Derivatizing amino acids doesn’t have to take hours to do. A more hands-off solution can save your lab hours of prep work. And help you trust your data even more.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_f41f58db04.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_f41f58db04.jpeg 245w," sizes="100vw" width="1024" height="512">Ever wonder what makes a simple molecule like valine so complicated to measure?Amino acids—the building blocks of proteins—are everywhere. In your food. Body. And even the fertilizers that help grow our food. But measuring these compounds precisely? That’s a classic analytical headache, even for an experienced chromatographer.Ask any lab scientist who’s done amino acid analysis, and they’ll tell you the chromatographic separation isn’t the hard part. The challenge is sample preparation, or everything before the chromatography. Handling toxic reagents. The repeated pipetting. And then timing the derivatization reactions just right.All these manual steps can contribute to lower method precision and data reproducibility.So, what’s the solution? Using a more hands-off approach. You get the same trusted chemical reaction, but your method is now faster, cleaner, and more reliable.Let’s walk through a real-life example of how your lab can derivatize amino acids directly inside the HPLC autosampler needle.<h3>Key takeaways</h3><div><ul><li>Manual derivatization of amino acids is laborious, error-prone, and handling toxic chemicals can pose health hazards to lab staff.</li><li>Automated in-needle OPA/FMOC derivatization of amino acids is a time- and labor-saving approach for HPLC-FLD analysis.</li><li>Reliable method performance, column stability over several hundred injections, and using inexpensive reagents facilitates high sample throughput at low costs per sample for routine laboratories.</li><li>Sample matrices of medium and high complexity (beverages, food, fertilizers) using in-needle derivatization is robust and reproducible.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_2_a906250f79.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids: Illustration showing manual derivatization versus in-needle derivatization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_2_a906250f79.jpeg 245w," sizes="100vw" width="700" height="410"><h3>Why does the derivatisation of amino acids still matter when aerosol detectors are an option? The unmatched sensitivity of fluorescence detection compared to evaporative technology.</h3>Most amino acids don’t have double bonds that naturally absorb UV light, meaning your HPLC detector can’t “see” the compounds directly.To solve these visibility issues, chemists use derivatization. The conjugation reaction is brilliant chemistry, facilitating the formation of <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work.html">fluorescent derivatives</a> suitable for sensitive and precise analysis.This technique involves reacting amino acids in the solution for analysis with reagents that make the amino acids fluorescent. The most common pair are OPA (ortho-phthaldialdehyde) for primary amino acids and FMOC-Cl (9-fluorenylmethyloxycarbonyl chloride) for secondary ones.Now here’s what can go wrong. You measure reagents, mix samples by hand, wait for the reaction, then inject onto your HPLC instrument. Small inconsistencies, such as a few seconds too long or a reagent variation, can mean BIG differences in peak response, method accuracy, and data reproducibility. Removing these inconsistencies is where automated in-needle derivatization comes in.<h3>Let your HPLC autosampler needle handle the not-so-friendly laborious tasks</h3>Switching from manual to automated in-needle derivatization can help make this labor-intensive process easier for everyone in your lab, while improving data consistency and overall lab safety. &nbsp;Here’s how:<div><div><ul><li>Instead of manually preparing reaction mixtures, you can use a compatible instrument, like<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=37&amp;manufacturerItems=3&amp;search=vanquish"> Thermo Scientific Vanquish LC Systems</a>, to perform in-needle derivatization.</li><li>And, with the helpful guidance of supporting software, you can automatically mix samples and reagents in the needle, time the reaction more precisely, and inject the derivatized sample onto the column.