Microplastics and Nanomaterials: Understanding Emerging Contaminants in Environmental Testing

<p><strong>🎤Matthew Bolino</strong></p><p><strong>How do the hidden carbohydrate structures on your favorite protein powders shape the gut microbiome? &nbsp;In this episode of Concentrating on Chromatography, Matthew Bolino, M.S., from the University of Nevada, Reno, breaks down his latest research on N‑glycans from common dietary proteins (whey, egg white, soy, and pea) and how their structural diversity influences microbial fermentation and short‑chain fatty acid production.</strong></p><p>Bolino explains what N‑glycans are, why they behave like fiber in the gut, and how his team isolates and characterizes them using ethanol washes, enzymatic release (PNGase F and gut‑derived endoglycosidases), and advanced MALDI‑TOF and HILIC‑QTOF workflows. &nbsp;He also discusses his 2025 work comparing synthetic versus bovine whey N‑glycomes and mapping N‑glycan profiles across dietary protein sources, revealing how glycan architecture can reshape community diversity in in vitro fecal fermentations.</p><p>Geared toward undergraduate and early‑career analytical chemists, this conversation dives into practical mass spec trade‑offs (<strong>MALDI vs QTOF vs LC/GC</strong>), real‑world troubleshooting in glycomics labs, and how microbiome‑targeted therapeutics and “symbiotic” designs may emerge from pairing specific microbes with preferred glycan structures. &nbsp;Bolino closes with career advice on building biomolecular analysis skills, understanding instrumentation fundamentals, and entering the rapidly growing field of glycomics and microbiome research.</p><h3><strong>Video Transcription</strong></h3><h4><span><strong>Introduction</strong></span></h4><p>Glycomics has emerged as a rapidly developing field within biomolecular science, expanding our understanding of post-translational modifications beyond well-known processes such as phosphorylation. Among these modifications, <strong>protein glycosylation</strong>—the covalent attachment of carbohydrate structures to proteins—plays a critical role in biological function, molecular recognition, and cellular communication.</p><p>This discussion focuses on <strong>N-glycans</strong>, a class of structurally complex carbohydrates linked to proteins, and their occurrence in common dietary protein sources such as whey, egg white, soy, and pea protein. Particular emphasis is placed on their structural diversity, analytical workflows, and emerging relevance in gut microbiome research.</p><h4><span><strong>Fundamentals of N-Glycans</strong></span></h4><p>N-glycans are oligosaccharide structures covalently attached to proteins via nitrogen atoms, typically at asparagine residues. They represent one of the two major classes of protein glycosylation, alongside O-glycans.</p><p>These glycans are characterized by:</p><ul><li><strong>High structural diversity</strong>, arising from variations in monosaccharide composition and linkage patterns</li><li><strong>Large size</strong>, typically comprising 7–15 monosaccharide units</li><li><strong>Complex branching architectures</strong>, leading to a vast number of possible configurations</li></ul><p>Common monosaccharide components include:</p><ul><li>Mannose</li><li>Galactose</li><li>N-acetylglucosamine</li><li>N-acetylgalactosamine</li><li>Sialic acids</li></ul><p>Unlike free carbohydrates present in foods (e.g., glucose, lactose), N-glycans are <strong>protein-bound</strong>, which significantly influences their biological behavior.</p><h4><span><strong>Biological Function and Dietary Relevance</strong></span></h4><p>N-glycans play multiple roles in biological systems, including:</p><ul><li>Modulation of <strong>protein stability</strong></li><li>Regulation of <strong>protein trafficking and localization</strong></li><li>Facilitation of <strong>cell–cell communication and immune recognition</strong></li></ul><p>They are particularly abundant on <strong>membrane and surface proteins</strong>, where they contribute to molecular signaling processes.</p><p>From a dietary perspective, N-glycans exhibit unique behavior. Due to their size and protein-bound nature, they are not readily digested in the upper gastrointestinal tract. Instead, they can reach the colon, where they may function analogously to <strong>dietary fiber</strong>, interacting with the gut microbiota.</p><h4><span><strong>Research Motivation</strong></span></h4><p>The investigation of N-glycans in dietary proteins was initially driven by interest in protein nutrition and supplementation, particularly in the context of muscle growth and protein quality. However, this focus expanded upon recognition of the importance of glycosylation in biological systems.</p><p>The field of glycomics offers:</p><ul><li>Extensive <strong>structural variability</strong>, enabling diverse biological functions</li><li>Relevance in <strong>disease mechanisms</strong>, including cancer, where glycosylation patterns can influence immune evasion</li><li>Opportunities to explore <strong>diet–microbiome interactions</strong></li></ul><p>This combination of nutritional, biochemical, and biomedical relevance makes N-glycan research particularly compelling.</p><h4><span><strong>Experimental Workflow for N-Glycan Analysis</strong></span></h4><p>The analysis of N-glycans requires a multi-step workflow due to their covalent attachment to proteins.