Waters ACQUITY UPLC C18 Columns

ACQUITY UPLC BEH C18 Columns

• Universal C18 column
• Suitable for a diverse range of analytes
• High efficiency
• Industry-leading mobile phase pH 1-12 and temperature compatibility

ACQUITY UPLC CSH C18 Columns

• Universal column choice for UV and MS applications
• Suitable for a broad range of compounds
• Alternate selectivity to BEH C18
• Exceptional peak shape and increased load capacity
• Suited for basic compounds at low pH

ACQUITY UPLC HSS T3 Columns

• Ideal for the enhanced retention of polar and non-polar compounds
• Compatible with 100% aqueous mobile phases
• Mechanically stable at elevated pressures

ACQUITY UPLC HSS C18 Columns

• General purpose silica-based C18 chemistry
• Suitable for a broad range of compounds
• Provides exceptional peak shapes and low pH stability
• Delivers increased retention compared to hybrid organic/inorganic C18 columns

ACQUITY UPLC HSS C18 SB Columns

• Low coverage silica-based C18 chemistry
• Provides alternate selectivity for compounds impacted by silanophilic interactions
• Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes
• Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)

ACQUITY UPLC Application-Specific Column Characteristics

ACQUITY UPLC Peptide BEH C18 Columns

• Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
• Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
• Two pore sizes (130 Å and 300 Å) for a wide range of peptides

ACQUITY UPLC Peptide CSH C18 Columns

• Outstanding peak capacities with formic acid for MS-based applications
• Excellent performance with TFA for optical based applications
• Less dependence on TFA or FA to achieve optimal peak capacity
• Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
• At low pH with the positive charge on CSH, you get less secondary interactions with the particle

ACQUITY UPLC Peptide HSS T3 Columns

• Separations that require silica-based selectivity
• Separations that necessitate increased peptide retention
• Ideally suited for small, hydrophilic peptides
   

ACQUITY UPLC Columns

Increase speed, sensitivity, and resolution, without compromising data integrity

Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.

Waters ACQUITY UPLC Columns’ sub-2-µm particle chemistries enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability.  

Overview

• Best-in-class column efficiency for superior separation speed, sensitivity, and resolution
• 8 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
• 22 stationary phases, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP
• 7 application-specific UPLC Column chemistries for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis
• More than 50 different column dimensions and configurations, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm
• Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using ACQUITY stationary phases
• Recommended Use: For improved speed, sensitivity, and resolution in methods development.

Particle technology

BEH - Ethylene Bridged Hybrid Particle Technology

ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.

Waters: BEH particle technology

CSH - Charged Surface Hybrid Particle Technology

ACQUITY UPLC CSH™ 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.

Waters: CSH particle technology

HSS - High Strength Silica (HSS) Particle Technology

ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.

Waters: HSS particle technology

Get superior peak shape for basic analytes

Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.

Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column

The ruggedness you want, the repeatability you need

ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.

Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes.

Achieve flexibility in method development

Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.

Waters: CSH Columns offer a wide range of selectivity differences (large S-values).