<div><div><div><p><strong>Our immune system is incredibly powerful: among other things, it can recognize and destroy cancer cells. But tumors can be just as effective at avoiding detection – they build “invisibility shields” to protect themselves from attack.</strong></p></div></div><div><p>One breakthrough in cancer therapy has been immune checkpoint inhibitors (ICIs), drugs that reactivate immune T cells and help them fight tumors again. However, only about 20–50% of patients respond well to this treatment. Researchers are therefore seeking alternative therapeutic strategies. A small and well-known molecule called adenosine plays a key role in these efforts.</p><p>When tumor cells are stressed or dying, they release ATP, which is normally a signal that something is wrong in the tissue. But in the tumor microenvironment, ATP gets rapidly broken down by two enzymes, CD39 and CD73. Released adenosine acts like a chemical “calm-down” signal, binds to immune cells (T cells, NK cells, and others), and tells them to slow down, reducing their ability to kill cancer cells. Targeting CD73 therefore represents a promising strategy for developing new cancer treatments.</p></div><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg" alt="IOCB Prague: Development of potent inhibitors to boost the immune system's ability to fight cancer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg 245w," sizes="100vw" width="661" height="363"></p><p>At IOCB Prague, interdisciplinary teams led by Jan Řezáč, Jan Konvalinka, and Michal Hocek used a newly developed high-throughput screening method to test over 5,000 compounds, identifying two ribonucleoside derivatives as promising CD73 inhibitors.</p><p>The scientists then improved the compounds’ activity by carefully modifying the ribonucleoside scaffold with bulky (het)aryl groups. Further optimization improved the pharmacokinetic properties of these compounds, yielding inhibitors with low clearance, long half-life, high solubility, and strong selectivity over related enzymes such as CD39.</p><p>In cellular experiments, the compounds reduced adenosine production in cancer cells, restored T cell activation, and showed no toxicity toward human fibroblasts. The most active compound showed a single-digit picomolar inhibition constant (Ki).</p><p>These findings establish a strong foundation for further in vivo efficacy and combination studies, which will explore the therapeutic potential of this novel inhibitor class.</p><blockquote><h4><strong>The Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707"><strong>Potent Competitive Inhibitors of Ecto-5′-nucleotidase (CD73) based on 6-(Het)aryl-7-deazapurine Ribonucleoside 5′-O-Bisphosphonates</strong></a></h4><p>Ugnė Šinkevičiu̅tė, Magdalena Šímová, Radek Staník, Lenka Poštová Slavětínská, Kristýna Blažková, Pavel Šácha, Martin Lepšík, Jan Řezáč, Jan Konvalinka, Tereza Ormsby, Michal Tichý, Michal Hocek</p><p><i><span><strong>ACS Pharmacol. Transl. Sci.</strong></span></i><strong> </strong><span><strong>2026, 9, 1, 191–213</strong></span></p><p><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">https://pubs.acs.org/doi/10.1021/acsptsci.5c00707</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>CD73 generates immunosuppressive adenosine in the tumor microenvironment and is a promising target for cancer immunotherapy. We have designed and systematically studied diverse 2-substituted 7-deazapurine ribonucleoside 5′-<i>O</i>-bisphosphonates bearing a variety of (het)aryl groups at position 6 and discovered their highly potent and selective CD73 inhibition activity. The most active compounds (with single-digit picomolar <i>K</i><sub>i</sub>) contained bicyclic (het)aryl groups at position 6 in combination with chlorine at position 2. Further optimization of pharmacokinetic properties identified inhibitors with low clearance, long half-life, high solubility, and excellent selectivity over CD39 and NTPDase3. They effectively suppressed adenosine formation in MDA-MB-231 cells, rescued CD8<sup>+</sup> T cell activation, and were nontoxic to human fibroblasts. Overall, their profile compares favorably with <strong>AB680</strong>, a CD73 inhibitor currently in phase I/II clinical trials.</p></div>

Development of potent inhibitors to boost the immune system's ability to fight cancer

<p><strong>In the week of April 13, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Agilent Technologies: The Journey of Safer Science through Safer Labs</strong></h5><ul><li>Tu, 14.4.2026 07:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6104">Registration</a></li></ul><p>Agilent Safer Labs webinar highlights best practices in lab safety, including handling, storage, and instrumentation, helping professionals build safer, more efficient laboratory environments.</p><h5><strong>2. Phenomenex: Strategies to Mitigate Sporadic PFAS Contamination in Analytical Workflows</strong></h5><ul><li>We, 15.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6095">Registration</a></li></ul><p>Discover how PFAS contamination enters LC-MS workflows and learn practical strategies to identify sources, prevent false positives, and ensure reliable PFAS analysis.</p><h5><strong>3. SCIEX: Tracking irrigation-related contaminants in water and soils (QTOF-MS)</strong></h5><ul><li>We, 15.4.2026 10:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6033">Registration</a></li></ul><p>Join this webinar to hear about benefits of ZenoTOF 7600 system for the analysis of pharmaceuticals and pesticides in wastewater, soils and crops.</p><h5><strong>4. Thermo Fisher Scientific: Imaging and top-down analysis of intact protein complexes in tissue</strong></h5><ul><li>Th, 16.4.2026 12:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6059">Registration</a></li></ul><p>Join this webinar to hear about latest methods and advances for probing intact protein complexes in tissue using in situ native mass spectrometry.</p><h5><strong>5. C&amp;EN: Precision Lab Solutions for High-End Optics and Coatings</strong></h5><ul><li>Th, 16.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6096">Registration</a></li></ul><p>Join this presentation and open discussion with industry-leading experts Patrick Muller and Kieran Evans – leaders in UV/Vis and IR analytical methods and testing techniques.</p><h5><strong>6. Agilent Technologies: Accelerating GLP-1 Analog Impurity Profiling with the Agilent Pro IQ Plus Followed by Detailed Sequence Analysis using Electron Capture Dissociation</strong></h5><ul><li>Th, 16.4.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5997">Registration</a></li></ul><p>Accelerate GLP-1 impurity profiling with single quad screening and LC/Q-TOF ExD analysis. Learn how ECD improves sequence confirmation and modification localization.</p><h5><strong>7. Agilent Technologies: Quantifying GLP-1 Peptides in Plasma: Bioanalytical Workflows for PK and Development</strong></h5><ul><li>Fr, 17.4.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5944">Registration</a></li></ul><p>Peptide 360 Episode 3 webinar on LC-MS bioanalysis of GLP-1 peptides in plasma for PK studies and development decisions.</p>