</p><h5><span><strong>1. Protein Purification</strong></span></h5><p>Dietary protein samples often contain:</p><ul><li>Free sugars</li><li>Lipids</li><li>Other matrix components</li></ul><p>To isolate glycoproteins:</p><ul><li><strong>Cold ethanol precipitation</strong> is used to remove free carbohydrates</li><li>Multiple washing steps ensure removal of residual sugars</li><li>A <strong>phenol–sulfuric acid assay</strong> is applied to confirm the absence of free sugars</li></ul><h5><span><strong>2. Enzymatic Release of N-Glycans</strong></span></h5><p>N-glycans are enzymatically cleaved from proteins using:</p><ul><li><strong>PNGase F (Peptide-N-Glycosidase F)</strong></li></ul><p>This enzyme:</p><ul><li>Specifically cleaves the glycan from the protein backbone</li><li>Preserves the intact glycan structure</li></ul><p>Alternative approaches using <strong>gut-derived enzymes</strong> are being explored to improve accessibility and yield.</p><h5><span><strong>3. Glycan Purification and Labeling</strong></span></h5><p>Following release:</p><ul><li>Glycans are purified from the protein matrix</li><li>Chemical labeling is performed (e.g., 2-AA or procainamide tagging)</li></ul><p>Labeling enhances:</p><ul><li>Detection sensitivity</li><li>Chromatographic behavior</li></ul><h5><span><strong>4. Analytical Techniques</strong></span></h5><p>Two primary analytical platforms are employed:</p><ul><li><strong>MALDI-TOF MS</strong><ul><li>High sensitivity</li><li>Broad structural coverage</li></ul></li><li><strong>HILIC-LC-MS</strong><ul><li>Improved reproducibility</li><li>Enhanced chromatographic separation</li></ul></li></ul><p>These approaches provide <strong>semi-quantitative data</strong>, where signal intensity reflects relative abundance rather than absolute concentration.</p><h4><span><strong>Analytical Challenges</strong></span></h4><p>Several challenges are associated with N-glycan analysis:</p><h5><span><strong>Low Abundance</strong></span></h5><ul><li>Dietary proteins typically contain only <strong>1–2% glycan content by weight</strong></li><li>This limits yield and complicates downstream applications</li></ul><h5><span><strong>Sample Preparation Complexity</strong></span></h5><ul><li>Extensive purification is required to eliminate free sugars</li><li>Contamination risks must be carefully managed</li></ul><h5><span><strong>Instrumentation Trade-Offs</strong></span></h5><ul><li>Different analytical platforms offer complementary advantages</li><li>No single technique provides complete structural and quantitative information</li></ul><h4><span><strong>Differences Between Plant and Animal N-Glycans</strong></span></h4><p>Comparative analysis reveals distinct differences in glycan composition:</p><h5><span><strong>Plant-Derived Proteins (e.g., soy, pea)</strong></span></h5><ul><li>Predominantly <strong>high-mannose structures</strong></li><li>Lower monosaccharide diversity</li><li>Simpler glycan architectures</li></ul><h5><span><strong>Animal-Derived Proteins (e.g., whey, egg white)</strong></span></h5><ul><li>Greater <strong>structural complexity</strong></li><li>Inclusion of diverse monosaccharides (e.g., sialic acids)</li><li>More elaborate branching patterns</li></ul><p>These differences have important implications for biological function and microbial interactions.</p><h4><span><strong>Implications for Gut Microbiome Research</strong></span></h4><p>N-glycans represent a potential class of <strong>prebiotic substrates</strong> that can influence gut microbiota composition and activity.</p><p>Key findings suggest:</p><ul><li>Structural diversity of glycans affects <strong>microbial utilization patterns</strong></li><li>More complex glycans may promote <strong>greater microbiome diversity</strong></li><li>Specific glycan structures may selectively stimulate beneficial microbes</li></ul><p>This has led to growing interest in:</p><ul><li><strong>Synbiotic strategies</strong>, combining microbes with preferred substrates</li><li>Tailored dietary interventions to enhance <strong>metabolic outputs</strong>, such as short-chain fatty acids</li></ul><h4><span><strong>Methodological Considerations</strong></span></h4><p>A critical insight from this work is the importance of understanding <strong>analytical trade-offs</strong>:</p><ul><li>MALDI-TOF MS provides broader detection of glycan structures</li><li>LC-MS approaches offer greater reproducibility</li></ul><p>No single technique is universally optimal; instead, method selection should be guided by the specific research question.</p><h4><span><strong>Conclusion</strong></span></h4><p>N-glycans in dietary proteins represent a complex and biologically significant class of biomolecules with implications spanning nutrition, microbiology, and disease research. Advances in analytical methodologies continue to improve our ability to characterize these structures, despite challenges related to low abundance and structural diversity.</p><p>A deeper understanding of N-glycan composition and function may enable the development of targeted nutritional strategies and microbiome-based therapeutics, highlighting the growing importance of glycomics in modern life sciences.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

