Per- and polyfluoroalkyl substances (PFAS) have become a major global concern due to their persistence, bioaccumulation, and potential health impacts. With thousands of known PFAS compounds, and new variants continuing to emerge, identifying and quantifying them across complex environmental and biological samples is an ongoing analytical challenge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg" alt="Thermo Fisher Scientific: From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg 245w," sizes="100vw" width="1024" height="574"><h3>Why PFAS analysis matters</h3>PFAS are used in everything from firefighting foams to non-stick coatings, yet their chemical stability means they persist in the environment and accumulate in living organisms. Regulators and scientists around the world are racing to better understand their distribution, fate, and toxicity.But with tens of thousands of PFAS, many without available reference standards, traditional targeted analysis alone isn’t enough. Analysts increasingly rely on non-targeted analysis (NTA) workflows powered by high-resolution accurate-mass (HRAM) mass spectrometry and advanced software to uncover the full picture.<h3>Addressing the challenges of PFAS identification</h3>Successful non-targeted PFAS analysis requires overcoming several analytical hurdles:<ol><li>Acquiring high-quality MS¹ and MS² data with ultra-high mass resolution and accuracy.</li><li>Detecting low-abundance PFAS hidden within thousands of background compounds.</li><li>Leveraging comprehensive spectral libraries and databases for confident identification.</li><li>Applying data filtering and scoring tools to assign confidence levels to annotations.</li><li>Using visualization and statistical tools to interpret and compare complex datasets.</li></ol><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer 3.5 software </a>brings together all the components needed for robust PFAS identification—integrating <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM Orbitrap MS</a> data with an expanded suite of PFAS-specific libraries and databases.In a single workflow, users can:<ul><li>Detect and annotate compounds automatically from LC-MS data.</li><li>Determine possible elemental formulas using isotope pattern and accurate mass information.</li><li>Compare MS² spectra against <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, NIST 2023, and in silico PFAS libraries.</li><li>Cross-reference annotations with the EPA PFAS Structure List and the NIST Suspect List of PFAS.</li><li>Use PFAS-specific fragment and neutral loss databases to strengthen identification confidence.</li></ul>With these resources combined, the software enables confident annotation of more than 40,000 PFAS compounds!<h3>New tools for confident, streamlined PFAS identification</h3>Compound Discoverer software introduces several new features designed to simplify and accelerate PFAS NTA workflows:<ul><li>Apply PFAS Scoring Node: Automatically calculates PFAS-specific parameters and a new PFAS Score—no R scripting required.</li><li>PFAS Plot: A visual tool that uses mass-defect metrics to highlight probable PFAS in complex datasets, following methods from Kaufman et al. (2022).</li><li>Expanded suite of libraries and databases: Combines mzCloud, NIST, in silico, PFAS fragment databases, and a new neutral loss database for greater confidence.</li><li>Advanced data visualization: Tools like Kendrick Mass Defect and Molecular Network plots reveal homologous PFAS series and related compound families.</li><li>Built-in statistical analysis tools: Compare PFAS composition between samples using Principal Component Analysis, Differential analysis and Hierarchical Cluster Analysis.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg" alt="Thermo Fisher Scientific From Complexity to Clarity Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg 245w," sizes="100vw" width="800" height="395"><h3>From complexity to clarity</h3>By uniting Orbitrap HRAM data with the most comprehensive PFAS libraries available, Compound Discoverer 3.5 software streamlines non-targeted PFAS analysis—helping laboratories move from data complexity to confident chemical clarity.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5612">Watch the on-demand webinar</a> to see the full workflow in action and learn how Compound Discoverer software can enhance your PFAS analysis.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #CompoundDiscoverer #SpectralLibraries</li></ul><blockquote><h5>Zoe Julian</h5>Zoe Julian is a Product Marketing Manager for Chromatography and Mass Spectrometry software at Thermo Fisher Scientific. With over 12 years' experience of working with chromatography and mass spectrometry techniques with a background in GC and GC-MS(/MS) organic environmental analysis, she has helped many laboratories and industries improve their workflows through a unique understanding of their challenges. Transitioning to a focus on Chromeleon CDS she has shown customers how software solutions can improve their productivity and support their day-to-day operations.</blockquote>

From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software

<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

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Thermo Scientific Dionex IonPac AS9-SC IC Columns

Separate inorganic anions and oxyhalides in simple matrices using an isocratic carbonate/bicarbonate eluent with the Thermo Scientific™ Dionex™ IonPac™ AS9-SC Carbonate Eluent Anion-Exchange Column.

