<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

<p><strong>Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"></p><h4><strong>The Role of Chromatography in Forensic Food Analysis</strong></h4><p>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:</p><ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate<br>adulteration.</li></ul><p>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.</p><h4><strong>Isocratic vs. Gradient Elution</strong></h4><p>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:</p><ul><li><strong>Isocratic HPLC </strong>uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li><strong>Gradient HPLC</strong> changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul><p>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.</p><h4><strong>Watrex Praha and the Contribution of Modern Systems to Food Analysis</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha, s.r.o.</strong></a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.</p><p>Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline"><strong>Streamline™ product line</strong></a><strong> </strong>includes several HPLC system concepts suitable for food analysis:</p><ul><li><strong>Isocratic HPLC system</strong> – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li><strong>Binary gradient HPLC system</strong> – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures<br>(e.g., phenolic profiles of wine, components of aromatic extracts).</li><li><strong>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography)</strong> – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf"><strong>Product catalogue HERE</strong></a></p></blockquote><p>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.</p><h4><strong>Practical Examples of Adulteration Revealed by HPLC</strong></h4><ul><li><strong>Detection of olive oil dilution: </strong>HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li><strong>Estimation of sugar addition in honey:</strong> Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li><strong>Identification of aromatic adulterants in wine:</strong> Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4><strong>Conclusion</strong></h4><p>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.</p><blockquote><p>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true"><strong>LinkedIn</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague"><strong>Facebook</strong></a>) for more engaging topics.</p></blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

<p><strong>Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.</strong></p><h4><strong>In this episode, you'll learn:</strong></h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul><p>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.</p><h4><strong>Referenced Papers:</strong></h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul><p>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.</p><h3><strong>Video Transcription</strong></h3><h4><strong>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</strong></h4><h5><strong>Introduction</strong></h5><p>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.</p><h5><strong>Research Focus: Malaria Drug Discovery and Kinase Targets</strong></h5><p>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.</p><p>This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.</p><p>Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.</p><h5><strong>Chromatography in Protein Expression and Purification Workflows</strong></h5><p>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.</p><p>Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.</p><p>A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.</p><h5><strong>Integrating Size Exclusion Chromatography into the Workflow</strong></h5><p>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.</p><p>To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.</p><p>These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.</p><h5><strong>The Role of Mass Spectrometry in Biomolecular Characterization</strong></h5><p>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.</p><p>Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.</p><p>These approaches are essential for understanding how candidate drug targets function within complex biological systems.</p><h5><strong>Sample Preparation Challenges in Biological Research</strong></h5><p>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.</p><p>Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.</p><h5><strong>Advances in Chromatography and MS for Malaria Research</strong></h5><p>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.</p><p>In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.</p><p>Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.</p><h5><strong>Malaria, Drug Resistance, and Global Impact</strong></h5><p>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.</p><p>During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.</p><p>Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.</p><h5><strong>Advice for Early-Career Researchers</strong></h5><p>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.</p><p>Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.</p><p>Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.</p><h5><strong>Closing Remarks</strong></h5><p>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Waters XSelect HSS T3 HPLC Columns

Waters XSelect HSS T3 is a low-coverage C18 phase compatible with 100% aqueous mobile phase and should be first choice for polar and non-polar compounds. 