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<p><strong>This study introduces atmospheric solids analysis probe high-resolution tandem mass spectrometry with cyclic ion mobility as a novel approach for characterizing Asian lacquers. Applied to 27 historical samples from Japan, China, Vietnam, and Myanmar, the method enabled classification by geographic origin based on phenol and benzenediol derivatives.</strong></p><p>In addition to lacquer identification, the technique simultaneously detected pigments such as arsenic sulfides and associated resins, providing comprehensive chemical insight. This approach expands analytical capabilities for cultural heritage studies, supporting restoration, conservation, and art historical research.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572"><strong>Characterization of Asian lacquers by atmospheric solids analysis probe high resolution tandem mass spectrometry coupled with cyclic ion mobility separation</strong></a></h4><p>Vojtěch Zemek, Radek Ryšánek, Petra Krejčí, Lukáš Kučera, Jana Nádvorníková, Adéla Tůmová, Helena Heroldová, Adriana Stříbrná, Petr Bednář</p><p>Journal of Cultural Heritage, 77, 2026, 187-196</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.culher.2025.11.012">https://doi.org/10.1016/j.culher.2025.11.012</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>In recent years, an expansion of ambient pressure ionization mass spectrometry in various areas is observed [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0025"><span>25</span></a>]. Atmospheric solids analysis probe mass spectrometry (ASAP-MS) is a powerful technique for direct analysis of even very complex samples, such as polymers [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0026"><span>26</span></a>], foods [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0027"><span>27</span></a>], or petrochemical sources and products [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>]. ASAP ionization technique is a modification of atmospheric pressure chemical modification (APCI). In conventional arrangement, the sample is attached to a sampling probe tip (a standard melting point capillary is inserted into powder or liquid sample that sticks to its surface) and the probe is consequently placed into the ion source (commonly adapted ESI or APCI source). The analytes are released into the gas phase using hot gas (nitrogen) and ionized in the corona discharge. The formed ions (quasimolecular ions, radical cations or radical anions) are then transferred to the evacuated part of mass spectrometer [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0025"><span>25</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0028"><span>28</span></a>]. Note that especially at higher vaporization temperatures a portion of the analytes is pyrolyzed and a mixture of parent ions and pyrolysis product ions is commonly observed in the ASAP-MS spectra [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0029"><span>29</span></a>]. The main feature of this direct MS mode is a fast detection of the formed products (including those with a higher mass and perhaps a shorter lifetime). In contrast, due to chromatographic separation in py-GC/MS, only relatively stable and volatile products are detected (the chromatographic step provides an additional selectivity here). In addition, electron impact ionization produces significantly different spectra profiles compared to the atmospheric pressure chemical ionization ongoing in the ASAP source. Both techniques are to a certain extent complementary [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0024"><span>24</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0025"><span>25</span></a>,<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0030"><span>30</span></a>].</p><p>There is a direct possibility of coupling mass spectrometry with ion mobility spectrometry (IMS) to significantly improve the selectivity of analysis (and, to certain extent, to compensate the advantage of the chromatographic step in py-GC/MS while maintaining the direct MS analysis speed). During an ion mobility spectrometry experiment, ions of analytes are flying through the ion mobility cell (e.g., a drift tube or a closed-loop path within a traveling wave ion guide in case of the cyclic ion mobility cell) filled with buffer gas (e.g. helium). In this process, ions collide with the buffer gas. These collisions slow down the ions differently based on their size, shape and charge. Ion mobility spectrometry therefore allows separation of the present analytes in the gas phase according to their collision cross sections (CCS). It also allows separation of isomeric compounds and filtration of the matrix signals, resulting in a higher quality of the MS and MS/MS spectra [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0031"><span>[31]</span></a>, <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0032"><span>[32]</span></a>, <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0033"><span>[33]</span></a>].</p><p>ASAP-MS has a high potential for the study of protective and decorative layers on the surfaces of historical objects. To the best of our knowledge, however, ASAP-MS and ASAP-IMS-MS have never been used for characterization of lacquers. This paper deals with the development of ASAP-MS method for characterization of Asian lacquers and its application for classification of oriental historical objects.</p><h4><strong>3. Material and methods</strong></h4><h5><strong>3.3. Instrumentation and experimental setup</strong></h5><p>Analyses were performed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202"><strong>Waters Cyclic IMS high resolution tandem mass spectrometer</strong></a> with an integrated cyclic ion mobility cell and atmospheric solids analysis probe (ASAP) used as ion source (Waters, Milford, MA, USA). Samples (∼ 0.1–0.5 mg) were inserted into a melting point capillary (G119/32, manufacturer SAMCO, United Kingdom) through an in-lab radially drilled hole (hole diameter: approx. 