<p>The pioneering research of Prof. Michal Holčapek and his team at the University of Pardubice is now being tested on patients in a clinical setting.</p><p><strong>A clinical trial has begun in the Czech Republic to assess the effectiveness of an early detection test for pancreatic cancer. This trial will evaluate the sensitivity of a novel blood test designed to detect precancerous changes. If successful, this lipidomics-based test could revolutionise pancreatic cancer screening, offering a major advance in early diagnosis.</strong></p><p>The trial is a collaborative effort between Lipidica – a company jointly owned by the University of Pardubice and FONS JK Group – and specialists in gastroenterology and oncology. Lipidica will perform sample analyses for the clinical study. Key partners include the University Hospital Olomouc and the Masaryk Memorial Cancer Institute, with a total of 15 centres nationwide expected to participate.</p><blockquote><h4><strong>Career: Postdoctoral Positions in Lipidomics and Glycomics</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/job/1713">more details and contacts</a></li></ul><p><strong>You will be joining the dynamic Lipidomics Group at the University of Pardubice, working on high-impact research funded by prestigious grants:</strong></p><ol><li><strong>ERC Advanced grant “Oncolipidomics:</strong> Why is lipidomic dysregulation pattern in blood similar for various cancers? (ONCOLIPID)”</li><li><strong>OP JAK grant “Saving lives through research in early cancer detection and prevention:</strong> Molecular, genomic and societal factors (SALVAGE)”</li></ol><h5><strong>Two Postdoctoral Positions in Lipidomics and Glycomics</strong></h5><ul><li><strong>Start Date:</strong> January 2025 (or as soon as possible)</li><li><strong>Duration:</strong> 12 months, with a possibility of extension until the end of the project (August 2028)</li><li><strong>Location:</strong> Lipidomics Group of Prof. Michal Holčapek, University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Czech Republic</li><li><strong>Salary:</strong> Competitive, based on university guidelines, and commensurate with experience and skills.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/University_of_Pardubice_University_of_Pardubice_254470ec4e.jpg" alt="University of Pardubice: University of Pardubice" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_University_of_Pardubice_University_of_Pardubice_254470ec4e.jpg 234w," sizes="100vw" width="1200" height="803"></p></blockquote><p>This methodology, which allows for early diagnosis, was developed by Prof. Michal Holčapek, a prominent analytical chemist and world-renowned expert in lipids at the University of Pardubice’s Faculty of Chemical Technology. Holčapek, a recipient of the prestigious European ERC Advanced grant, and his team achieved this breakthrough by analysing lipids in standard blood samples and applying advanced multivariate statistical analysis. <i><strong>"The lipidomics test is non-invasive and could pave the way for a structured screening programme,”</strong></i> explains Prof. Holčapek. <i><strong>"It may enable earlier detection of pancreatic cancer, where treatment success rates are higher and patient survival outcomes improve."</strong></i></p><p>This innovative method has been published in Nature Communications, one of the world’s leading scientific journals, and is protected by two patents held by the University of Pardubice.</p><p>Experts from the Czech Gastroenterological Society (CGS) report that approximately 2 400 people are diagnosed with pancreatic cancer each year in the Czech Republic, and nearly 2 200 die from the disease within the same period. By 2030, experts project that pancreatic cancer will become the second leading cause of cancer deaths. <i>"Pancreatic cancer is among the most aggressive forms of cancer – it develops asymptomatically over more than a decade but remains undetected until it is too advanced for effective treatment,"</i> says Prof. Ondřej Urban, M.D., Ph.D., Head of the Second Internal Gastroenterology and Geriatric Clinic at University Hospital Olomouc. <i>"By the time symptoms appear, it is often too late for curative treatment, with surgery remaining the only potential cure, but only about 20% of patients qualify. The rest receive palliative care focused on extending life with as much quality as possible."