N‑Glycans, Whey Protein, and the Gut Microbiome: Mass Spec Insights with Matthew Bolino

<div><div><div><p><strong>Our immune system is incredibly powerful: among other things, it can recognize and destroy cancer cells. But tumors can be just as effective at avoiding detection – they build “invisibility shields” to protect themselves from attack.</strong></p></div></div><div><p>One breakthrough in cancer therapy has been immune checkpoint inhibitors (ICIs), drugs that reactivate immune T cells and help them fight tumors again. However, only about 20–50% of patients respond well to this treatment. Researchers are therefore seeking alternative therapeutic strategies. A small and well-known molecule called adenosine plays a key role in these efforts.</p><p>When tumor cells are stressed or dying, they release ATP, which is normally a signal that something is wrong in the tissue. But in the tumor microenvironment, ATP gets rapidly broken down by two enzymes, CD39 and CD73. Released adenosine acts like a chemical “calm-down” signal, binds to immune cells (T cells, NK cells, and others), and tells them to slow down, reducing their ability to kill cancer cells. Targeting CD73 therefore represents a promising strategy for developing new cancer treatments.</p></div><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg" alt="IOCB Prague: Development of potent inhibitors to boost the immune system's ability to fight cancer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg 245w," sizes="100vw" width="661" height="363"></p><p>At IOCB Prague, interdisciplinary teams led by Jan Řezáč, Jan Konvalinka, and Michal Hocek used a newly developed high-throughput screening method to test over 5,000 compounds, identifying two ribonucleoside derivatives as promising CD73 inhibitors.</p><p>The scientists then improved the compounds’ activity by carefully modifying the ribonucleoside scaffold with bulky (het)aryl groups. Further optimization improved the pharmacokinetic properties of these compounds, yielding inhibitors with low clearance, long half-life, high solubility, and strong selectivity over related enzymes such as CD39.</p><p>In cellular experiments, the compounds reduced adenosine production in cancer cells, restored T cell activation, and showed no toxicity toward human fibroblasts. The most active compound showed a single-digit picomolar inhibition constant (Ki).</p><p>These findings establish a strong foundation for further in vivo efficacy and combination studies, which will explore the therapeutic potential of this novel inhibitor class.</p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707"><strong>Potent Competitive Inhibitors of Ecto-5′-nucleotidase (CD73) based on 6-(Het)aryl-7-deazapurine Ribonucleoside 5′-O-Bisphosphonates</strong></a></h4><p>Ugnė Šinkevičiu̅tė, Magdalena Šímová, Radek Staník, Lenka Poštová Slavětínská, Kristýna Blažková, Pavel Šácha, Martin Lepšík, Jan Řezáč, Jan Konvalinka, Tereza Ormsby, Michal Tichý, Michal Hocek</p><p><i><span><strong>ACS Pharmacol. Transl. Sci.</strong></span></i><strong> </strong><span><strong>2026, 9, 1, 191–213</strong></span></p><p><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">https://pubs.acs.org/doi/10.1021/acsptsci.5c00707</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>CD73 generates immunosuppressive adenosine in the tumor microenvironment and is a promising target for cancer immunotherapy. We have designed and systematically studied diverse 2-substituted 7-deazapurine ribonucleoside 5′-<i>O</i>-bisphosphonates bearing a variety of (het)aryl groups at position 6 and discovered their highly potent and selective CD73 inhibition activity. The most active compounds (with single-digit picomolar <i>K</i><sub>i</sub>) contained bicyclic (het)aryl groups at position 6 in combination with chlorine at position 2. Further optimization of pharmacokinetic properties identified inhibitors with low clearance, long half-life, high solubility, and excellent selectivity over CD39 and NTPDase3. They effectively suppressed adenosine formation in MDA-MB-231 cells, rescued CD8<sup>+</sup> T cell activation, and were nontoxic to human fibroblasts. Overall, their profile compares favorably with <strong>AB680</strong>, a CD73 inhibitor currently in phase I/II clinical trials.</p></div>

Development of potent inhibitors to boost the immune system's ability to fight cancer

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Video

Scientists

Environment

Scientific article

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Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

New Food magazine

Agilent Technologies

Technology Networks

Phenomenex

Thermo Fisher Scientific

SelectScience

Waters Corporation

<h4><strong>HPLC Mobile Phases and Gradients</strong></h4><ul><li>Explore the impact of mobile phase selectivity on chromatographic resolution.</li><li>Take a deep dive into typical mobile phase compositions; pH, buffers, organic solvents and other additives</li><li>Understand the significance of pH, and how to effectively design gradients</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Anyone who wants to learn about basics of mobile phase selectivity in liquid chromatography (focusing on reverse phase LC) and it’s significance in method development.</li></ul><blockquote><p><strong>Presenter</strong>: Namrata Saxena (Technical Manager, Phenomenex)</p><p>Namrata is the Technical Manager for the Asia-Pacific region in Phenomenex and is based in Australia. As a member of the global technical team, she is responsible for assisting customers and the internal marketing and sales teams of Phenomenex for multifarious technical queries. She also support company’s bio-products portfolio through external and internal seminars/webinars, training, and technical content creation. Her competence and focus lie in providing chromatographic solutions for characterization workflows of biotherapeutics and new and emerging CGT modalities. Before joining Phenomenex she had worked as a Product Specialist and spent several years in stationary phase chemistry, column manufacturing and method development groups in a German company Bischoff Chromatography GmbH. Namrata has a post graduate degree with specialization in Analytical Chemistry.</p></blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Mobile Phases and Gradients

Take a deep dive into typical mobile phase compositions; pH, buffers, organic solvents and other additives.

