<p><strong>Scientists at IOCB Prague are uncovering new details of gene transcription. They have identified a previously unknown molecular mechanism by which the transcription of genetic information from deoxyribonucleic acid (DNA) into ribonucleic acid (RNA) can be initiated. The researchers focused on a specific class of molecules known as alarmones, which are found in cells across a wide range of organisms and whose levels often increase under conditions of cellular stress. The results were published in the prestigious scientific journal </strong><i><strong>Nature Chemical Biology</strong></i><strong>.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Hana Cahová, head of the Chemical Biology of Nucleic Acids research group." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_bc2bfffa6c.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Molecules of ribonucleic acid can carry a variety of chemical modifications at one end, known as caps. In eukaryotic organisms, including human cells, the best-known cap plays an important role in RNA stability and in regulating its subsequent fate. In recent years, however, it has become clear that alternative, non-canonical RNA caps also exist, although their formation and the mechanisms by which they are attached to RNA remain only partially understood. These include alarmone caps, formed by dinucleoside polyphosphate molecules, that protect cellular RNA at moments when the cell is under threat.</p><p>In their study,<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://cahova.group.uochb.cz/en"><strong>Dr. Hana Cahová</strong></a> and colleagues examined bacterial RNA polymerase and investigated how this enzyme can initiate transcription using dinucleoside polyphosphates (NpNs) instead of the standard RNA building blocks. For the first time, the scientists described, at the atomic level, how RNA bearing an alarmone cap can be generated directly at the initiation of gene transcription. They also observed that NpNs bind through a different type of base pairing than is typically observed.</p><p>The work was significantly shaped by <strong>Valentina Serianni from Hana Cahová’s team</strong>, who demonstrated the ability of dinucleoside polyphosphates to initiate gene transcription, and by <strong>Jana Škerlová</strong>, who focused on the structural analysis of RNA polymerase. Using cryogenic electron microscopy data, she showed how dinucleoside polyphosphate molecules bind within the active site of RNA polymerase – that is, the core of the enzyme where genetic information is transcribed. Together, their findings help to elucidate the processes that accompany the transcription of genetic information from DNA to RNA.</p><p>Hana Cahová adds: <i>“We’re describing something that truly occurs in cells and that we’re now able to observe directly at the level of individual molecules. This allows us to answer fundamental questions about cellular processes, such as how cells adapt to stress. RNA plays a central role in this, as it carries the cascade of information underlying any cellular response – for example, to threatening conditions caused by nutrient deprivation or temperature shock.”</i></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg" alt="IOCB Prague/Tomáš Belloň: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: Dr. Tomáš Kouba, head of the Cryogenic Electron Microscopy core facility." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_2_0fe88ff9e9.jpg 234w," sizes="100vw" width="2000" height="1335"></p><div><p>Cryogenic electron microscopy (cryo-EM) was central to the project. This part of the work was led by<strong> Dr. Tomáš Kouba</strong>, one of the authors of the study. <i>“Cryogenic electron microscopy allows us to freeze biological molecules in a state very close to their natural form and then determine their three-dimensional structure. This makes it possible to look directly into the active centers of enzymes and observe their function down to the atomic level,”</i> he explains.</p><p>Last year, IOCB Prague opened a new <a target="_blank" rel="noopener noreferrer" href="https://cryoem.group.uochb.cz/en"><strong>cryo-EM center</strong></a><strong> </strong>that is unique within the Czech research environment. State-of-the-art cryogenic electron microscopes are housed in a specially designed building and enable the study of biological processes with exceptional structural precision.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg" alt="IOCB Prague: Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription: This work shows how NpnNs initiate bacterial transcription. Cryo-EM reveals how nucleobases pair with the template: one in canonical and one in reverse Watson–Crick mode, providing a structural basis for their function as non-canonical RNA caps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Vyzkum_z_UOCHB_odhaluje_dosud_nepoznany_zpusob_prepisu_geneticke_informace_3_4d881e7a58.jpg 245w," sizes="100vw" width="2000" height="1077"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41589-025-02134-5"><strong>Molecular insight into 5′ RNA capping with Np<sub>n</sub>Ns by bacterial RNA polymerase</strong></a></h4><p>Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimoněnko, Milan Fábry, Pavlína Řezáčová, Tomáš Kouba &amp; Hana Cahova&nbsp;</p><p>Nat. Chem. Biol. 21 (2026)</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41589-025-02134-5">https://doi.org/10.1038/s41589-025-02134-5</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>RNA capped with dinucleoside polyphosphates has been discovered in bacteria and eukaryotes only recently. The likely mechanism of this specific capping involves direct incorporation of dinucleoside polyphosphates by RNA polymerase as noncanonical initiating nucleotides. However, how these compounds bind into the active site of RNA polymerase during transcription initiation is unknown. Here, we explored transcription initiation in vitro, using a series of DNA templates in combination with dinucleoside polyphosphates and model RNA polymerase from <i>Thermus</i> <i>thermophilus</i>. We observed that the transcription start site can vary on the basis of the compatibility of the specific template and dinucleoside polyphosphate. Cryo-electron microscopy structures of transcription initiation complexes with dinucleoside polyphosphates revealed that both nucleobase moieties can pair with the DNA template. The first encoded nucleotide pairs in a canonical Watson–Crick manner, whereas the second nucleobase pairs noncanonically in a reverse Watson–Crick manner. Our work provides a structural explanation of how dinucleoside polyphosphates initiate RNA transcription.</p></div></div>

Research from IOCB Prague reveals a previously unknown mechanism of genetic transcription

