Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

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Science and research

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Watrex P280 HPLC Pump Prep, stainless steel

Explore the future of preparative chromatography with PrecisionPrep™ P280, a state-of-the-art pump that redefines efficiency and precision.

Explore the future of preparative chromatography with PrecisionPrep™ P280, a state-of-the-art pump that redefines efficiency and precision.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>Featuring a unique parallel double-piston arrangement, this preparative chromatography pump is designed to meet the demands of high-flow applications while ensuring a stable and precise flow profile.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg" alt="Watrex P280 HPLC Pump Prep, stainless steel.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg 235w," sizes="100vw" width="1024" height="681"><h3>PUMP FEATURES</h3><h5>Parallel Double-Piston Arrangement:</h5><ul><li>The innovative dual-piston design allows for parallel operation, optimizing flow distribution and enhancing efficiency.</li><li>Experience enhanced performance and productivity in preparative chromatography with this cutting-edge arrangement.</li><li>Achieve unmatched versatility with flow rates reaching up to 80 ml/min.</li><li>Tailor the pump’s performance to accommodate a wide range of preparative chromatography applications.</li></ul><h5>Stable and Precise Flow Profile:</h5><ul><li>Enjoy a stable and precise flow profile, critical for reproducibility in preparative separations.</li><li>The P280 ensures consistent and reliable results in every run, minimizing experimental variability.</li></ul><h5>User-Friendly Interface:</h5><ul><li>The user-friendly interface simplifies operation, allowing for seamless control and monitoring of preparative chromatography processes.</li><li>Easily program and adjust parameters to suit the specific requirements of your purification protocols.</li></ul><h5>Applications:</h5><ul><li>Purification of Pharmaceuticals</li><li>Isolation of Natural Products</li><li>Large-scale Biomolecule Purification</li><li>Industrial Separations/li&gt;</li></ul><h5>Reliability Beyond Measure:</h5><ul><li>PrecisionPrep™ P280 is engineered for durability and reliability in demanding preparative chromatography applications.</li><li>Trust in its robust construction and precise engineering to deliver consistent results run after run.</li></ul><h4>Conclusion</h4>Elevate your preparative chromatography to new heights with PrecisionPrep™ P280. With a parallel double-piston arrangement, high flow rates, and an efficient pulse dampener, this pump is designed to meet the challenges of large-scale separations while ensuring a stable and precise flow profile. Choose PrecisionPrep™ for unparalleled efficiency and reliability in preparative chromatography.<h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 80 ml/min with step 0.1 ml/min</li><li>Pressure: 5 MPa/725 psi</li><li>Flow precision: ± 0,2% RSD @ 10mL/min</li><li>Flow accuracy: ± 1% @ 10mL/min</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA</li><li>Pulsation: better than ± 1% @ 10mL/min</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p280-pump-prep-stainless-steel/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex P280 HPLC Pump Prep, PEEK, biocompatible