<div><div><p><strong>Let's go back to the Basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.</strong></p><p>This journey started in 2024 and we are now pleased to invite you to join us to go deeper in fundamentals, troubleshooting and application specific topics.</p><h3><strong>EPISODE 9: How to Analyze RNA &amp; Oligonucleotides</strong></h3><p><strong>What will you learn?</strong></p><ul><li>End-to-end Sequence Mapping and CQA Monitoring Workflows</li><li>LNP Characterization and Monitoring</li></ul><p><strong>What's next?</strong></p><ul><li>Check out the full Webinar Series Episodes List <a target="_blank" rel="noopener noreferrer" href="https://event.on24.com/eventRegistration/EventLobbyServlet?target=reg20.jsp&amp;eventid=4817889&amp;sessionid=1&amp;key=B9182AE813B3F8A30217606156F96B75&amp;groupId=5901784&amp;sourcepage=register"><strong>here</strong></a></li></ul><p><strong>Not sure to be able to attend live sessions?&nbsp;</strong></p><ul><li>Register today and watch the webinars on-demand</li></ul><blockquote><p><strong>Presenter: </strong>Antonia Wierzbicki (Principal MS Market Dev Manager, EMEA, Waters Corporation)&nbsp;</p><p>Antonia Wierzbicki is a MS market development manager at Waters working in EMEA. Antonia has BSc in Pharmaceutical Science and a MSc in Cancer Pharmacology. Following several years working as an analytical chemist in drug development roles within both academic labs and contract research organizations, Antonia has more recently spent time working with analytical chemists to provide solutions using cutting edge technologies to meet their needs and requirements, with a specific interest in ultra-sensitive quantitation of small molecules. Antonia is based in Cambridge UK and keen to connectwith like-minded scientists in the field.</p><p><strong>Presenter:&nbsp;</strong>Andrea Gheduzzi (Principal LC Market Dev. Manager, EMEA, Waters Corporation)</p><p>Andrea Gheduzzi, is a principal LC market development manager for EMEA at Waters Corporation. Andrea Gheduzzi has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab manager position, and technical manager for chromatography columns. He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his previous role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications. Since 2023 he became principal LC market development manager for EMEA and member of the AQbD working party at the EDQM.</p><p><strong>Presenter:&nbsp;</strong>Ben Wilkes (Principal Biologics Market Development Manager, Waters Corporation)</p><p>Since 2022, Ben Wilkes is a Principal Biologics Market Development Manager at Waters where he connects biotech companies with enabling analytical technologies, focusing on liquid chromatography and mass spectrometry. Prior to Waters, Ben worked at Lonza Biologics from 2015 to 2022 as an analytical R&amp;D scientist, supporting all aspects of a products lifecycle with novel analytical methods. He also previously worked at Abzena and Sanofi-Genzyme after graduating from the University of Manchester in 2012</p></blockquote></div></div>

Waters Corporation: Back to Basics Advanced Webinar Series 2025

Back to Basics Advanced - Biologics: RNA & Oligonucleotides

End-to-end Sequence Mapping and CQA Monitoring Workflows. LNP Characterization and Monitoring.

<p><strong>Join Waters experts as they explore EU regulations on PFAS in food, analytical challenges across complex matrices, and case studies with renowned food testing labs.</strong></p><p>baby chewing cucumber resizedPer- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants that accumulate in food and pose growing concerns for regulators, laboratories, and consumers. In response, the European Commission has introduced stringent recommendations and regulations, including (EU) 2022/1431 and (EU) 2023/915, which define limits and monitoring requirements for PFAS in food, particularly animal-origin products and infant foods.</p><p>Analysing PFAS in food is scientifically demanding. Complex matrices, the diversity of PFAS structures, and strict LOQ requirements create significant analytical challenges. From fruits and vegetables to fish and baby food, laboratories face issues such as matrix effects, contamination risks, and separation of branched versus linear isomers. Eggs, in particular, represent a challenging case study due to their dense protein and fat content, yet are increasingly tested for PFAS exposure linked to environmental and dietary factors.</p><p>This webinar will provide essential regulatory context, discuss critical analytical obstacles, and share practical insights into achieving accurate, robust results across diverse food types. Case studies will highlight sensitivity requirements, method performance, and analysis results, illustrating the complexities of PFAS testing and the innovations helping laboratories succeed.</p><p>Attendees will gain both a broad perspective and actionable strategies to meet compliance requirements and advance food safety testing.</p><h4><strong>Key learning points</strong></h4><ul><li>Understand EU regulatory requirements and recommendations for PFAS monitoring in food.</li><li>Explore analytical challenges including matrix effects, LOQs, and isomer separation.</li><li>Learn from case studies on PFAS detection in fish, fruits, and baby food.</li><li>Gain insights into method performance, calibration, and quality assurance guidance.</li><li>Discover how environmental and dietary factors influence PFAS exposure in food.</li><li>Engage with experts and get practical answers to PFAS testing questions.</li></ul><blockquote><p><strong>Presenter:</strong> Claudia Rathmann (Principal F&amp;E Market Development Manager – Waters Corporation)</p><p>Experienced and passionate analytical science professional with over 20 years of technical and strategic expertise in food and environmental applications. Currently serving as Principal Market Development Manager at Waters Corporation, Claudia specialiszes in connecting customers with product management to deliver innovative solutions for complex analytical challenges. With a strong scientific foundation and a proven track record in both technical and marketing roles, Claudia bridges the gap between customer needs and product innovation across the EMEA region.</p></blockquote>