1 mm; distance from the bottom end approximately 5 mm). Solution of 2′,4′,6′-trihydroxyacetophenone monohydrate (THAP) in acetone was used as a standard for lock mass correction (<i>c</i> = 1 mg.ml<sup>-1</sup>). The m/z values were calculated as the means from three repeated measurements of appropriate samples. Approx. 1 μl of standard was deposited onto the capillary using a small pipette tip. Following experimental parameters were used: desolvation temperature of 600 °C, source temperature of 100 °C, corona “current” mode (corona current 2.0 μA), cone voltage of 30 V, source offset of 10 V, cone gas flow of 30 l/min, desolvation gas flow of 500 l/min, nebulizer gas pressure of 6 bar and reference capillary voltage of 2.5 kV. As for the ion mobility separation setup, a cyclic ion mobility cell was used in the following sequence: 1. Inject (10 ms), 2. Separate (37 ms), 3. Inject to Pre-Store (0.50 ms), 4. Eject (0.50 ms), 5. Reinject from Pre-Store (0.63 ms), 6. Eject and Acquire (13.20 ms). TW static height was 11 V, TW ramp start height was 15 V and TW ramp end height was 35 V. Acquisition time was 3 min for the MS scan and 1 min for the MS/MS scan used for identification of analytes. In the MS/MS mode, collision energy 30 V was used for pentadecyl and heptadecylcatechols, 35 V for phenols substituted with (CH<sub>2</sub>)<sub>10</sub>C<sub>6</sub>H<sub>5</sub> and 40 V for catechols substituted with (CH<sub>2</sub>)<sub>12</sub>C<sub>6</sub>H<sub>5</sub> and phenols substituted with CH<sub>2</sub>CO(CH<sub>2</sub>)<sub>10</sub>C<sub>6</sub>H<sub>5</sub>, respectively. Scan time 1 s was used for both scans. At the end of each working day, sample V was measured for confirmation that the instrument measured consistently. Values of m/z were calculated by MassLynx software (version 4.2, SCN 1016) after lock mass correction using the signal of protonated molecule of THAP. Theoretical mass of particular analyte was used for lock mass correction in the MS/MS spectra. Centroid top at 80 % and areas of peaks were used for accurate mass and intensity of signals calculation. The maximal accepted difference from theoretical mass (dtm) of signals was 10 mDa. Peak areas were used to compare the abundance of identified analytes among particular samples. Mobilograms from cyclic ion mobility experiments were produced by DriftScope v2.9 software. In mobilograms, spectra were generated by integration at Full Width at Half Maximum (FWHM) of peaks at drift time 1.72 ms.</p><h4><strong>4. Results and discussion</strong></h4><h5><strong>4.1. Utilization of ASAP-MS</strong></h5><p>In the ASAP-MS spectra of the studied lacquers, signals of a several compounds were observed (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0002"><span>Fig. 2</span></a>). Signals of radical cations as well as protonated molecules of identified analytes were easily visible. Usually a slightly higher abundance of cation radicals compared to protonated molecules was observed (spectra of all samples are listed in Supplementary material B). The processes observed in the ASAP-MS spectra involve the release of alkyl phenol/alkyl catechol monomer(s) and cleavages of alkyl chain(s) after the carbon linked to the phenol/catechol ring (i.e. cleavage after the first, second and third alkyl carbons) and elimination of water either from polymer or released monomers (the pyrolytic/collision-induced fragmentation processes are discussed below in detail). The analysis of provided samples revealed the most important markers of specific types of lacquers.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Journal_of_Cultural_Heritage_77_2026_187_196_Fig_2_Spectra_of_lacquers_prepared_from_Toxicodendron_vernicifluum_Toxicodendron_succedaneum_and_Gluta_usitata_samples_U_V_and_B_0200c24c06.jpg" alt="Journal of Cultural Heritage, 77, 2026, 187-196: Fig. 2. Spectra of lacquers prepared from Toxicodendron vernicifluum, Toxicodendron succedaneum and Gluta usitata (samples U, V and B)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Journal_of_Cultural_Heritage_77_2026_187_196_Fig_2_Spectra_of_lacquers_prepared_from_Toxicodendron_vernicifluum_Toxicodendron_succedaneum_and_Gluta_usitata_samples_U_V_and_B_0200c24c06.jpg 245w," sizes="100vw" width="2750" height="1269"></p><h5><strong>4.1.4. Study of MS/MS spectra of characteristic markers</strong></h5><p>For all the standard materials, the most important markers were subjected to fragmentation after the collision induced dissociation (CID) in the Trap collision cell of the mass spectrometer (MS/MS experiments; all fragmentation spectra and observed diagnostic fragments including differences from theoretical masses are given in Supplementary material B).</p><p>Signals of fragments with an intensity higher than the specified percentage of well known fragments arising from studied compounds were investigated and identified. Diagnostic fragments are given in Table B.2, Supplementary material B. The threshold signal was set for different compounds. For heptadecyl- and pentadecylcatechols, the threshold signal was defined as 5 % of the signal of dihydroxytropylium that is always an integral component of their collision spectra. For heptadecenyl- and pentadecenylcatechols the threshold signal was 2.5 % of dihydroxytropylium. For pentadecatrienyl catechol it was 100 % of dihydroxytropylium while for dodecylphenylcatechol it was 1 % of dihydroxytropylium. In case of 1-(3-hydroxyphenyl)-12-phenyldodecan-2-one, the threshold signal was defined as 17 % of the signal of hydroxyphenylethan-2-one. Finally, for 10-decylphenylphenol and 12-dodecylphenylphenol, 50 % of the signal of methylphenol was used as the threshold signal. These fragments were used for confirmation of the presence of lacquer compounds in routine measurements (at least one MS/MS spectrum was measured in each sample). The threshold signals were suggested and set in order to reach a sufficient yield of diagnostic fragments for unambiguous identification of individual markers. These signals are intended to be used in long term measurement in practice.