<p><strong>Food adulteration is a phenomenon with a long history that has gained increasing importance in recent years due to the globalization of food supply chains and strong economic pressures. Whether it involves diluting olive oil with cheaper oils, adding sugar to honey, or the hidden use of substitutes in protein supplements, the result is a loss of consumer trust and, in some cases, a potential risk to health. Analytical chemistry plays a key role in this area by providing methods and technologies capable of identifying and quantifying target compounds as well as unwanted impurities in a wide range of food matrices.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg" alt="Watrex: Food Adulteration and How It Is Revealed by Analytical Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Praha_Falsovani_potravin_a_jak_ho_odhali_analyticka_chemie_6912db5492.jpg 245w," sizes="100vw" width="1600" height="900"></p><h4><strong>The Role of Chromatography in Forensic Food Analysis</strong></h4><p>Chromatography, particularly liquid chromatography (HPLC), is one of the most effective analytical tools for separating complex mixtures of molecules. It enables:</p><ul><li>qualitative identification of individual components in a sample,</li><li>quantitative determination of their concentrations,</li><li>detection of impurities and unexpected components that may indicate<br>adulteration.</li></ul><p>In practice, HPLC is often combined with various types of detectors (UV/VIS, DAD, refractive index detectors) or with other techniques (e.g., mass spectrometry) to increase selectivity and sensitivity.</p><h4><strong>Isocratic vs. Gradient Elution</strong></h4><p>In the analysis of food samples, chromatographic methods are selected according to the analytical goal:</p><ul><li><strong>Isocratic HPLC </strong>uses a constant mobile phase composition throughout the analysis. It is simple, robust, and ideal for routine determinations of well-defined target analytes (e.g., sugars or vitamins).</li><li><strong>Gradient HPLC</strong> changes the mobile phase composition during separation, enabling better resolution of complex mixtures with a wide range of polarities (e.g., peptides or phenolic compounds).</li></ul><p>Gradient methods are particularly useful when analytes have very different properties and high separation efficiency is required.</p><h4><strong>Watrex Praha and the Contribution of Modern Systems to Food Analysis</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha, s.r.o.</strong></a> is one of the leading Czech manufacturers and distributors of liquid chromatography systems and instruments, with a long tradition dating back to 1990. The company has successfully transformed from a distributor into a manufacturer of its own HPLC and related technologies, focusing on modular, robust systems suitable even for demanding analytical tasks.</p><p>Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=93&amp;search=streamline"><strong>Streamline™ product line</strong></a><strong> </strong>includes several HPLC system concepts suitable for food analysis:</p><ul><li><strong>Isocratic HPLC system</strong> – a simple yet precise configuration with a single pump, column thermostat, and UV/VIS detector; suitable for routine determination of known analytes (e.g., basic carbohydrates, food additives).</li><li><strong>Binary gradient HPLC system</strong> – equipped with two pumps enabling mobile phase gradient formation, which enhances separation performance for complex mixtures<br>(e.g., phenolic profiles of wine, components of aromatic extracts).</li><li><strong>GPC/SEC system (Gel Permeation / Size Exclusion Chromatography)</strong> – a specialized solution for the determination of macromolecules and polymers, also relevant for the analysis of high-molecular-weight components in foods (e.g., polysaccharides).</li></ul><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_catalog_2024_ba97395f92.pdf"><strong>Product catalogue HERE</strong></a></p></blockquote><p>Thanks to the modular nature of these systems, analysts can easily adapt the configuration to a specific analytical problem, from simple routine analyses to complex characterization of food samples.</p><h4><strong>Practical Examples of Adulteration Revealed by HPLC</strong></h4><ul><li><strong>Detection of olive oil dilution: </strong>HPLC can separate and quantify characteristic triacylglycerols and fatty acids; unusual patterns indicate the presence of cheaper oils.</li><li><strong>Estimation of sugar addition in honey:</strong> Isocratic HPLC with a refractive index detector can determine sugar profiles (glucose, fructose, sucrose), revealing dilution or the addition of sugar syrups.</li><li><strong>Identification of aromatic adulterants in wine:</strong> Gradient HPLC with a PDA detector can reveal phenolic and aromatic profiles that are altered by the addition of synthetic compounds.</li></ul><h4><strong>Conclusion</strong></h4><p>Food adulteration remains a current issue that requires reliable and sensitive analytical methods. Liquid chromatography, and in particular modern HPLC systems, represent one of the most powerful tools for detecting unwanted additives, impurities, and unexpected components in complex food samples. Manufacturers such as <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a> provide flexible chromatographic platforms covering a wide range of analytical tasks from simple isocratic to advanced gradient and GPC/SEC applications thereby supporting analysts in ensuring food quality and consumer protection.</p><blockquote><p>Did you find this article interesting? Follow <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky_HPLCsystemy"><strong>Watrex Praha</strong></a> on their social media (<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/company/watrex/?viewAsMember=true"><strong>LinkedIn</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/WatrexPrague"><strong>Facebook</strong></a>) for more engaging topics.</p></blockquote>