New Food Magazine: PFAS in Food: Regulations, Analytical Challenges, and Case Studies

PFAS in Food: Regulations, Analytical Challenges, and Case Studies

Join Waters experts as they explore EU regulations on PFAS in food, analytical challenges across complex matrices, and case studies with food testing labs.

PFAS in Food: Regulations, Analytical Challenges, Studies

<p><strong>Accurate measurement of protein as well as excipient concentration is essential in downstream biopharmaceutical manufacturing and process control, spanning from post-purification stages to final preparation for fill and finish.</strong></p><p>Join Dr. Janam Pandya and Patrick Brown as they explore how UV-Vis and Raman spectroscopy work together to deliver a complete picture of bioprocess performance. Learn how rapid, at-line UV-Vis analysis provides precise quantification of highly concentrated protein samples, while complementary, in-line Raman spectroscopy continuously monitors protein concentration and excipients in real time.</p><p>By integrating these two optical techniques, scientists can connect fast at-line confirmation with continuous in-line insight, enabling data-driven decisions that enhance process understanding, control, and product quality.</p><h4><strong>Key learning objectives:</strong></h4><ul><li>Understand how UV-Vis and Raman spectroscopy complement one another across at-line and in-line workflows.</li><li>Learn how a new at-line UV-Vis technology enables accurate measurement of highly concentrated protein samples.</li><li>Discover the strengths of process Raman spectroscopy in quantifying protein concentration and excipients in complex matrices.</li><li>Explore how process Raman spectroscopy can be applied for in-line analysis to support process decision-making.</li></ul><h4><strong>Who should attend?</strong></h4><ul><li>Scientists developing standard operating procedures (SOPs) and improving manufacturing processes, including upstream/downstream, MSAT, and PAT roles.</li><li>R&amp;D and technical experts exploring new technologies including lab managers, QA/QC, professors, engineers, and purchasing agents.</li></ul><h4><strong>Certificate of attendance</strong></h4><ul><li>If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</li><li>If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Janam Pandya (Product Manager, Measurement Control and Sample Identification, Thermo Fisher Scientific)</p><p>Dr. Janam Pandya is a scientist and spectroscopist with 10 years of academic and industry experience. Dr. Pandya obtained his Ph.D. in food science from the University of Massachusetts Amherst; there his research focused on developing novel analytical methods utilizing cutting-edge Raman spectroscopic techniques. Since 2022, he has worked for Thermo Fisher Scientific as an application scientist, advancing the application of Raman Spectroscopy for real-time process measurements. This includes his current focus on implementing process Raman spectroscopy as a PAT tool in the clean-energy sector, among other industries.</p><p><strong>Presenter:</strong> Patrick Brown (Senior Product Manger, Thermo Fisher Scientific)</p><p>Patrick Brown graduated with a masters in Biomedical Science from the Pennsylvania State University and an MBA from University of Delaware. He now leads the UV-Vis Product Management team for Thermo Fisher Scientific, where he works with his team to collect user feedback, and then integrate this feedback into software and hardware updates.</p><p><strong>Moderator: </strong>Olivia Long (Editorial Team, SelectScience)</p></blockquote>

SelectScience: Connecting at-line UV-Vis with in-line process Raman for real-time protein and excipient monitoring

Connecting at-line UV-Vis with in-line process Raman for real-time protein and excipient monitoring

Join us to explore how UV-Vis and Raman spectroscopy work together to deliver a complete picture of bioprocess performance. 

