<div>Mulching films used in agriculture gradually break down and can become a significant source of microplastics in soils. A new study shows that these particles affect not only soil structure but also microbial life and nitrogen cycling.“We wanted to understand how microplastics from both conventional and biodegradable mulching films influence soil properties, microbial diversity and functions in real conditions,” explains Klára Šmídová, the study’s co-author . “That’s why we added realistic amounts of microplastics to soils in experimental plots in Finland, Germany, and Spain, monitored them over two growing seasons, and compared them with the control plots.”The results revealed that microplastics alter soil characteristics and microbial communities, although not always in the same way. “ However, a consistent finding was the decline in microbial activity associated with key processes, including nitrogen cycling, with negative effects increasing at higher microplastic concentrations and becoming more pronounced in the second year,” Šmídová adds. Because the microplastic levels used in the study reflect amounts already found in European soils, the findings highlight real risks to soil health. “We also found that the effects were stronger in southern than in northern Europe, likely due to differences in climate and soil conditions. This means that protecting soils from plastic pollution will require region-specific risk assessments,” she says.Over 60% of European soils are estimated to be unhealthy, with ongoing degradation driven by unsustainable land use, contamination, climate change, and extreme weather events. In response, the EU Parliament adopted the Soil Monitoring Law in October 2025, aiming to achieve healthy soils across Europe by 2050. “The law highlights the role of soil health in food security, water quality, and climate objectives, and establishes consistent soil monitoring across Member States. It includes measures to support farmers and foresters, while also creating a public list of potentially contaminated sites and an indicative watch list of emerging soil contaminants,” explains Klára Šmídová.The project brought together 19 research groups across Europe. “After each season, hundreds of samples were colle cted and sent to partners for analysis. This paper focuses on soil properties, microbial diversity, and greenhouse gas emissions. Two more manuscripts—on plastic additives and microplastic fate, and on soil invertebrates—are in preparation. Together, they will give us a complete picture of how agricultural microplastics affect soil ecosystems,” concludes Klára Šmídová.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg" alt="RECETOX: New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg 171w," sizes="100vw" width="738" height="674"><blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0269749125015866?via%3Dihub#sec2">Conventional and biodegradable agricultural microplastics: effects on soil properties and microbial functions across a European pedoclimatic gradient</a></h4>Šmídová K., Soinne H., Kim S. W., Tirronniemi J., Meffe R., Redondo-Hasselerharm P. E., Braun M., Rillig M. C., Fritze H., Adamczyk B., Nikama J., Kaseva J., Saartama V., Amelung W., Hurley R., Hofman J., Nizzetto L., Selonen S., Velmala S.Environ. Pollut. 386 (2025) 127212<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.envpol.2025.127212">10.1016/j.envpol.2025.127212</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4><div>Agricultural plastics like mulching films may become a major source of microplastic (MP) soil contamination during their degradation and fragmentation. This study investigates the effects of agricultural MPs from conventional (linear low-density polyethylene, PE) and biodegradable (starch-blended polybutylene adipate co-terephthalate, PBAT-BD) mulching films on soil physicochemical properties, aggregation, microbial diversity and functions, litter decomposition, and greenhouse gases emissions (GHG). For this purpose, MPs were mixed into soils at realistic MP concentrations of 0.005&nbsp;% and 0.05&nbsp;% (w/w) in 2022 on experimental plots in three EU countries representing different pedoclimatic conditions (Finland, Germany and Spain), followed by monitoring of the above-mentioned variables in the subsequent growing seasons 2022 and 2023. We found several significant MP-induced effects for soil properties, aggregation, microbial diversity, litter decomposition, and GHG, but the effect endpoints were less pronounced or varied considerably. Contrarily, microbial activity, contributing to soil functions such as nitrogen cycling, was consistently reduced by both conventional and biodegradable MPs. The reductions were more pronounced after the second season and for the higher MP treatment. As the higher MP concentration (i.e., 0.05&nbsp;% w/w) is environmentally relevant in Europe, our findings emphasize the potential effects of environmentally relevant MP concentrations on soil health. Furthermore, the effects increased from north to south, probably modulated by varying pedoclimatic conditions, inducing reflection of a need for regionally tailored risk</div></div>

New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

News from LabRulezLCMS Library - Week 07, 2026

Article

Application

Plastics

Environment

Science and research

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

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LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Thermo Scientific Hypersil GOLD Phenyl HPLC Columns

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns.&nbsp;A butyl (C4) linker in the phenyl bonded phase allows for superior alignment of the phenyl ring with aromatic molecules, thus enhancing pi-pi interactions and improving the retention of the molecules. The C4 linker also provides the stationary phase with moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. The outstanding peak shape results in greater sensitivity.<h5>Special selectivity</h5><ul><li>Provides selectivity complementary to that provided by alkyl chain phases</li><li>Moderate hydrophobicity compared to C18</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Silica</li><li>Stationary Phase: Phenyl/C4</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L11</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD PFP HPLC Columns

Analyze difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds with Thermo Scientific™ Hypersil GOLD™ PFP (pentafluorophenyl) HPLC columns. 