Food Adulteration and How It Is Revealed by Analytical Chemistry

<p><strong>Join us for a fascinating conversation with Dr. Subhoja Chakraborty from the University of Central Florida, whose cutting-edge research targets the global challenge of malaria drug resistance. Dr. Chakraborty shares her journey from structure-based drug design during her PhD to her recent collaborative work repurposing human kinase inhibitors as rapid, selective antimalarials against drug-resistant Plasmodium falciparum strains.</strong></p><h4><strong>In this episode, you'll learn:</strong></h4><ul><li>How structure-guided approaches reveal potential drug targets, including cysteine proteases like falcipain-2, and kinases implicated in malaria's complex life cycle.</li><li>The power of repurposing kinase inhibitors originally developed for cancer and other diseases, focusing on compound 12, which blocks hemozoin formation and protein kinase 6—key steps in parasite survival.</li><li>Lab techniques such as affinity, gel filtration, and size exclusion chromatography for recombinant protein purification—and how troubleshooting is crucial for high-yield, stable complexes.</li><li>Advances in mass spectrometry for characterizing protein interactions, validating drug targets, and mapping molecular pathways pivotal to malaria therapeutics.</li><li>Tips and lessons for new researchers facing the challenges of sample preparation, complex protocols, and working as part of a collaborative team.</li></ul><p>Dr. Chakraborty’s insights bridge the gap between basic science and translational drug discovery, offering hope for more effective, targeted malaria treatments in a world where resistance to frontline therapies threatens millions.</p><h4><strong>Referenced Papers:</strong></h4><ul><li>"Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activity" (Cell Chemical Biology, 2025)</li><li>"New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain" (BBRC, 2022)</li><li>"Structure-Based Optimization of Protease−Inhibitor Interactions to Enhance Specificity of Human Stefin‑A against Falcipain‑2 from the Plasmodium falciparum 3D7 Strain" (Biochemistry, 2023)</li></ul><p>If you’re a student, scientist, or simply passionate about advances in infectious disease research, this episode offers actionable insights, inspiring advice, and a front-row seat to innovation in combating malaria.</p><h3><strong>Video Transcription</strong></h3><h4><strong>From Structural Biology to Drug Discovery: Chromatography and Mass Spectrometry in Malaria Research</strong></h4><h5><strong>Introduction</strong></h5><p>In this discussion, Subhoja shares insights into her research journey, focusing on malaria drug discovery and the role of modern analytical techniques in biological and biomedical research. The conversation is aimed primarily at undergraduate students and early-career researchers interested in chemistry, biotechnology, and life sciences.</p><h5><strong>Research Focus: Malaria Drug Discovery and Kinase Targets</strong></h5><p>Subhoja is currently involved in malaria drug discovery research at the University of Central Florida, where she has been working since beginning her postdoctoral position two years ago. Her earlier PhD research focused on structure-based drug design in malaria, using a combination of in silico approaches, recombinant protein expression, and X-ray crystallography to determine precise three-dimensional structures of target proteins and their inhibitors.</p><p>This structural perspective provided a foundation for her current work, which centers on drug discovery through compound screening and repurposing. A recent publication from her laboratory explores the use of repurposed mammalian CDK-like kinase inhibitors as potential antimalarial agents. While such kinase inhibitors are well known in cancer and cardiovascular research, their application to malaria targets represents a novel and promising approach.</p><p>Kinases are attractive drug targets because of their central role in cell signaling and growth pathways. By targeting specific kinases involved in different stages of the malaria parasite life cycle—whether asexual growth, gametocytogenesis, or differentiation—it may be possible to significantly inhibit parasite growth and progression.</p><h5><strong>Chromatography in Protein Expression and Purification Workflows</strong></h5><p>Chromatography plays a critical role throughout Subhoja’s research. Her experience spans from basic chromatography techniques during her early training to advanced protein purification workflows using affinity chromatography, gel filtration (size exclusion chromatography), and ion exchange chromatography.</p><p>Recombinant proteins are routinely purified using affinity resins such as nickel or cobalt, followed by further purification to remove contaminants. Fast Protein Liquid Chromatography (FPLC) enables compact, stepwise purification workflows suitable for producing proteins for in vitro assays.</p><p>A recurring challenge in this process is the expression of high-molecular-weight proteins, which often accumulate in insoluble fractions or inclusion bodies. Careful selection of expression vectors, host systems, and induction conditions is therefore essential to maximize yield and solubility.</p><h5><strong>Integrating Size Exclusion Chromatography into the Workflow</strong></h5><p>Size exclusion chromatography (SEC) is typically used after affinity purification to further improve protein purity. Following elution with imidazole or glutathione, desalting is required to prevent buffer components from interfering with downstream assays. This is often achieved using desalting columns such as G-25 or PD-10.</p><p>To remove lower-molecular-weight contaminants, finer SEC media—such as Superdex or Sephacryl columns—are employed. Concentration steps using molecular-weight cut-off filters are then applied to obtain the desired protein concentration for functional studies.</p><p>These iterative purification and troubleshooting steps are a routine part of protein biochemistry and reflect the importance of methodical optimization and careful validation.</p><h5><strong>The Role of Mass Spectrometry in Biomolecular Characterization</strong></h5><p>Mass spectrometry is a key analytical tool in Subhoja’s research pipeline, particularly for target identification and validation. In both malaria and cancer research, MS is used alongside chromatography, immunoprecipitation, and pull-down assays to study protein interactions and molecular recognition events.</p><p>Affinity-based mass spectrometry enables the identification of binding partners and interactors, whether studying proteins, long non-coding RNAs, or protein complexes. Techniques such as native MS, tandem affinity purification, and proximity labeling (e.g., TurboID-based workflows) provide deeper insights into molecular networks and signaling pathways.</p><p>These approaches are essential for understanding how candidate drug targets function within complex biological systems.</p><h5><strong>Sample Preparation Challenges in Biological Research</strong></h5><p>Sample preparation remains one of the most challenging aspects of biological experimentation. Whole-cell lysates contain proteases, nucleases, and other components that can rapidly degrade target molecules. Preventing degradation requires minimizing preparation time, maintaining low temperatures, and using appropriate inhibitor cocktails.</p><p>Reproducibility is also critical: biological experiments require multiple replicates, and variability is often unavoidable. Careful handling, protocol optimization, and continuous troubleshooting are essential to ensure reliable results, whether preparing samples for chromatography, mass spectrometry, sequencing, or immunoprecipitation.</p><h5><strong>Advances in Chromatography and MS for Malaria Research</strong></h5><p>Modern advances in chromatography and mass spectrometry significantly enhance malaria research by enabling precise target identification, quantification, and functional characterization. Drug discovery workflows begin with construct design and recombinant expression, followed by purification and interaction studies.</p><p>In malaria research, stage-specific parasite biology requires careful extraction and analysis of proteins from different life cycle stages. Tandem affinity MS, immunoprecipitation, and in vivo validation studies help confirm target relevance and therapeutic potential.</p><p>Recent work on repurposed kinase inhibitors has shown promising results, including inhibition of hemozoin formation—a key detoxification pathway for the malaria parasite—highlighting the potential of such compounds for further development.</p><h5><strong>Malaria, Drug Resistance, and Global Impact</strong></h5><p>Malaria remains a major global health burden, with over 600,000 deaths annually and widespread drug resistance complicating treatment. Artemisinin resistance, particularly associated with mutations in the K13 propeller domain, has driven the search for new targets and therapies.</p><p>During her PhD, Subhoja focused on falcipain-2, a cysteine protease involved in hemoglobin degradation within the parasite. This pathway is critical for parasite survival and has emerged as a promising drug target. Her work revealed that falcipain-2 operates within a larger protein complex responsible for hemoglobin processing and amino acid release.</p><p>Understanding these molecular mechanisms is essential for developing robust, stage-specific antimalarial strategies.</p><h5><strong>Advice for Early-Career Researchers</strong></h5><p>For researchers just starting out, Subhoja emphasizes the importance of hands-on experience. Working directly with cells, lysates, and analytical tools is essential for truly understanding experimental systems. Failure is an inherent part of research, and perseverance is key.</p><p>Motivation, teamwork, careful sample handling, and thorough preparation all contribute to long-term success. Reading extensively, critically evaluating one’s own work, and appreciating incremental progress are vital aspects of a sustainable research career.</p><p>Every successful experiment—no matter how small—represents meaningful progress and contributes to the broader goals of science and medicine.</p><h5><strong>Closing Remarks</strong></h5><p>The discussion highlights how interdisciplinary approaches combining chromatography, mass spectrometry, structural biology, and biochemistry are driving advances in malaria drug discovery. Through careful experimental design, analytical rigor, and collaborative teamwork, researchers can address complex biological challenges and contribute to global health solutions.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Affinity, Gel Filtration, Size Exclusion Chromatography: Repurposing Kinase Inhibitors for Malaria