Connecting at-line UV-Vis with in-line process Raman

<p><strong>Join us for an exciting seminar dedicated to the latest innovations and techniques in the biopharma industry. This event will showcase cutting-edge workflows in proteomics and structural biology, highlighting how these advancements are transforming biopharmaceutical research and development.</strong></p><h4><strong>Why Attend?</strong></h4><ul><li><strong>Explore customer workflows:</strong> Discover how leading companies are implementing innovative workflows to drive efficiency and effectiveness in their research and development processes.</li><li><strong>Discover new product innovations: </strong>Learn about the latest products that are revolutionizing the biopharmaceutical industry.</li><li><strong>Network with industry leaders:</strong> Meet with experts and peers to gain valuable insights and stay at the forefront of biopharmaceutical innovations.</li></ul><h4><strong>Topics Covered:</strong></h4><ul><li><strong>Proteomics:</strong> Dive into the latest techniques and advancements in proteomics.</li><li><strong>Structural Biology: </strong>Explore the cutting-edge developments in structural biology.</li><li>and more</li></ul><h4><strong>Preliminary Agenda:</strong></h4><h5><strong>10:00 Welcome coffee registration</strong></h5><h5><strong>Single cell and immunopeptidomics explorations on Orbitrap-Astral&nbsp;</strong></h5><ul><li>Farida Salimova and Jeewan Babu Rijal, LSMBO</li></ul><h5><strong>Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow&nbsp;</strong></h5><ul><li>Jeff Op de Beeck, R&amp;D Staff Scientist, Thermo Fisher Scientific</li></ul><h5><strong>Host Cell Protein on the Thermo Scientific Orbitrap Astral&nbsp;</strong></h5><ul><li>Severine Clavier, Sanofi</li></ul><h5><strong>12:00 Lunch break</strong></h5><h5><strong>13:30 Driving the process development of recombinant therapeutic products with automated mass spectrometry-based target peptide mapping&nbsp;</strong></h5><ul><li>Diego Bertaccini, Merck</li></ul><h5><strong>15:00 Coffee break &amp; networking opportunities (End of the event around 15:30)</strong></h5><h4><strong>Who Should Attend?</strong></h4><ul><li>Biopharmaceutical researchers and scientists</li><li>Industry professionals and leaders</li><li>Anyone interested in the latest biopharma innovations</li></ul><p>Participation is free of charge. <strong>A registration is required.&nbsp;</strong><br>The event language is English.</p><p>Don't miss out on this opportunity to enhance your knowledge and network with industry leaders. Register now to secure your space!</p><p>For more information about the event or for questions regarding your registration, please contact <strong>analyze.eu@thermofisher.com</strong>.</p><p>We look forward to seeing you at the seminar!</p><blockquote><p><strong>Presenter:</strong> Information about speakers will be published soon</p></blockquote>

Thermo Fisher Scientific: Omics innovations for the biopharmaceutical industry

Omics innovations for the biopharmaceutical industry

Join us for an exciting seminar dedicated to the latest innovations and techniques in the biopharma industry.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Learn how integration and automation can be used to improve consistency, data integrity, scalability in this case study focusing on how an analytical development lab achieved this using connected software.</strong></p><h5><strong>In this webinar, you will learn:</strong></h5><ul><li>How to identify and address common pain points in disconnected lab environments (data silos, manual transfers, and inconsistent reporting)</li><li>How connected software integrates instrument control, data acquisition, and analysis into one centralized workflow</li><li>Practical approaches for ensuring data integrity, regulatory approvals, and advancing toward the connected “lab of the future” (including advanced technologies from Thermo Fisher Scientific)</li></ul><p>In this educational session, Da Ren of BioTherapeutics Solutions LLC, will share a case study on deploying a fully integrated software environment in the lab.</p><p>You’ll see how connected software solutions streamling workflows (in this case, analytical development) by centralizing instrument monitoring, data acquisition, and analysis. This helps teams reduce costs, improve scalability, and maintain compliance.</p><p>Analytical development in biologics and pharmaceuticals often struggles with familiar bottlenecks: maintaining data integrity, meeting regulatory requirements, scaling processes, and maintaining consistency across teams. Traditional lab setups, where instruments and data systems operate in isolation, make these challenges even harder to overcome.</p><p>We’ll also discuss how integrated platforms align with emerging expectations for the “lab of the future”, from supporting regulatory approvals to improving training and reproducibility using analytical chemistry applications as examples.</p><p>Join us to learn practical strategies you can apply in your own workflows, and see how connected labs are shaping the future of biologics development.</p><blockquote><p><strong>Presenter: </strong>Da Ren (Founder and CEO, BioTherapeutics Solutions LLC)</p><p>Dr. Da Ren, founder of BioTherapeutics Solutions (BTS), is a trailblazer in the field of Quality Control with his development of the Multi-Attribute Method (MAM). This innovative approach has not only optimized efficiency but also ensured product quality, earning acceptance from over 30 regulatory agencies worldwide. Beyond his pioneering work, Dr. Ren actively contributes to the industry by serving on advisory boards and participating in organizations such as the MAM Consortium. He holds a Ph.D. in Analytical Chemistry from the University of Akron and has held influential roles at both Amgen and Waters Corporation.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Integrated Software Solution for Analytical Development in the Biopharmaceutical Industry

Integrated Software Solution for Analytical Development in the Biopharmaceutical Industry

Discover how integrated software streamlines analytical development, enhances data integrity, and supports the connected lab of the future through a case study.

