<div><p><strong>Mulching films used in agriculture gradually break down and can become a significant source of microplastics in soils. A new study shows that these particles affect not only soil structure but also microbial life and nitrogen cycling.</strong></p><p><i>“We wanted to understand how microplastics from both conventional and biodegradable mulching films influence soil properties, microbial diversity and functions in real conditions,”</i> explains <strong>Klára Šmídová</strong>, the study’s co-author . <i>“That’s why we added realistic amounts of microplastics to soils in experimental plots in Finland, Germany, and Spain, monitored them over two growing seasons, and compared them with the control plots.”</i></p><p>The results revealed that microplastics alter soil characteristics and microbial communities, although not always in the same way. <i>“ However, a consistent finding was the decline in microbial activity associated with key processes, including nitrogen cycling, with negative effects increasing at higher microplastic concentrations and becoming more pronounced in the second year,” </i>Šmídová adds. Because the microplastic levels used in the study reflect amounts already found in European soils, the findings highlight real risks to soil health. <i>“We also found that the effects were stronger in southern than in northern Europe, likely due to differences in climate and soil conditions. This means that protecting soils from plastic pollution will require region-specific risk assessments,”</i> she says.</p><p>Over 60% of European soils are estimated to be unhealthy, with ongoing degradation driven by unsustainable land use, contamination, climate change, and extreme weather events. In response, the EU Parliament adopted the Soil Monitoring Law in October 2025, aiming to achieve healthy soils across Europe by 2050. <i>“The law highlights the role of soil health in food security, water quality, and climate objectives, and establishes consistent soil monitoring across Member States. It includes measures to support farmers and foresters, while also creating a public list of potentially contaminated sites and an indicative watch list of emerging soil contaminants,” </i>explains Klára Šmídová.</p><p>The project brought together 19 research groups across Europe. <i>“After each season, hundreds of samples were colle cted and sent to partners for analysis. This paper focuses on soil properties, microbial diversity, and greenhouse gas emissions. Two more manuscripts—on plastic additives and microplastic fate, and on soil invertebrates—are in preparation. Together, they will give us a complete picture of how agricultural microplastics affect soil ecosystems,” </i>concludes Klára Šmídová.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg" alt="RECETOX: New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vysledky_nove_studie_ukazaly_ze_mikroplasty_meni_vlastnosti_pudy_a_mikrobialni_spolecenstva_i_v_realnych_podminkach_6ee3413a7b.jpg 171w," sizes="100vw" width="738" height="674"></p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0269749125015866?via%3Dihub#sec2"><span><strong>Conventional and biodegradable agricultural microplastics: effects on soil properties and microbial functions across a European pedoclimatic gradient</strong></span></a></h4><p>Šmídová K., Soinne H., Kim S. W., Tirronniemi J., Meffe R., Redondo-Hasselerharm P. E., Braun M., Rillig M. C., Fritze H., Adamczyk B., Nikama J., Kaseva J., Saartama V., Amelung W., Hurley R., Hofman J., Nizzetto L., Selonen S., Velmala S.</p><p>Environ. Pollut. 386 (2025) 127212</p><p><a href="DOI:%2010.1016/j.envpol.2025.127212"><span>DOI:&nbsp;10.1016/j.envpol.2025.127212</span></a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><div><p>Agricultural plastics like mulching films may become a major source of microplastic (MP) soil contamination during their degradation and fragmentation. This study investigates the effects of agricultural MPs from conventional (linear low-density polyethylene, PE) and biodegradable (starch-blended polybutylene adipate co-terephthalate, PBAT-BD) mulching films on soil physicochemical properties, aggregation, microbial diversity and functions, litter decomposition, and greenhouse gases emissions (GHG). For this purpose, MPs were mixed into soils at realistic MP concentrations of 0.005&nbsp;% and 0.05&nbsp;% (w/w) in 2022 on experimental plots in three EU countries representing different pedoclimatic conditions (Finland, Germany and Spain), followed by monitoring of the above-mentioned variables in the subsequent growing seasons 2022 and 2023. We found several significant MP-induced effects for soil properties, aggregation, microbial diversity, litter decomposition, and GHG, but the effect endpoints were less pronounced or varied considerably. Contrarily, microbial activity, contributing to soil functions such as nitrogen cycling, was consistently reduced by both conventional and biodegradable MPs. The reductions were more pronounced after the second season and for the higher MP treatment. As the higher MP concentration (i.e., 0.05&nbsp;% w/w) is environmentally relevant in Europe, our findings emphasize the potential effects of environmentally relevant MP concentrations on soil health. Furthermore, the effects increased from north to south, probably modulated by varying pedoclimatic conditions, inducing reflection of a need for regionally tailored risk</p></div></div>

New Study Reveals How Agricultural Plastics Impact Soil Health In Real Conditions