Article

Product

Video

Scientists

Food

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Thermo Scientific Dionex IonPac AS22 Fast-4μm IC Columns

Attain the optimal combination of chromatographic speed and resolution using the Thermo Scientific™ Dionex™ IonPac™ AS22-Fast-4μm column.

<p><strong>Attain the optimal combination of chromatographic speed and resolution using the Thermo Scientific™ Dionex™ IonPac™ AS22-Fast-4μm column. Designed specifically for compliance monitoring of inorganic anions in accordance with U.S. EPA Method 300.0 (A), this moderate capacity column combines the selectivity of the Dionex IonPac AS22-Fast column with fast high resolution separations. Compared to the Dionex IonPac AS22-Fast column, the new Dionex IonPac AS22-Fast-4μm column uses smaller resin particles for more efficient separations resulting in faster run times, more accurate peak integration and more reliable results.</strong></p><h5><strong>Fast separation of common inorganic anions with excellent resolution</strong></h5><ul><li>Obtain the highest peak efficiency, through the use of smaller resin particles, of common inorganic anions in diverse sample matrices</li><li>Attain fast isocratic separation of the common inorganic anions in less than 5 min</li><li>Perform compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Achieve excellent separation of inorganic anions and low-molecular weight acids in diverse sample matrices</li><li>Obtain high peak efficiency and increased peak resolution for more consistent integration and more reliable results</li><li>Transfer application easily--this 4μm column has the the same selectivity as the Dionex IonPac AS22-Fast column</li><li>Achieve excellent resolution of the carbonate peak away from the common inorganic anions</li><li>Reduce run time without loss of resolution compared to the Dionex IonPac AS22-Fast column</li><li>Faster alternative, using carbonate/bicarbonate eluents, for Dionex IonPac AS4A-SC, AS12A, AS14, AS14A and AS22 inorganic anion applications</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5><strong>Use with a high-pressure IC system</strong></h5><p>Higher resolution is achieved through the use of smaller resin particles, which can produce a column backpressure over 3000 psi. When operating the Dionex IonPac AS22-Fast-4μm column, a high-pressure IC system, such as the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/410"><strong>Thermo Scientific™ Dionex™ ICS-6000 HPIC</strong></a> system is required.</p><h5><strong>Use the format that fits your lab</strong></h5><ul><li>4x150mm, 2x150mm analytical columns</li><li>4x30mm, 2x30mm guard columns</li><li>Shorter 150mm columns facilitate faster runs while the smaller particle size maintains a higher resolution separation</li></ul><p><strong>Note: </strong><i>For simple, accurate eluent preparation, use the Dionex IonPac AS22 Eluent Concentrate or the Dionex Eluent Generator (Dionex EGC 500 K2CO3 cartridge with Dionex EPM 500 pH modifier).</i></p><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 126 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 126 μeq</li><li><strong>Column Chemistry:</strong> Carbonate/Bicarbonate</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 5000 psi (345 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> Super Macroporous Resin</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS19-4μm IC Columns

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water using the Thermo Scientific™ Dionex™ IonPac™ AS19-4μm hydroxide-selective anion-exchange column.

<p><strong>Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water using the Thermo Scientific™ Dionex™ IonPac™ AS19-4μm hydroxide-selective anion-exchange column. Its high resolution, high capacity and selectivity allow the determination of bromate in drinking water at the low-μg/L level. Compared to the previous </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2055"><strong>Dionex IonPac AS19 column</strong></a><strong>, this anion-exchange column uses smaller resin particles for more efficient separations resulting in more accurate peak integration and more reliable results.</strong></p><h5><strong>Easy Method Transfer</strong></h5><p>With similar selectivity and increased peak efficiencies, you can easily transfer applications from the Dionex IonPac AS19 column to the Dionex IonPac AS19-4μm column.</p><h5><strong>Significantly Reduce Eluent Usage</strong></h5><p>The Dionex IonPac AS19-4μm Capillary Column is packed with the same material as the equivalent standard bore version (providing the same performance as a 4mm column), but requires only 1/100th the eluent flow rate. The capillary format provides the advantage of significantly reduced eluent consumption, which helps reduce operating costs.</p><h5><strong>Dionex IonPac AS19-4μm Capillary Column Features</strong></h5><ul><li>Choose from three formats: 4×250mm, 2×250mm and 0.4×250mm</li><li>Recommended hydroxide-selective column for trace bromate in drinking water matrices</li><li>Meets or exceeds performance requirements of US EPA Method 300.0 and US EPA Method 300.1</li><li>High capacity: 240μeq per column (4×250mm)</li><li>Column selectivity is optimized for 30 °C operating temperature</li><li>Compatible with organic solvents</li></ul><p>These capillary columns require high pressure Thermo Scientific™ Dionex™ ICS-5000+ or Dionex ICS-4000 HPIC™ system to run at standard flow rates.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 240 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity: </strong>240 μeq</li><li><strong>Column Chemistry:</strong> Hydroxide</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 5000 psi (345 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS19-4 μm</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS18-Fast IC Columns

