<div><div><div><div><h3><strong>How Does Your Monthly Nitrogen Gas Bill Compared to Other Labs?</strong></h3><p><span><strong>If your lab relies on nitrogen gas cylinders, you already know how quickly costs can add up. Between </strong></span><span style="font-weight:normal;"><strong>purchase prices, rental fees, delivery charges, and frequent refills</strong></span><span><strong>, </strong></span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/understanding-the-costs-of-renting-nitrogen-gas-cylinders-in-laboratories?hsLang=en"><span><strong>nitrogen cylinders can become a </strong></span><span style="font-weight:normal;"><strong>hidden financial drain</strong></span></a><span><strong> on your operations.</strong></span></p><p><span>But how do you know if you're paying a fair price—or if you're overspending compared to other labs? Our </span><i><span style="font-weight:normal;">"Am I Overpaying on my Nitrogen Gas Cylinders?"</span></i><span> tool helps you determine whether your current nitrogen supply costs are within an acceptable range or if you're paying too much.</span></p><h3><strong>The Hidden Costs of Nitrogen Cylinders</strong></h3><p><span>Nitrogen cylinders are a staple in many laboratories, but they come with several costly drawbacks:</span></p><h4><strong>1. High Cylinder Costs</strong></h4><p><span>The upfront cost of nitrogen cylinders can be significant, especially for labs that require multiple cylinders per month. Pricing varies depending on size and supplier, but the recurring need to purchase or replace cylinders creates an ongoing expense that can quickly add up. Unlike a fixed investment in a nitrogen generator, cylinder costs are a </span><span style="font-weight:normal;">constant and unavoidable</span><span> part of lab operations.</span></p><h4><strong>2. Monthly Rental Fees</strong></h4><p><span>Most gas suppliers charge a rental fee per cylinder, adding to the overall cost of maintaining a steady nitrogen supply. These fees might seem small at first, but when multiplied across multiple cylinders and months, they become a substantial recurring expense. Over time, these rental costs can easily surpass the price of investing in an in-house nitrogen generation system.</span></p><h4><strong>3. Delivery &amp; Handling Expenses</strong></h4><p><span>Delivery costs vary but can </span><span style="font-weight:normal;">add hundreds of dollars per month</span><span> to your nitrogen expenses, especially if you're receiving frequent shipments. Beyond costs, frequent deliveries create </span><span style="font-weight:normal;">workflow disruptions </span><span>and </span><span style="font-weight:normal;">safety concerns</span><span> due to cylinder handling and storage. Each delivery requires time, coordination, and often additional fees, making cylinders an inefficient option for labs that require a reliable nitrogen supply.</span></p><h4><strong>4. Gas Waste</strong></h4><p><span>Did you know that most nitrogen cylinders leave </span><span style="font-weight:normal;">about 10% of their gas unused </span><span>due to pressure limitations? That means you're </span><span style="font-weight:normal;">paying for nitrogen you can’t even use</span><span>. Additionally, each time a cylinder runs empty, there is downtime involved in switching to a new one, causing further inefficiencies in lab operations.</span></p><h4><strong>5. Storage &amp; Safety Risks</strong></h4><p><span>Cylinders require dedicated storage space and proper handling to </span><span style="font-weight:normal;">prevent leaks, pressure hazards, and accidents</span><span>. Many labs struggle with cylinder management, leading to </span><span style="font-weight:normal;">compliance concerns and workflow inefficiencies</span><span>. Storing multiple high-pressure cylinders also introduces potential safety risks, requiring strict adherence to safety protocols and regulatory standards.</span></p><h3><strong>The Smarter Alternative: Switching to a Nitrogen Generator</strong></h3><p><span>A </span><span style="font-weight:normal;">nitrogen generator</span><span> offers a </span><span style="font-weight:normal;">cost-effective, on-demand</span><span> solution for labs that need a steady nitrogen supply—without the ongoing expenses of cylinder rentals, refills, and deliveries. Here’s why more labs are making the switch:</span></p><ul><li><span><strong>Eliminate Cylinder Costs</strong> – No more purchases, rentals, or refills.</span></li><li><span><strong>Reduce Long-Term Expenses</strong> – A one-time investment with minimal maintenance.</span></li><li><span><strong>On-Demand Supply</strong> – Generate </span><span style="font-weight:normal;">high-purity nitrogen</span><span> whenever you need it.</span></li><li><span><strong>No More Deliveries</strong> – Say goodbye to disruptions and recurring delivery fees.</span></li><li><span><strong>Increased Safety &amp; Efficiency</strong> – No handling of high-pressure cylinders.</span></li><li><span><strong>Better Environmental Impact</strong> – </span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/are-nitrogen-generators-actually-more-environmentally-friendly?hsLang=en"><span>Reduce carbon emissions</span></a><span> associated with cylinder deliveries and production.</span></li></ul><p><span>Many labs find that a </span><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/would-a-nitrogen-generator-make-sample-preparation-more-affordable?hsLang=en"><span style="font-weight:normal;">nitrogen generator pays for itself within a year</span></a><span>—especially if they use multiple cylinders per month. Beyond the financial savings, labs that </span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/why-nitrogen-generators-are-gaining-popularity?hsLang=en"><span>switch to a nitrogen generator</span></a><span> experience </span><span style="font-weight:normal;">greater efficiency, safety, and reliability</span><span> in their daily operations.</span></p><blockquote><p><span><strong>Learn More: </strong></span><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/nitrogen-generators-for-sample-preparation-everything-you-need-to-know?hsLang=en"><span><strong>Nitrogen Generators for Sample Preparation: Everything you need to know</strong></span></a></p><p><span><strong>Watch Our Webinar: </strong></span><a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/the-case-for-nitrogen-generation-in-your-lab?hsLang=en"><span><strong>Improving Laboratory Workflows with Nitrogen Generation</strong></span></a></p></blockquote><h3><strong>Find Out If You’re Overpaying</strong></h3><p><span>Not sure if switching to a nitrogen generator makes sense for your lab? Use our </span><i><span style="font-weight:normal;">"Am I Overpaying on My Nitrogen Gas Cylinders?"</span></i><span> tool to compare your costs against industry benchmarks. Simply answer a few quick questions, and we’ll help you determine whether it’s time to make the switch.</span></p></div></div></div></div>