<h3><strong>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</strong></h3><p><strong>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711"></p><p>Among the analytical techniques available today, <strong>anion exchange chromatography (AEX)</strong> stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.</p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808"><strong>Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</strong></a></p></blockquote><h4><strong>How Scientists Read Oligonucleotides Using Charge</strong></h4><p>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.</p><p>As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.</p><h4><strong>Why Ion Exchange Works: Binding, Retention, and Release</strong></h4><p>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, <strong>anion exchange</strong> is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.</p><p>The system consists of two main components:</p><ul><li><strong>Stationary phase:</strong> A positively charged resin that binds anionic analytes.</li><li><strong>Mobile phase:</strong> An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul><p>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"></p><h4><strong>The Five-Step Workflow of Ion Exchange Chromatography</strong></h4><p>AEX separations generally follow a well-established sequence of steps:</p><ol><li><strong>Column equilibration</strong><br>The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li><strong>Sample loading</strong><br>Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li><strong>Washing</strong><br>The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li><strong>Elution</strong><br>Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li><strong>Regeneration and re-equilibration</strong><br>A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2  Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"></p><h4><strong>Why AEX Is Especially Effective for Oligonucleotides</strong></h4><p>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.</p><p>Key factors influencing AEX behavior include:</p><ul><li><strong>Charge density:</strong> Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li><strong>Resin chemistry:</strong> Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li><strong>Buffer composition:</strong> The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul><p>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"></p><h4><strong>AEX as the Gold Standard for Oligonucleotide Separation</strong></h4><p>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:</p><ul><li><strong>High resolving power:</strong> Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li><strong>Predictable retention behavior:</strong> In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li><strong>Compatibility with modified oligonucleotides:</strong> Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li><strong>Excellent control via gradient elution:</strong> Salt gradients provide precise tuning of selectivity and resolution.</li></ul><p>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"></p><h4><strong>Final Perspective: Mastering AEX with Confidence</strong></h4><p>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.</p><p>For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.</p><blockquote><p>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at <strong>sales@knauer.net</strong>. More insights into practical oligonucleotide analysis will follow in our <i>“Oligos Made Easy”</i> series.</p><p>For technical questions or in-depth discussion, the author can be reached at <strong>kindler@knauer.net</strong></p></blockquote>

Oligos Made Easy - Part 2: AEX

News from LabRulezLCMS Library - Week 07, 2026

Article

Application

Plastics

Environment

Science and research

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

SelectScience

Shimadzu Corporation

<p><strong>Are you tired of the endless entertainment options on Netflix and YouTube and looking for something with a bit more substance? Then you should join our educational webinar to learn what you can do to achieve the maximum column lifetime and performance from your LC columns.</strong></p>
<p><strong>Watch this webinar to learn:</strong></p>
<ul>
<li>What a typical lifetime for an HPLC column should be</li>
<li>How to identify the root cause for early column failure (based on chromatograms)</li>
<li>How to protect your column from solvent contaminations, particles and sample matrix</li>
<li>How to select the right column based on your method needs (e.g., temperature, pH, pressure range)</li>
<li>How to properly clean and store your HPLC column</li>
</ul>
<h3><strong>Who should attend:</strong></h3>
<ul>
<li>LC operators who are new to the HPLC world</li>
<li>Scientists keen to refresh their knowledge of LC column troubleshooting</li>
<li>Instrument self-maintainers who want to improve their skillset</li>
<li>Everyone interested in new products that help maximize column lifetime</li>
</ul>


SelectScience: Oh no! Not another boring webinar on column lifetime?

Oh no! Not another boring webinar on column lifetime?

<p><strong>This demonstration will concentrate on the enhanced workflows and capabilities of OpenLab CDS.</strong></p>
<h3><strong>During this 1-hour session you will:</strong></h3>
<ul>
<li>Learn about the function and advantages of the new control panel including creating instruments, creating projects, activity logs, storage types and paths.</li>
<li>Explore the basic operations of user privileges including assigning permissions and roles, and license utilities.</li>
<li>Learn the basics of how to setup instruments, methods and sequences.</li>
<li>Explore the enhanced data processing workflow including auto and manual integration, e-signatures and audit trail review</li>
<li>Discover uses for the Custom Calculator and its functionality</li>
<li>Learn how to customize reports with the Intelligent Reporter to generate calculations without to use of Excel</li>
</ul>
<blockquote>
<p><strong>Presenter: Richard Mutkoski (Application Engineer, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Richard Mutkoski began with Agilent (HP) in 1995. He has been an Application Engineer in Agilent’s Laboratory Informatics for the past 16 years. Prior to his career at Agilent Rich spent 12 years in a variety of small pharmaceutical firms in both R&amp;D and QA/QC capacities affording him first-hand knowledge of those workflows, bottlenecks and challenges.</p>
</blockquote>