</i> For those with advanced pancreatic cancer, the average survival rate is about 1.5 years. However, patients diagnosed at an early stage can live up to six times longer. <i>"We know that lipid profiles vary significantly in patients with pancreatic cancer,"</i> Prof. Urban adds.<i> "This study aims to determine if the lipidomics test can detect these early lipid changes in high-risk individuals who feel healthy but may already have underlying disease."</i></p><p>According to doc. MUDr. Jan Trna, Ph.D., Chief Physician of the Gastroenterology Department at the Masaryk Memorial Cancer Institute, the successful validation of the new lipidomics test would represent a major breakthrough in pancreatic cancer treatment.<i> "Currently, our options for identifying individuals at risk for pancreatic cancer are limited. Due to financial, capacity, and medical constraints, specialised preventive testing cannot be offered to everyone. However, blood sampling presents a different approach. For decades, we have sought methods to detect the disease early – which is absolutely critical for this particular cancer," </i>says MUDr. Trna. The Pancreatic Cancer Early Detection Programme now enables the identification of precancerous changes or early-stage cancer. Under this programme, gastroenterologists collaborate closely with clinical geneticists and radiologists who conduct specialised tests such as magnetic resonance imaging (MRI).</p><p>Currently, the screening programme is available only to individuals with a lifetime risk of pancreatic cancer exceeding 5%. <i>"In the newly established centres of highly specialised care for digestive endoscopy, including our department, at-risk individuals undergo regular pancreatic examinations using MRI and endoscopic ultrasonography,"</i> explains doc. MUDr. Ilja Tachecí, Ph.D., Chair of the CGS and Head of the Second Internal Gastroenterology Clinic at the Faculty of Medicine, Charles University in Hradec Králové. Individuals considered at risk include those with a hereditary form of chronic pancreatitis, those with two or more direct relatives diagnosed with pancreatic cancer, and carriers of specific genetic mutations – such as mutations in the BRCA1 and BRCA2 genes, which are also associated with breast and ovarian cancer.</p><p><i>"People at risk visit us for annual check-ups. As part of this study, we collect blood samples with their informed consent, which are then processed into plasma and sent to the Lipidica laboratory,"</i> explains Prof. Urban. The study thus analyses blood from both healthy high-risk individuals and patients with small, operable tumours. <i>"Current diagnostic methods are highly effective but also costly and uncomfortable for patients. A test based on a simple blood draw would be a tremendous benefit for clinical practice. Our LDPC lipidomics test relies on a patent-protected methodology developed at the University of Pardubice, integrating insights from analytical chemistry, biostatistics, and other scientific disciplines,"</i> adds Ing. Karolína Kašparová, Director of Lipidica, which is also an accredited medical laboratory.</p><p><strong>Lipidica, a.s., a joint venture between the University of Pardubice and FONS JK Group a.s., has been </strong><a target="_blank" rel="noopener noreferrer" href="https://www.upce.cz/en/neuron-awards-goes-to-lipidica-spin-off-of-the-university-of-pardubice"><strong>recognised with the Neuron Prize</strong></a><strong> for its exceptional bridging of science and business.</strong></p><p><strong>The study began this year and is expected to conclude in 2027, involving over 400 participants. </strong>If the test's specificity and sensitivity are confirmed, experts note it may still take time before the test becomes a standard part of clinical care and before it is determined whether health insurers will cover it or if individuals will need to pay out of pocket. The manufacturer has indicated, however, that the price should not exceed a few thousand Czech korunas.&nbsp;</p>