<p><strong>The XSelect HSS T3 is a low-coverage C18 phase compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The XSelect HSS T3 columns exhibit ultra-low MS bleed and are fully scalable and transferable to UPLC separations. </strong>Packed in ultra-low dispersion hardware, 2.5 µm <strong>eXtended Performance</strong> [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_HPLC_Column_e96c520ca3.jpg" alt="Waters: XSelect HSS T3 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_HPLC_Column_e96c520ca3.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>10000 psi (690 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Bonding Technology: </strong>T3</li><li><strong>Silanol Activity:</strong> Medium</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology: </strong>HSS</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 11 %</li></ul><h4><strong>The Waters XSelect HSS T3 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters XSelect HSS T3 Column with eConnect Column Tag</strong></h5><ul><li>The HSS T3 column is a fully endcapped, low-coverage C18 bonded-phase column designed to retain extremely polar compounds by reversed-phase in high aqueous mobile phases.</li><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: XSelect HSS T3 Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>XSelect Columns</strong></h3><h4><strong>Achieve unmatched reproducibility with a wide range of selectivity</strong></h4><p>Quickly and easily develop robust methods compatible with all modern chromatographic detection modes and transfer them from lab to lab with the <strong>XSelect Column family</strong>.</p><p>Designed to help you achieve reproducible and highly selective chromatographic separations, the XSelect Column family features <strong>two base particles technologies</strong>, <strong>Charged Surface Hybrid (CSH)</strong> and <strong>High Strength Silica (HSS)</strong>. With XSelect Columns, method development scientists like you can achieve a wide range of selectivities across all application areas, without compromising performance.</p><h4><strong>Overview</strong></h4><ul><li>Designed for selectivity and stability at low pH</li><li>Superior peak shape for basic compounds</li><li>Highest analyte mass loading available</li><li>Excellent batch-to-batch reproducibility</li><li>Ideal for rapid method development</li><li><strong>CSH Column chemistries: </strong>C18, Fluoro-Phenyl (PFP), and Phenyl-Hexyl, available in 2.5 XP, 3.5, 5 μm analytical and 10 μm OBD preparative sizes</li><li><strong>HSS Column chemistries:</strong> HSS C18, C18 SB, and T3, available in 2.5 XP, 3.5, and 5 μm particle sizes</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using XSelect stationary phases</li><li><strong>Recommended Use:</strong> For improving selectivity, peak shape, and retentivity in methods development.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Select_Columns_ff2c4ddb9e/Waters-XSelect-Columns.jpg" alt="Waters: XSelect Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Select_Columns_ff2c4ddb9e/thumbnail_Waters-XSelect-Columns.jpg 242w," sizes="100vw" width="900" height="580"></p>

Waters Atlantis dC18 HPLC Columns

Waters Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. 

<p><strong>Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Atlantis dC18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>3 pH</li><li><strong>pH Range Max: </strong>7 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Bonding Technology:</strong> dC18</li><li><strong>Silanol Activity:</strong> Medium</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 12 %</li></ul><h4><strong>The Waters Atlantis dC18 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Atlantis dC18 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Atlantis dC18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h5><strong>Waters Atlantis dC18 Intelligent Speed (IS) Column</strong></h5><ul><li>Experience exceptional liquid chromatography performance with the Atlantis dC18 Intelligent Speed (IS) Column—a cutting-edge, high-resolution solution designed for the efficient separation of a wide range of polar and non-polar compounds, catering to diverse applications such as peptide analysis, protein analysis, small molecule analysis, and drug discovery.</li><li>What sets the Atlantis dC18 Intelligent Speed (IS) Column apart is its <strong>innovative Intelligent Speed (IS) technology</strong>. This proprietary technology enables <strong>faster analysis times</strong> without compromising separation efficiency, making it an ideal choice for laboratories that require rapid analysis of large sample volumes and reliable lab equipment.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg" alt="Waters: Atlantis dC18 Intelligent Speed (IS) Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>Atlantis Columns</strong></h3><h4><strong>Reversed-phase and HILIC HPLC Columns for superior polar compound retention</strong></h4><p><strong>Polar analytes</strong> are difficult to retain using traditional <strong>reversed-phase (RP) chromatography</strong> as they often elute in the void volume of the column. You can solve this challenge today with <strong>Atlantis Columns</strong>, which <strong>retain</strong> and <strong>separate polar analytes</strong> under <strong>reversed phase </strong>and<strong> HILIC</strong> conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.</p><p>Available in <strong>T3</strong>, <strong>dC18</strong>, and the <strong>unbonded HILIC chemistries</strong>, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.&nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Retains and separates small, water-soluble polar organic compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents</li><li>Long column lifetimes using low-pH mobile phases</li><li>Fully scalable in 3, 5, and 10 μm particle sizes</li><li><strong>Recommended Use:</strong> For the reproducible retention and separation of a broad range of polar and non-polar compounds.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_HPLC_Columns_6651612161.jpg" alt="Waters: Atlantis HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_HPLC_Columns_6651612161.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters Atlantis T3 HPLC Columns