</p><div><p>Fragmentation of heptadecylcatechols with 0-3 double bonds (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0003"><span>Fig. 3</span></a>a-d) is similar to that of pentadecylcatechols with same number of double bonds. In all cases, the signals of the characteristic fragments are well separated from the signals of the matrix.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Journal_of_Cultural_Heritage_77_2026_187_196_Fig_3_Fragmentation_spectra_of_heptadecylcatechols_possessing_0_3_double_bonds_6149330bc3.jpg" alt="Journal of Cultural Heritage, 77, 2026, 187-196: Fig. 3. Fragmentation spectra of heptadecylcatechols possessing 0-3 double bonds, (MS/MS; m/z of parent ions are: a) 342.2559, b) 344.2715, c) 346.2872, d) 348.3028)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Journal_of_Cultural_Heritage_77_2026_187_196_Fig_3_Fragmentation_spectra_of_heptadecylcatechols_possessing_0_3_double_bonds_6149330bc3.jpg 232w," sizes="100vw" width="3000" height="2017"></p><h5><strong>4.2. Principal component analysis</strong></h5><p>Excellent separation of particular types of Asian lacquers in Score Plot (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0005"><span>Fig. 5</span></a>a-d) was obtained by Principal Component Analysis based on the benzenediol and phenol derivatives described above. The Score Plot contains a set of 20 samples obtained from the Náprstek Museum Prague as well as other samples (BBI, BBO, SBI, SBO, U, V and B) in which lacquer compounds were detected (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#tbl0002"><span>Table 2</span></a>). Note that in three other samples from The Náprstek Museum Prague, the lacquer compounds were not found at all.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Journal_of_Cultural_Heritage_77_2026_187_196_Fig_5_Score_plot_and_bi_plot_from_PCA_analysis_of_lacquers_taken_from_historic_objects_df82e0592a.jpg" alt="Journal of Cultural Heritage, 77, 2026, 187-196: Fig. 5. Score plot and bi-plot from PCA analysis of lacquers taken from historic objects, a. whole Score PCA plot, b. zoom of the T. succedaneum area, c. zoom of the T. vernicifluum area, d. zoom of the G. usitata area, e. whole PCA bi-plot, f. zoom of the T. succedaneum area in the PCA bi-plot, g. zoom of the T. vernicifluum area in the PCA bi-plot, h. zoom of the G. usitata area in the PCA bi-plot." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Journal_of_Cultural_Heritage_77_2026_187_196_Fig_5_Score_plot_and_bi_plot_from_PCA_analysis_of_lacquers_taken_from_historic_objects_df82e0592a.jpg 156w," sizes="100vw" width="3000" height="2994"></p><p>The significance of suggested markers for the studied lacquers across the whole data matrix was confirmed. The effect of particular markers is reflected in related bi-plots (<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#fig0005"><span>Fig. 5</span></a>e-h). Qualitatively, the results are in accordance with the former study obtained by py-GC/MS [<a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S1296207425002572#bib0001"><span>1</span></a>]. Since both cation-radicals and protonated molecules are formed in ASAP source with significant yield of both ion forms, their sum is an input into the data matrix for each substance (e.g., for pentadecenyl catechol, where the input is the sum of intensities I<sub>(m/z</sub> <sub>=</sub> <sub>318)</sub>+ <i>I</i><sub>(m/z</sub> <sub>=</sub> <sub>319)</sub>), see PCA matrix in Supplementary material C. Measured m/z values of the analyzed compounds are in good agreement with the theoretical values calculated for specific elemental compositions (noted differences from the theoretical mass, dtm is in all cases less than or equal to 10 mDa).</p><h4><strong>5. Conclusion</strong></h4><p>The combination of atmospheric solids analysis probe with high resolution tandem mass spectrometry proved to be a fast and effective tool for analysis of historical Asian lacquer objects, their resolution and classification. The developed method is based on profiling of phenol and benezenediols derivatives. The fragmentation of alkylphenols and alkylbenzene diols after collision activated dissociation in Trap and Transfer collision cells was described. Scission of alkyl chains and elimination of water represent the main fragmentation processes important for identification. Cyclic ion mobility separation appeared to be a useful tool for “cleaning” of signals of the lacquer ions from the matrix signals allowing their better identification from the fragmentation spectra. Moreover, ASAP-MS and MS/MS allowed detection of diterpenic resins, triterpenic resins and arsenic sulphide pigments present along with Asian lacquers. Principal Component Analysis provided complete separation of samples into three clusters of Asian lacquers in Score Plot. A simple classification approach based on direct comparison of newly suggested classification parameters (CPs) allows reliable differentiation of lacquer samples according to their provenance. The developed method is now implemented in routine controls of lacquered objects and could be applied to the analysis of resins and ambers obtained from archaeological findings.</p></div></div>