Am I Paying Too Much for my Nitrogen Gas Cylinders?

<p><strong>Intensive farming, even when good agricultural practices are followed, very often results in pesticide contamination of soil, surface water and groundwater. Water sources used for drinking water production are thus often affected. The outcome is the progressive banning of proven, effective, yet persistent pesticides in the environment, alongside a decline in water quality. This, in turn, results in substantial investments in water treatment technologies to ensure that drinking water meets relevant legal standards. Passive, easy to maintain and low-cost in-situ denitrification woodchip bioreactors might be a solution to this situation. These bioreactors have already been successfully used in some countries (e.g. the USA) to remove other types of agricultural pollution directly at the point of origin, thus preventing its further transport in the environment.&nbsp;</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_ba53628b5a.jpg" alt="ALS Czech Republic: EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape: Figure 1: A real example of an installed semi-operational bioreactor unit." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_ba53628b5a.jpg 245w," sizes="100vw" width="1971" height="919"></p><h3><strong>Nitrates and Denitrifying Bioreactors</strong></h3><p>Denitrifying woodchip bioreactors are a relatively simple containerised treatment technology originally intended for the removal of nitrates from agricultural outflows. They use woodchip media as a source of bioavailable organic carbon to promote denitrification, which converts nitrates to gaseous nitrogen, the predominant natural component of the atmosphere. The advantages of using denitrification woodchip bioreactors are low acquisition and operating costs, minimal maintenance, long lifetime, and low land take. At some sites, these bioreactors have been operated for more than 15 years without major interventions and with the original fill and still achieve high denitrification rates.</p><h3><strong>ALS Research Project</strong></h3><p>To verify the usability of denitrifying woodchip bioreactors for the simultaneous removal of both nitrates and pesticides found in surface runoff from agriculturally active areas is the goal of research project "Comprehensive assessment of soil contamination with pesticides and in-situ remediation leading to eliminate their entry into groundwater“. The project is being realised under the leadership of ALS Laboratories in cooperation with other research institutes. Work on the project will continue until 2025.</p><h3><strong>Semi-operational Unit and Expected Pesticides Removal</strong></h3><p>Previous research projects by ALS and their partners have thoroughly mapped and identified sites that have been, in terms of pesticides occurrence, problematic for a long time. A semi-operational unit of the denitrification woodchip bioreactor described above was commissioned at one of these sites in the autumn of 2023.</p><p>The unit uses poplar wood chips as a feedstock, which have proven well for this purpose, and is equipped with automatic samplers, sensors measuring basic physical and chemical parameters and a data station with on-line data transmission.</p><p>In the case of pesticides, several decontamination mechanisms are envisaged in the bioreactor area, the most important of which will be the sorption and microbial decomposition. The aim of the project is to suggest the operating conditions of the bioreactor in such a way that the natural processes of microbial degradation of pesticides are facilitated to the maximum extent possible, or to support these processes by active intervention in the physicochemical composition of the bioreactor and its microflora.</p><blockquote><p><strong>Related EnviroMail_08_Europe:</strong><br><a target="_blank" rel="noopener noreferrer" href="https://www.alsglobal.eu/enviromails/enviromail_08"><strong>Hidden Pesticide Threats in Protected Landscape Areas</strong></a></p></blockquote><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_2_4e7e587035.jpg" alt="ALS Czech Republic: EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape: Figure 2: Results of pesticides detected in tested soil samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_17_Europe_Protecting_Drinking_Water_Sources_with_Bioreactors_An_Innovative_Solution_to_the_Problem_of_Pesticides_in_the_Landscape_2_4e7e587035.jpg 245w," sizes="100vw" width="1439" height="783"></p><h3><strong>Case Study: Monitoring of Pesticides in Soil Sam- ples from Central Europe</strong></h3><p>In June 2023, monitoring of pesticides in agricultural soils and surrounding surface waters was conducted from 45 sites across Central Europe. The range of determined targeted substances was based on prepared risk analysis taking into account the consumption of pesticides and their risk properties such as toxicity and persistence. Analytical methods based on liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were used to determine a wide range of active pesticide substances and their metabolites (308 and 352 parameters, respectively). Results are summarized in <strong>Figure 2</strong>.</p><p>Pesticides were detected in all collected soil samples ranging from 0.01 to 0.73 mg/kg DW (dry weight of soil). By far the most abundant group was glyphosate pesticides, i.e. the herbicide glyphosate and especially its metabolite AMPA (Aminomethylphosponic acid), its total concentrations ranged from 0.01 to 0.46 mg/ kg DW in 36 of the 45 samples collected and tested. Comparing to levels and incidence of pesticides within the Europe, glyphosate and AMPA belong to the most frequently detected and those with the highest concentrations pesticide compounds generally.</p><p>In related surface water samples, pesticides were detected in 43 out of 45 tested samples, their concentrations ranged from 0.01 μg/L to 14.1 μg/L. The most abundant pesticides detected were chloracetanilide and glyphosate.</p><h3><strong>Protective Measures in the Landscape</strong></h3><p>Based on the results obtained during the testing of the semi-operational denitrification woodchip bioreactor unit, a linear measure will be designed and properly sized for the model catchment to remove pesticides directly at the point of generation and thus prevent their further spread.</p><h3><strong>European Legislation</strong></h3><ul><li><strong>SOIL:</strong> Proposal COM(2023) 416 final of 5 July 2023 for a directive of the European Parliament and of the Council on Soil Monitoring and Resilience (Soil Monitoring Law).</li><li><strong>SURFACE WATER:</strong> Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on environmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 2000/60/EC of the European Parliament and of the Council.</li><li><strong>GROUND WATER: </strong>Directive 2006/118/EC of the European Parliament and of the Council of 12 December 2006 on the protection of groundwater against pollution and deterioration.</li><li><strong>DRINKING WATER: </strong>Directive (EU) 2020/2184 of the European Parliament and of the Council of 16 December 2020 on the quality of water intended for human consumption (recast).</li></ul>