Analyze difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds with Thermo Scientific™ Hypersil GOLD™ PFP (pentafluorophenyl) HPLC columns. These columns provide an alternative selectivity to C18 columns in reversed phase applications with excellent peak shape and sensitivity.<h5>Unique selectivity</h5><ul><li>Increased retention and selectivity for positional isomers of halogenated compounds</li><li>Excellent selective analysis of non-halogenated compounds, in particular polar compounds containing hydroxyl, carboxyl, nitro, or other polar groups</li><li>Efficient separation of difficult mixtures containing closely related halogenated and polar compounds</li></ul>The introduction of a fluorine group into the stationary phase causes significant changes in solute-stationary phase interaction and increases the selectivity and extra retention.<h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 18130 psi (1250 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: PFP (Pentafluorophenylpropyl)</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L43</li><li>Carbon Load: 8%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Accucore PFP HPLC Columns

Improve LC separations of halogenated compounds and nonhalogenated polar analytes using Thermo Scientific™ Accucore™ PFP LC columns. 

Improve LC separations of halogenated compounds and nonhalogenated polar analytes using Thermo Scientific™ Accucore™ PFP LC columns. Rugged 2.6μm solid-core particles enable fast, high-resolution separations at low backpressures.Fluorine groups in the PFP stationary phase provide enhanced retention and selectivity for positional isomers of halogenated compounds and for compounds with polar functional groups. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Alternative Selectivity</h5><ul><li>Pentafluorophenyl (PFP) phase provides a unique selectivity for halogenated species and nonhalogenated polar compounds.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: PFP (Pentafluorophenylpropyl)</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L43</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Accucore Phenyl-X HPLC Columns

Achieve fast, high-resolution separations of aromatic compounds using Thermo Scientific™ Accucore™ Phenyl-X columns.

Achieve fast, high-resolution separations of aromatic compounds using Thermo Scientific™ Accucore™ Phenyl-X columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures. The phenyl-X alkyl aromatic bonded phase offers unique reversed-phase shape selectivity with high aromatic selectivity and is compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Unique Selectivity and Enhanced Retention for Aromatic Compounds</h5><ul><li>Proprietary alkyl aromatic bonded phase provides unique selectivity compared to other reversed phase materials such as C18 or hexyl.</li></ul><h5>Compatible with Highly Aqueous Mobile Phases</h5><ul><li>Specially designed bonded phase will not undergo phase collapse in highly aqueous mobile phase.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: Phenyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: N/A</li><li>Carbon Load: 6%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Syncronis Phenyl  HPLC Columns

Achieve repeatable and consistent liquid chromatography (LC) separations of aromatic compounds using Thermo Scientific™ Syncronis™ Phenyl LC Columns. 

Achieve repeatable and consistent liquid chromatography (LC) separations of aromatic compounds using Thermo Scientific™ Syncronis™ Phenyl LC Columns. High surface area silica, dense stationary phase coverage, and double end-capping minimize secondary interactions and deliver reproducible high-resolution separations. The Phenyl-bonded aromatic phase offers alternative selectivity to C18 columns and is particularly useful for optimized retention of aromatic analytes.Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.<h5>Highly Reproducible Separations</h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing</li><li>Double endcapped for extra surface coverage</li></ul><h5>Exceptional Column-to-Column Consistency</h5><ul><li>Manufactured, packed and tested in ISO-9000-accredited facilities</li><li>Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications</li><li>Enhanced automated packing process</li></ul><h5>Alternative Selectivity</h5><ul><li>Enhanced retention of aromatic compounds</li></ul><h5>Available in Three Particle Sizes</h5><ul><li>1.7μm for rapid ultrahigh-performance LC (UHPLC) separations</li><li>3μm and 5μm for more traditional HPLC analysis</li></ul><h4>Specifications</h4><ul><li>Column type: Reversed Phase</li><li>Endcapped: Yes</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Phenyl</li><li>USP Type: L11</li><li>Surface Area: 320 m2/g</li><li>Temperature: 60°C</li><li>Carbon load: 11%</li><li>Phase: Reversed Phase,</li><li>Pore Size: 100 Å</li><li>pH: 2 to 8</li></ul><h4>Syncronis HPLC and UHPLC Columns</h4><h5>Enter a new world of chromatography</h5>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.<h5>Features of Syncronis columns</h5>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:<ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Thermo Scientific Accucore Biphenyl Reversed Phase HPLC Columns

Choose Thermo Scientific™ Accucore™ Biphenyl LC Columns for fast and reliable separation of critical pairs and isomers.

Choose Thermo Scientific™ Accucore™ Biphenyl LC Columns for fast and reliable separation of critical pairs and isomers. These columns provide unique selectivity for aromatic and moderately polar analytes. Their optimized packing offers a rugged platform for a variety of matrices. Based on Core Enhanced Technology, Accucore liquid chromatography reversed phase columns provide fast, high-resolution separations without the elevated backpressures seen using sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-mode selectivity for aromatic and moderately polar analytes</li><li>Enhanced Pi-pi interactions with aromatics</li><li>Excellent lot-to-lot reproducibility</li></ul>Trust your separation of critical pairs and isomers to Accucore Biphenyl LC Columns. These columns offer fast run times, excellent robustness, and consistent reproducibility, and provide the perfect balance of polar analyte retention and hydrophobic analyte elution.<h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC/UHPLC</li><li>Max. Pressure: 8700 psi (600 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Biphenyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 6%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Thermo Scientific Accucore Phenyl-Hexyl HPLC Columns

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes</li><li>Enhanced Pi-pi Interactions with Aromatics</li><li>Moderate Hydrophobicity</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Phenyl/Hexyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Products from category Phenyl HPLC columns - page 1

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