Characterization of Asian lacquers by atmospheric solids analysis probe high resolution tandem mass spectrometry coupled with cyclic ion mobility separation

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

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<div><h4><strong>Start Your Journey to ASTRA Expertise</strong></h4><p>Join us for the last <strong>5 series</strong> on building the perfect method for light scattering analysis.</p><p>Learn advanced tools and techniques to master ASTRA software, from normalization to DLS with flowing systems.</p><h4><strong>Session Overview:</strong></h4><ul><li><h5><strong>Part 6 of 10 Baselines &amp; Peaks - Best Practices &amp; Navigating Unexpected Chromatograms</strong></h5><ul><li>Thursday, September 11</li></ul></li><li><strong>Part 7 of 10</strong> Light Scattering Formalisms - Zimm, Mie, Berry, Debye, oh my! When and Why to Use Them<ul><li>Thursday, September 25</li></ul></li><li><strong>Part 8 of 10: </strong>Calculation Uncertainty and Error Analysis: Establishing Meaningful MALS Results<ul><li>Thursday, October 2</li></ul></li><li><strong>Part 9 of 10: </strong>Distribution Analysis &amp; Results Fitting: Advanced Peak Analysis Tools<ul><li>Thursday, October 9</li></ul></li><li><strong>Part 10 of 10:</strong> Mass Recovery &amp; Data Exporting: Effective Reporting of Results and Findings<ul><li>Thursday, October 23</li></ul></li></ul><h4><strong>Why Attend:</strong></h4><ul><li>Deepen your understanding of light scattering and ASTRA.</li><li>Gain actionable insights for your lab.</li><li>Interact with our expert, Dr. Christian Ackerschott.<br>&nbsp;</li></ul><blockquote><p><strong>Hosted by:</strong> Dr. Christian Ackerschott</p><p>Dr. Christian Ackerschott studied Chemistry at the University of Cologne and finished with his diploma thesis in Biochemistry. He completed his studies with the PhD thesis “Characterization of Recombinant Flagelliform Spider Silk Proteins” at the Technische Universität München, where AF4-MALS was used as an essential characterization method.</p><p>Since 2007, Christian works at Wyatt Technology as Application Scientist and is now Head of Customer Service. His tasks also include customer trainings in Dernbach and onsite as well as education and training of the local Field Application Scientists.</p></blockquote></div>

Waters Corporation: Interactive Web Series EU – Becoming a SEC-MALS & ASTRA Expert

Becoming a SEC-MALS & ASTRA Expert: Part 6

Learn to handle baseline shifts and tricky peaks in ASTRA. This session will help you troubleshoot and interpret unexpected chromatograms with confidence.

Becoming SEC-MALS & ASTRA Expert Part 6

<h4><strong>Overview</strong></h4><ul><li>During this presention, we will review the following:</li><li>What are GLP-1 receptor agonists</li><li>What challenges do they present to analysts</li><li>Semaglutide and related substances analysis</li><li>Semaglutide forced degradation studies</li></ul><h4><strong>Key Learning Points</strong></h4><ul><li>Identify key considerations when using reversed-phase chromatography for peptide analysis.</li><li>Learn about mobile phase and column considerations in method development</li><li>Explore strategies for optimizing gradients to account for sample complexity.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any analytical ccientists involved with GLP-1 receptor agonists analysis</li></ul><blockquote><p><strong>Speaker: </strong>Lucia Geis Asteggiante (Senior Technical Specialist, Phenomenex)</p><p>Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.</p></blockquote>

Phenomenex: Optimized Reversed Phase Analysis of Semaglutide

Optimized Reversed Phase Analysis of Semaglutide

Explore LC method development for GLP-1 agonists like semaglutide. Learn gradient tips, column choices, and degradation study.

<p><strong>Discover how advanced LC-MS/MS can deliver robust, low-maintenance performance for food contaminant testing, enabling faster results, high throughput, and reliable accuracy across challenging food matrices.</strong></p><p>As global food safety regulations become increasingly stringent, and consumer scrutiny intensifies, laboratories are pressured to deliver rapid, accurate results while managing complex sample matrices. Analysing emerging contaminants such as PFAS, pesticides, veterinary drugs, and other residues in processed or high-fat foods poses significant challenges in method robustness, instrument uptime, and data reliability. &nbsp;</p><p>This webinar will explore how advanced LC-MS/MS technologies are addressing these evolving demands. Through real-world case studies and application data, we will demonstrate how you can deliver exceptional performance with minimal sample prep. Learn how your lab can achieve: &nbsp;</p><ul><li>Reliable detection of contaminants across complex matrices &nbsp;</li><li>Greater operational efficiency with reduced instrument cleaning &nbsp;</li><li>Compliance with global food safety regulations &nbsp;</li><li>Lower cost per sample through enhanced system robustness &nbsp;</li></ul><p>As part of the webinar will be demonstrating the <strong>PerkinElmer QSight® 500 LC/MS/MS System</strong>, built to boost productivity and minimize maintenance with advanced technologies like self-cleaning ion sources, enhanced ion optics, and contamination-resistant design for uninterrupted performance. These advancements translate into longer maintenance intervals, higher throughput, and consistent data quality over thousands of injections, even with the most demanding food matrices. &nbsp;</p><p>Whether you’re testing for regulated residues or emerging threats, this session will provide actionable insights into optimizing your workflow for better efficiency and confidence in results. Don’t miss this opportunity to rethink your approach to food contaminant analysis. &nbsp;</p><blockquote><p><strong>Presenter:</strong> Jamie Foss (Product Manager, Liquid Chromatography &amp; Mass Spectrometry, PerkinElmer)</p><p>Jamie Foss is the Liquid Chromatography and Mass Spectrometry Product Manager at PerkinElmer. Prior to this role, he was a Sr. Application Scientist developing strategic applications in support PerkinElmer’s LC and LC/MS product portfolio. He has developed a wide variety of applications across food, environmental, industrial, forensics and cannabis. Prior to joining PerkinElmer in 2016, he spent 4 years as a forensic chemist for the State of Maine where he focused on the analysis of controlled substances and clandestine laboratory investigation.</p></blockquote>

PerkinElmer: Test More. Clean Less. Robust LC-MS/MS Solutions for Demanding Food Matrices

Test More. Clean Less. Robust LC-MS/MS Solutions for Demanding Food Matrices

Learn how advanced LC-MS/MS enables fast, reliable food contaminant testing with minimal prep and maintenance. Featuring PerkinElmer’s QSight® 500 system.