EnviroMail™ 17/Europe: Protecting Drinking Water Sources with Bioreactors: An Innovative Solution to the Problem of Pesticides in the Landscape

<p><strong>Mushrooms – a fragrant forest treasure for some, a fascinating object of study for others. In this case, the connection between nature and science is entirely natural. What can you discover about a simple mushroom under a microscope or using chromatography? And how helpful is a scientific education when it comes to everyday mushroom hunting?&nbsp;</strong></p><p>We asked <strong>RNDr. Roman Kysilka, CSc.</strong>, who worked as a research specialist at the Czechoslovak Academy of Sciences and now works at <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"><strong>Watrex Prague</strong></a>. He walked us through not only his career, but also through the forest – both scientific and real.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_19_6_uvod_1600_X900_4dca2a04b3.jpg" alt="Watrex Fig.1: RNDr. Roman Kysilka, CSc." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_19_6_uvod_1600_X900_4dca2a04b3.jpg 245w," sizes="100vw" width="3540" height="1875"></p><h4><strong>When and why did you decide to study natural sciences? Were you drawn to them as a child?</strong></h4><p>I got a chemistry experiment kit, I think it was called "Curious Chemist," around seventh grade, and I was immediately captivated by home experiments. I was also into electricity and experimented with that as well. In my early years of high school, I was still considering studying electrical engineering or chemistry. But chemistry won out, largely thanks to the chemistry olympiads I took part in regularly from elementary school. Through national competitions (later in high school), I met a lot of chemistry enthusiasts. And during training camps for the international olympiad, I got to know chemists from the Faculty of Science at Charles University – and that sealed the deal.</p><h4><strong>How did you first get into studying mushrooms? Was it by chance or intentional?</strong></h4><p>It was a coincidence. I did grow champignons when I was around twelve, but that had nothing to do with research – I was mostly curious about what my mom would cook with them. Between 1982 and 1984, I was working on my thesis, and my advisor, Prof. Karel Štulík, arranged for me to work in a chromatography lab in the Department of Experimental Mycology at the Institute of Microbiology of the Czechoslovak Academy of Sciences. And that’s where I first encountered mushrooms as a subject of scientific research.&nbsp;</p><h4><strong>What fascinated you most about mushrooms?&nbsp;</strong></h4><p>Mushrooms have been with humankind since ancient times. You might recall the phrase “mushrooms – meat of the poor.” The Aztecs used the word teonanácatl, which roughly translates as “meat of the gods” (later found to refer to mushrooms with psychoactive effects). Meat for both the poor and the gods – interesting, right? The diverse world of fungi – heterotrophic organisms with a vital role in nature – fascinated me from the start. And of course, there’s also the unexpected potential of the compounds mushrooms produce. Every year, hundreds of new, previously unknown chemical compounds – secondary metabolites – are isolated and described in scientific literature, with potential uses in medicine and other fields. You could say the world of fungi is still largely unexplored from a chemical standpoint.&nbsp;</p><h4><strong>You worked at the Czechoslovak Academy of Sciences from 1985–1991 – what exactly did you do there?&nbsp;</strong></h4><p>After returning from military service, I went back to the Institute of Microbiology, where I focused on research into psychoactive tryptamine derivatives in basidiomycetes for my dissertation. The list of substances later narrowed to psilocybin, psilocin, baeocystin, and bufotenin. I worked on developing analytical methods to detect these compounds in fruiting bodies and mycelium, designing chromatographic methods for semi-preparative scale isolation, conducting cultivation experiments with various Psilocybe species in the lab, and screening potentially psychoactive mushrooms for these compounds.&nbsp;</p><h4><strong>Do you recall a moment or discovery that left a strong impression on you?&nbsp;</strong></h4><p>Yes. Part of the research on magic mushrooms included experimental poisonings. In the late 1980s, together with Marta Semerdžieva, we completed (and later patented) a method for producing mushroom mycelium via submerged cultivation. We planned an intoxication experiment using this rather unappealing material. Based on HPLC analysis, we cooked a “mushroom” soup and began the experiment. However, the outcome was very different from what we expected – it was clear we had significantly overdosed the test subjects. That prompted a detailed review of the analytical method used to determine psilocybin and psilocin levels. We found that the critical step for accurate results was the sample preparation before HPLC analysis. With optimized extraction, we detected concentrations of active compounds several times higher than with previously published methods. Our article in Planta Medica is still cited today and occasionally discussed in forums about psychoactive substances.&nbsp;</p><h4><strong>What techniques were you using then – and what surprises you most about modern lab methods?&nbsp;</strong></h4><p>Our main analytical technique was HPLC. Mass spectrometry, which is standard today, was just a dream back then. The peak was using a UV-VIS diode-array detector or selective and sensitive amperometric detection. I find the current development of lab automation, the explosive growth of computing and mathematical modeling, and more recently, the use of artificial intelligence truly remarkable.