Size exclusion chromatography (SEC) columns are highly sensitive to operating conditions, including the composition of the mobile phase and the nature of analyzed samples. Improper handling and unsuitable method conditions can significantly reduce column lifetime. This guide summarizes common causes of column degradation and provides practical recommendations for extending column lifespan.<h3>Common Causes of Reduced Column Lifetime</h3>Several factors can negatively affect the performance and durability of SEC columns:<h4>1. Contamination of the Stationary Phase</h4><ul><li>Accumulation of impurities from samples or mobile phases</li><li>Microbial growth due to infrequent replacement of mobile phase</li></ul><h4>2. Stationary Phase Degradation</h4><ul><li>Caused by unsuitable pH conditions</li><li>Exposure to inappropriate temperatures</li><li>Use of harsh chemical agents</li></ul><h4>3. Improper Column Storage</h4><ul><li>Incorrect storage conditions may reduce column performance over time</li></ul><h4>4. Column Inlet Blockage</h4><ul><li>Particulates from insufficiently prepared samples can clog the column inlet</li></ul><h3>Preventive Measures and Best Practices</h3><h4>1. Preventing Contamination</h4><ul><li>Use high-quality solvents</li><li>Prepare buffer solutions daily</li><li>Filter both mobile phases and samples before use</li><li>Perform regular column cleaning</li></ul>Important note: Not all SEC columns can be reverse flushed. For example:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534">Biozen dSEC-2</a> and Yarra SEC-X7 columns must not be backflushed</li></ul><h4>2. Protecting the Stationary Phase</h4><ul><li>Operate within recommended pH and temperature limits</li><li>When using strong denaturants (e.g., urea or SDS):<ul><li>Perform immediate and thorough water flushing after use</li></ul></li></ul><h4>3. Proper Column Storage</h4>Before storage always clean the column thoroughly<ul><li>Two storage options:<ul><li>Buffer with bacteriostatic agent (e.g., sodium azide)</li><li>No buffer: water with 20% methanol</li></ul></li></ul>When introducing methanol:<ul><li>Use low flow rates</li><li>Prevent pressure spikes that could damage the column</li></ul>When restarting the column:<ul><li>Consider that the column contains 20% methanol</li><li>Adjust flow conditions accordingly</li></ul><h4>4. Preventing Column Inlet Clogging</h4>Use protective components:<ul><li>Guard column</li><li>Precolumn</li></ul>These act as barriers to prevent contamination of the analytical column.<h5>Types of Protection:</h5><ul><li>Security guard cartridges<ul><li>Minimal added length</li></ul></li><li>Short precolumns<ul><li>Same stationary phase as analytical column</li></ul></li></ul><h5>Key Advantage:</h5><ul><li>Essential for columns that cannot be backflushed</li><li>Protects against:<ul><li>Poorly centrifuged samples</li><li>Particulate contamination</li><li>Dirty sample matrices</li></ul></li></ul><h3>Conclusion</h3>Extending the lifetime of SEC columns requires careful attention to:<ul><li>Sample preparation</li><li>Mobile phase quality</li><li>Operating conditions</li><li>Storage procedures</li><li>Use of protective accessories</li></ul>Implementing these practices helps prevent contamination, degradation, and mechanical damage, ensuring consistent column performance and longer service life.