Waters Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules

<p><strong>Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules and should be your first choice when developing separations of polar and non-polar compounds.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Atlantis T3 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Bonding Technology:</strong> T3</li><li><strong>Silanol Activity:</strong> Medium</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 14 %</li></ul><h4><strong>The Waters Atlantis T3 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Atlantis T3 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Atlantis T3 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h5><strong>Waters Atlantis T3 Intelligent Speed (IS) Column</strong></h5><ul><li>Your first option when creating separations of polar and non-polar compounds should be Atlantis T3 columns, which are universal, silica-based, reversed-phase C18 columns that allow balanced retention of polar and hydrophobic molecules. A trifunctional C18 alkyl phase is used in the Atlantis T3 Column's innovative T3 bonding technique, which is bonded at a ligand density that encourages polar compound retention and compatibility with water mobile phases. When compared to the conventional trimethyl silane (TMS) end-capping, the patented T3 end-capping method is significantly more efficient. You get better polar compound retention and aqueous compatibility when the special end-capping and T3 bonding are combined.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg" alt="Waters: Atlantis T3 Intelligent Speed (IS) Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>Atlantis Columns</strong></h3><h4><strong>Reversed-phase and HILIC HPLC Columns for superior polar compound retention</strong></h4><p><strong>Polar analytes</strong> are difficult to retain using traditional <strong>reversed-phase (RP) chromatography</strong> as they often elute in the void volume of the column. You can solve this challenge today with <strong>Atlantis Columns</strong>, which <strong>retain</strong> and <strong>separate polar analytes</strong> under <strong>reversed phase </strong>and<strong> HILIC</strong> conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.</p><p>Available in <strong>T3</strong>, <strong>dC18</strong>, and the <strong>unbonded HILIC chemistries</strong>, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.&nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Retains and separates small, water-soluble polar organic compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents</li><li>Long column lifetimes using low-pH mobile phases</li><li>Fully scalable in 3, 5, and 10 μm particle sizes</li><li><strong>Recommended Use:</strong> For the reproducible retention and separation of a broad range of polar and non-polar compounds.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_HPLC_Columns_6651612161.jpg" alt="Waters: Atlantis HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_HPLC_Columns_6651612161.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters SunFire C18 HPLC Columns

Waters SunFire C18 columns are used for general purpose method development at low pH.

<p><strong>SunFire C18 columns are used for general purpose method development at low pH. The C18 ligand is highly retentive, especially for basic compounds and it is ideally suited for purification and impurity profile assays.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: SunFire C18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 16 %</li></ul><h4><strong>The Waters SunFire C18 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters SunFire C18 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: SunFire C18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h5><strong>Waters SunFire C18 Intelligent Speed (IS) Column</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg" alt="Waters: SunFire C18 Intelligent Speed (IS) Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>SunFire Columns</strong></h3><h4><strong>Superior mass loading at low pH</strong></h4><p><strong>High efficiency at a low pH</strong> can be challenging. Waters SunFire HPLC Columns, available in <strong>analytical</strong> and <strong>preparative dimensions</strong>, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.</p><p><strong>SunFire Analytical Columns</strong> are available in <strong>2.5 µm</strong> to <strong>10 µm </strong>particle sizes across two reversed-phase column chemistries, <strong>C18</strong> and <strong>C8</strong>, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in <strong>5</strong> and <strong>10 µm particle sizes</strong> for <strong>preparative normal-phase purification</strong>.</p><h4><strong>Overview</strong></h4><ul><li><strong>Exceptional loading capacity </strong>for both analytical and preparative columns</li><li><strong>Excellent low pH stability </strong>for longer lifetimes</li><li><strong>Superior peak shapes </strong>for faster and easier integration</li><li><strong>Low mass spectral (MS) bleed</strong>, for enhanced analyte MS signal</li><li><strong>Seamless scalability from analytical</strong> to optimum bed density (OBD) <strong>preparative columns</strong></li><li><strong>Recommended Use:</strong> For increased mass loading with excellent column stability.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sun_Fire_Columns_3f48bd770c/Waters-SunFire-Columns.jpg" alt="Waters: SunFire Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sun_Fire_Columns_3f48bd770c/thumbnail_Waters-SunFire-Columns.jpg 242w," sizes="100vw" width="900" height="580"></p>

Waters Symmetry C18 HPLC Columns

With Waters Symmetry C18 the monofunctionally bound C18 ligand included in these columns, you can increase efficiency and precision in your lab.