</p><h4><strong>How did you transition from academia to Watrex Prague? What attracted you to the change?</strong></h4><p>In the early '90s, research projects at the Institute of Microbiology were being reorganized, and several colleagues I worked with left the institute. Around the same time, I got a job offer from Milan Minárik, who was founding <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"><strong>Watrex</strong></a> – a distributor for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/325"><strong>Waters</strong></a> chromatography systems. I had met Milan a few years earlier (through HPLC, of course), and the idea of working as an applications specialist in his private company was very appealing. It was a time of rapid private sector growth in Czechoslovakia, and I was excited to be a part of that.&nbsp;</p><h4><strong>You’ve studied mushrooms scientifically, but what about in the actual forest? Are you a mushroom picker?</strong></h4><p>I love picking mushrooms (their fruiting bodies), but I don’t have as much time for it as I’d like.&nbsp;</p><h4><strong>How much do you rely on your scientific knowledge when mushroom picking? Do you see mushrooms differently than the average picker?&nbsp;</strong></h4><p>Rather than scientific knowledge, I rely on experience from field excursions with the Institute of Microbiology, where I had the chance to learn mushroom identification from leading Czech mycologists. And unlike “typical pickers,” I often collect gilled mushrooms. My three-year-old granddaughter Anička might actually be able to name more bolete species than I can.</p><h4><strong>Does your knowledge of chemistry help with identifying lesser-known species?</strong></h4><p>No.&nbsp;</p><h4><strong>Do you have a favorite mushroom – either for eating or just because it’s interesting?&nbsp;</strong></h4><p>Aside from magic mushrooms, I have a soft spot for the fly agaric. Amanita muscaria, with its unmistakable red cap and white spots, has a rich and ancient history. Its story intersects with folk medicine and shamanism across many cultures, especially in the Northern Hemisphere. Another mushroom worth mentioning is the deadly Cortinarius orellanus. It grows in our region and can unfortunately be confused with some edible species. It contains the nephrotoxin orellanine, which is dangerous due to its delayed onset – symptoms can appear up to three weeks after ingestion, which makes treating the poisoning extremely difficult.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_19_6_obr_2_02794e1777.jpg" alt="Watrex Fig.2: Illustrative photo of Amanita muscaria and Cortinarius orellanus" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_19_6_obr_2_02794e1777.jpg 245w," sizes="100vw" width="1600" height="839"></p><h4><strong>What can we learn about a mushroom in a laboratory — apart from whether it’s edible or not?&nbsp;</strong></h4><p>A mushroom, as a subject of laboratory study, can be analyzed taxonomically; we can identify substances that are toxicologically significant, search for new biologically active compounds, or use fungi as model organisms in genetic research. Their bioaccumulation capacity can help monitor unwanted substances in the environment. And I’m sure there are many other possibilities for scientific investigation in the lab.&nbsp;</p><h4><strong>Have you ever examined the presence of toxins, heavy metals, or pesticides? How common are they in mushrooms?&nbsp;</strong></h4><p>The ability of mushrooms to accumulate foreign substances is well documented and stems from the way they absorb nutrients from their surroundings. Their mycelium has a huge surface area and absorbs not only nutrients from the soil solution, but also unwanted substances, which may then accumulate in the fruiting bodies. I personally haven’t focused on bioaccumulation research in fungi — with one exception. After the Chernobyl nuclear disaster, we collected various mushroom species to monitor levels of Cesium-137.&nbsp;</p><h4><strong>What’s your opinion on identifying mushrooms using mobile apps?&nbsp;</strong></h4><p>If a mobile app serves as a replacement for a quality, well-illustrated mushroom atlas, I have no objections. However, I’d be very cautious when using various "identification keys." An amateur can easily overlook or misinterpret an important feature that differentiates an edible mushroom from an inedible or even poisonous one. That said, with the advancement of AI, things will surely improve :). As always — only collect mushrooms you can identify with absolute confidence!&nbsp;</p><h4><strong>What’s something every mushroom hunter should know — but often overlooks?</strong></h4><p>Where north is — and how to get back to the train station or their parked car.</p><h4><strong>Besides a knife, is there anything else you’d bring in your mushroom basket?&nbsp;</strong></h4><p>A camera. Mushrooms are incredibly photogenic. And if one doesn't end up in the basket, it can at least become a model for photography.&nbsp;</p><h4><strong>And finally: If you had to describe mushrooms in one word, what would it be?</strong></h4><p>Saprophytes. Without them, life on our planet would come to a halt.&nbsp;</p><p><i><strong>Many thanks to Roman Kysilka for such an open and inspiring conversation. It was a pleasure to explore his professional journey and the unique experiences that have shaped it. Roman currently works at </strong></i><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky"><i><strong>Watrex Praha</strong></i></a><i><strong>, where his expertise and experience continue to make a valuable impact. We wish him all the best in his future professional and personal endeavors!</strong></i></p>