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_3_5c636b1584.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids: In-needle derivatization of primary and secondary AAs with OPA/MPA and FMOC-Cl reagents for subsequent LC analysis of the fluorescent derivatives. *Borate buffer pH 10, **acetic acid for quenching." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_3_5c636b1584.jpeg 245w," sizes="100vw" width="768" height="384"><h3>A real-life example showcasing better efficiency, robustness, and throughput capabilities.</h3>What makes this automated workflow stand out isn’t a new reaction—but rather a faster way of working with your HPLC instrument.The reactions between OPA/MPA and FMOC-Cl still happen as before, but now the reaction is controlled digitally through <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_003698_hplc_vanquish_workflow_amino_acid_analysis_derivatization_cn003698_na_en_e76e3abe44.pdf">Thermo Scientific Chromeleon Chromatography Data System</a> (CDS).As demonstrated in our whitepaper, each reagent draw, mix, and injection step is defined in a Custom Injection Program (CIP), eliminating timing errors and variability. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a> visualizes every step in its Instrument Method Editor, tracks parameters in real time, and logs events in the Audit Trail for compliance.And since the workflow design has flexibility in mind, you can use Chromeleon CDS or a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ab_004093_hplc_vanquish_core_amino_acids_needle_derivatization_ab004093_na_en_edb37df857.pdf">third-party software</a>, with identical parameters, which gives your labs the freedom to work within your preferred environment without retraining staff or rewriting SOPs.<h3>Trusting your data and a faster derivatization technique. That’s the science behind putting your sample preparation inside the needle.</h3>The most beneficial part of this workflow is the hours of time your lab and broader QC team will save.<ul><li>Consistent fluorescence response and accurate quantitation for all amino acids</li><li>Excellent injection-to-injection reproducibility, even across reagent batches</li><li>Improved safety through reduced manual solvent handling</li><li>Less hands-on time, freeing analysts for higher-value work</li><li>Seamless CDS integration for full audit traceability</li></ul>So next time you’re pipetting derivatization reagents by hand, secretly wishing there was a better way, why not consider an automated method approach?You get the same chemistry, performed automatically inside the autosampler, hands-free, ready to inject in minutes with reproducible data that you can trust.Learn more about this new automated amino acid analytical workflow in our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_003698_hplc_vanquish_workflow_amino_acid_analysis_derivatization_cn003698_na_en_e76e3abe44.pdf">customer application note today</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_4_341aed0b3a.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids: Chromatographic results of calibration standard (50 µM) measured with the Vanquish Core HPLC system, comparing method performance on 1st and 14th day of measurements." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_4_341aed0b3a.jpeg 245w," sizes="100vw" width="768" height="384"><h3>Helpful tips to improve method robustness with Vanquish LC Systems+ Chromeleon CDS</h3><ol><li>Use a 100 µL sample loop for thorough and reproducible mixing of the sample and reagents during the derivatization step.</li><li>Install a passive pre-heater to facilitate proper dilution of the reagents before the column.</li><li>Use guard cartridges for your columns and replace the cartridges when column performance diminishes. Apply smart tools like intelligent run control in Chromeleon CDS for automated checks.</li><li>Replace derivatization reagents in your autosampler daily.</li><li>Periodically flush your column with 100% acetonitrile for 30 minutes to remove persistent reaction products and matrices.</li></ol><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#AutomatedSamplePreparation #HPLC</blockquote></div></div></div>