<p><strong>Primarily supports legacy methods. Symmetry columns give you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.</strong></p><p>With the <strong>Symmetry C18 Column</strong>, you will gain excellent <strong>reproducibility</strong> year after year. With each batch of new columns, there is no need to revalidate and redevelop techniques. The lab equipment enables the labor costs associated with standard analytical columns that require QC column testing to be reduced.</p><p>The <strong>Symmetry C18 Columns</strong> are manufactured in <strong>cGMP</strong>, <strong>ISO9001</strong>, and <strong>ISO13485</strong> certified facilities, ensuring that each step is monitored and the toughest criteria are maintained. They offer the tightest specifications in the market, ensuring consistent results every time. This is accomplished by designing Symmetry C18 Columns with high purity raw materials and column packaging techniques that provide the most repeatable HPLC column.</p><p>With the <strong>monofunctionally bound C18 ligand</strong> included in these columns, you can increase efficiency and precision in your lab. For optimum reproducibility, they additionally comprise a high purity base-deactivated silica. Symmetry C18 Columns also offer the industry's tightest ligand surface coverage (ligand density) standards, resulting in minimum resolution changes and more consistent batch-to-batch results.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Symmetry C18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 8.5 %</li></ul><h4><strong>The Waters Symmetry C18 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Symmetry C18 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Symmetry C18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h5><strong>Waters Symmetry Shield RP18 HPLC Column</strong></h5><ul><li>These reversed-phase columns contain Waters patented embedded polar group technology that “shields” the silica’ residual silanol surface from highly basic analytes. The reduced silanol activity for the SymmetryShield RP18 column significantly improves peak shape and resolution.</li><li><strong>Bonding Technology: </strong>Shield RP18</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>Carbon Load:</strong> 17 %</li></ul><h5><strong>Waters Symmetry Shield RP18 HPLC Column with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><h3><strong>Symmetry HPLC Columns</strong></h3><h4><strong>Silica-based columns that exceed HPLC column performance expectations</strong></h4><p><strong>Reproducible HPLC columns</strong> are essential when <strong>developing analytical methods</strong> for <strong>pharmaceutical </strong>and <strong>biopharmaceutical products</strong>. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.</p><p>Available in column and guard formats, <strong>Symmetry Columns</strong> are manufactured using <strong>high purity silica</strong> and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.</p><h4><strong>Overview</strong></h4><ul><li>Deliver maximum reproducibility batch-to-batch and column-to-column</li><li>Provide superior peak shape</li><li>Achieve high recoveries of peptides and proteins</li><li>Manufactured using high purity silica and tightly controlled manufacturing processes</li><li><strong>Recommended Use:</strong> For rugged and reproducible HPLC column performance.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_Columns_a2367d8095/Waters-Symmetry-Columns.jpg" alt="Waters: Symmetry Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_Columns_a2367d8095/thumbnail_Waters-Symmetry-Columns.jpg 223w," sizes="100vw" width="700" height="490"></p><h5><strong>Rugged and reproducible HPLC column performance</strong></h5><p>With Symmetry HPLC Columns, you’ll achieve reliable column-to-column and batch-to-batch reproducibility. Symmetry’s narrow column specification ranges minimize variation, giving you the confidence that the methods you use today will continue to be used in the future.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_HPLC_Colum_Unmatched_Year_to_year_Reproducibility_40b6d2de53.jpg" alt="Waters: Symmetry HPLC Columm - Unmatched Year to year Reproducibility" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_HPLC_Colum_Unmatched_Year_to_year_Reproducibility_40b6d2de53.jpg 146w," sizes="100vw" width="620" height="664"></p><h5><strong>Superior peak shape</strong></h5><p>Symmetry Shield Columns feature the Waters patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes and improves overall peak shape. Placing the embedded polar group close to the silica surface further reduces surface silanol activity, and the embedded polar group technology allows you to improve chromatographic peak shape and selectivity in the most challenging of method conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_HPLC_Colum_Embedded_Polar_Group_Technology_improves_chromatographic_peak_shape_and_selectivity_519288463f.jpg" alt="Waters: Symmetry HPLC Column - Embedded Polar Group Technology improves chromatographic peak shape and selectivity" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_HPLC_Colum_Embedded_Polar_Group_Technology_improves_chromatographic_peak_shape_and_selectivity_519288463f.jpg 161w," sizes="100vw" width="620" height="600"></p><h5><strong>Deliver high peptide recovery</strong></h5><p>All Symmetry300 columns are QC tested with peptide samples to ensure exceptional batch-to-batch reproducibility. These wide-pore (300 Å), reversed-phase chromatography columns deliver superior performance when simultaneously separating hydrophilic and hydrophobic peptides, neutral, and highly basic peptides. With a Symmetry300 column, you can expect better performance with either high or low mass loads.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_HPLC_Colum_Compared_to_many_competitive_100_silica_based_C18_columns_Waters_proprietary_bonding_and_end_capping_technologies_61cde5dc5b.jpg" alt="Waters: Symmetry HPLC Column - Compared to many competitive 100% silica-based C18 columns, Waters proprietary bonding and end-capping technologies" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_HPLC_Colum_Compared_to_many_competitive_100_silica_based_C18_columns_Waters_proprietary_bonding_and_end_capping_technologies_61cde5dc5b.jpg 245w," sizes="100vw" width="620" height="364"></p>