Are you scientifically prepared for the new mushroom-picking season?

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<h4><strong>An experienced partner in the LC-MS/MS analysis industry</strong></h4><p>If you are interested in performing quantitative, qualitative or mixed analysis using LC-MS/MS analytical methods but lack such capabilities due to lack of equipment, “know-how” or are looking for a team to perform analysis for a project - you have come to the right place. We work on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315"><strong>equipment</strong></a> with high sensitivity, speed and specificity. Our team is a group of specialists with comprehensive experience in the industry. The LabExperts <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/zespol/">team</a> has participated in many scientific projects, R&amp;D and commercial implementations. We take an individual and factual approach to developing a new method and performing analyses:</p><ul><li>In the first stage, we get to know your requirements - to align expectations with capabilities;</li><li>Next, we will form and approve the framework for cooperation, ensuring the observance of confidentiality by signing a non-disclosure agreement (NDA);</li><li>We will plan together and, for our part, meet the deadlines for the completion of the set tasks;</li><li>We will perform preliminary studies, after which we will decide together whether and to what extent we continue to develop the methods;</li><li>We will handle the development of the sample preparation procedure;</li><li>We will select the best parameters for the LC-MS/MS analytical method;</li><li>The analytical method along with the sample preparation procedure will be validated according to generally accepted guidelines and standards, or we will establish the required acceptance criteria and ranges for your needs;</li><li>We will perform LC-MS/MS analyses of a series of samples;</li><li>We will provide results in the form of easy-to-read reports at each required stage;</li><li>We will evaluate the feasibility and assist in the transfer of the method to your laboratory.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg" alt="Bioanalytic: Bioanalytic Development of LC-MS-MS method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg 234w," sizes="100vw" width="1536" height="1024"></p><p>Pricing and the framework for cooperation are always determined individually depending on the scope of the study. This is influenced by the amount of work and the complexity of the development of the sample preparation procedure and analytical method and routine analytics. As a rule, the final price consists of three separately priced elements: the cost of preliminary studies, method validation, and the cost of analysis of one sample within a series of analyses.</p><p>Don't beat your head against the wall - we will help you achieve your goal!</p><p>We look forward to working with you!</p>