Separate inorganic anions and oxyhalides in simple matrices using an isocratic carbonate/bicarbonate eluent with the Thermo Scientific™ Dionex™ IonPac™ AS9-SC Carbonate Eluent Anion-Exchange Column. The Dionex IonPac AS9-SC column is specified in US EPA Method 300.0 (B), and meets or exceeds the performance requirements of this method. However, the advanced, high-capacity Dionex IonPac AS23 Carbonate Eluent Anion-Exchange column is recommended for determination of oxyhalides and inorganic anions, such as trace bromate, in ozonated drinking water.<h5>Fast Separation of Inorganic Anion and Oxyhalides in Simple Matrices</h5><ul><li>Compliance monitoring of common anions and oxyhalides in drinking water as specified in U.S. EPA Methods 300.0 (B)</li><li>Simple, isocratic separation of inorganic anions in less than 10 minutes in drinking water</li><li>Fast isocratic separation of inorganic anions and oxyhalides in ozonated drinking water matrices</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li></ul><h5>Formats Available for Application Flexibility</h5><ul><li>4x250mm analytical columns</li><li>4x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li></ul><h4>Specifications</h4><ul><li>Column Capacity: 30 to 35 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Separation of Oxyhalides and Inorganic Anions, Anion Exchange</li><li>Ion Exchange Group: Alkyl Quaternary Ammonium</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 2 to 11, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS9-SC</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS23-4μm IC Columns

Analyze trace bromate and other oxyhalides in drinking water, ground water, wastewater, and other diverse matrices using the Thermo Scientific™ Dionex™ IonPac™ AS23-4μm columns. 

Analyze trace bromate and other oxyhalides in drinking water, ground water, wastewater, and other diverse matrices using the Thermo Scientific™ Dionex™ IonPac™ AS23-4μm columns. The high capacity and selectivity of this column facilitates the determination of trace bromate at low-μg/L levels in the presence of high amounts of chloride, sulfate, and carbonate. Compared to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2058">Dionex IonPac AS23 column</a>, the new Dionex IonPac AS23-4μm columns use smaller resin particles for more efficient separations resulting in more accurate peak integration and more reliable results.<h5>Designed Specifically for Trace Bromate Analysis in Drinking Water</h5><ul><li>Use the Dionex IonPac AS23-4μm for diverse matrices including drinking water, ground water, and waste water.</li><li>High capacity allows routine drinking water analysis without sample pretreatment or preconcentration.</li><li>Improved efficiencies and resolution compared to the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2058">Dionex IonPac AS23 column</a>.</li><li>Easily transfer applications from the Dionex IonPac AS23 column to the AS23-4μm column due to the similar selectivity.</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, and facilitate effective column clean-up.</li></ul><h5>Use With a High-Pressure IC System</h5>Higher resolution is achieved through the use of smaller resin particles, which produce a column backpressure over 3000psi at the standard flow rates. A high-pressure IC system, such as the Thermo Scientific™ Dionex™ ICS-5000+ HPIC™ or the Thermo Scientific™ Dionex™ Integrion™ HPIC™ system is required to operate this column under standard conditions and take advantage of elevated flow rates and faster run times.<h5>Multiple Formats Available</h5><ul><li>4x250mm and 2x250mm analytical columns</li><li>4x50mm and 2x50mm guard columns</li></ul>Note: Use the Dionex AS23 Eluent Concentrate for simple, accurate eluent preparation.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 320 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 320 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS23-4μm</li><li>Format: Standard Bore</li><li>Packing material: Polyvinylbenzyl Ammonium Polymer/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS22 Fast IC Columns

Thermo Scientific™ Dionex™ IonPac™ AS22-Fast column combines the selectivity of the Dionex IonPac AS22 column with super-fast separation times. 