Waters Nova-Pak C18 HPLC Columns

Waters Nova-Pak C18 columns are general purpose, silica-based, reversed-phase C18 columns that are based on 4 µm particle technology

<p><strong>Nova-Pak C18 columns are general purpose, silica-based, reversed-phase C18 columns that are based on 4 µm particle technology. Compared to a 5 µm column, Nova-Pak columns offer higher resolution, more efficient separations, and faster chromatography.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Nova-Pak C18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Medium</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>60 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 7 %</li></ul><h4><strong>The Waters Nova-Pak C18 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Nova-Pak C18 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Nova-Pak C18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>Nova-Pak Columns</strong></h3><h4><strong>Scale-up from analytical to prep, simplified</strong></h4><p>Reducing solvent consumption while maintaining resolution for complex mixtures is important, but that’s a challenge with larger particle sizes. With the <strong>smaller particle sizes </strong>and <strong>shorter column lengths</strong> in Nova-Pak Columns, those problems are a thing of the past. The bonded phases of Nova-Pak Columns are available in <strong>4 µm</strong> and <strong>6 µm particle sizes</strong>, offering high resolution and faster, more efficient chromatography over columns packed with larger particle sizes.</p><p><strong>Nova-Pak Analytical Columns</strong> with <strong>4 µm particle size</strong> packing offer a range of <strong>length </strong>options with <strong>75, 100, 150, and 300 mm</strong> length steel columns. The <strong>semi-preparative</strong> <strong>Prep Nova-Pak HR Columns</strong> are packed with<strong> 6 µm particle size</strong> packings for unparalleled separation possibilities. The high-efficiency packing of Prep Nova-Pak HR Columns provides faster separations with less solvent for the added advantage of more concentrated fractions—reducing your preparative chromatography cost. To ensure batch-to-batch reproducibility, all <strong>Nova-Pak C18, C8, Cyano and Phenyl Columns</strong> are packed according to stringent quality control (QC) procedures in Waters <strong>cGMP</strong> manufacturing facility.</p><h4><strong>Overview</strong></h4><ul><li>Reduces solvent consumption while maintaining resolution for complex mixtures</li><li>Packed to stringent QC procedures to ensure batch-to-batch reproducibility</li><li>Chemistries include C18, C8, Cyano, and Phenyl stationary phases</li><li>Available in 4 µm analytical and 6 µm semi-preparative particle sizes</li><li><strong>Recommended Use:</strong> For higher resolution and faster chromatography.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Nova_Pak_Columns_fad066ad02/Waters-Nova-Pak-Columns.jpg" alt="Waters: Nova-Pak Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Nova_Pak_Columns_fad066ad02/thumbnail_Waters-Nova-Pak-Columns.jpg 245w," sizes="100vw" width="900" height="392"></p>

Waters ACQUITY UPLC C18 Columns

ACQUITY UPLC columns offer unmatched speed, resolution, and peak shape for a wide range of analytes. With advanced particle technologies and broad pH stability, they support efficient, reproducible separations in both routine and demanding applications.