Robust LC-MS/MS Solutions for Demanding Food Matrices

<div><p><strong>Analytical methods are essential to ensuring pharmaceutical product quality, regulatory compliance, and patient safety. However, their reliability and reproducibility can be compromised by multiple sources of variability—including reagent quality, instrument performance, operator technique, sample preparation, and data processing. Understanding and controlling these sources is critical, especially when justifying post-approval changes to validated methods.</strong></p><p>This three-part webinar series features expert Peter Baker (Live Oak QA, Austin, Texas) and focuses on the importance of identifying, monitoring, and documenting variability within analytical workflows. Through open discussion and real-world insights, participants will gain a deeper understanding of how to build a scientifically sound case for method adjustments while maintaining regulatory compliance. Each session includes a live Q&amp;A to encourage interaction and address specific challenges.</p><h4><strong>Session 1: Controlling Analytical Variability to Justify Change and Ensure Compliance</strong></h4><p>To meet regulatory expectations, analytical methods must be scientifically justified, validated under real-world conditions, and supported by robust data. Numerous FDA Warning Letters issued post 2019 have included the requirement to vigilantly monitor inter- and intra-batch variability, but what should we be monitoring? This session explores how to identify critical sources of variability, interpret FDA warning letters, and avoid common compliance pitfalls. We will also discuss current guidance used to evaluate post-approval reporting expectations and their impact on product/patient.</p><p><i><strong>Goal: </strong>Learn how to generate a risk-based method monitoring strategy, with the goal of eventually demonstrating changes do not compromise product quality, safety, or efficacy.</i><br>&nbsp;</p><blockquote><p><strong>Presenter:</strong> Peter Baker (President, Live Oak Quality Assurance LLC)</p><p>Peter E. Baker, President, Live Oak Quality Assurance LLC, has been consulting nationally and internationally since April 2019. Prior to consulting, Peter spent 11 years as a U.S. FDA Drug Investigator, with most of those years spent working in FDA’s overseas offices located in India, China and Chile. Peter was named FDA Investigator of the Year in 2013 for his work uncovering serious breaches in data integrity and has special interest in data governance and compliance with 21 CFR Part 11. Live Oak focuses on training and employee empowerment in critical thinking, with the goal of improving the quality of medicines through good data management.</p><p><strong>Presenter: </strong>Dr. Stephanie Harden (Senior Manager, Small Molecule Core Solutions – LC-MS, Waters Corporation)</p><p>Stephanie Harden, Senior Manager at Waters Corporation, leads the Product Marketing and Scientific teams for Waters small molecule HPLC portfolio. With over 25 years of experience in product and segment marketing, she has a proven track record in strategic program development, cross-functional leadership, and market-driven innovation. Her work connects scientific insight with commercial strategy to drive customer-focused solutions and sustained business growth. Stephanie holds a PhD in Chemistry from the University of Bristol, UK, and is a widely cited author.</p></blockquote></div>

Waters Corporation: Managing Method Variability: A Foundation for Risk-Based Change

Managing Method Variability: A Foundation for Risk-Based Change - Session 1

Control method variability and meet FDA expectations. Discover risk-based strategies for analytical compliance in pharma with expert Peter Baker.

Analytical Variability and Regulatory Compliance

<div><p><strong>In today’s fast-paced biopharmaceutical landscape, researchers need tools that are not only powerful and precise but also intuitive and scalable. The DynaPro™ Plate Reader meets this demand by combining dynamic and static light scattering (DLS/SLS) in a high-throughput, user-friendly format.</strong></p><p>This webinar will explore how the instrument enables rapid, automated analysis of particle size, polydispersity, and colloidal stability.</p><p>Join us to gain a foundational understanding of high-throughput DLS and SLS techniques, along with practical insights into their application in biopharmaceutical workflows. We’ll also examine case studies that demonstrate how this advanced light scattering instrument supports the development of monoclonal antibodies (mAbs), lipid nanoparticles, and other therapeutic modalities.</p><h5><strong>Key Learning Objectives:</strong></h5><ul><li>Advantages of high-throughput DLS/SLS techniques</li><li>Fundamentals of dynamic and static light scattering (DLS/SLS)</li><li>Practical applications in stability screening of various therapeutic modalities, including mAbs and LNPs</li></ul><h5><strong>Who Should Attend:</strong></h5><ul><li>Curious minds interested in learning about the latest advancements in high-throughput analytical methods</li><li>Scientists across development, formulation, and quality control (QC) seeking to enhance their stability screening and aggregation assessment workflows</li><li>Laboratory managers looking to implement flexible, powerful technologies that are easy to integrate into existing lab environments</li></ul><blockquote><p><strong>Presenter:</strong> Richard Chung (Product Manager)</p><p>Richard Chung is a Product Manager at Wyatt Technology, bringing years of expertise in light scattering and analytical instrumentation. Since joining Wyatt in 2016, he has held key roles, including Application Scientist and Customer Support Manager, where he worked closely with researchers to solve complex analytical challenges. Now, as a Product Manager, Richard bridges the gap between scientists and better instruments—gathering insights from the field and collaborating with internal research teams to develop cutting-edge solutions that empower laboratories worldwide.</p></blockquote></div>

Waters Corporation: Simplicity and Speed: High-Throughput Stability Screening with Light Scattering Techniques

Simplicity and Speed: High-Throughput Stability Screening with Light Scattering Techniques

Learn how high-throughput DLS/SLS accelerates biopharma stability screening. See how the DynaPro™ Plate Reader supports mAb and LNP development.