Clinical trial begins in Czech Republic to test groundbreaking pancreatic cancer detection method

<blockquote><p><i>Maciej Stopa<sup>1, 2</sup>; Rafał Szewczyk<sup>1, 2</sup>; Adrianna Radulska<sup>3</sup>, Anna Lenartowicz<sup>2</sup>; Julia Mironenka<sup>2</sup>; Adrian Soboń<sup>1, 2</sup>; Katarzyna Krupczyńska-Stopa<sup>1, 2</sup>; Tomasz Borkowski<sup>3</sup>; Ewelina Marciniak<sup>3</sup>, Leszek Kalinowski<sup>3</sup></i></p><p><i><sup>1</sup></i><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315"><i><strong>Bioanalytic</strong></i></a><i><strong> </strong>Sp. z o.o., Gdansk, Poland; <sup>2</sup>LabExperts Sp. z o.o., Gdansk, Poland; <sup>3</sup> Medical University of Gdansk, Gdansk, Poland</i></p></blockquote><h4><strong>Abstract</strong></h4><p>Cardiac arrhythmia affects approximately 12.6% of people over the age of 65. Ventricular arrhythmias are considered as responsible for 75% to 80% of sudden cardiac deaths. Therapy of the disease include ablation, use of cardioverter or defibrillator and pharmacological treatment where the key for successful therapy lays in maintaining the necessary concentration of drugs. The goal can be achieved by therapeutic drug monitoring (TDM). Developed assay named “CardioCarePack” is based on the use of capillary blood collected and dried at home by a patient with volumetric absorptive microsampling (VAMS), quantitative analysis of selected drugs and their metabolites and a telemedical system that integrates all data between a doctor, patient and laboratory supporting the therapy process. CardioCarePack is ready for commercialization.</p><h4><strong>Methods</strong></h4><p>Blood collected with a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?distributorItems=315&amp;search=MITRA"><strong>MITRA® microsampling device</strong></a> (20 µl) is prepared with full validated protocol that include sonication with 0.1% formic acid in water followed by acetonitrile extraction, evaporation under nitrogen stream and resuspension in LC mobile phase. Sample is subjected to LC-MS/MS analysis in positive ionization MRM mode with QTRAP 5500+ mass spectrometer (SCIEX) or similar one. Analysis covers simultaneous quantitation of 15 drugs: Sotalol, Metoprolol, Bisoprolol, Propafenone, Carvedilol, Nebivolol, Amiodarone, Digoxin, Perindopril, Ramipril, Eplerenone, Spironolactone, Zofenopril and 2 metabolites: 5-hydroxypropafenone and Desethylamiodarone. Results are placed in a server-based telemedical system where depending on accession rights history, doses, therapeutic index flagging, cardiograms, sample tracking and many other data are available for patient, doctor and laboratory staff, respectively.</p><h4><strong>Preliminary Data</strong></h4><p>The project involved more than 300 patients who had been monitored for 2 years during regular pharmacological therapy. Every half a year during a visit in medical facility venous blood for serum testing and capillary blood on the MITRA® sorbent were collected. Between the visits patients were collecting samples by VAMS themselves at home. All samples were analyzed by fully validated procedure where among the others linearity (r ≥ 0.995), accuracy (80 – 120%), reproducibility (%CV ≤ 15%) for both MITRA® and serum samples were calculated. The LC-MS/MS method developed for 17 compounds covers therapeutic range of the tested drugs: 0.25 – 25 ng/ml (Digoxin and Nebivolol) 2.5 – 250 ng/ml (Metoprolol, Bisoprolol, Propafenone, Carvedilol, Perindopril, Ramipril, Spironolactone, Zofenopril and 25 – 2500 ng/ml (Sotalol, Desethylamiodarone, Eplerenone, Amiodarone, 5-hydroxypropafenone). Results generated during LC-MS/MS analysis were analyzed with SCIEX OS 1.7 software.</p><p>On the basis of samples collected during 4 visits in medical facility a correlation between drugs concentration in venous blood (serum) and capillary blood (MITRA®) (S/M) was calculated. For some compounds S/M ratio was close to 1 (ex. Sotalol – 0.95, %CV – 5.12%, Nebivolol – 1.03, %CV – 3.17%) or moderately to more than 2-fold different (ex. Digoxin – 0.69, %CV – 1.46%, Ramipril – 1.18, %CV – 11.1, Perindopril – 1.86, %CV – 4.73%, Amiodarone – 2.17, %CV – 10.78). The correlation factors are statistically significant (p &lt; 0.05) and can be used for accurate concentration estimation in venous blood.</p><p>Developed telemedicine system integrate CardioCarePack service – a patient, doctor and LC-MS/MS laboratory. The system helps in Doctor’s supervision over the patient’s condition on the basis of collected within the system data ex.: autonomous verification of the therapeutic index with flagging, visualization of results, tracking samples on the patient-doctor line, schedule of visits, results history and many more.</p><h4><strong>Novel aspect</strong></h4><p>A modern telemedicine system for TDM of cardiological drugs based on LC-MS/MS analysis of samples collected at home with VAMS.</p>

CardioCarePack – personalized medicine system to improve live quality of patients suffering from cardiac arrhythmias

