaline pretreated matrices</li></ul><h4><strong>Dramatically reduced solvent consumption</strong></h4><ul><li>Requires less than 50mL solvent for a 20g sample</li><li>Includes solvent saver mode for further solvent savings</li></ul><h4><strong>Enhanced laboratory productivity</strong></h4><ul><li>System holds up to 24 samples for unattended operation</li><li>Flow-through technology allows in-line filtration and in-cell cleanup</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_ASE_350_system_2_9426ff3f9a/Thermo-Scientific-Dionex-ASE-350-system_2.jpg" alt="Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_ASE_350_system_2_9426ff3f9a/thumbnail_Thermo-Scientific-Dionex-ASE-350-system_2.jpg 114w," sizes="100vw" width="579" height="792"></p><h3><strong>🛠️ Specifications</strong></h3><ul><li><strong>Collection Vial Tray</strong>: 26-position tray insert for 60 mL vials; 19-position tray insert for 250 mL bottles</li><li><strong>Depth (English)</strong>: 27.3 in.</li><li><strong>Depth (Metric)</strong>: 69.3 cm</li><li><strong>Description</strong>: Extractor Only</li><li><strong>Extraction Fluids</strong>: Compatible with a wide range of organic and aqueous solvents</li><li><strong>Fluid Sensors</strong>: IR</li><li><strong>Frequency</strong>: 50/60 Hz</li><li><strong>Height (English)</strong>: 24.3 in.</li><li><strong>Height (Metric)</strong>: 61.7 cm</li><li><strong>Pneumatic Requirements</strong>: Air at 400–827 kPa (60–120 psi); N2 (at 1034 ×–1340 kPa (150–200 psi))</li><li><strong>Power Consumption</strong>: 500 Max VA</li><li><strong>Pressure</strong>: 10 MPa (1500 psi)</li><li><strong>Pump Flow</strong>: 70 mL/min</li><li><strong>Temperature Control</strong>: Up to 200 °C</li><li><strong>Weight (English)</strong>: 140 lb.</li><li><strong>Weight (Metric)</strong>: 65 kg</li><li><strong>Width (English)</strong>: 26.5 in.</li><li><strong>Width (Metric)</strong>: 67.3 cm</li><li><strong>Collection Vials</strong>: 60, 250 mL</li><li><strong>Dimensions (D x W x H)</strong>: 69.3 x 67.3 x 61.7 cm</li><li><strong>Extraction Cell Tray</strong>: 24 Cell, 2 Rinse</li><li><strong>Extraction Cells</strong>: 1, 5, 10, 22, 34, 66, 100 mL</li><li><strong>For Use With (Equipment)</strong>: Dionex ASE 350</li><li><strong>Type</strong>: Dionex ASE 350 System</li><li><strong>Unit Size</strong>: Each</li></ul></div></div>

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