Software Solution for Biopharmaceutical Industry

<div><p><strong>High-throughput LC-MS/MS workflows can dramatically boost efficiency in routine contaminant analysis, but understanding the technical limits of mass spectrometry remains essential to achieving consistent, high-quality results.</strong></p><p>This webinar explores how different LC-MS/MS systems were evaluated and how new technological advancements enhanced data quality and precision at trace levels.</p><p>Attendees will gain valuable insight into optimizing workflows for the rapid detection of over 900 mycotoxins, plant toxins and secondary metabolites – learning practical strategies for enhancing data reliability and operational sustainability across analytical laboratories.&nbsp;</p><h4><strong>Attend this webinar to:</strong></h4><ul><li>Explore cutting-edge advancements in LC-MS/MS technology that are driving superior data quality and precision</li><li>Learn how ultra-fast MRM acquisition rates on the SCIEX 7500+ system enables detection of over 900 routine contaminants in under 11 minutes – without compromising data quality</li><li>Hear how increasing throughput enhanced efficiency can cut up to 22 tons of CO2 and deliver financial benefits to analytical labs</li></ul><blockquote><p><strong>Presenter:</strong> Lidija Kenjeric (Research Associate, Austrian Competence Centre for Feed, Food, Quality, Safety and Innovation, FFoQSI)</p><p>Lidija Kenjeric is a PhD candidate at the Austrian Competence Centre for Feed, Food, Quality, Safety and Innovation and her research focuses on developing high-throughput LC–MS/MS methods to enhance the efficiency of contaminant monitoring in food and environmental systems.</p></blockquote></div>

Technology Networks: Enhancing Efficiency in LC-MS/MS Analysis for Rapid Contaminant Detection

Enhancing Efficiency in LC-MS/MS Analysis for Rapid Contaminant Detection

Learn how high-throughput LC-MS/MS boosts precision, detects 900+ contaminants fast, improves sustainability through next-gen MRM workflows.

LC-MS/MS for Rapid Contaminant Detection

<p><strong>Mass spectrometry (MS)-based proteomic biomarker discovery pipeline commonly begins with large-scale profiling techniques detecting thousands of candidate biomarkers. Efficiently triaging these biomarker candidates for advancement to the biomarker verification stage of the biomarker development pipeline is not trivial. Historically, immunoassays have been used as the method of choice for targeted protein quantification. However, these antibody-based methods include several limitations, including cross-reactivity due to poor specificity and selectivity, which limits the multiplexing abilities of these assays.</strong></p><p>Targeted MS methods such as parallel reaction monitoring (PRM) can overcome these limitations by identifying and quantifying peptides and proteins in complex biological samples with high accuracy, sensitivity, and reproducibility. A recent advancement in PRM is its application on a novel mass spectrometry platform, the Thermo Scientific™ Stellar™ mass spectrometer which features a dual-pressure linear ion trap mass analyzer. The Stellar MS has significantly enhanced PRM capabilities facilitating quantitative targeted MS analyses that require high measurement precision and accuracy, low limits of detection/quantification, ruggedness, and throughput. In this webinar, Dr. Thomas will provide a glimpse into how PRM on the Stellar MS can be used as a high-throughput targeted MS approach to identify and prioritize novel predictive proteomic biomarkers of drug resistance in ovarian cancer.</p><h5><strong>Learning Objectives:</strong></h5><ul><li>Learn how mass spectrometry can be applied during the verification stage of biomarker development</li><li>Understand the enhanced PRM capabilities of the Stellar MS in supporting targeted mass spectrometry applications</li><li>Learn key concepts from consensus guidelines regarding the use of targeted MS for peptide and protein quantification</li></ul><blockquote><p><strong>Presenter:</strong> Stefani Thomas, PhD, University of Minnesota, Assistant Professor</p><p>Stefani earned her BA in Biological Sciences with a Minor in Music from Dartmouth College and her PhD in Pharmaceutical Sciences from the University of Southern California. After completing a research postdoctoral fellowship in the lab of Dr. Robert Cotter at Johns Hopkins, Stefani joined the Center for Biomarker Discovery and Translation at Johns Hopkins where she was a member of the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC). She then completed a Clinical Chemistry postdoctoral fellowship at Johns Hopkins and earned board certification from the National Registry of Certified Chemists and the American Board of Clinical Chemistry. Currently, Stefani is an Assistant Professor in the Department of Laboratory Medicine and Pathology at the University of Minnesota, she is the Associate Medical Director of the M Health Fairview West Bank Laboratory, and she has authored more than 70 publications. Her research laboratory applies mass spectrometry-based clinical proteomics to elucidate the biology of ovarian cancer towards improved patient stratification for therapeutic treatment.&nbsp;</p></blockquote>

Thermo Fisher Scientific: Breaking Barriers: Practical Approaches to Translating Proteins from Biomarker Candidates to Verified Biomarkers

Breaking Barriers: Practical Approaches to Translating Proteins from Biomarker Candidates to Verified Biomarkers

Learn how Stellar MS enhances PRM for targeted proteomics, enabling precise biomarker verification and helping identify predictive markers of drug resistance.