Use these shorter 150mm Thermo Scientific™ Dionex™ IonPac™ AS18-Fast columns for fast runs when monitoring inorganic anions in drinking water and wastewater samples

<p><strong>Use these shorter 150mm Thermo Scientific™ Dionex™ IonPac™ AS18-Fast columns for fast runs when monitoring inorganic anions in drinking water and wastewater samples for compliance with U.S. EPA Methods 300.0 (A) and 300.1. Dionex IonPac AS18 columns are high capacity hydroxide-selective anion-exchange columns. Having the same selectivity as the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2054"><strong>Dionex IonPac AS18 column</strong></a><strong>, the shorter format significantly reduces run times of common inorganic anions and low molecular weight organic acids in diverse sample matrices including drinking water, wastewater, leachates, foods, beverages, and pharmaceutical preparations.</strong></p><h5><strong>Analysis of Common Inorganic Anions and Low-Molecular Weight Organic Acids in Diverse Matrices</strong></h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Determination of low levels of inorganic anions in complex matrices using large-loop injections</li><li>Superior retention and quantification of fluoride, acetate, and formate</li><li>Fast separation of the common inorganic anions in 5 min in simple matrices using 150mm column</li><li>High efficiency, fast analysis use the 150mm column for increased sample throughput</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5><strong>Recommended for Inorganic Anions in Diverse Sample Matrices</strong></h5><ul><li>Source water and drinking water, municipal and industrial wastewater</li><li>Industrial cooling water and waste water, acid rain</li><li>Foods and beverages, anionic counterions in pharmaceutical preparations</li></ul><h5><strong>Multiple Formats for Application Flexibility</strong></h5><p>The shorter 150mm Dionex IonPac AS18-Fast columns produce faster runs of relatively clean matrices compared to the longer 250mm columns and are available in three formats with guard columns:</p><ul><li>4x150mm standard bore AS18-Fast columns with 4x30mm guard columns</li><li>2x150mm microbore AS18-Fast columns with 2x30mm guard columns</li><li>0.4x150mm capillary AS18-Fast columns with 0.4x35mm guard columns</li></ul><h5><strong>Capillary Columns Reduce Operating Costs from Lower Eluent Requirements</strong></h5><p>Capillary columns provide significantly reduced operating and waste disposal costs through eluent conservation. Packed with the same material, the Dionex IonPac AS18-Fast capillary columns provide the same performance as the standard bore and microbore counterparts. Operating costs are reduced because the capillary system operates at only 1% of the eluent flow rate of the standard bore system. Capillary columns are used with the Thermo Scientific™ Dionex™ ICS-4000 and ICS-5000+ Capillary systems.</p><h5><strong>Need Higher Resolution or Faster Separations?</strong></h5><p>If you need higher resolution separations of more complex sample matrices, refer to the longer 250mm Dionex™ IonPac™ AS18 Hydroxide-Selective Anion-Exchange column<br>For fastest, highest resolution separations of relatively cleaner sample matrices, refer to the small particle Dionex™ IonPac™ AS18-4µm Hydroxide-Selective Anion-Exchange column. Use of 4µm columns requires a high-pressure IC system.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 171 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 171 μeq</li><li><strong>Column Chemistry:</strong> Hydroxide</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS18-Fast</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS18-Fast-4μm IC Columns

Attain the optimal combination of chromatographic speed and resolution using the new Thermo Scientific™ Dionex™ IonPac™ AS18-Fast-4μm anion-exchange column.