<h5><strong>ACQUITY UPLC BEH C18 Columns</strong></h5><ul><li>Universal C18 column</li><li>Suitable for a diverse range of analytes</li><li>High efficiency</li><li>Industry-leading mobile phase pH 1-12 and temperature compatibility</li></ul><h5><strong>ACQUITY UPLC CSH C18 Columns</strong></h5><ul><li>Universal column choice for UV and MS applications</li><li>Suitable for a broad range of compounds</li><li>Alternate selectivity to BEH C18</li><li>Exceptional peak shape and increased load capacity</li><li>Suited for basic compounds at low pH</li></ul><h5><strong>ACQUITY UPLC HSS T3 Columns</strong></h5><ul><li>Ideal for the enhanced retention of polar and non-polar compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Mechanically stable at elevated pressures</li></ul><h5><strong>ACQUITY UPLC HSS C18 Columns</strong></h5><ul><li>General purpose silica-based C18 chemistry</li><li>Suitable for a broad range of compounds</li><li>Provides exceptional peak shapes and low pH stability</li><li>Delivers increased retention compared to hybrid organic/inorganic C18 columns</li></ul><h5><strong>ACQUITY UPLC HSS C18 SB Columns</strong></h5><ul><li>Low coverage silica-based C18 chemistry</li><li>Provides alternate selectivity for compounds impacted by silanophilic interactions</li><li>Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes</li><li>Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)</li></ul><h4><strong>ACQUITY UPLC Application-Specific Column Characteristics</strong></h4><h5><strong>ACQUITY UPLC Peptide BEH C18 Columns</strong></h5><ul><li>Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH</li><li>Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns</li><li>Two pore sizes (130 Å and 300 Å) for a wide range of peptides</li></ul><h5><strong>ACQUITY UPLC Peptide CSH C18 Columns</strong></h5><ul><li>Outstanding peak capacities with formic acid for MS-based applications</li><li>Excellent performance with TFA for optical based applications</li><li>Less dependence on TFA or FA to achieve optimal peak capacity</li><li>Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities</li><li>At low pH with the positive charge on CSH, you get less secondary interactions with the particle</li></ul><h5><strong>ACQUITY UPLC Peptide HSS T3 Columns</strong></h5><ul><li>Separations that require silica-based selectivity</li><li>Separations that necessitate increased peptide retention</li><li>Ideally suited for small, hydrophilic peptides<br>&nbsp;</li></ul><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters CORTECS T3 HPLC Columns

Waters CORTECS T3 columns are compatible with 100% aqueous mobile phases, and provide excellent retention for polar and non-polar analytes.

<p><strong>CORTECS T3 columns </strong>are <strong>compatible with 100% aqueous mobile phases</strong>, and provide <strong>excellent retention for polar and non-polar analytes.</strong> The particle morphology offers high efficnecy and lower backpressure when compared to fully porouse particles of similar particle size. <strong>CORTECS T3</strong> provides you with <strong>ultra-low column MS bleed</strong>, <strong>long lifetime</strong>s and <strong>excellent peak shape</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>120 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 4.7 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS T3 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters CORTECS Shield RP18 HPLC Columns

Waters CORTECS Shield RP18 columns provides complimentary selectivity to C8 and C18 stationary phases. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds.

<p><strong>CORTECS Shield RP18</strong> columns provides <strong>complimentary selectivity to C8 </strong>and <strong>C18</strong> stationary phases. The <strong>embedded carbamate group</strong> in the bonded phase ligand provides <strong>alternate selectivity, especially for phenolic compounds</strong> compared to straight chain alkyl columns. The solid core particle morphology offers high efficiency and lower backpressures when compared to fully porouse particles of similar particle size.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 6.4 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS Shield RP18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters CORTECS C18+ HPLC Columns

Waters CORTECS C18+ columns are general purpose, charged surface, high efficiency C18 columns based on a solid-core particle.

<p><strong>CORTECS C18+</strong> columns are general purpose, charged surface, high efficiency C18 columns based on a solid-core particle that is designed to deliver excellent peak shape for basic analytes at low pH.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>HPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 5.7 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS C18+ Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Products from category C18 HPLC columns from Waters - page 1

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