High-Throughput Stability Screening with Light Scattering

<div><p><strong>Mass spectrometry continues to develop as a pivotal technique to ensure the safety and efficacy of the most advanced biotherapeutics and vaccines. In this session, you'll gain valuable insights into the characterization and quantification of complex biomolecules, and explore the role of high-resolution LC-MS in drug discovery, development, manufacturing and quality control. Discover how recent advancements in multi-reflecting time-of-flight mass spectrometry is shaping the future of biologics and deepening our understanding of these vital therapeutics.</strong></p><h5><strong>Who Should Attend:</strong></h5><ul><li>Learn about the operation of the novel Xevo MRT MS technology and see it in Action!</li><li>Discover how we unravel the complexity of peptide mapping workflows—including sequence mapping and host cell protein (HCP) analysis&nbsp;</li><li>Determine how multi-reflecting time-of-flight technology delivers quantitative and qualitative performance at fast acquisition speeds for biopharmaceutical workflows including oligonucleotide analysis&nbsp;</li></ul><p><i>The session will conclude with a live Q&amp;A where members of the Waters Xevo MRT team will be on hand to answer all of your questions.&nbsp;</i></p><blockquote><p><strong>Presenter:</strong> Jonathan Fox (Principal Applications Chemist, Waters Corporation)</p><p>Jon Fox is a principal applications chemist in the European Applications Laboratory team based in Wilmslow, UK. Jon joined Waters in December 2000, following on from his degree in drug design and toxicology, With over 20 years mass spectrometry experience supporting customers in the field with training, developing and troubleshoot their applications, now currently developing strategy, solutions and demonstrations in the European applications lab . His areas of expertise in include ion mobility, high resolution MS for analysis of oligonucleotides, proteomics, DMPK, APGC Food &amp; Environment and metabolomics.</p></blockquote></div>

Waters Corporation: XEVO MRT in Action - Demo Series

Mastering Biopharmaceutical Characterization

Discover how multi-reflecting TOF MS enhances biologics and vaccine analysis. Learn from real workflows and explore the power of Xevo MRT MS in LC-MS labs.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Did you know that the first charged aerosol detector (CAD) was introduced in 2005? Now 2 decades later, this near-universal detector is an essential part of the analytical chemist’s quantitative toolbox.</strong></p><p>Join Frank Steiner, PhD, and Paul Gamache, MS, one of the original inventors, for a webinar celebrating 20 years of HPLC-CAD technology. This seminar will cover the history of the invention, when the detector found a place for everyday use by separation scientists, and how the CAD compares to ELSD. More technical parts of the talk will showcase why detector settings like the evaporation temperature drive application versatility, and how the power function affects linearity and response curves.</p><h5><strong>Key Learning Objectives:</strong></h5><ul><li>Benefits of using charged aerosol detection for impurity analysis</li><li>Key parameters to get higher quantitative accuracy in your results</li><li>Experience new product developments by the inventors of charged aerosol detection technology</li></ul><h5><strong>Who Should Attend:</strong></h5><ul><li>Analytical chemists</li><li>Pharmaceutical scientists</li><li>Researchers</li><li>Quality control specialists</li><li>Industry professionals</li><li>Students</li></ul><blockquote><p><strong>Presenter: </strong>Frank Steiner, PhD (Senior Manager, Product Applications<br>Thermo Fisher Scientific)</p><p>Frank Steiner, PhD, is the senior manager of product applications and helps coordinate scientific collaborations at Thermo Fisher Scientific as a scientific advisor for HPLC. He received his PhD in analytical chemistry in 1995 from Saarland University in Saarbrücken, Germany, and has had more than 30 works published in refereed journals and more than 10 textbook chapters published on HPLC topics.</p><p><strong>Presenter:&nbsp;</strong>Paul Gamache, MS (Independent consultant)</p><p>Paul Gamache, MS, is a highly respected scientist, inventor, publisher, and technical consultant. His professional successes include the development of analytical technologies with a primary focus on charged aerosol detection and electrochemical detection for LC analysis, along with the design and launch of the first- and second-generation Corona CAD products.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Celebrating 20 years of HPLC-Charged Aerosol Detector technology

Celebrating 20 years of HPLC-Charged Aerosol Detector technology

20 years of HPLC-CAD. Discover CAD history, impurity analysis benefits, and technical insights with experts Frank Steiner and Paul Gamache.

20 years of HPLC-Charged Aerosol Detector technology

<div><h4><strong>How to make an LC method work on all LC systems</strong></h4><p>As an analyst, you often look at published LC-MS/MS methods from the literature, but the reported separation doesn't always work as described. You also know the importance of LC system configuration when developing a new method for production use. To bridge the gap between published methods and practical results, this webinar will feature Waters experts as they discuss system dwell volume, system dispersion, and how to transfer a method to another system with different column dimensions and particle sizes. They will also explain the theory behind the tool used for LC method transfer, providing a solid foundation for selecting the best column dimensions to maximize separation performance.</p><h5><strong>Who should attend?</strong></h5><ul><li>Clinical and Forensics scientists developing LC methods</li><li>Lab directors and managers.</li></ul><h5><strong>What you will learn:</strong></h5><ul><li>Key parameters to consider when transferring a method successfully—without the need to re-develop it through trial and error.</li><li>How to calculate and adjust gradient step volumes to compensate for differences in system dwell volume.</li><li>Practical examples demonstrating method transfer across systems.&nbsp;</li></ul><blockquote><p><strong>Presenter:</strong> Guy Boisvert (Clinical and Forensics Chemistry Account Manager, Waters)</p><p>With more than 28 years of experience at Waters, in different roles, Guy is providing some guidance in chemistry product selection and support after sales. His goal is to minimize your valuable time in method development by selecting the best product first. Guy is presenting on topics and specific aspects that are important to you.</p></blockquote></div>

Waters Corporation: Chemistry Hour Series with Guy Boisvert: Pre-analytical challenges Part 2

Chemistry Hour Series with Guy Boisvert: Pre-analytical challenges Part 2

Make LC methods work on any system. Learn key parameters, dwell volume adjustments, and method transfer strategies with Waters’ expert guidance.