Extending the Lifetime of Size Exclusion Columns

<h4>Let’s begin with a simple question: what do we actually mean by high throughput?</h4>In the laboratory, high throughput usually refers to the ability to analyze tens, hundreds, or even thousands of samples per day, either across a laboratory workflow or on a single analytical system. Achieving this requires more than just a fast method. It depends on reliable instrumentation, automation, sufficient sample capacity, and smart system design that minimizes idle time between runs.In this article, we look at the key requirements for high-throughput HPLC analysis and how they are addressed in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2371">HTQC system</a>.<h4>Speed</h4>High-throughput HPLC workflows typically rely on short methods. This includes both the chromatographic runtime and the cycle time of the injection module.With the HTQC system, fast gradient applications up to 1200 bar can be implemented using two integrated UHPLC high-pressure gradient pumps.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_2_HPG_pumps_of_HTQC_system_left_P_8_1_L_max_pressure_1240_bar_right_P_6_1_L_max_pressure_1000_bar_4d4873ff2f.jpg" alt="KNAUER: Fig. 1–2 HPG pumps of HTQC system, left: P 8.1L (max. pressure 1240 bar), right: P 6.1L (max. pressure 1000 bar)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_2_HPG_pumps_of_HTQC_system_left_P_8_1_L_max_pressure_1240_bar_right_P_6_1_L_max_pressure_1000_bar_4d4873ff2f.jpg 245w," sizes="100vw" width="1266" height="386">We will not discuss the full details of transferring classical HPLC methods to fast, high-resolution UHPLC methods here, but UHPLC implementation can dramatically reduce total analysis time.Let’s use a hypothetical example. Imagine Giorgia, a randomly chosen name, working in pharmaceutical quality control in 1998. She must have travelled back in time, because she would have been too young to work in a lab then, but the example still works. With a classical HPLC method, each sample takes approximately 75 minutes, including equilibration, analysis, and backflushing of a 250 × 4.6 mm ID column. This type of method has been common in QC laboratories around the world. During an eight-hour working day, Giorgia can analyze around six samples.Now let’s transfer the same method to UHPLC. The improvement is substantial. Analysis time, equilibration time, and backflushing time are all reduced to under six minutes per sample. Giorgia can now process around 90 samples in one working day.You may wonder why the HTQC system requires two UHPLC gradient pumps. We will come back to that shortly.The next way to increase speed is to reduce injector cycle time. Injection procedures and washing steps can sometimes take a significant amount of time. This is where the LH 8.1 liquid handler becomes important.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_3_KNAUER_LH_8_1_Liquid_Handler_77ab506ca6.webp" alt="KNAUER: Fig. 3 KNAUER LH 8.1 Liquid Handler" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_3_KNAUER_LH_8_1_Liquid_Handler_77ab506ca6.webp 129w," sizes="100vw" width="800" height="971">The LH 8.1 supports overlapped injections. This means that the next injection can already be prepared while the current analysis is still running. Necessary cleaning steps of the injection system are still maintained, but idle time is reduced.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Schematic_buildup_of_injections_with_and_without_overlapped_injections_af089ba652.webp" alt="KNAUER: Fig. 4 Schematic buildup of injections with and without overlapped injections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Schematic_buildup_of_injections_with_and_without_overlapped_injections_af089ba652.webp 245w," sizes="100vw" width="1920" height="452">Let’s return to Giorgia. By using overlapped injections in her QC method, she saves about one minute per sample. This increases her capacity to approximately 105 samples per day.<h4>Sample Throughput</h4>A high-throughput system must also be able to handle and store a sufficient number of samples. Here, the LH 8.1 provides another major advantage. In the long-rail version, it can be equipped with up to three robotic coolers.With three coolers, each containing three drawers, the system can hold nine 10 × 13 sample trays. That means a total capacity of 1170 vials. The samples are also temperature controlled.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_5_6_Robotic_Cooler_with_two_10x6_trays_LH_8_1_887_version_with_two_Robotic_Coolers_855b10c2a3.jpg" alt="KNAUER: Fig. 5–6 Robotic Cooler with two 10x6 trays, LH 8.1 (887 version) with two Robotic Coolers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_5_6_Robotic_Cooler_with_two_10x6_trays_LH_8_1_887_version_with_two_Robotic_Coolers_855b10c2a3.jpg 245w," sizes="100vw" width="1262" height="503">Combined with the low eluent consumption typical of UHPLC, this large sample capacity makes it possible to extend operation from an eight-hour workday to 24-hour operation.For Giorgia and her QC lab, this changes everything again. The same UHPLC method, including overlapped injections, can now run continuously around the clock. This allows approximately 320 samples to be processed per day.Is that enough? Maybe not. As the blog title suggests: we just can’t get enough.<h4>Automation</h4>In addition to a standard 6-port, 2-position injection valve, the HTQC system includes an additional valve. This special column-switching and backflushing valve enables two columns to be operated alternately within a single UHPLC system.While one column is used for analysis, the second column can be backflushed and equilibrated in the reverse flow direction at the same time. This is particularly useful for reducing matrix effects, which are often encountered in QC laboratories when complex sample matrices are analyzed. It also helps reduce overall method runtime.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_7_Exemplary_flow_scheme_of_special_column_switching_valve_d837a71117.webp" alt="KNAUER: Fig. 7 Exemplary flow scheme of special column switching valve" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_7_Exemplary_flow_scheme_of_special_column_switching_valve_d837a71117.webp 127w," sizes="100vw" width="735" height="905"><h4>Parallel Processing</h4>Remember the earlier question: why does the system use two UHPLC gradient pumps?The reason is now clear. The second gradient pump is required to backflush one column while the other column is connected to the analytical flow path through the special switching valve.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_8_Schematic_illustration_of_column_cleaning_equilibration_and_analysis_with_special_backflush_valve_eae2e6f705.webp" alt="KNAUER: Fig. 8 Schematic illustration of column cleaning, equilibration and analysis with special backflush valve" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_8_Schematic_illustration_of_column_cleaning_equilibration_and_analysis_with_special_backflush_valve_eae2e6f705.webp 245w," sizes="100vw" width="1920" height="281">Giorgia immediately ordered a second UHPLC pump and the special valve for her QC lab. She now has a complete HTQC system and is very pleased with the result.Why? Let’s calculate it one final time. With all automation features in place, Giorgia can clean one column while the other column analyzes a sample. This enables her to process an impressive 600 samples per day.Compared with the classical HPLC method she used in 1998, the daily sample throughput has increased from six samples to 600 samples. That is a 100-fold increase.<h4>Summary</h4>We started with a question, so let’s end with one too: what steps increased the throughput?First, the method was scaled down from classical HPLC to a fast UHPLC approach. Then, a second pump and a special column-switching and backflushing valve were added to the UHPLC setup. Together with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1454">LH 8.1 liquid handler</a> and its exceptional sample capacity, the HTQC system becomes a flexible and powerful configuration for high-throughput analysis.The system can also be coupled to nearly any detector used in HPLC. Because of its ability to operate at very high speed, it is especially well suited for mass spectrometric methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_9_Complete_flow_scheme_of_HTQC_system_with_highlighted_system_components_edef36cb42.webp" alt="KNAUER: Fig. 9 Complete flow scheme of HTQC system with highlighted system components" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_9_Complete_flow_scheme_of_HTQC_system_with_highlighted_system_components_edef36cb42.webp 161w," sizes="100vw" width="800" height="775"><blockquote>For further information on this topic, please contact our authors: kramer@knauer.net, m.myrach@knauer.net</blockquote>