Approaches to Translating Proteins from Biomarker Candidates

<div><p><strong>Mycotoxins and plant toxins continue to pose a hazard in our food supply. &nbsp;With environmental factors and changing consumer preferences influencing the risk of exposure, the need for reliable, yet scalable analytical methods is paramount to enable continued monitoring.</strong></p><p>Join us for an interactive session, as we delve into LC-MS/MS based analytical methodologies developed for the determination of high impact ergot alkaloids in relevant foodstuffs to meet the needs of the food industry. Artificially contaminated samples of rye, oat, wheat, wheat gluten and baby food were extracted comparing three different solvents (acidic, neutral and alkaline) and extraction procedures with different amounts of d-SPE sorbent, to find the best combination of high extraction efficiency and effective clean-up results. Don’t miss your chance to ask our panelists your questions.</p><h4><strong>Register today to explore:</strong></h4><ul><li>Global factors and changing dynamics that may impact the risk and occurrence of contamination from mycotoxins and associated plant toxins.</li><li>Method development and analytical challenges, to meet Commission Regulations (EU) 2023/915 &nbsp;- applicable from 1st July 2024 - to ensure brand protection and consumer safety.</li><li>Considering the quantification of 8 out of 12 regulated compounds by EU (sum of –ine and –inine forms), this approach can be defined as a screening method for a reliable estimation of the risk.</li></ul><blockquote><p><strong>Presenter:</strong> Eleonora ROLLO (PhD candidate – Food Safety Researcher, Barilla SpA | University of Parma)</p><p>I’m a sporty girl with a deep love for good food and good wine. My curiosity has always pushed me to ask simple but essential questions: What am I eating? How is it made? Is it safe for me? These questions guided my academic path, from Food Science and Technology (Bachelor’s Degree) to Food Safety (Master’s Degree), and finally into a PhD in Barilla Group. Becoming a Food Safety Researcher was a natural choice: it allows me to combine my personal passion for food with the scientific mission of protecting consumers’ health.</p><p>Scientific production documented by 1 special issues journals, 7 contributions at national and international conferences and 2 scientific papers.</p><p><strong>Presenter: </strong>Michele Suman (Food Safety &amp; Authenticity Senior Scientist Research Manager | Adjunct Professor of AgriFood Authenticity, Barilla SpA)</p><p>Food Safety &amp; Authenticity Research specialist with over two decades of experience leading innovative research and development within the agri-food sector, with a track record of method development for studying contaminants, adulterants, authenticity markers and their transformation along food processing, pioneering new technologies as well. Expertise in coordinating complex, international funded projects, and a dedication to mentoring the next generation of professionals. Fostering collaboration and advancing the field through innovation with private/public stakeholders.</p><ul><li>Chair of the Italian National Normative Organization (UNI) Food Authenticity Working Group</li><li>Member of Joint FAO/WHO Expert Committee on Food Additives (JECFA)</li><li>Working Groups Toxins, Contaminants, Food Authenticity EU Committee for Standardization (CEN)</li><li>Member of ILSI Europe Scientific Advisory Committee &amp; Vice Chair of Food Contaminants Task Force</li><li>Coordinator of Quality-Food Safety Pillar - Italian National Cluster Agrifood Scientific Board</li><li>Co-Chair of Recent Advances in Food Analysis (RAFA) Conference series</li></ul><p>Scientific production documented by 6 book chapters, 3 special issues journals &amp; 3 books edited, 195 contributions at national and international conferences and 135 papers in international ISI journals.</p></blockquote></div>

Waters Corporation: Monitoring mycotoxins and detecting ergot alkaloids in cereals: Insights from food industry

Monitoring mycotoxins and detecting ergot alkaloids in cereals: Insights from food industry

Explore LC-MS/MS workflows for ergot alkaloid detection, extraction optimization, and meeting EU 2023/915 requirements to enhance food safety monitoring.

Monitoring mycotoxins & detecting ergot alkaloids in cereals

<p><strong>Join us as we celebrate The Agilent Infinity III LC Series turning one. Welcome to the Agilent Technologies Infinity III LC Series Webinar - The Infinity III Transition - Easing your usual regulatory pain points.</strong></p><h4><strong>Key learning objectives:</strong></h4><ul><li>Learn what's new from Agilent Infinity III LC systems with it's SMART features.&nbsp;</li><li>Understand the ease of upgrading your LC systems within a regulatory laboratory and when connecting to your current CDS system.&nbsp;</li><li>Allow us to share our special promotion to update your Infinity II systems – marking the one-year anniversary of the Agilent Infinity III launch.</li></ul><h4><strong>Who should attend?</strong></h4><p>Laboratory managers, lab instrument purchasing decision makers, lab analysts and end users of chromatography systems, and quality departments. &nbsp;</p><blockquote><p><strong>Presenter: </strong>Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)</p><p>Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</p></blockquote>

Agilent Technologies: Seamless Compliance – Effortless Transition from Infinity II to Infinity III LC Series

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