Chemistry Series Guy Boisvert: Pre-analytical challenges

<p><strong>The Karl Fischer (KF) method is a globally trusted technique for determining water content across a wide range of sample types. While the procedure is routine in many labs, chemical testing laboratories face a unique challenge: analyzing an ever-changing array of unknown and complex matrices.</strong></p><p>Join us to learn how one of the world’s largest chemical testing laboratories tackles complex moisture analysis. Krystal Heffelfinger, an experienced chemistry technician at Intertek, will share challenges she encounters when analyzing everything from industrial chemicals, adhesives, and lubricants to pharmaceuticals, food ingredients, and personal care products. With such a wide sample scope, each day brings a new moisture determination puzzle.</p><p>Eduardo Simões, Titration Product Specialist at Metrohm USA, will demonstrate how KF automation helps streamline workflows, improve consistency, and boost throughput for labs managing high sample volumes.</p><p>Stay for the live Q&amp;A session, where Krystal and Eduardo will answer your questions and share practical insights for tackling difficult samples. Attendance will be recognized with a certificate you can add to your professional development portfolio.</p><h5><strong>This mini course is ideal for:</strong></h5><ul><li>Those interested in learning the fundamentals and nuances of Karl Fischer titration</li><li>Labs that routinely deal with unknown or complex samples for moisture analysis</li><li>Teams seeking to improve moisture determination workflows through automation</li><li>Anyone curious about how the KF Oven technique applies to different sample types</li></ul><h5><strong>What you'll learn</strong></h5><ul><li>How to choose the right KF technique (volumetric vs. coulometric) for your sample</li><li>Smart sample prep strategies to boost accuracy and repeatability</li><li>Tips on selecting the right sample amount and handling procedures</li><li>How to analyze water in tough matrices — and when to use the KF Oven for better results</li><li>Where automation can make the biggest impact in high-throughput labs</li></ul><blockquote><p><strong>Presenter: </strong>Krystal Heffelfinger (Analyst Technician, Intertek)</p><p>Krystal Heffelfinger joins us from the Inorganic Analysis team at Intertek Allentown (PA), bringing valuable expertise in analytical testing. In addition to her broad testing responsibil-ities, she specializes in a range of Metrohm instrumentation and techniques. With two years of hands-on experience, Krystal has focused on method development and analysis using volumetric and coulometric Karl Fischer titration, potentiometric titration, and pH measurement across a variety of sample types and industries. She holds a Bachelor of Science in Forensic Chemistry from Lock Haven University, equipping her with a solid foundation for tackling the complex challenges of unknown sample characterization in third-party testing.</p><p><strong>Presenter:&nbsp;</strong>Eduardo Simões (Titration Product Specialist, Metrohm USA)</p><p>As the Titration Product Specialist at Metrohm USA in Riverview, Florida, Eduardo Simoes, MSc, leverages 22 years of practical experience in potentiometry and electroanalysis. His expertise spans instrument installations, technical support, and training, gained through extensive field work. He holds bachelor's and master's degrees in analytical chemistry from the University of Campinas, Brazil, providing a strong theoretical foundation for his hands-on approach.</p></blockquote>

Metrohm: Karl Fischer in Action: Moisture Analysis in a High-Throughput Testing Lab

Karl Fischer in Action: Moisture Analysis in a High-Throughput Testing Lab

Learn advanced Karl Fischer titration strategies for complex samples. Intertek and Metrohm experts share tips on automation, sample prep, and KF Oven use.

Karl Fischer Moisture Analysis in a Testing Lab

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Extracellular vesicles (EVs), including exosomes and microvesicles, are membrane-bound particles secreted by virtually all cell types. They are now recognized as critical mediators of intercellular communication, capable of transferring proteins, lipids, and nucleic acids between cells. This capacity positions EVs as key players in numerous physiological and pathological processes, including immune responses, tissue repair, neurodegeneration, and cancer progression.</strong></p><p>The study of EVs holds immense promise for the development of novel diagnostic and therapeutic strategies, particularly targeted drug delivery. Despite significant advances, challenges remain in standardizing EV isolation and characterization methods. Continued research is essential to fully elucidate their biological roles and to harness their potential in clinical applications.</p><p>From Agilent, we are committed to improvement: Join us on our free webinar dedicated to the analysis of extracellular vesicles (EVs) and exosomes in flow cytometry.</p><p>From our conventional systems to our New Spectral instrument, you'll discover how to take advantage of the advanced performance and enhanced sensitivity of NovoCyte cytometers to obtain reproducible and reliable results in your small particle studies.</p><h5><strong>We'll be covering the following:</strong></h5><ul><li>The technical challenges of analyzing extracellular vesicles by flow cytometry</li><li>How to set up an efficient and standardized workflow with the NovoCyte family</li><li>Present some applications.</li><li>Understanding EVs not only deepens our insight into cellular communication but also opens new frontiers in precision medicine.</li></ul><h5><strong>Who should attend:</strong></h5><ul><li>Biomedicine researchers</li><li>clinical researchers</li><li>lab technicians</li><li>any pre-doc or post-doc</li><li>anyone who is interested on improving their flow cytometry workflow regarding extracellular vesicles studies</li></ul><blockquote><p><strong>Presenter: </strong>Camille Santa-Maria (Flow Cytometry Field Application Scientist)</p><p>Camille has been Field Application Scientist at Agilent Technologies for nearly six years, providing support for flow cytometry across Europe. She has extensive experience with both conventional and spectral flow cytometry, working with a wide range of sample types, including human and murine specimens, seawater, and bacteria. Prior to joining Agilent, she worked on a cytometry platform, where she specialized in immunophenotyping in mouse models.</p></blockquote></div></div></div>

Agilent Technologies: Implement Your Flow Cytometry Workflow: Extracellular Vesicles and Exosomes with the NovoCyte Family

Implement Your Flow Cytometry Workflow: Extracellular Vesicles and Exosomes with the NovoCyte Family

Join Agilent’s webinar on EV and exosome analysis with NovoCyte flow cytometry. Discover workflows, applications, and tools for reproducible, reliable results.

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