Introducing Our System Solution for High Throughput Quality Control – HTQC

News from LabRulezLCMS Library - Week 19, 2026

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Thermo Scientific Hypersil GOLD C18 Selectivity HPLC Columns

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. 

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical data.<h5>Novel Bonding</h5><ul><li>Proprietary derivatization and endcapping</li><li>C18 (USP L1) selectivity</li></ul><h5>pH Stability</h5><ul><li>Robust performance at high pH</li><li>Excellent column stability at low pH</li></ul><h5>Flexible Formats</h5><ul><li>Javelin HTS columns for high-throughput screening</li><li>Semi-preparative columns with 12 μm particles available</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<ul><li>Excellent peak symmetry</li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: LC/MS</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C18 Selectivity</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon Load: 11%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 1 to 11</li></ul>

Thermo Scientific Accucore Biphenyl Reversed Phase HPLC Columns

Choose Thermo Scientific™ Accucore™ Biphenyl LC Columns for fast and reliable separation of critical pairs and isomers.

Choose Thermo Scientific™ Accucore™ Biphenyl LC Columns for fast and reliable separation of critical pairs and isomers. These columns provide unique selectivity for aromatic and moderately polar analytes. Their optimized packing offers a rugged platform for a variety of matrices. Based on Core Enhanced Technology, Accucore liquid chromatography reversed phase columns provide fast, high-resolution separations without the elevated backpressures seen using sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-mode selectivity for aromatic and moderately polar analytes</li><li>Enhanced Pi-pi interactions with aromatics</li><li>Excellent lot-to-lot reproducibility</li></ul>Trust your separation of critical pairs and isomers to Accucore Biphenyl LC Columns. These columns offer fast run times, excellent robustness, and consistent reproducibility, and provide the perfect balance of polar analyte retention and hydrophobic analyte elution.<h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC/UHPLC</li><li>Max. Pressure: 8700 psi (600 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Biphenyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 6%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Thermo Scientific Accucore Vanquish C18+ UHPLC Columns

Choose Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC columns, containing uniquely efficient 1.5 μm solid core particles for use with the Thermo Scientific™ Vanquish™ UHPLC system

Choose Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC columns, containing uniquely efficient 1.5 μm solid core particles for use with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=40&amp;manufacturerItems=3">Thermo Scientific™ Vanquish™ UHPLC system</a>. Founded on state-of-the-art Core Enhanced Technology™, this sub-2 μm particle next-generation column has ultra-short diffusion paths that result in extremely efficient separations. This high efficiency enables fast separation and the resolution of complex mixtures. In concert with Vanquish systems, these columns deliver more results with better separations, thereby enhancing workflows and productivity.<h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: UHPLC</li><li>Max. Pressure: 21756 psi (1500 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: C18</li><li>Surface Area: 110 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L1</li><li>Carbon Load: 6.5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 9</li></ul>

Thermo Scientific Accucore RP-MS HPLC Columns

Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.

Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations—without the elevated backpressures required by sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Optimized for MS detection</li><li>Excellent peak shapes</li><li>Excellent combination of speed and efficiency</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Proprietary</li><li>Surface Area: 130 m²/g</li><li>Temperature: 60°C</li><li>USP Type: N/A</li><li>Carbon Load: 7%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 9</li></ul>

Thermo Scientific Accucore C18 HPLC Columns

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

Achieve fast, high-resolution separations at low backpressures using Thermo Scientific™ Accucore™ C18 LC columns. Rugged 2.6μm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms.&nbsp;The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Optimal Selectivity for Nonpolar Compounds</h5><ul><li>Hydrophobic interaction mechanism</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: C18</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L1</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 1 to 11</li></ul>

Thermo Scientific Accucore C8 HPLC Columns

Improve separation speed and resolution while maintaining low backpressures using Thermo Scientific™ Accucore™ C8 LC Columns. 

Improve separation speed and resolution while maintaining low backpressures using Thermo Scientific™ Accucore™ C8 LC Columns. Rugged 2.6μm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms.&nbsp;The high coverage C8 bonded phase provides optimal retention of analytes with moderate hydrophobicity. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Lower Hydrophobic Retention</h5><ul><li>Recommended for moderately hydrophobic analytes or for when a less hydrophobic phase provides optimum retention</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: C8</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L7</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 9</li></ul>

Thermo Scientific Accucore aQ C18 Polar Endcapped HPLC Columns

Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns.

Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.The polar endcapped C18 stationary phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Alternative Selectivity</h5><ul><li>Polar endcapped C18 stationary phase provides optimum retention of polar analytes</li><li>Accucore aQ Columns are compatible with highly aqueous mobile phases.</li><li>Polar encapping allows use in 100% aqueous mobile phases without loss of performance or stability.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Polar</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: C18</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L1</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 9</li></ul>

Thermo Scientific Accucore Polar Premium HPLC Columns

Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns.

Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.&nbsp;The robust amide-embedded C18 stationary phase provides unique selectivity complementary to conventional C18 columns, is stable from pH 1.5–10, and is fully compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Alternative Selectivity</h5><ul><li>Polar embedded reverse phase material offers complementary selectivity to standard C18 columns.</li></ul><h5>Stable over Wide pH Range</h5><ul><li>Specially designed bonded phase is stable from pH 1.5–10.5.</li></ul><h5>Compatible with Highly Aqueous Mobile Phases</h5><ul><li>Rugged amide-embedded C18 phase will not undergo phase collapse in 100% aqueous mobile phase.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Polar Embedded</li><li>Surface Area: 90 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L60</li><li>Carbon Load: 9%</li><li>Phase: Reversed Phase</li><li>Pore Size: 150 Å</li><li>pH: 1.5 to 10.5</li></ul>

Thermo Scientific Accucore Phenyl-Hexyl HPLC Columns

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes</li><li>Enhanced Pi-pi Interactions with Aromatics</li><li>Moderate Hydrophobicity</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Phenyl/Hexyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer confidently and routinely characterize, quantify, and confirm in a single analysis using a single mass spectrometer.

Take Quanfirmation™ to the next level. The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer introduced the revolutionary concept of Quanfirmation. The Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer takes Quanfirmation to the next level with increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies.<h5>Superior Quantitation</h5><ul><li>Advanced quadrupole technology (AQT) improves precursor selection and transmission for more-accurate quantitation of low-abundance analytes in complex matrices</li><li>Sophisticated data-independent acquisition (DIA) and parallel reaction monitoring (PRM) deliver reproducible quantitation with complete qualitative confidence</li><li>An advanced active beam guide (AABG) reduces noise and extends maintenance intervals</li></ul><h5>Enhanced Analysis of Intact Proteins</h5><ul><li>Optional intact protein mode provides superior trapping of large molecules for improved analysis of intact proteins and protein complexes</li><li>Optional enhanced resolution ensures maximum ID confidence in top-down proteomics and lipidomics</li></ul><h5>High-Confidence Results</h5><ul><li>HR/AM and full-scan capabilities capture all sample data, all of the time, enabling retrospective data analysis without need to re-run samples</li><li>Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices</li><li>Better than 1-ppm mass accuracy in full- and all-ion fragmentation (AIF) scan modes ensures confident compound identification</li><li>Fast scanning at 12 Hz and spectral multiplexing suited to UHPLC applications</li><li>Rapid polarity switching maximizes information obtained</li><li>AIF and multiple dissociation techniques — in-source CID and HCD — aid in compound identification</li><li>Mass range to 6000 m/z enhances detection of singly charged small molecules and biomolecules</li><li>More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan</li></ul>

Products from Thermo Fisher Scientific - page 15

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