<p><strong>In the week of January 13, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Waters Corporation: Light scattering-based solutions for excipients and advanced formulations</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 05:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4545">Registration</a></div></li></ul></div><p>In this webinar, we will showcase case studies demonstrating how SEC-MALS and FFF-MALS are utilized to characterize, quantify, and deformulate advanced excipients and formulations.</p></div><h5><strong>2.&nbsp;Thermo Fisher Scientific: Well characterized biological products, challenging yet rewarding</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4578">Registration</a></div></li></ul></div><p>The presentation emphasizes the importance of developing innovative methods and technologies to support the ecosystem and enhance data workflows. It introduces the concept of a new facility, named "Concept".</p></div><h5><strong>3.&nbsp;Agilent Technologies: Getting the Most from Your Agilent HPLC Pump</strong></h5><div><div><ul><li><div>Tu, 14.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4519">Registration</a></div></li></ul></div><p>We will focus on your Agilent HPLC pump. We will look at some of its capabilities and how to choose the right column for it. We will discuss best practices to help maximize up-time.</p></div><h5><strong>4.&nbsp;Restek Corporation: Incorporating ultrashort-chain compounds into the comprehensive analysis of PFAS</strong></h5><div><div><ul><li><div>We, 15.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4569">Registration</a></div></li></ul></div><p>Join Dr. Shun-Hsin Liang, as he explores the simultaneous analysis of C1 to C14 PFCA and PFSA, along with other PFAS types, using two alternative chromatographic approaches.</p></div><h5><strong>5.&nbsp;SCIEX: Unlocking precision: nitrosamine and NDSRI analysis</strong></h5><div><div><ul><li><div>Th, 16.1.2025 10:30 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4682">Registration</a></div></li></ul></div><p>We invite you to join our webinar on nitrosamine and NDSRIs, specifically examining their analysis and implications in the pharmaceutical industry.</p></div><h5><strong>6.&nbsp;Thermo Fisher Scientific: The merger of automated LC fractionation &amp; in-depth characterization</strong></h5><div><div><ul><li><div>Th, 16.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4579">Registration</a></div></li></ul></div><p>Dr. Kristensen discusses how the shift from traditional data handling methods to cloud solutions has revolutionized their workflow, enabling access to vast amounts of data from anywhere.</p></div><h5><strong>7.&nbsp;Waters Corporation: Mastering the Quantitative Bioanalysis of Large Peptides and Proteins: A Case Study of GLP-1 Receptor Agonist Semaglutide in Plasma</strong></h5><div><div><ul><li><div>Th, 16.1.2025 15:00 CEST</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4384">Registration</a></div></li></ul></div><p>We present new data demonstrating a comprehensive and streamlined approach to optimized LC-MS/MS quantification of peptides, addressing the common challenges.</p></div><h5><strong>8.&nbsp;Waters Corporation:</strong> <strong>Method Validation</strong></h5><div><div><ul><li><div>Th, 16.1.2025 19:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4669">Registration</a></div></li></ul></div><p>We´ll discuss practical guidance around accuracy, precision, limit of quantitation and limit of detection and next we´ll walk you through an Empower CDS tool.</p></div>

Webinars LabRulezLCMS Week 3/2025

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Bioanalytic started its operations in 1998. The company was born from a passion for science and the need to provide innovative solutions in the laboratory equipment market, from a small business to a dynamically developing company with an established position.

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Bioanalytic Development of LC-MS/MS method

We take an individual and factual approach to developing a new method and performing analyses.