Agilent Technologies: OpenLab CDS Introductory Demonstration

OpenLab CDS Introductory Demonstration

<p><strong>Even if you have robust quality systems governing your analytical laboratory and a strong data integrity program in place, you may still be nervous about preparing for a regulatory inspection. What will the inspectors want to know? What will they ask? Is your team prepared?</strong></p>
<p>You can ease those fears by attending this two-part webcast. First, we will explain what you should expect during an inspection. Then, we will simulate an inspection to show you, in a realistic scenario, what an inspector might ask, and how you and your team should handle the questions, including when they don’t know all the answers or don’t understand all the questions. We will explain the scope of a data integrity inspection in a regulated laboratory, and our simulation of an inspection of a chromatography data system (CDS) will explain the roles of different people in a multi-person team that includes a laboratory manager and the CDS laboratory administrator.</p>
<h3><strong>What we will cover:</strong></h3>
<ul>
<li>Background on data integrity and a chromatography data system (CDS)</li>
<li>Management involvement in data integrity</li>
<li>Qualification of chromatographs</li>
<li>Validation of analytical procedures</li>
</ul>
<h3><strong>Key Learning Objectives:</strong></h3>
<ul>
<li>Understand how data integrity plays a role in your inspection</li>
<li>Understand and answer the questions being asked</li>
<li>Understand the impact of poor analytical decisions</li>
<li>Use systems that can help demonstrate data integrity and regulatory compliance</li>
<li>Use technical controls to enforce operating procedures</li>
</ul>
<h3><strong>Who Should Attend:</strong></h3>
<ul>
<li>Analysts working in a regulated analytical laboratory</li>
<li>Chromatography data system (CDS) administrators</li>
<li>Laboratory managers</li>
<li>Quality assurance (QA) and quality control (QC) staff</li>
</ul>
<blockquote>
<p><strong>Presenter:  Bob McDowall (Director, RD McDowall Limited, Bromley, Kent, UK)</strong></p>
</blockquote>
<blockquote>
<p>Bob McDowall, PhD, is an analytical chemist with over 45 years of experience including 15 years working in the pharmaceutical industry and over 25 years working for the industry as a consultant. He is the director of R D McDowall Ltd, a consulting company offering services on process improvement, implementation of laboratory informatics applications, training staff in regulatory compliance, and qualification of IT infrastructure. Bob is a trained auditor and has been involved with the validation of computerized systems for over 30 years. He edited the first book on LIMS in 1987 and is the author of books on the validation of chromatography data systems and data integrity and data governance in regulated laboratories due to be published in late 2018. He was presented with the 1997 LIMS Award. He is a core industry expert for the GAMP Data Integrity Special Interest Group and was a subject matter expert contributor and reviewer of the 2017 GAMP Guide for Records and Data Integrity. He co-drafted the update to USP &lt;1058&gt; on Analytical Instrument Qualification (AIQ) for public comment that became effective in August 2017.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Paul Smith (Global Compliance Specialist, Agilent Technologies, Cheadle, UK)</strong></p>
</blockquote>
<blockquote>
<p>Paul is global compliance specialist with Agilent technologies. He has worked for Agilent for 9 years and has 35 years’ experience in the pharmaceutical and analytical area.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Josh Miller (OpenLab Software Marketing – eLearning Expert, Agilent Technologies, Santa Clara, CA)</strong></p>
</blockquote>
<blockquote>
<p>Josh is a professional chemist with 15 years’ experience in validating, supporting, deploying, and developing the OpenLab software suite of products. He is a true expert with all things OpenLab and has extensive experience with chromatography data system, electronic content management, business process management, instrument control products, and more. He has developed many of the training courses for OpenLab products and has served as an instructor for dozens of formal courses around the globe. In 2009, he created “eFamiliarization” to help customers learn the basics of OpenLab products in an interactive simulation of Agilent software. More recently he has transformed Agilent’s embedded software help systems to better serve customers by putting consistent and searchable content at their fingertips. You may also find a significant collection of video content that he produces to help users learn to use OpenLab products.</p>
</blockquote>


LCGC/Agilent Technologies: Five Essentials for Surviving Your Next Laboratory Inspection: A Quality Control Example, Part 1

Five Essentials for Surviving Your Next Laboratory Inspection: A Quality Control Example, Part 1

<p><strong>The Control Panel is the gatekeeper to the system.</strong></p>
<ul>
<li>Learn how to enable authentication to allow chemist names to be placed on reports. </li>
<li>See how to configure system in a compliant environment and restrict users’ access to instruments and data. </li>
<li>Understand the use of Roles and Privileges to refine what users can do. </li>
<li>Understand the need, creation and use of Projects. </li>
<li>Explore how to set-up an Acquisition Method for a Virtual 3D HPLC and a Virtual 7890 GC. </li>
<li>Explore the software features that have been designed to help minimize sequence creation time and eliminate costly typographical errors.</li>
</ul>