Designed specifically for compliance monitoring of inorganic anions in accordance with U.S. EPA Methods 300.0 (A), the Thermo Scientific™ Dionex™ IonPac™ AS22-Fast column combines the selectivity of the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2057">Dionex IonPac AS22 column</a> with super-fast separation times. With the Dionex IonPac AS22-Fast column, you can quickly and simply separate common inorganic anions from a variety of sample matrices including drinking water, wastewater, process streams, and scrubber solutions.<h5>Super Fast Analysis of Inorganic Anions and Low Molecular Weight Organic Acids in Diverse Matrices</h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Super-fast isocratic separation of common inorganic anions in less than 5 min in diverse matrices such as drinking water, surface water, groundwater, and wastewater</li><li>Sufficient capacity to maintain resolution even in a short column format, which helps laboratories achieve higher productivity and increased throughput</li><li>Excellent resolution of the carbonate peak away from the common inorganic anions</li><li>Faster alternative, using carbonate/bicarbonate eluents, for Dionex IonPac AS4A-SC, AS12A, AS14, AS14A and AS22 inorganic anion applications.</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li></ul><h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4x150mm, 2x150mm analytical columns</li><li>4x30mm, 2x30mm guard columns</li><li>Shorter 150mm columns produce faster runs of relatively clean matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul>Note: For simple, accurate eluent preparation, use the Dionex IonPac AS22 Eluent Concentrate.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 126 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 126 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 3000 psi (207 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS22-Fast</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS22 Fast-4μm IC Columns

Attain the optimal combination of chromatographic speed and resolution using the Thermo Scientific™ Dionex™ IonPac™ AS22-Fast-4μm column.

Attain the optimal combination of chromatographic speed and resolution using the Thermo Scientific™ Dionex™ IonPac™ AS22-Fast-4μm column. Designed specifically for compliance monitoring of inorganic anions in accordance with U.S. EPA Method 300.0 (A), this moderate capacity column combines the selectivity of the Dionex IonPac AS22-Fast column with fast high resolution separations. Compared to the Dionex IonPac AS22-Fast column, the new Dionex IonPac AS22-Fast-4μm column uses smaller resin particles for more efficient separations resulting in faster run times, more accurate peak integration and more reliable results.<h5>Fast separation of common inorganic anions with excellent resolution</h5><ul><li>Obtain the highest peak efficiency, through the use of smaller resin particles, of common inorganic anions in diverse sample matrices</li><li>Attain fast isocratic separation of the common inorganic anions in less than 5 min</li><li>Perform compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Achieve excellent separation of inorganic anions and low-molecular weight acids in diverse sample matrices</li><li>Obtain high peak efficiency and increased peak resolution for more consistent integration and more reliable results</li><li>Transfer application easily--this 4μm column has the the same selectivity as the Dionex IonPac AS22-Fast column</li><li>Achieve excellent resolution of the carbonate peak away from the common inorganic anions</li><li>Reduce run time without loss of resolution compared to the Dionex IonPac AS22-Fast column</li><li>Faster alternative, using carbonate/bicarbonate eluents, for Dionex IonPac AS4A-SC, AS12A, AS14, AS14A and AS22 inorganic anion applications</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5>Use with a high-pressure IC system</h5>Higher resolution is achieved through the use of smaller resin particles, which can produce a column backpressure over 3000 psi. When operating the Dionex IonPac AS22-Fast-4μm column, a high-pressure IC system, such as the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/410">Thermo Scientific™ Dionex™ ICS-6000 HPIC</a> system is required.<h5>Use the format that fits your lab</h5><ul><li>4x150mm, 2x150mm analytical columns</li><li>4x30mm, 2x30mm guard columns</li><li>Shorter 150mm columns facilitate faster runs while the smaller particle size maintains a higher resolution separation</li></ul>Note: For simple, accurate eluent preparation, use the Dionex IonPac AS22 Eluent Concentrate or the Dionex Eluent Generator (Dionex EGC 500 K2CO3 cartridge with Dionex EPM 500 pH modifier).<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 126 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 126 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: Super Macroporous Resin</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS19-4μm IC Columns

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water using the Thermo Scientific™ Dionex™ IonPac™ AS19-4μm hydroxide-selective anion-exchange column.