<h4><strong>An experienced partner in the LC-MS/MS analysis industry</strong></h4><p>If you are interested in performing quantitative, qualitative or mixed analysis using LC-MS/MS analytical methods but lack such capabilities due to lack of equipment, “know-how” or are looking for a team to perform analysis for a project - you have come to the right place. We work on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315"><strong>equipment</strong></a> with high sensitivity, speed and specificity. Our team is a group of specialists with comprehensive experience in the industry. The LabExperts <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/zespol/">team</a> has participated in many scientific projects, R&amp;D and commercial implementations. We take an individual and factual approach to developing a new method and performing analyses:</p><ul><li>In the first stage, we get to know your requirements - to align expectations with capabilities;</li><li>Next, we will form and approve the framework for cooperation, ensuring the observance of confidentiality by signing a non-disclosure agreement (NDA);</li><li>We will plan together and, for our part, meet the deadlines for the completion of the set tasks;</li><li>We will perform preliminary studies, after which we will decide together whether and to what extent we continue to develop the methods;</li><li>We will handle the development of the sample preparation procedure;</li><li>We will select the best parameters for the LC-MS/MS analytical method;</li><li>The analytical method along with the sample preparation procedure will be validated according to generally accepted guidelines and standards, or we will establish the required acceptance criteria and ranges for your needs;</li><li>We will perform LC-MS/MS analyses of a series of samples;</li><li>We will provide results in the form of easy-to-read reports at each required stage;</li><li>We will evaluate the feasibility and assist in the transfer of the method to your laboratory.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg" alt="Bioanalytic: Bioanalytic Development of LC-MS-MS method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Development_of_LC_MS_MS_method_2594c9fd54.jpg 234w," sizes="100vw" width="1536" height="1024"></p><p>Pricing and the framework for cooperation are always determined individually depending on the scope of the study. This is influenced by the amount of work and the complexity of the development of the sample preparation procedure and analytical method and routine analytics. As a rule, the final price consists of three separately priced elements: the cost of preliminary studies, method validation, and the cost of analysis of one sample within a series of analyses.</p><p>Don't beat your head against the wall - we will help you achieve your goal!</p><p>We look forward to working with you!</p>

Bioanalytic Individual LC-MS/MS training

We are ready to prepare customized LC-MS/MS training for the needs of participants.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Customized LC-MS/MS Training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong> or other</li><li>TRAINING TIME: <strong>1-5 days</strong> or more</li><li>NUMBER OF PARTICIPANTS: <strong>4-8 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP/QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg" alt="Bioanalytic: Bioanalytic Individual LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg 245w," sizes="100vw" width="1536" height="930"></p><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Customized LC-MS/MS training we are ready to prepare for the needs of participants, discussing:</p><ul><li>theoretical basics of chromatography, mass spectrometry,</li><li>practical operation of the LC-MS/MS system,</li><li>qualitative and quantitative analysis,</li><li>DDA &amp; DIA screening methods,</li><li>“omics” analyses,</li><li>validation of analytical methods,</li><li>solving basic methodological and technical problems,</li><li>software,</li><li>calibration,</li><li>“tips&amp;tricks”,</li><li>etc...</li></ul><p>We offer training for both novices starting the fascinating adventure of LC-MS/MS analytics, as well as for those who are LC-MS/MS users and are interested in expanding and consolidating their knowledge.</p><h4><strong>Framework training program</strong></h4><ul><li>individual LC-MS/MS training is developed according to the customer's demand</li><li>conducting theoretical and/or practical training at the customer's workplace</li><li>carrying out theoretical and/or practical training in a laboratory in Lodz</li></ul>