How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids

<h3>How long does it actually take to install all the capillaries and fittings on a conventional HPLC system?</h3><h4>The Small Components That Matter Most</h4>When people think about HPLC systems, they often focus on pumps, detectors, columns, or software. Capillaries and fittings are usually seen as minor components because they are relatively inexpensive and physically small. However, every chromatographer eventually discovers that these tiny parts can have a major influence on system performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Connecting_a_U_HPLC_system_can_be_difficult_at_some_poorly_accessible_locations_3b837b441b.webp" alt="KNAUER: Connecting a (U)HPLC system can be difficult at some poorly accessible locations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Connecting_a_U_HPLC_system_can_be_difficult_at_some_poorly_accessible_locations_3b837b441b.webp 208w," sizes="100vw" width="720" height="540">Even decades ago, chromatographers recognized the importance of proper connections. In the 1980s, long before UHPLC became common, John Dolan wrote about minimizing dead volume through correct fitting installation. Today, with UHPLC systems operating at extremely high pressures and using columns with very small particle sizes, dead volume is even less tolerable than before. The topic remains just as relevant today as it was back then.Interestingly, despite what many users assume, the chromatographic column itself is often not the primary source of HPLC or UHPLC problems. In my experience, poor connections are far more commonly responsible for issues such as peak broadening, leaks, or loss of efficiency.<h4>The Early Days of HPLC Capillaries</h4>Anyone who has been working with HPLC for many years will probably remember the era of rigid 1/16" stainless steel tubing. Whenever PEEK tubing was unsuitable, stainless steel capillaries were the standard choice.Setting up a system back then was far from convenient. You first had to locate an appropriate length of tubing somewhere in a drawer full of capillaries. Usually, this meant finding a several-meter-long piece and cutting it yourself using a dedicated capillary cutter. After cutting, you needed to ensure the tubing end was smooth and undamaged, hoping that the previous person using the cutter had not accidentally crushed the inner diameter.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Standard_1_16_Capillary_and_needed_tools_and_the_drawer_we_all_know_pretty_well_943d7ed0b2.jpg" alt="KNAUER: Standard 1/16” Capillary and needed tools and the drawer we all know pretty well" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Standard_1_16_Capillary_and_needed_tools_and_the_drawer_we_all_know_pretty_well_943d7ed0b2.jpg 245w," sizes="100vw" width="1277" height="456">Once cut, the tubing often required additional preparation. You might need to deburr the capillary using a grater, shape it carefully with bending tools, and then finally install it using separate fittings and ferrules. And of course, everyone remembers the frustration of dropping a ferrule onto the laboratory floor, where it effectively disappeared forever.At the same time, laboratories often accumulated drawers filled with pre-assembled capillaries that had fixed fittings attached. Reusing these assemblies was risky. If the fitting geometry did not perfectly match the receiving port, users could unintentionally introduce dead volume into the system. The result was often degraded chromatographic performance, broad peaks, and frustrating troubleshooting sessions involving the entire laboratory team. In worse cases, excessive force could damage threads or receiving ports while attempting to stop leaks.During that period, assembling and connecting a complete HPLC system could easily consume half a day of work.<h4>The Improvement: Flexible and Pre-Cut Capillaries</h4>Later, laboratories gradually adopted pre-cut tubing and flexible capillaries, which represented a significant improvement over manually preparing stainless steel tubing.With these newer capillaries, users simply selected the correct length and installed the tubing directly. Flexible capillaries adapted naturally to the required shape, eliminating much of the manual bending and shaping work. Installation became faster, easier, and far less stressful.Still, these systems had limitations. Although the capillary itself improved, the fitting technology remained largely unchanged. Many of these capillaries were still effectively single-use components. If a connection was installed incorrectly because the tubing was not fully inserted into the receiving port before tightening, the result was often another unusable capillary destined for the growing “capillary drawer.” Sustainability was certainly not a strong point of these systems.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_A_typical_capillary_drawer_It_was_tricky_to_open_and_close_it_sometimes_530dd2087c.webp" alt="KNAUER: A typical capillary drawer. It was tricky to open and close it sometimes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_A_typical_capillary_drawer_It_was_tricky_to_open_and_close_it_sometimes_530dd2087c.webp 208w," sizes="100vw" width="1024" height="768">Even with these advancements, system setup still required considerable time and effort.<h4>The Real Breakthrough: Modern Tool-Free Connection Systems</h4>A major breakthrough finally arrived when manufacturers introduced a completely redesigned fitting and capillary concept aimed specifically at simplifying UHPLC connections.These newer systems came with fittings pre-installed directly on the capillary. Unlike traditional ferrule-based systems, the fittings automatically adjusted to different port depths and sealed directly at the bottom of the port. This dramatically reduced the amount of torque required and eliminated the need for complicated installation techniques.Perhaps the biggest advantage was convenience. These capillaries could be connected and disconnected more than 100 times without tools, while remaining entirely finger-tight. And best of all, ferrules no longer disappeared onto the laboratory floor.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_The_little_things_that_do_the_click_3a0469d516.jpg" alt="KNAUER: The little things that do the “click”" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_The_little_things_that_do_the_click_3a0469d516.jpg 245w," sizes="100vw" width="1866" height="1022">The system I currently use includes an especially useful feature: a patented torque-limiting mechanism that prevents both under-tightening and over-tightening. Once the correct torque is reached, the fitting produces a tactile “click,” providing immediate feedback that the connection is secure and properly installed every time.<h4>From Half a Day to Fifteen Minutes</h4>Thanks to these modern connection systems and pre-configured startup kits, installing an HPLC or UHPLC system today is dramatically faster than it used to be.What once consumed hours of work can now be completed in less than 15 minutes. The improvements in usability, reproducibility, and sustainability over recent years have been remarkable.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_e62b80154b.webp" alt="KNAUER: Trouble-free connection systom for HPLC and UHPLC" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_e62b80154b.webp 221w," sizes="100vw" width="690" height="487"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_2_71c24acdf5.webp" alt="KNAUER: Trouble-free connection systom for HPLC and UHPLC" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_2_71c24acdf5.webp 224w," sizes="100vw" width="1920" height="1339">And while today’s solutions already represent a huge leap forward, the evolution of HPLC connection technology is certainly not over yet. Looking ahead, it will be exciting to see how future innovations continue to simplify chromatography workflows even further.<blockquote>If you want to learn more about KNAUER´s capillaries, fittings and (U)HPLC system start-up kits and are interested in details and numbers, I highly recommend our brand <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/r75ev6rjivdbid246k60zrs3gjpvwdi5b8em_fad0f92fd9.pdf">new tech note "Benefits of KNAUER´s trouble-free analytical capillaries in terms of HPLC and UHPLC system performance</a>" and a visit to our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2402">products online</a>.</blockquote>