Explore the future of preparative chromatography with PrecisionPrep™ P280, a state-of-the-art pump that redefines efficiency and precision.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>Featuring a unique parallel double-piston arrangement, this preparative chromatography pump is designed to meet the demands of high-flow applications while ensuring a stable and precise flow profile.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg" alt="Watrex P280 HPLC Pump Prep, PEEK, biocompatible" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg 208w," sizes="100vw" width="1024" height="768"><h3>PUMP FEATURES</h3><h5>Parallel Double-Piston Arrangement:</h5><ul><li>The innovative dual-piston design allows for parallel operation, optimizing flow distribution and enhancing efficiency.</li><li>Experience enhanced performance and productivity in preparative chromatography with this cutting-edge arrangement.</li><li>Achieve unmatched versatility with flow rates reaching up to 80 ml/min.</li><li>Tailor the pump’s performance to accommodate a wide range of preparative chromatography applications.</li></ul><h5>Stable and Precise Flow Profile:</h5><ul><li>Enjoy a stable and precise flow profile, critical for reproducibility in preparative separations.</li><li>The P280 ensures consistent and reliable results in every run, minimizing experimental variability.</li></ul><h5>User-Friendly Interface:</h5><ul><li>The user-friendly interface simplifies operation, allowing for seamless control and monitoring of preparative chromatography processes.</li><li>Easily program and adjust parameters to suit the specific requirements of your purification protocols.</li></ul><h5>Applications:</h5><ul><li>Purification of Pharmaceuticals</li><li>Isolation of Natural Products</li><li>Large-scale Biomolecule Purification</li><li>Industrial Separations/li&gt;</li></ul><h5>Reliability Beyond Measure:</h5><ul><li>PrecisionPrep™ P280 is engineered for durability and reliability in demanding preparative chromatography applications.</li><li>Trust in its robust construction and precise engineering to deliver consistent results run after run.</li></ul><h4>Conclusion</h4>Elevate your preparative chromatography to new heights with PrecisionPrep™ P280. With a parallel double-piston arrangement, high flow rates, and an efficient pulse dampener, this pump is designed to meet the challenges of large-scale separations while ensuring a stable and precise flow profile. Choose PrecisionPrep™ for unparalleled efficiency and reliability in preparative chromatography.<h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 80 ml/min with step 0.1 ml/min</li><li>Pressure: 5 MPa/725 psi</li><li>Flow precision: ± 0,2% RSD @ 10mL/min</li><li>Flow accuracy: ± 1% @ 10mL/min</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA</li><li>Pulsation: better than ± 1% @ 10mL/min</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p280-pump-prep-peek-biocompatible/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex P224 HPLC Pump, dual head, PEEK, biocompatible

WATREX P224 semiprep chromatography pump is a versatile and reliable tool commonly employed in laboratory settings, particularly in applications requiring precise dosing.

WATREX P224 semiprep chromatography pump is a versatile and reliable tool commonly employed in laboratory settings, particularly in applications requiring precise dosing.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>This pump is designed for semipreparative chromatography, which involves the separation and purification of compounds at a larger scale than analytical chromatography but smaller than preparative chromatography. The stainless steel version of the pump offers durability and chemical resistance, making it suitable for use with a wide range of solvents and samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg" alt="Watrex: P224 HPLC Pump, dual head, PEEK, biocompatible" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P102_HPLC_pump_PEEK_5321aceaa0.jpg 208w," sizes="100vw" width="1024" height="768"><h3>PUMP FEATURES</h3><ul><li>Flow Rate Control: P224 allows for precise control of flow rates, crucial for achieving accurate and reproducible results in chromatographic separations.</li><li>Pressure Control: P224 has a built-in pressure control system to maintain consistent pressure throughout the chromatographic process, ensuring optimal column performance.</li><li>Gradient Capability: Two or up to four P224 pumps offer gradient capabilities, allowing for the creation of complex elution profiles controlled from PC to enhance separation efficiency.</li><li>User-Friendly Interface: An intuitive and user-friendly interface facilitates easy operating and monitoring of the pump parameters, contributing to efficient workflow in the laboratory.</li><li>Compatibility: P224 is compatible with a variety of chromatography instruments and stationary phases commonly used in semipreparative applications.</li><li>Reliability and Durability: A robust construction and high-quality materials ensure the pump’s durability and reliability, minimizing downtime and maintenance requirements.</li><li>Precision in Dosing: P224 design prioritizes accuracy in dosing, making it suitable for applications where precise control over sample injection and elution volumes is essential.</li><li>Safety Features: Incorporation of safety features such as pressure sensors, and overpressure protection ensures the well-being of both the operator and the equipment.</li><li>Maintenance and Calibration: P224 pumps are easy to maintain and calibrate, with clear documentation provided by us for routine upkeep and troubleshooting.</li><li>Versatility: P224 supports a wide range of solvents and mobile phases commonly used in chromatography, offering flexibility for various applications.</li><li>Data Logging and Connectivity: The capability to log service data enhances operation traceability and documentation.</li></ul><h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 25,00 ml/min with step 0.1 ml/min (semiprep)</li><li>Pressure: 30 MPa/4300 psi</li><li>Flow precision: &nbsp;&nbsp;&nbsp;&nbsp;± 0,2% RSD @ 1mL/min. and 7 Mpa</li><li>Flow accuracy: ± 1% @ 1mL/min. and 7 Mpa</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA @ 7 Mpa</li><li>Pulsation: better than ± 0.5% @ 1mL/min. and 7 MPa</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p224-pump-dual-head-peek-biocompatible/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Watrex P224 HPLC Pump, dual head, Stainless Steel