Bioanalytic Basic LC-MS/MS training

Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Basic LC-MS/MS training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP </strong><span class="ts-pf-details-content"><strong>&amp; </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg" alt="Bioanalytic: Bioanalytic Basic LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg 205w," sizes="100vw" width="1293" height="987"></p><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument. Basic LC-MS/MS training is primarily aimed at those who are new to LC-MS/MS equipment or who wish to become familiar with this analytical technique. During the training, the following will be presented:</p><ul><li>principles of developing and optimizing quantitative and qualitative methods on low (triple quadrupole, QTRAP) and high resolution (QTOF) instruments,</li><li>basic functionality of the software,</li><li>procedures and objectives of instrument calibration,</li><li>principles of maintaining the cleanliness of mass spectrometer operation.</li></ul><h5><strong>Basic LC-MS/MS training - the program</strong></h5><p><span style="font-size:20px;"><strong>Day I</strong></span></p><p><strong>Theoretical classes:</strong></p><ul><li>Introduction to LC</li><li>Introduction to mass spectrometry (basics)</li><li>Quantitative analysis.</li></ul><p><strong>Practical part:</strong></p><p>QTRAP:</p><ul><li>QTRAP scanning modes on the instrument - Q1, , MS2, MRM, demonstration and discussion on the example of a selected pesticide of the fragmentation spectrum and its fragmentation pathway.</li><li>MRM pair formation (manual, semi-automatic, automatic).</li><li>optimization of ion source parameters -T-conector</li><li>explanation of the main parameters related to LC (column selection, gradient vs isocrat, autosampler)</li><li>LC-MS/MS method setup and optimization</li></ul><p>QTOF:</p><ul><li>manual and automatic optimization of MRM<sup>HR</sup></li><li>ion source and LC parameters (transfer from method developed above)</li></ul><p>Sequence of quantitative analysis on both instruments.</p><p><span style="font-size:20px;"><strong>Day II</strong></span></p><p><strong>Quantitative analysis:</strong></p><ul><li>data processing and analysis,</li><li>reporting - Sciex OS (QTRAP, QTOF).</li><li>Discussion of types of integration algorithms (MQ4, autopeak) and integration parameters.</li><li>independent selection of integration parameters and plotting of master curves.</li><li>independent data analysis and generation of analysis report.</li></ul><p><strong>Qualitative analysis:</strong></p><ul><li>Theoretical introduction - introduction to qualitative analysis:</li><li>qwadrupole modes (Prec/NL),</li><li>trap modes (EMS, EPI, MS3),</li><li>QTOF modes (TOF MS, TOF MS/MS).</li><li>mixed methods,</li><li>targeted vs. untargeted analysis,</li><li>DDA (IDA) - discussion of method development,</li></ul><p><strong>Practical part:</strong></p><ul><li>creation of qualitative methods on QTRAP (Prec-IDA, MRM-IDA), other parameters (LC, ion source) as the previous day,</li><li>LightSight - discussion of operation, creation of MRM-IDA multimethod for selected pesticide and analysis of samples (night).</li><li>QTOF - creation of IDA method, other parameters (LC, ion source) as the previous day.</li></ul><p>Sequence of qualitative analysis overnight.</p><p><span style="font-size:20px;"><strong>Day III</strong></span></p><p><strong>Qualitative data analysis:</strong></p><p>QTRAP:</p><ul><li>Discussion of Prec-IDA, MRM-IDA results - what we see in different types of MS and MS/MS scans. Manual review of data and via IDA Explorer (SciexOS).</li><li>Analysis of results in LightSight, comparison with Prec-IDA, for example - advantages/disadvantages</li></ul><p>QTOF:</p><ul><li>search for potentially present compounds in the sample,</li><li>manual comparison of blank vs. test sample,</li><li>manual by XIC extraction (by sum formula),</li><li>Formula finder - derivation of sum formula on TOF MS data and verification of fragmentation on TOF MS/MS data</li><li>search in IDA Explorer mode - application of filters: similarity, isotopic distribution, mass defect,</li><li>database searching,</li><li>Molecule Profiler - demonstration of software capabilities on selected pesticide example,</li></ul><p><strong>Instrument calibration:</strong></p><ul><li>resolution, mass-to-charge ratio, intensity,</li><li>Q1 and Q3, LIT (MS and MS/MS), TOF MS and TOF-MS/MS</li><li>Automation vs. manual calibration,</li></ul><p><strong>LC-MS/MS application examples</strong></p><p><strong>Summary</strong></p><p><strong>Discussion</strong></p>