Let´s Connect a whole (U)HPLC system without tools and trouble

News from LabRulezLCMS Library - Week 21, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Phenomenex Kinetex Core-Shell C8 HPLC Columns

The Kinetex C8 HPLC column is a core-shell-based particle with a bonded silica phase ideal for the analysis of very hydrophobic compounds. 

Hydrophilic Polar Selectivity and Performance!The Kinetex C8 HPLC column is a core-shell-based particle with a bonded silica phase ideal for the analysis of very hydrophobic compounds. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run-time, increase sensitivity, and even provide greater resolution with much higher efﬁciency levels.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems*</li><li>Balancing Performance &amp; Retention</li><li>USP:L7</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp" alt="Phenomenex: Kinetex Core-Shell C8 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 8 (effective), 8 (effective) %</li><li>Surface Area (m²/g): 200</li><li>Particle Size (μm): 1.7, 2.6, 5</li><li>Recommended Use: Minimized hydrophobic retention for highly hydrophobic compounds</li></ul><h4>Balancing Speed and Retention</h4>The Kinetex core-shell C8 provides the same Kinetex performance but with a little less hydrophobic retention in comparison to the Kinetex C18 column. This C8 column is ideal for extremely hydrophobic compounds that might be too strongly retained on a traditional C18 phase column.<blockquote>Stationary phase Kinetex Core-Shell C8 is provided in these column types: analytical and preparative</blockquote><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Luna C8(2) HPLC Columns

The C8 column provides less hydrophobic retention than our C18 columns, but the density of the ligand bonding creates more steric based selectivity.

The C8 column provides less hydrophobic retention than our C18 columns, but the density of the ligand bonding creates more steric based selectivity.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>C8(2) Ultra-pure, metal-free silica (99.99% purity) (USP:L7)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp" alt="Phenomenex: Luna C8(2) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 13.5, 13.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the back bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Common Applications:</h4><ul><li>Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine</li></ul><blockquote>Stationary phase Luna C8(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna C8(2) HPLC Microbore, Minibore, MidBore, Analytical and Semi-preparative Columns are available with these parameters:</h5>Microbore Columns<ul><li>Column length (mm): 50, 150</li><li>Column ID (mm): 1</li><li>Particle size (µm): 3, 5</li></ul>Minibore Columns<ul><li>Column length (mm): 30, 50, 100, 150, 250</li><li>Column ID (mm): 2</li><li>Particle size (µm): 3, 5</li></ul>MidBore Columns<ul><li>Column length (mm): 30, 50, 150, 250</li><li>Column ID (mm): 3</li><li>Particle size (µm): 3, 5</li></ul>Analytical Columns<ul><li>Column length (mm): 30, 50, 75, 100, 150, 250</li><li>Column ID (mm): 4.6</li><li>Particle size (µm): 3, 5</li></ul>Semi-Prep Columns<ul><li>Column length (mm): 250</li><li>Column ID (mm): 10</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Intact XB-C8 LC columns

Biozen Intact XB-C8 column contains skillfully manufactured large pore core shell particles that provide narrower, taller peaks in conjunction with higher resolution.