<p><strong>Attain the optimal combination of chromatographic speed and resolution using the new Thermo Scientific™ Dionex™ IonPac™ AS18-Fast-4μm anion-exchange column. This high-capacity, high-efficiency column provides excellent resolution of inorganic anions in diverse sample matrices. This is the hydroxide-selective column of choice for fastest compliance monitoring of inorganic anions in drinking water and wastewater samples in accordance with U.S. EPA Methods 300.0 (A) and 300.1. Compared to the Thermo Scientific™ Dionex™ IonPac™ AS18-Fast column, the new Dionex IonPac AS18-Fast-4μm anion-exchange column uses smaller resin particles for more efficient peaks resulting in faster run times, more accurate peak integration, and more reliable results.</strong></p><h5><strong>Fastest Separation of Common Inorganic Anions with Excellent Resolution</strong></h5><p>Through the use of smaller resin particles and a shorter column format, this column offers the highest peak efficiency and best resolution of common inorganic anions for fast analysis of environmental waters.</p><h5><strong>Analysis of Common Inorganic Anions and Low Molecular Weight Organic Acids in Diverse Matrices</strong></h5><ul><li>Attain super fast isocratic separation of the common inorganic anions in less than 3 min</li><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>High peak efficiency and increased peak resolution for more consistent integration and more reliable results</li><li>Offers the same selectivity as the Dionex IonPac AS18-Fast column so applications are easily transferred to the 4μm column</li><li>Reduces run time without loss of resolution compared to the Dionex IonPac AS18-Fast column</li><li>Achieve excellent separation of inorganic anions and low-molecular weight acids in diverse sample matrices</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5><strong>Use 4μm Columns with High-Pressure IC Systems</strong></h5><p>Higher resolution is achieved through the use of smaller resin particles, which can produce a column backpressure over 3000 psi. When operating the Dionex IonPac AS18-Fast-4μm capillary column at the standard flow rate of 10μL/min, an IC system capable of operating up to 3000 psi is sufficient. However, to take advantage of the fast runs, using elevated flow rates, a high-pressure capillary IC system, such as the Thermo Scientific™ Dionex™ ICS-5000+ HPIC or the Thermo Scientific™ Dionex™ ICS-4000 HPIC is required. The Dionex ICS-5000+ HPIC system is required for standard operation of the Dionex IonPac AS18-Fast-4μm microbore and standard bore columns.</p><h5><strong>Use the Format That Fits Your Lab</strong></h5><p>Shorter 150mm AS18-Fast-4μm columns facilitate faster runs while the smaller particle size maintains a higher resolution separation and are available in 3 formats with guard columns:</p><ul><li>Use the 4x150mm standard bore column with the 4x30mm guard column</li><li>Use the 2x150mm analytical columns with the 2x30mm guard column</li><li>Use the 0.4x150mm capillary column with the 0.4x35mm guard column</li></ul><h5><strong>Capillary Columns Reduce Operating Costs from Lower Eluent Requirements</strong></h5><p>Capillary columns provide significantly reduced operating and waste disposal costs through eluent conservation. Packed with the same material, the Dionex IonPac AS18-Fast-4μm capillary columns provide the same performance as the standard bore and microbore counterparts. Operating costs are reduced because the capillary system operates at only 1% of the eluent flow rate of the standard bore system. Capillary columns are used with the Thermo Scientific™ Dionex™ ICS-4000 and ICS-5000+ Capillary systems.</p><h5><strong>Need Higher Resolution or Faster Separations?</strong></h5><ul><li>If you need higher resolution separations of more complex sample matrices, refer to the longer 250mm Dionex™ IonPac™ AS18 Hydroxide-Selective Anion-Exchange column</li><li>The shorter 150mm Dionex IonPac AS18-Fast columns produce faster runs of relatively clean matrices compared to the longer 250mm columns and operate under 3000 psi.</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 174 μeq</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Max. Pressure:</strong> 5000 psi (345 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> Super Macroporous Resin</li><li><strong>Format:</strong> Standard Bore</li></ul>

Thermo Scientific Dionex IonPac AS16-4μm IC Columns

Determine trace levels of perchlorate and other highly polarizable anions in drinking water, surface water, and ground water with Thermo Scientific Dionex IonPac AS16-4μm IC Columns

<h5><strong>Designed for simple, sensitive analysis</strong></h5><ul><li>Easily measure trace concentrations of perchlorate in drinking water, surface water, and ground water matrices using a large-loop injection</li><li>Improved efficiencies and resolution compared to the standard <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2052"><strong>Dionex IonPac AS16 column</strong></a></li><li>Easily transfer applications from the Dionex IonPac AS16 column to the Dionex IonPac AS16-4μm column due to their similar selectivity</li><li>Ultralow hydrophobicity allows for fast analysis of highly polarizable anions (thiosulfate, iodide, thiocyanate, and perchlorate) with a simple, isocratic eluent</li><li>Ideal for polyvalent anions, including polyphosphates</li></ul><h5><strong>Use with a high-pressure IC system</strong></h5><p>Higher resolution is achieved through the use of smaller resin particles, which produce a column backpressure over 3000 psi at the standard flow rates. A high-pressure IC system, such as the Thermo Scientific™ Dionex™ ICS-5000+ HPIC™ system, Dionex Integrion™ HPIC system, or the Dionex ICS-4000 Integrated Capillary HPIC system is required to operate this column under standard conditions.</p><h5><strong>Reduce operating costs with lower eluent requirements</strong></h5><p>Capillary Dionex IonPac AS16-4μm columns provide the same performance as the standard bore and microbore Dionex IonPac AS16-4μm columns because they are packed with the same material. Operating costs are reduced because the capillary system operates at only 1% of the eluent flow rate of the standard bore system, requiring less eluent and producing less waste.</p><h5><strong>Multiple Formats Available</strong></h5><ul><li>4 x 250 mm and 2 x 250 mm analytical columns</li><li>4 x 50 mm and 2 x 50 mm analytical guard columns</li><li>0.4 x 250 mm capillary columns</li><li>0.4 x 50 mm capillary guard columns</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation, and lower operating costs</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 170 μeq</li><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 170 μeq</li><li><strong>Column Chemistry:</strong> Hydroxide</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Ion Exchange Group: </strong>Alkanol Quaternary Ammonium Ion</li><li><strong>Max. Pressure:</strong> 5000 psi (345 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> Super Macroporous Resin</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS11-HC-4μm IC Columns

Achieve the optimal combination of chromatographic speed and resolution in separating organic acids and inorganic anions in complex matrices using the new Thermo Scientific Dionex IonPac AS11-HC-4μm Column. 