Bioanalytic Individual LC-MS/MS training

We are ready to prepare customized LC-MS/MS training for the needs of participants.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Customized LC-MS/MS Training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong> or other</li><li>TRAINING TIME: <strong>1-5 days</strong> or more</li><li>NUMBER OF PARTICIPANTS: <strong>4-8 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP/QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg" alt="Bioanalytic: Bioanalytic Individual LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg 245w," sizes="100vw" width="1536" height="930"></p><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Customized LC-MS/MS training we are ready to prepare for the needs of participants, discussing:</p><ul><li>theoretical basics of chromatography, mass spectrometry,</li><li>practical operation of the LC-MS/MS system,</li><li>qualitative and quantitative analysis,</li><li>DDA &amp; DIA screening methods,</li><li>“omics” analyses,</li><li>validation of analytical methods,</li><li>solving basic methodological and technical problems,</li><li>software,</li><li>calibration,</li><li>“tips&amp;tricks”,</li><li>etc...</li></ul><p>We offer training for both novices starting the fascinating adventure of LC-MS/MS analytics, as well as for those who are LC-MS/MS users and are interested in expanding and consolidating their knowledge.</p><h4><strong>Framework training program</strong></h4><ul><li>individual LC-MS/MS training is developed according to the customer's demand</li><li>conducting theoretical and/or practical training at the customer's workplace</li><li>carrying out theoretical and/or practical training in a laboratory in Lodz</li></ul>

Bioanalytic Basic LC-MS/MS training

Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Basic LC-MS/MS training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP </strong><span class="ts-pf-details-content"><strong>&amp; </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg" alt="Bioanalytic: Bioanalytic Basic LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg 205w," sizes="100vw" width="1293" height="987"></p><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument. Basic LC-MS/MS training is primarily aimed at those who are new to LC-MS/MS equipment or who wish to become familiar with this analytical technique. During the training, the following will be presented:</p><ul><li>principles of developing and optimizing quantitative and qualitative methods on low (triple quadrupole, QTRAP) and high resolution (QTOF) instruments,</li><li>basic functionality of the software,</li><li>procedures and objectives of instrument calibration,</li><li>principles of maintaining the cleanliness of mass spectrometer operation.</li></ul><h5><strong>Basic LC-MS/MS training - the program</strong></h5><p><span style="font-size:20px;"><strong>Day I</strong></span></p><p><strong>Theoretical classes:</strong></p><ul><li>Introduction to LC</li><li>Introduction to mass spectrometry (basics)</li><li>Quantitative analysis.</li></ul><p><strong>Practical part:</strong></p><p>QTRAP:</p><ul><li>QTRAP scanning modes on the instrument - Q1, , MS2, MRM, demonstration and discussion on the example of a selected pesticide of the fragmentation spectrum and its fragmentation pathway.</li><li>MRM pair formation (manual, semi-automatic, automatic).</li><li>optimization of ion source parameters -T-conector</li><li>explanation of the main parameters related to LC (column selection, gradient vs isocrat, autosampler)</li><li>LC-MS/MS method setup and optimization</li></ul><p>QTOF:</p><ul><li>manual and automatic optimization of MRM<sup>HR</sup></li><li>ion source and LC parameters (transfer from method developed above)</li></ul><p>Sequence of quantitative analysis on both instruments.</p><p><span style="font-size:20px;"><strong>Day II</strong></span></p><p><strong>Quantitative analysis:</strong></p><ul><li>data processing and analysis,</li><li>reporting - Sciex OS (QTRAP, QTOF).</li><li>Discussion of types of integration algorithms (MQ4, autopeak) and integration parameters.</li><li>independent selection of integration parameters and plotting of master curves.</li><li>independent data analysis and generation of analysis report.</li></ul><p><strong>Qualitative analysis:</strong></p><ul><li>Theoretical introduction - introduction to qualitative analysis:</li><li>qwadrupole modes (Prec/NL),</li><li>trap modes (EMS, EPI, MS3),</li><li>QTOF modes (TOF MS, TOF MS/MS).</li><li>mixed methods,</li><li>targeted vs. untargeted analysis,</li><li>DDA (IDA) - discussion of method development,</li></ul><p><strong>Practical part:</strong></p><ul><li>creation of qualitative methods on QTRAP (Prec-IDA, MRM-IDA), other parameters (LC, ion source) as the previous day,</li><li>LightSight - discussion of operation, creation of MRM-IDA multimethod for selected pesticide and analysis of samples (night).</li><li>QTOF - creation of IDA method, other parameters (LC, ion source) as the previous day.</li></ul><p>Sequence of qualitative analysis overnight.</p><p><span style="font-size:20px;"><strong>Day III</strong></span></p><p><strong>Qualitative data analysis:</strong></p><p>QTRAP:</p><ul><li>Discussion of Prec-IDA, MRM-IDA results - what we see in different types of MS and MS/MS scans. Manual review of data and via IDA Explorer (SciexOS).</li><li>Analysis of results in LightSight, comparison with Prec-IDA, for example - advantages/disadvantages</li></ul><p>QTOF:</p><ul><li>search for potentially present compounds in the sample,</li><li>manual comparison of blank vs. test sample,</li><li>manual by XIC extraction (by sum formula),</li><li>Formula finder - derivation of sum formula on TOF MS data and verification of fragmentation on TOF MS/MS data</li><li>search in IDA Explorer mode - application of filters: similarity, isotopic distribution, mass defect,</li><li>database searching,</li><li>Molecule Profiler - demonstration of software capabilities on selected pesticide example,</li></ul><p><strong>Instrument calibration:</strong></p><ul><li>resolution, mass-to-charge ratio, intensity,</li><li>Q1 and Q3, LIT (MS and MS/MS), TOF MS and TOF-MS/MS</li><li>Automation vs. manual calibration,</li></ul><p><strong>LC-MS/MS application examples</strong></p><p><strong>Summary</strong></p><p><strong>Discussion</strong></p>