WATREX P224 semiprep chromatography pump is a versatile and reliable tool commonly employed in laboratory settings, particularly in applications requiring precise dosing.<blockquote>Price: 4 985<bdi>&nbsp;€ ex VAT</bdi></blockquote>This pump is designed for semipreparative chromatography, which involves the separation and purification of compounds at a larger scale than analytical chromatography but smaller than preparative chromatography. The stainless steel version of the pump offers durability and chemical resistance, making it suitable for use with a wide range of solvents and samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg" alt="Watrex: P224 HPLC Pump, dual head, Stainless Steel" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_P280_HPLC_Pump_Prep_stainless_steel_b864d583c2.jpg 235w," sizes="100vw" width="1024" height="681"><h3>PUMP FEATURES</h3><ul><li>Flow Rate Control: P224 allows for precise control of flow rates, crucial for achieving accurate and reproducible results in chromatographic separations.</li><li>Pressure Control: P224 has a built-in pressure control system to maintain consistent pressure throughout the chromatographic process, ensuring optimal column performance.</li><li>Gradient Capability: Two or up to four P224 pumps offer gradient capabilities, allowing for the creation of complex elution profiles controlled from PC to enhance separation efficiency.</li><li>User-Friendly Interface: An intuitive and user-friendly interface facilitates easy operating and monitoring of the pump parameters, contributing to efficient workflow in the laboratory.</li><li>Compatibility: P224 is compatible with a variety of chromatography instruments and stationary phases commonly used in semipreparative applications.</li><li>Reliability and Durability: A robust construction and high-quality materials ensure the pump’s durability and reliability, minimizing downtime and maintenance requirements.</li><li>Precision in Dosing: P224 design prioritizes accuracy in dosing, making it suitable for applications where precise control over sample injection and elution volumes is essential.</li><li>Safety Features: Incorporation of safety features such as pressure sensors, and overpressure protection ensures the well-being of both the operator and the equipment.</li><li>Maintenance and Calibration: P224 pumps are easy to maintain and calibrate, with clear documentation provided by us for routine upkeep and troubleshooting.</li><li>Versatility: P224 supports a wide range of solvents and mobile phases commonly used in chromatography, offering flexibility for various applications.</li><li>Data Logging and Connectivity: The capability to log service data enhances operation traceability and documentation.</li></ul><h3>TECHNICAL SPECIFICATION</h3><ul><li>Flow Rates: 0,1 ml/min – 25,00 ml/min with step 0.1 ml/min (semiprep)</li><li>Pressure: 30 MPa/4300 psi</li><li>Flow precision: &nbsp;&nbsp;&nbsp;&nbsp;± 0,2% RSD @ 1mL/min. and 7 Mpa</li><li>Flow accuracy: ± 1% @ 1mL/min. and 7 Mpa</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA @ 7 Mpa</li><li>Pulsation: better than ± 0.5% @ 1mL/min. and 7 MPa</li><li>Remote control: USB</li><li>I/O connector: Start, Stop, Status, Error</li></ul>You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/pumps/preparative-pumps/p224-pump-dual-head-stainless-steel/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Products from category HPLC components focused on PrepLC distributed by WATREX Prague - page 1

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Current sensors are not good enough. Scientists propose a new way to monitor plant health

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Oligos Made Easy - Part 2: AEX