<p><strong>Achieve the optimal combination of chromatographic speed and resolution in separating organic acids and inorganic anions in complex matrices using the new Thermo Scientific™ Dionex™ IonPac™ AS11-HC-4μm Capillary, Analytical &amp; Guard Column. This high-capacity, high-efficiency column provides excellent resolution of a large number of organic acids and inorganic anions in complex sample matrices. Compared to the Thermo Scientific™ Dionex™ IonPac™ AS11-HC column, this column uses smaller resin particles for more efficient separations resulting in more accurate peak integration and more reliable results.</strong></p><p>Complex sample matrices such as chemical wastewater effluents and fermentation broth solutions containing a variety of inorganic anions and organic acids can be analyzed using the high resolution Dionex IonPac AS11-HC-4μm column.<strong>&nbsp;</strong></p><p><strong>Appropriate analytes include:</strong></p><ul><li>Monovalent and divalent organic acids</li><li>Organic acids and inorganic anions in complex sample matrices or uncharacterized samples</li></ul><h5><strong>Achieve Higher Resolution With High Capacity Columns</strong></h5><ul><li>Facilitates injection of more concentrated samples without column overloading or peak broadening</li></ul><h5><strong>Enjoy Higher Performance With the Smaller 4μm Resin Particle Size</strong></h5><ul><li>Easily transfer applications from the Dionex IonPac AS11-HC column to the 4μm column</li><li>Get similar selectivity as the Dionex IonPac AS11-HC column</li><li>Increased peak resolution for more consistent results</li><li>Obtain improved separation for monovalent carboxylic acids, including quinate, lactate, acetate, propionate, formate, and butyrate</li></ul><h5><strong>Use the Format That Fits Your Lab</strong></h5><p>The Dionex IonPac AS11-HC-4μm column is available in three formats: 4x250 mm, 2x250 mm, and 0.4x250mm. The capillary format provides reduced eluent consumption, which helps reduce operating costs.</p><h5><strong>Use With a High-Pressure Capillary IC System</strong></h5><p>The smaller resin particles used in the Dionex IonPac AS11-HC-4μm column produces a backpressure over 3000 psi at the standard flow rates. A high-pressure capillary IC system, such as the Thermo Scientific™ Dionex™ ICS-5000+ HPIC™ or the Dionex ICS-4000 Capillary HPIC system is required to operate this column under standard conditions.</p><h5><strong>For Best Performance</strong></h5><ul><li>Use the Thermo Scientific™ Dionex™ IonPac™ AS11 column for fast analysis of organic acids and anions in well-characterized samples</li><li>Use the Thermo Scientific™ Dionex™ IonPac™ ICE-AS1 or ICE-AS6 columns for organic acids in high-ionic-strength samples</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Anion Exchange Capacity:</strong> 290 μeq</li><li><strong>Column Capacity:</strong> 290 μeq</li><li><strong>For Use With (Application):</strong> Anion Exchange</li><li><strong>Max. Pressure:</strong> 5000 psi (345 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac AS11-HC-4μm</li><li><strong>Format:</strong> Standard Bore</li></ul>

Thermo Scientific Dionex IonPac CS10 IC Columns

Choose Thermo Scientific Dionex IonPac CS10 Cation-Exchange Columns for isocratic separation of inorganic cations using hydrochloric acid (HCl) with diaminopropionic (DAP) acid eluents. 

<p><strong>Choose Thermo Scientific™ Dionex™ IonPac™ CS10 Cation-Exchange Columns for isocratic separation of inorganic cations using hydrochloric acid (HCl) with diaminopropionic (DAP) acid eluents. Well suited to determine common inorganic cations and aliphatic and aromatic amines in diverse sample matrices, including environmental waters, power plant waters treated with ammonium, morpholine, or ethanolamine; chemical additives; chemical process solutions; scrubber solutions; plating baths; and industrial solvents.</strong></p><h5><strong>Recommended Cation-Exchange Columns by Use</strong></h5><p>Since the introduction of the Dionex IonPac CS10 column, Dionex has introduced several new cation-exchange columns with advanced features. These modern columns and their recommended uses are listed below.</p><ul><li>The <strong>Dionex IonPac CS11</strong> column has selectivity similar to the Dionex IonPac CS10 column, with improved sensitivity and lower eluent consumption due to its smaller 2 mm i.d. format.</li><li>The <strong>Dionex IonPac CS12A </strong>column improves performance for fast analysis of inorganic cations and ammonium in diverse matrices.</li><li>The <strong>Dionex IonPac CS17 </strong>column is recommended for hydrophobic and polyvalent amines.</li><li>The <strong>Dionex IonPac CS19</strong> and <strong>CS19-4μm</strong> columns are recommended for polar amines including alkanolamines and methylamines and also moderately hydrophobic amines and polyvalent amines.</li><li>Compatible with organic solvents, to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5><strong>Formats Available for Application Flexibility</strong></h5><ul><li>4x250mm analytical column</li><li>4x50mm guard column</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column Capacity:</strong> 80 μeq</li><li><strong>For Use With (Application):</strong> Cation Exchange</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac CS10</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS11 IC Columns

Choose Thermo Scientific Dionex IonPac CS11 low-capacity, sulfonate-functionalized, cation-exchange column for isocratic separation of inorganic cations using HCl with DAP eluents.

<p><strong>Choose Thermo Scientific™ Dionex™ IonPac™CS11 Analytical and Guard Columns for isocratic separation of inorganic cations using hydrochloric acid (HCl) with diaminopropionic (DAP) acid eluents. This column has selectivity similar to the IonPac CS10 column, with improved sensitivity and lower eluent consumption due to its smaller 2mm I.D. format.</strong></p><p>The Dionex IonPac CS11column can be used to determine common inorganic cations and aliphatic and aromatic amines in diverse sample matrices, however, advances in cation-exchange column technology have produced better column choices for these separations.</p><h5><strong>Recommended Cation-Exchange Columns by Use</strong></h5><p>Since the introduction of the Dionex IonPac CS11 column, Dionex has introduced several new cation-exchange columns with advanced features. For example, most modern Dionex IonPac Cation Exchange columns use simple methanesulfonic acid eluents rather than DAP-HCl eluents. These columns and their recommended uses are listed below.</p><ul><li>The <strong>Dionex IonPac CS12A </strong>column improves performance for fast analysis of inorganic cations and ammonium in diverse matrices.</li><li>The <strong>Dionex IonPac CS17 </strong>column is recommended for hydrophobic and polyvalent amines.</li><li>The <strong>Dionex IonPac CS19</strong> and <strong>CS19-4μm</strong> columns are recommended for polar amines including alkanolamines and methylamines and also moderately hydrophobic amines and polyvalent amines.</li><li>Compatible with organic solvents, to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5><strong>Formats Available for Application Flexibility</strong></h5><ul><li>2x250mm analytical column</li><li>2x50mm guard column</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Crosslinking: </strong>5% DVB</li><li><strong>Column Capacity:</strong> 35 μeq</li><li><strong>For Use With (Application):</strong> Cation Exchange</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> pH 0 to 14, 0 to 100% HPLC Solvents</li><li><strong>Stationary phase:</strong> IonPac CS11</li><li><strong>Format:</strong> Microbore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS12 IC Columns

Obtain robust, reliable results and fast isocratic separation of common inorganic cations and alkanolamines using the Thermo Scientific Dionex IonPac CS12 Cation-Exchange Column.