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water using the Thermo Scientific™ Dionex™ IonPac™ AS19-4μm hydroxide-selective anion-exchange column. Its high resolution, high capacity and selectivity allow the determination of bromate in drinking water at the low-μg/L level. Compared to the previous <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2055">Dionex IonPac AS19 column</a>, this anion-exchange column uses smaller resin particles for more efficient separations resulting in more accurate peak integration and more reliable results.<h5>Easy Method Transfer</h5>With similar selectivity and increased peak efficiencies, you can easily transfer applications from the Dionex IonPac AS19 column to the Dionex IonPac AS19-4μm column.<h5>Significantly Reduce Eluent Usage</h5>The Dionex IonPac AS19-4μm Capillary Column is packed with the same material as the equivalent standard bore version (providing the same performance as a 4mm column), but requires only 1/100th the eluent flow rate. The capillary format provides the advantage of significantly reduced eluent consumption, which helps reduce operating costs.<h5>Dionex IonPac AS19-4μm Capillary Column Features</h5><ul><li>Choose from three formats: 4×250mm, 2×250mm and 0.4×250mm</li><li>Recommended hydroxide-selective column for trace bromate in drinking water matrices</li><li>Meets or exceeds performance requirements of US EPA Method 300.0 and US EPA Method 300.1</li><li>High capacity: 240μeq per column (4×250mm)</li><li>Column selectivity is optimized for 30 °C operating temperature</li><li>Compatible with organic solvents</li></ul>These capillary columns require high pressure Thermo Scientific™ Dionex™ ICS-5000+ or Dionex ICS-4000 HPIC™ system to run at standard flow rates.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 240 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 240 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS19-4 μm</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac CS10 IC Columns

Choose Thermo Scientific Dionex IonPac CS10 Cation-Exchange Columns for isocratic separation of inorganic cations using hydrochloric acid (HCl) with diaminopropionic (DAP) acid eluents. 

Choose Thermo Scientific™ Dionex™ IonPac™ CS10 Cation-Exchange Columns for isocratic separation of inorganic cations using hydrochloric acid (HCl) with diaminopropionic (DAP) acid eluents. Well suited to determine common inorganic cations and aliphatic and aromatic amines in diverse sample matrices, including environmental waters, power plant waters treated with ammonium, morpholine, or ethanolamine; chemical additives; chemical process solutions; scrubber solutions; plating baths; and industrial solvents.<h5>Recommended Cation-Exchange Columns by Use</h5>Since the introduction of the Dionex IonPac CS10 column, Dionex has introduced several new cation-exchange columns with advanced features. These modern columns and their recommended uses are listed below.<ul><li>The Dionex IonPac CS11 column has selectivity similar to the Dionex IonPac CS10 column, with improved sensitivity and lower eluent consumption due to its smaller 2 mm i.d. format.</li><li>The Dionex IonPac CS12A column improves performance for fast analysis of inorganic cations and ammonium in diverse matrices.</li><li>The Dionex IonPac CS17 column is recommended for hydrophobic and polyvalent amines.</li><li>The Dionex IonPac CS19 and CS19-4μm columns are recommended for polar amines including alkanolamines and methylamines and also moderately hydrophobic amines and polyvalent amines.</li><li>Compatible with organic solvents, to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5>Formats Available for Application Flexibility</h5><ul><li>4x250mm analytical column</li><li>4x50mm guard column</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li></ul><h4>Specifications</h4><ul><li>Column Capacity: 80 μeq</li><li>For Use With (Application): Cation Exchange</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac CS10</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS11 IC Columns

Choose Thermo Scientific Dionex IonPac CS11 low-capacity, sulfonate-functionalized, cation-exchange column for isocratic separation of inorganic cations using HCl with DAP eluents.