Agilent Technologies: OpenLab CDS Virtual Masterclass Series

OpenLab CDS Virtual Masterclass Series Session 1 - The Control Panel and Data Acquisition

<p><strong>Choosing the right column and conditions for your sample is a winning strategy.</strong></p>
<p>In this free webinar, we’ll look at the best matchups for your sample, including:</p>
<ul>
<li><p><strong>Column options: phase, particle size, dimensions</strong></p>
</li>
<li><p><strong>Conditions: pH, mobile phase</strong></p>
</li>
</ul>
<p>By the end, you’ll have additional tools to help you put together a game plan for a successful separation</p>
<blockquote>
<p><strong>Presenter:</strong> Mark Powell (Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.</p>
</blockquote>


Agilent Technologies: The LC Column Playbook

The LC Column Playbook

<h3><strong>This session will cover:</strong></h3>
<ul>
<li>What is chromatography and liquid chromatography?</li>
<li>What does an LC column do?</li>
<li>What types of samples can be analysed?</li>
<li>What makes up a Liquid Chromatography instrument?</li>
</ul>
<h3><strong>Future Sessions Topics will look to cover:</strong></h3>
<ul>
<li>Types of Chromatography</li>
<li>Common Issues, Tips and Tricks </li>
<li>Building your Method/Method Development</li>
<li>Optimising your Method &amp; Instrument</li>
<li>Troubleshooting</li>
</ul>


Shimadzu: LC Theory & Key Principles - Session 1: Introduction to Liquid Chromatography

LC Theory & Key Principles - Session 1: Introduction to Liquid Chromatography

<h3><strong>This session will cover:</strong></h3>
<ul>
<li>Most common types of liquid chromatography</li>
<li>Effects on compound separation</li>
</ul>
<h3><strong>Future Sessions Topics will look to cover:</strong></h3>
<ul>
<li>Common Issues, Tips and Tricks </li>
<li>Introduction to UHPLC</li>
<li>Building your Method/Method Development</li>
<li>Optimising your Method &amp; Instrument</li>
<li>Troubleshooting</li>
</ul>


Shimadzu: LC Theory & Key Principles - Session 2: Types of LC and Separation Techniques

LC Theory & Key Principles - Session 2: Types of LC and Separation Techniques

<p><strong>This session will cover:</strong></p>
<ul>
<li>The process of separation</li>
<li>How we use peak efficiency, retention, selectivity and resolution to measure the quality of a separation</li>
<li>What can we do to improve the quality of the separation</li>
</ul>
<h3><strong>Future Sessions Topics will look to cover:</strong></h3>
<ul>
<li>Common Issues, Tips and Tricks </li>
<li>Introduction to UHPLC</li>
<li>Building your Method/Method Development</li>
<li>Optimising your Method &amp; Instrument</li>
<li>Troubleshooting</li>
</ul>


Shimadzu: LC Theory & Key Principles - Session 3: The Separation Process, How it is Measured and Improved

LC Theory & Key Principles - Session 3: The Separation Process, How it is Measured and Improved

<p><strong>You will learn how to optimise your LC system to improve efficiency and get the most out advances in silica particles including superficially porous particles.</strong></p>
<h3><strong>This session will cover:</strong></h3>
<ul>
<li>Practically improve factors from the resolution equation</li>
<li>Optimise your LC system to improve efficiency</li>
<li>Get the most out of advances in silica particles including superficially porous particles</li>
</ul>
<h3><strong>Future Sessions Topics will look to cover:</strong></h3>
<ul>
<li>Common Issues, Tips and Tricks </li>
<li>Introduction to UHPLC</li>
<li>Building your Method/Method Development</li>
<li>Optimising your Method &amp; Instrument</li>
<li>Troubleshooting</li>
</ul>


Shimadzu: LC Theory & Key Principles - Session 4: Resolution Equation - Applying the Theory in Practice

LC Theory & Key Principles - Session 4: Resolution Equation - Applying the Theory in Practice

<p><strong>You will recap the key concepts from the previous sessions and ready ourselves for moving onto the advances in liquid chromatography series.</strong></p>
<h3><strong>This session will cover:</strong></h3>
<ul>
<li>Recap what we have learned in previous sessions</li>
<li>Ready ourselves for moving onto intermediate liquid chromatography</li>
</ul>
<h3><strong>Future session in other series will include:</strong></h3>
<ul>
<li>Common Issues, Tips and Tricks </li>
<li>Introduction to UHPLC</li>
<li>Building your Method/Method Development</li>
<li>Optimising your Method &amp; Instrument</li>
<li>Troubleshooting</li>
</ul>


LCMS webinars focusing on Academy/Principles - page 37