Bioanalytic Quantitative LC-MS/MS analysis training

The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>Quantitative LC-MS/MS analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP LC-MS/MS</strong></li></ul><figure class="image"><img style="aspect-ratio:1200/675;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg" alt="Bioanalytic: Bioanalytic Quantitative LC-MS-MS analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg 245w," sizes="100vw" width="1200" height="675"></figure><h4><strong>TRAINING DESCRIPTION</strong></h4><p>Quantitative LC-MS/MS analysis - the training is designed for laboratory personnel using LC-MS/MS systems for quantitative analysis. The following topics are covered during the training:</p><ul><li>Triple quadrupole spectrometer calibration</li><li>MRM pair formation and optimization</li><li>Optimization of ion source parameters</li><li>Optimization of chromatographic parameters</li><li>Sample preparation using the example of blood drawn on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1283"><strong>Mitra®</strong></a> samplers (VAMS)</li><li>Matrix effect, internal standards (ISTD), matrix curves</li><li>Validation of the quantitative method</li><li>Reporting of results</li></ul><p>The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis while covering the widest possible linear range of concentrations.</p><h3><strong>Training program</strong></h3><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part:</strong></h5><p>Triple quadrupole structure, ionization, MRM and sMRM scanning, chromatography, multimethods</p><h5><strong>Practical part:</strong></h5><ul><li>Q1/Q3 calibration (10 Da/s), MS/MS - resolution, m/z, intensity.</li><li>MRM pair formation and optimization (manual, semi-automatic, automatic).</li><li>Ion source optimization - T-concector and FIA</li><li>Gradient vs isocrat, dosing, column selection, temperature selection, other important parameters</li><li>Establishment and optimization of LC-MS/MS method.</li><li>How to take care of MS cleanliness - ionization time programming, multi-way valve, cleaning of ion source and instrument front end</li></ul><p>Sample preparation in pure solvent (standard curve, QC, solvent, solvent+ISTD, test samples), overnight analysis sequence.</p><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><h5><strong>Practical part - preparation of whole blood samples (Mitra®):</strong></h5><ul><li>Preparation of matrix standard curves</li><li>Preparation of quality control (QC) samples in three concentrations</li><li>Preparation of blank samples (without ISTD and with ISTD).</li><li>Preparation of samples for recovery determination (“pre-spiked” and “post-spiked”)</li><li>Preparation of test samples</li></ul><h5><strong>Theoretical part:</strong></h5><ul><li>Samples in the matrix - discussion of the matrix effect and ways to level it.</li><li>Optimization of sample preparation method and LC-MS/MS method</li></ul><h5><strong>Practical part:</strong></h5><ul><li>Sample extraction from Mitra® samplers for LC-MS/MS analysis.</li><li>Sequence of analysis of prepared samples overnight</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><h5><strong>Theoretical part:</strong></h5><ul><li>Validation of the quantitative LC-MS/MS method</li></ul><h5><strong>Practical part:</strong></h5><ul><li>Software - data integration algorithms, signal smoothing, weighing.</li><li>Matrix curves vs curves in solvent, influence of ISTD (Sciex OS).</li><li>Method validation - validation criteria, determination of LOD, LLOQ, ULOQ, linearity, working range of the method, repeatability, recovery.</li><li>Preparation of summary validation reports</li><li>Reporting of results from test samples - report vs export of data to Excel.</li><li>Summary and discussion.</li></ul>

Bioanalytic QTRAP vs QTOF Quality analysis training

The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE: <strong>QTRAP vs QTOF Quality analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX QTRAP </strong><span class="ts-pf-details-content"><strong>&amp; </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg" alt="Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg 221w," sizes="100vw" width="1000" height="707"></p><h4><strong>TRAINING COURSE DESCRIPTION</strong></h4><p><strong>QTRAP vs QTOF qualitative analysis</strong> - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of <strong>DDA (IDA)</strong> (QTRAP &amp; QTOF) and <strong>DIA (SWATH) </strong>(QTOF) methods. Analysis and comparison of results will be conducted using <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/sciex-os-software">SciexOS</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/markerview-software">MarkerView</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/lightsight-software">LightSight</a> and <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/molecule-profiler-software">MoleculeProfiler</a> software.</p><h5><strong>Training program</strong></h5><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part</strong></h5><ul><li>Principle of operation of MS/MS systems</li><li>Differences and capabilities of QTRAP and QTOF</li><li>Comparison of typical analytical procedures and their application</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><p><strong>Objective - to develop methods to search for metabolites of a known substance.</strong></p><h5><strong>QTRAP</strong></h5><ul><li>Development of IDA screening methods (pMRM, Prec/NL, EMS)</li><li>Optimization of LC-MS/MS IDA method</li><li>Data acquisition</li></ul><h5><strong>QTOF</strong></h5><ul><li>Development and optimization of LC-MS/MS IDA and SWATH methods</li><li>Data acquisition</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><p><strong>Objective - identification and profiling of metabolites of a known substance</strong></p><h5><strong>QTRAP data analysis</strong></h5><ul><li>Manual search and identification</li><li>LightSight, MarkerView</li></ul><h5><strong>QTOF data analysis</strong></h5><ul><li>Search and data analysis methods</li><li>Manual search and identification</li><li>IDA vs SWATH</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li></ul><p>Summary and discussion<br>&nbsp;</p>

Bioanalytic QTOF quantitative and qualitative analysis training

The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis.