Choose Thermo Scientific™ Dionex™ IonPac™CS11 Analytical and Guard Columns for isocratic separation of inorganic cations using hydrochloric acid (HCl) with diaminopropionic (DAP) acid eluents. This column has selectivity similar to the IonPac CS10 column, with improved sensitivity and lower eluent consumption due to its smaller 2mm I.D. format.The Dionex IonPac CS11column can be used to determine common inorganic cations and aliphatic and aromatic amines in diverse sample matrices, however, advances in cation-exchange column technology have produced better column choices for these separations.<h5>Recommended Cation-Exchange Columns by Use</h5>Since the introduction of the Dionex IonPac CS11 column, Dionex has introduced several new cation-exchange columns with advanced features. For example, most modern Dionex IonPac Cation Exchange columns use simple methanesulfonic acid eluents rather than DAP-HCl eluents. These columns and their recommended uses are listed below.<ul><li>The Dionex IonPac CS12A column improves performance for fast analysis of inorganic cations and ammonium in diverse matrices.</li><li>The Dionex IonPac CS17 column is recommended for hydrophobic and polyvalent amines.</li><li>The Dionex IonPac CS19 and CS19-4μm columns are recommended for polar amines including alkanolamines and methylamines and also moderately hydrophobic amines and polyvalent amines.</li><li>Compatible with organic solvents, to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5>Formats Available for Application Flexibility</h5><ul><li>2x250mm analytical column</li><li>2x50mm guard column</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4>Specifications</h4><ul><li>Crosslinking: 5% DVB</li><li>Column Capacity: 35 μeq</li><li>For Use With (Application): Cation Exchange</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac CS11</li><li>Format: Microbore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS12 IC Columns

Obtain robust, reliable results and fast isocratic separation of common inorganic cations and alkanolamines using the Thermo Scientific Dionex IonPac CS12 Cation-Exchange Column.

Obtain robust, reliable results and fast isocratic separation of common inorganic cations and alkanolamines using the Thermo Scientific™ Dionex™ IonPac™ CS12 Cation-Exchange Column. This is a high-capacity, cation-exchange column designed for the fast, isocratic separation of lithium, sodium, ammonium, potassium, magnesium, and calcium using methanesulfonic or sulfuric acid eluents in diverse sample matrices. However, the newer Thermo Scientific™ Dionex™ IonPac™ CS12A column provides improved performance and is the recommended column for inorganic cation analysis.<h5>Fast Isocratic Separation of Common Inorganic Cations</h5>The Dionex IonPac CS12 Cation-Exchange Column provides fast, isocratic separation of cations in diverse sample matrices, including drinking water, power plant waters, soil extracts, acid digests, chemical additives, chemical process solutions, scrubber solutions, and plating baths. However, the Dionex IonPac CS12A column improves performance for fast analysis of inorganic cations and ammonium in diverse matrices and is the recommend column for these applications. The columns are compatible with organic solvents, excluding alcohols, to enhance analyte solubility, modify column selectivity, or allow effective column cleanup<h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4x250mm, 2x250mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4>Specifications</h4><ul><li>Crosslinking: 55% DVB</li><li>Column Capacity: 2.8 meq</li><li>For Use With (Application): Cation Exchange</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: Acidic Eluents, 0 to 100% HPLC Solvent Compatible, Alcohols Should be Avoided</li><li>Stationary phase: Macroporous Resin</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS12A IC Columns

Separate common cations in as little as four minutes using methanesulfonic acid (MSA) or sulfuric acid eluents with Thermo Scientific Dionex IonPac CS12A Cation-Exchange Columns.