<p><strong>Obtain robust, reliable results and fast isocratic separation of common inorganic cations and alkanolamines using the Thermo Scientific™ Dionex™ IonPac™ CS12 Cation-Exchange Column. This is a high-capacity, cation-exchange column designed for the fast, isocratic separation of lithium, sodium, ammonium, potassium, magnesium, and calcium using methanesulfonic or sulfuric acid eluents in diverse sample matrices. However, the newer Thermo Scientific™ Dionex™ IonPac™ CS12A column provides improved performance and is the recommended column for inorganic cation analysis.</strong></p><h5><strong>Fast Isocratic Separation of Common Inorganic Cations</strong></h5><p>The Dionex IonPac CS12 Cation-Exchange Column provides fast, isocratic separation of cations in diverse sample matrices, including drinking water, power plant waters, soil extracts, acid digests, chemical additives, chemical process solutions, scrubber solutions, and plating baths. However, the Dionex IonPac CS12A column improves performance for fast analysis of inorganic cations and ammonium in diverse matrices and is the recommend column for these applications. The columns are compatible with organic solvents, excluding alcohols, to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</p><h5><strong>Multiple Formats Available for Application Flexibility</strong></h5><ul><li>4x250mm, 2x250mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 2.8 meq</li><li><strong>For Use With (Application):</strong> Cation Exchange</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> Acidic Eluents, 0 to 100% HPLC Solvent Compatible, Alcohols Should be Avoided</li><li><strong>Stationary phase:</strong> Macroporous Resin</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS12A IC Columns

Separate common cations in as little as four minutes using methanesulfonic acid (MSA) or sulfuric acid eluents with Thermo Scientific Dionex IonPac CS12A Cation-Exchange Columns.

<p><strong>Separate common cations in as little as four minutes using methanesulfonic acid (MSA) or sulfuric acid eluents with Thermo Scientific™ Dionex™ IonPac™ CS12A Cation-Exchange Columns. These medium-capacity, carboxylate-functionalized cation-exchange columns are recommended for fast, isocratic separation of lithium, sodium, ammonium, potassium, magnesium, and calcium in diverse sample matrices. The IonPac CS12A column is the recommended replacement for all IonPac CS12 column applications.</strong></p><h5><strong>Analysis of Common Cations plus Ammonium in Diverse Sample Matrices</strong></h5><ul><li>Superior fast isocratic separation of mono- and divalent cations plus ammonium in diverse, complex matrices</li><li>Isocratic separation of Group I and II cations plus ammonium in 15 minutes in diverse matrices using a 250mm column</li><li>Fast, high resolution, isocratic separation of Group I and II cations plus ammonium in 4 minutes in simple matrices using 150mm column</li><li>Higher-capacity applications use 250mm columns, allowing injection of more concentrated samples without overloading or peak broadening</li><li>High efficiency, fast analysis use the 150mm column for increased sample throughput</li><li>Dionex IonPac CS12A-MS column provides fast elution at the low flow rates required for IC-MS, making it the ideal choice for IC-MS screening</li><li>Compatible with organic solvents, excluding alcohols, to enhance analyte solubility, modify column selectivity or allow effective column cleanup</li></ul><p>The Dionex IonPac CS12A-8μm and CS12A-5μm Capillary Columns offer the same selectivity as their analytical-scale counterparts with lowering operating costs.</p><h5><strong>Recommended for Common Cations and Ammonium in Diverse Sample Matrices</strong></h5><ul><li>Source water and drinking water, municipal and industrial wastewater, soil extracts, acid digests</li><li>Industrial cooling water, scrubber solutions; plating baths; and industrial solvents</li><li>Power plant waters treated with ammonium or morpholine; chemical additives; chemical process solutions</li></ul><h5><strong>Reduce Operating Costs With Lower Eluent Requirements</strong></h5><p>The Dionex IonPac CS12A capillary columns provide the same performance as the standard bore and microbore Dionex IonPac CS12A columns because they are packed with the same material. Operating costs are reduced because the capillary system operates at only 1% of the eluent flow rate of the standard bore system, requiring less eluent and producing less waste.</p><h5><strong>Multiple Formats Available for Application Flexibility</strong></h5><ul><li>4x250mm, 2x250mm, 3x150mm or 2x100mm analytical columns</li><li>4x50mm or 2x50mm, 3x30mm guard columns</li><li>0.4x250mm, 0.4x150mm capillary columns</li><li>0.4x50mm or 0.4x35mm capillary guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Shorter 150mm and 100mm columns produce faster runs of relatively clean matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Crosslinking: </strong>55% DVB</li><li><strong>Column Capacity:</strong> 2800 μeq</li><li><strong>For Use With (Application):</strong> Cation Exchange</li><li><strong>Max. Pressure:</strong> 4000 psi (275 bar)</li><li><strong>Solvent Compatibility:</strong> Acidic Eluents, 0 to 100% Acetonitrile, Alcohols Should be Avoided</li><li><strong>Stationary phase:</strong> IonPac CS12A-8μm</li><li><strong>Format:</strong> Standard Bore</li><li><strong>Packing Material: </strong>Ethylvinylbenzene/Divinylbenzene</li></ul>

Products from category Ion Chromatography Columns (IC) - page 2

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