<h4><strong>BASIC INFORMATION</strong></h4><ul><li>TITLE:&nbsp;<strong> QTOF quantitative and qualitative analysis training</strong></li><li>CATEGORY: <strong>LC-MS/MS training</strong></li><li>LOCATION: <strong>Lodz, PL</strong></li><li>TRAINING TIME: <strong>3 days</strong></li><li>NUMBER OF PARTICIPANTS: <strong>up to 5 people</strong></li><li>EQUIPMENT:<strong> SCIEX</strong><span class="ts-pf-details-content"><strong> </strong></span><strong>QTOF LC-MS/MS</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg" alt="Bioanalytic: Bioanalytic QTOF quantitative and qualitative analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg 221w," sizes="100vw" width="1000" height="707"></p><h4><strong>TRAINING COURSE DESCRIPTION</strong></h4><p><strong>Qualitative and quantitative analysis of QTOF</strong> - the training is designed for laboratory personnel using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=30&amp;mainauthorItems=55"><strong>high-resolution (HR) LC-MS/MS</strong></a> systems for qualitative and quantitative analysis. The following topics are covered during the training:</p><ul><li><strong>QTOF spectrometer calibration</strong></li><li><strong>Targeted and untargeted methods</strong></li><li><strong>Quantitative analysis (TOF MS, MRMhr)</strong></li><li><strong>Qualitative and quantitative analysis - DDA (IDA) vs DIA (SWATH) methodology</strong></li><li><strong>Software and its use in high-resolution data analysis</strong></li><li><strong>Reporting of results</strong></li></ul><p>The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis. To point out the advantages of high resolution in identifying or confirming the presence of substances in a sample and also the strengths of modern QTOF systems in parallel quantitative analysis.</p><h3><strong>Training program</strong></h3><h4><span style="font-size:20px;"><strong>Day I</strong></span></h4><h5><strong>Theoretical part</strong></h5><ul><li>The principle of operation and capabilities of the QTOF system</li><li>Comparison of typical analytical procedures and their application</li><li>Targeted and quantitative analysis (TOF MS vs MRMhr)</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4><span style="font-size:20px;"><strong>Day II</strong></span></h4><p><strong>Objective</strong> - to develop methods for quantitative analysis, confirmation or search for metabolites of a known substance, general characterization of the sample.</p><h5><strong>Practical part:</strong></h5><ul><li>Creation and optimization of the MRMhr targeted method</li><li>Creation and optimization of a non-targeted method IDA</li><li>Creation and optimization of the non-targeted method SWATH</li><li>Data acquisition (overnight)</li></ul><h4><span style="font-size:20px;"><strong>Day III</strong></span></h4><p><strong>Objective</strong> - quantitative analysis, confirmation of the presence or identification of metabolites of a known substance, general characterization of the sample (identification of compounds present in the sample), profiling.</p><h5><strong>Practical part:</strong></h5><ul><li>Quantitative analysis - TOF MS vs MRMhr</li><li>Methods for searching and analyzing data</li><li>IDA vs SWATH data</li><li>Manual search and identification</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li><li>Summary and discussion</li></ul>

Watrex application and R&D laboratory

Watrex application and R&D laboratory is providing a range of analyses.

<p><strong>Watrex application and R&amp;D laboratory is providing a range of analyses with special focus in the following fields:</strong></p><ul><li>Chemical analysis for construction industry</li><li>Environmental analysis</li><li>Sample preparation including online solid-phase extraction protocols</li><li>Development of methods for pharmaceutical analysis</li><li>Characterization of synthetic polymers by GPC</li><li>Column technology and methods for chiral separation</li><li>Parameters evaluation and testing in ion chromatography applications</li></ul><h5><strong>Our ready-to-go tests include:</strong></h5><ul><li>Analysis of carbohydrates</li><li>Characterization of polymers</li><li>Analysis of organic acids</li><li>Construction analysis</li><li>Analysis of ions, pH and water hardness</li></ul>

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