Separate common cations in as little as four minutes using methanesulfonic acid (MSA) or sulfuric acid eluents with Thermo Scientific™ Dionex™ IonPac™ CS12A Cation-Exchange Columns. These medium-capacity, carboxylate-functionalized cation-exchange columns are recommended for fast, isocratic separation of lithium, sodium, ammonium, potassium, magnesium, and calcium in diverse sample matrices. The IonPac CS12A column is the recommended replacement for all IonPac CS12 column applications.<h5>Analysis of Common Cations plus Ammonium in Diverse Sample Matrices</h5><ul><li>Superior fast isocratic separation of mono- and divalent cations plus ammonium in diverse, complex matrices</li><li>Isocratic separation of Group I and II cations plus ammonium in 15 minutes in diverse matrices using a 250mm column</li><li>Fast, high resolution, isocratic separation of Group I and II cations plus ammonium in 4 minutes in simple matrices using 150mm column</li><li>Higher-capacity applications use 250mm columns, allowing injection of more concentrated samples without overloading or peak broadening</li><li>High efficiency, fast analysis use the 150mm column for increased sample throughput</li><li>Dionex IonPac CS12A-MS column provides fast elution at the low flow rates required for IC-MS, making it the ideal choice for IC-MS screening</li><li>Compatible with organic solvents, excluding alcohols, to enhance analyte solubility, modify column selectivity or allow effective column cleanup</li></ul>The Dionex IonPac CS12A-8μm and CS12A-5μm Capillary Columns offer the same selectivity as their analytical-scale counterparts with lowering operating costs.<h5>Recommended for Common Cations and Ammonium in Diverse Sample Matrices</h5><ul><li>Source water and drinking water, municipal and industrial wastewater, soil extracts, acid digests</li><li>Industrial cooling water, scrubber solutions; plating baths; and industrial solvents</li><li>Power plant waters treated with ammonium or morpholine; chemical additives; chemical process solutions</li></ul><h5>Reduce Operating Costs With Lower Eluent Requirements</h5>The Dionex IonPac CS12A capillary columns provide the same performance as the standard bore and microbore Dionex IonPac CS12A columns because they are packed with the same material. Operating costs are reduced because the capillary system operates at only 1% of the eluent flow rate of the standard bore system, requiring less eluent and producing less waste.<h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4x250mm, 2x250mm, 3x150mm or 2x100mm analytical columns</li><li>4x50mm or 2x50mm, 3x30mm guard columns</li><li>0.4x250mm, 0.4x150mm capillary columns</li><li>0.4x50mm or 0.4x35mm capillary guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Shorter 150mm and 100mm columns produce faster runs of relatively clean matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4>Specifications</h4><ul><li>Crosslinking: 55% DVB</li><li>Column Capacity: 2800 μeq</li><li>For Use With (Application): Cation Exchange</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: Acidic Eluents, 0 to 100% Acetonitrile, Alcohols Should be Avoided</li><li>Stationary phase: IonPac CS12A-8μm</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac CS14 IC Columns

Thermo Scientific Dionex IonPac CS14 IC is moderately low capacity cation-exchange column for analysis of aliphatic amines, aromatic amines, and polyamines using MSA or sulfuric acid eluents.

Obtain consistent reliable results for the analysis of aliphatic amines, aromatic amines, and polyamines using methanesulfonic acid (MSA) or sulfuric acid eluents with the Thermo Scientific™ Dionex™ IonPac™ CS14 Cation-Exchange Column. The column facilitates resolution of a large number of aliphatic amines and Group I and Group II cations in diverse sample matrices. However, the newer Thermo Scientific™ Dionex™ IonPac™ CS17, CS19 and CS19-4μm cation-exchange columns are recommended for these separations.Sample matrices can include environmental waters; power plant waters treated with ammonium, morpholine or ethanolamine; chemical additives; chemical process solutions; scrubber solutions; plating baths; industrial solvents; and wastewater.<h5>Determination of Amines in Diverse Matrices</h5><ul><li>Medium low-capacity carboxylate-functionalized hydrophilic cation-exchange column optimized for determination of a variety of amines</li><li>Dionex IonPac CS19 and CS17 columns are recommended replacement columns for most Dionex IonPac CS14 column applications</li><li>Dionex IonPac CS19 and CS19-4μm columns are recommended for polar amines, including alkanolamines and methylamines; and moderately hydrophobic and polyvalent amines, including biogenic amines and alkyldiamines</li><li>Compatible with organic solvents, (THF up to 20%), to enhance analyte solubility, modify column selectivity, or allow effective column cleanup</li></ul><h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4x250mm, 2x250mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul><h4>Specifications</h4><ul><li>Crosslinking: 55% DVB</li><li>Column Capacity: 1300 μeq</li><li>For Use With (Application): Cation Exchange</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: Acidic Eluents, 0 to 100% HPLC Solvents, Except TFH 0 to 20% Compatible, Alcohols Should be Avoided</li><li>Stationary phase: IonPac CS14</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Products focused on LC columns from Thermo Fisher Scientific - page 2

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