Bioanalytic Quantitative LC-MS/MS analysis training

The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Quantitative LC-MS/MS analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP LC-MS/MS</strong></li></ul><figure class="image"><img style="aspect-ratio:1200/675;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg" alt="Bioanalytic: Bioanalytic Quantitative LC-MS-MS analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg 245w," sizes="100vw" width="1200" height="675"></figure><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Quantitative LC-MS/MS analysis - the training is designed for laboratory personnel using LC-MS/MS systems for quantitative analysis. The following topics are covered during the training:</p><ul><li>Triple quadrupole spectrometer calibration</li><li>MRM pair formation and optimization</li><li>Optimization of ion source parameters</li><li>Optimization of chromatographic parameters</li><li>Sample preparation using the example of blood drawn on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1283"><strong>Mitra®</strong></a> samplers (VAMS)</li><li>Matrix effect, internal standards (ISTD), matrix curves</li><li>Validation of the quantitative method</li><li>Reporting of results</li></ul><p>The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis while covering the widest possible linear range of concentrations.</p><h3><strong>Training program</strong></h3><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part:</strong></h5><p>Triple quadrupole structure, ionization, MRM and sMRM scanning, chromatography, multimethods</p><h5><strong>Practical part:</strong></h5><ul><li>Q1/Q3 calibration (10 Da/s), MS/MS - resolution, m/z, intensity.</li><li>MRM pair formation and optimization (manual, semi-automatic, automatic).</li><li>Ion source optimization - T-concector and FIA</li><li>Gradient vs isocrat, dosing, column selection, temperature selection, other important parameters</li><li>Establishment and optimization of LC-MS/MS method.</li><li>How to take care of MS cleanliness - ionization time programming, multi-way valve, cleaning of ion source and instrument front end</li></ul><p>Sample preparation in pure solvent (standard curve, QC, solvent, solvent+ISTD, test samples), overnight analysis sequence.</p><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><h5><strong>Practical part - preparation of whole blood samples (Mitra®):</strong></h5><ul><li>Preparation of matrix standard curves</li><li>Preparation of quality control (QC) samples in three concentrations</li><li>Preparation of blank samples (without ISTD and with ISTD).</li><li>Preparation of samples for recovery determination (“pre-spiked” and “post-spiked”)</li><li>Preparation of test samples</li></ul><h5><strong>Theoretical part:</strong></h5><ul><li>Samples in the matrix - discussion of the matrix effect and ways to level it.</li><li>Optimization of sample preparation method and LC-MS/MS method</li></ul><h5><strong>Practical part:</strong></h5><ul><li>Sample extraction from Mitra® samplers for LC-MS/MS analysis.</li><li>Sequence of analysis of prepared samples overnight</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><h5><strong>Theoretical part:</strong></h5><ul><li>Validation of the quantitative LC-MS/MS method</li></ul><h5><strong>Practical part:</strong></h5><ul><li>Software - data integration algorithms, signal smoothing, weighing.</li><li>Matrix curves vs curves in solvent, influence of ISTD (Sciex OS).</li><li>Method validation - validation criteria, determination of LOD, LLOQ, ULOQ, linearity, working range of the method, repeatability, recovery.</li><li>Preparation of summary validation reports</li><li>Reporting of results from test samples - report vs export of data to Excel.</li><li>Summary and discussion.</li></ul>