Impurity profiling and characterization of intact biologic fragments is a challenging undertaking because of the need to identify very small differences between variants. Both bioZen Intact columns contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target HC/LC, Fc/Fab, or isoforms.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_Intact_XB_C8_HPLC_Column_c2159e0847.webp" alt="Phenomenex: Biozen Intact XB-C8 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_Intact_XB_C8_HPLC_Column_c2159e0847.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Separation Mode: Reversed Phase</li><li>Particle size: 3.6</li><li>Recommended Use: Fast separation of intact and subunit separation with hydrophobic selectivity</li></ul><h3>Phenomenex Biozen Biologics LC Columns</h3>With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:<ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3>Biocompatible Flow Path with BioTi™ Hardware</h3>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"><h3>4 Advanced Particle Platforms</h3>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868">

Phenomenex Luna C8(2) HPLC Preparative Columns

The C8 column provides less hydrophobic retention than our C18 columns, but the density of the ligand bonding creates more steric based selectivity.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>C8(2) Ultra-pure, metal-free silica (99.99% purity) (USP:L7)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp" alt="Phenomenex: Luna C8(2) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 13.5, 13.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the back bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Common Applications:</h4><ul><li>Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine</li></ul><blockquote>Stationary phase Luna C8(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna C8(2) HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5, 10</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Kinetex Core-Shell C8 Preparative HPLC Columns

Hydrophilic Polar Selectivity and Performance!The Kinetex C8 HPLC column is a core-shell-based particle with a bonded silica phase ideal for the analysis of very hydrophobic compounds. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run-time, increase sensitivity, and even provide greater resolution with much higher efﬁciency levels.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems*</li><li>Balancing Performance &amp; Retention</li><li>USP:L7</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp" alt="Phenomenex: Kinetex Core-Shell C8 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 8 (effective), 8 (effective) %</li><li>Surface Area (m²/g): 200</li><li>Particle Size (μm): 5</li><li>Recommended Use: Minimized hydrophobic retention for highly hydrophobic compounds</li></ul><h4>Balancing Speed and Retention</h4>The Kinetex core-shell C8 provides the same Kinetex performance but with a little less hydrophobic retention in comparison to the Kinetex C18 column. This C8 column is ideal for extremely hydrophobic compounds that might be too strongly retained on a traditional C18 phase column.<blockquote>Stationary phase Kinetex Core-Shell C8 is provided in these column types: analytical and preparative</blockquote>Axia Packed Preparative Columns<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Gemini C8(3) HPLC Columns

Gemini C8(3) purification media is optimized for human insulin and its analogues, offering exceptional mechanical strength, high packing efficiency, and enhanced resistance to caustic regeneration conditions.

<h4>Optimized Purification Media for Human Insulin and It's Analogues</h4><ul><li>Enhanced Robustness Against Caustic Regeneration Conditions</li><li>High Packing Efficiencies</li><li>Incredible Mechanical Strength</li></ul>Typically insulin purification methodologies include vital steps for column efficiency tests, equilibration, loading, and multiple caustic washes. In particular, the high pH caustic washes are very important for the removal aggregate build up, however these wash steps typical diminish column lifetime quite substantially. With the improved chemical robustness and performance of Gemini C8(3) customers can now switch their focus to higher yields and purities and minimize operational down-time due to less frequent need to replace contaminated media.<h4>QC Tested to ensure consistent selectivity for Insulin and Impurity</h4>In conjunction with a media designed specifically for insulin purification, the Quality team at Phenomenex strives to ensure high reproducibility of Gemini 10µm C8(3) through numerous quality control steps throughout the manufacturing process, including a fit for purpose human insulin QC test. This elevated level of focus on quality ultimately results in a model product for large scale insulin purification.<h5>Key features:</h5><ul><li>Carbon Load: -</li><li>Surface Area (m²/g): -</li><li>Particle Size: 10</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Products from category C8 HPLC columns focused on Consumables from Phenomenex - page 1

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