Bioanalytic QTRAP vs QTOF Quality analysis training

The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>QTRAP vs QTOF Quality analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP </strong><span class="ts-pf-details-content"><strong>&amp; </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg" alt="Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg 221w," sizes="100vw" width="1000" height="707"></p><h4><strong>TRAINING COURSE DESCRIPTION</strong></h4><p><strong>QTRAP vs QTOF qualitative analysis</strong> - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of <strong>DDA (IDA)</strong> (QTRAP &amp; QTOF) and <strong>DIA (SWATH) </strong>(QTOF) methods. Analysis and comparison of results will be conducted using <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/sciex-os-software">SciexOS</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/markerview-software">MarkerView</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/lightsight-software">LightSight</a> and <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/molecule-profiler-software">MoleculeProfiler</a> software.</p><h5><strong>Training program</strong></h5><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part</strong></h5><ul><li>Principle of operation of MS/MS systems</li><li>Differences and capabilities of QTRAP and QTOF</li><li>Comparison of typical analytical procedures and their application</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><p><strong>Objective - to develop methods to search for metabolites of a known substance.</strong></p><h5><strong>QTRAP</strong></h5><ul><li>Development of IDA screening methods (pMRM, Prec/NL, EMS)</li><li>Optimization of LC-MS/MS IDA method</li><li>Data acquisition</li></ul><h5><strong>QTOF</strong></h5><ul><li>Development and optimization of LC-MS/MS IDA and SWATH methods</li><li>Data acquisition</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><p><strong>Objective - identification and profiling of metabolites of a known substance</strong></p><h5><strong>QTRAP data analysis</strong></h5><ul><li>Manual search and identification</li><li>LightSight, MarkerView</li></ul><h5><strong>QTOF data analysis</strong></h5><ul><li>Search and data analysis methods</li><li>Manual search and identification</li><li>IDA vs SWATH</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li></ul><p>Summary and discussion<br>&nbsp;</p>

Bioanalytic QTOF quantitative and qualitative analysis training

The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE:&nbsp;<strong> QTOF quantitative and qualitative analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX</strong><span class="ts-pf-details-content"><strong> </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg" alt="Bioanalytic: Bioanalytic QTOF quantitative and qualitative analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg 221w," sizes="100vw" width="1000" height="707"></p><h4><strong>TRAINING COURSE DESCRIPTION</strong></h4><p><strong>Qualitative and quantitative analysis of QTOF</strong> - the training is designed for laboratory personnel using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=30&amp;mainauthorItems=55"><strong>high-resolution (HR) LC-MS/MS</strong></a> systems for qualitative and quantitative analysis. The following topics are covered during the training:</p><ul><li><strong>QTOF spectrometer calibration</strong></li><li><strong>Targeted and untargeted methods</strong></li><li><strong>Quantitative analysis (TOF MS, MRMhr)</strong></li><li><strong>Qualitative and quantitative analysis - DDA (IDA) vs DIA (SWATH) methodology</strong></li><li><strong>Software and its use in high-resolution data analysis</strong></li><li><strong>Reporting of results</strong></li></ul><p>The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis. To point out the advantages of high resolution in identifying or confirming the presence of substances in a sample and also the strengths of modern QTOF systems in parallel quantitative analysis.</p><h3><strong>Training program</strong></h3><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part</strong></h5><ul><li>The principle of operation and capabilities of the QTOF system</li><li>Comparison of typical analytical procedures and their application</li><li>Targeted and quantitative analysis (TOF MS vs MRMhr)</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><p><strong>Objective</strong> - to develop methods for quantitative analysis, confirmation or search for metabolites of a known substance, general characterization of the sample.</p><h5><strong>Practical part:</strong></h5><ul><li>Creation and optimization of the MRMhr targeted method</li><li>Creation and optimization of a non-targeted method IDA</li><li>Creation and optimization of the non-targeted method SWATH</li><li>Data acquisition (overnight)</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><p><strong>Objective</strong> - quantitative analysis, confirmation of the presence or identification of metabolites of a known substance, general characterization of the sample (identification of compounds present in the sample), profiling.</p><h5><strong>Practical part:</strong></h5><ul><li>Quantitative analysis - TOF MS vs MRMhr</li><li>Methods for searching and analyzing data</li><li>IDA vs SWATH data</li><li>Manual search and identification</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li><li>Summary and discussion</li></ul>

Watrex application and R&D laboratory

Watrex application and R&D laboratory is providing a range of analyses.

<p><strong>Watrex application and R&amp;D laboratory is providing a range of analyses with special focus in the following fields:</strong></p><ul><li>Chemical analysis for construction industry</li><li>Environmental analysis</li><li>Sample preparation including online solid-phase extraction protocols</li><li>Development of methods for pharmaceutical analysis</li><li>Characterization of synthetic polymers by GPC</li><li>Column technology and methods for chiral separation</li><li>Parameters evaluation and testing in ion chromatography applications</li></ul><h5><strong>Our ready-to-go tests include:</strong></h5><ul><li>Analysis of carbohydrates</li><li>Characterization of polymers</li><li>Analysis of organic acids</li><li>Construction analysis</li><li>Analysis of ions, pH and water hardness</li></ul>

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