<p><strong>Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.</strong></p><h4><strong>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</strong></h4><p>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.</p><p>So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.</p><div><div><p>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.</p><p>At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (<strong>Figure 1</strong>).</p><p>Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493"></p><p>While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.</p><p>And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.</p><h4><strong>Key takeaways</strong></h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4><strong>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</strong></h4><p>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.</p><p>Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.</p><p>Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.</p><h4><strong>Online SPE analysis provides key benefits like:</strong></h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul><p>Let’s explore what this technique looks like in practice.</p><h4><strong>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</strong></h4><p>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (<strong>Figure 2</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"></p><h4><strong>The process for analyzing a hops standard solution is straightforward:</strong></h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"><strong> on the SPE column</strong></a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"><strong> analytical column </strong></a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (<strong>Figure 3</strong>).</li></ol><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"></p><h4><strong>Online SPE for analyzing α- and β-bitter acids in real hops samples</strong></h4><p>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.</p><p>To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.</p><p>The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (<strong>Figure 4</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"></p><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4><strong>From carefully procured samples to tasty sips</strong></h4><p>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.</p><ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul><p>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf"><strong>check out our application note today</strong></a>.</p><h4><strong>FAQs</strong></h4><div><div><h5><strong>What are the key compounds in hops analyzed by HPLC?</strong></h5><p>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</p></div><div><h5><strong>What column types are recommended for hop acid separations?</strong></h5><p>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</p></div><div><h5><strong>What’s the best workflow for hops analysis?</strong></h5><p>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #Automation</p></blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

Webinars LabRulezLCMS Week 27/2026

<p><strong>The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;</strong></p><p>There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607"></p><p>A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;</p><h4><strong>Evaluation of measurement procedure performance&nbsp;</strong></h4><p>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;</p><ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"></p><h4><strong>Characterisation of candidate reference materials&nbsp;</strong></h4><p>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;</p><ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4><strong>Other ILCs&nbsp;</strong></h4><p>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).</p>

Interlaboratory comparisons other than proficiency testing

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Shimadzu Corporation

<p><strong>When developing an analytical HPLC method, our goal is the identification and quantitation of a compound of interest.</strong></p>
<p>To achieve this goal we optimize parameters like baseline resolution, peak symmetry and peak width. But in preparative HPLC method development, our goal is the separation and isolation of a valuable compound. The analytical chromatographic parameters become less important—throughput, yield and purity of the compound are the more important parameters for preparative HPLC.</p>
<p>In this webinar, we will discuss the process of scaling up from an analytical to a preparative HPLC method. We will look at some key parameters and calculations as well as column and instrumentation considerations.</p>
<blockquote>
<p><strong>Presenter:</strong> Mark Powell (Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.</p>
</blockquote>


Agilent Technologies: Getting Off to a Good Start: Preparative Method Development Strategies

Getting Off to a Good Start: Preparative Method Development Strategies

The process of scaling up from an analytical to a preparative HPLC method.

Preparative Method Development Strategies

<p><strong>When creating a Preparative Chromatography method, the concerns is the quantity of the crude material, its impurities, and the resources that are available. Preparative separations become an economic decision as we balance the cost of solvents, adsorbents and time. What is most important will weigh in deciding which column and method we want to use.</strong> </p>
<p>In this presentation the focus will be on three of the most common purification challenges today; that of peptides, oligonucleotides, and the high throughput small molecules. Whether you have a lot of one compound to purify or small amounts of many compounds to purify, different tactical approaches will be discussed as to how to achieve your purification objectives.</p>
<blockquote>
<p><strong>Presenter: Carl Griffin (LC Column Applications Scientist, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Carl Griffin started his career as an Organic Chemist working at the Kilo scale in a pharmaceutical company where he got his first exposure to preparative chromatography. Before joining Agilent in 2010, he worked with manufacturers of preparative and process chromatography instrumentation and media including Polymer Labs (now apart of Agilent). He has worked on projects from mgs to kgs, from small molecules to the largest biomolecules.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Paul Dinsmoor (Large Molecule Applications Scientist, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Paul Dinsmoor is a Large Molecule Field Applications Scientist with Agilent Technologies in La Jolla, CA. He has a Biochemistry &amp; Cell Biology degree from UC San Diego with over 30 years of bio chromatography experience which includes 15 years as a bioanalytical chemist in research and development at La Jolla Pharmaceutical &amp; Pfizer as well as 14 years as a field application scientist at Polymer Labs/ Varian/ Agilent focusing on BioHPLC analysis and macromolecular characterization. At Agilent Paul helps customers with column recommendations, method development, and troubleshooting.</p>
</blockquote>


Agilent Technologies: Practical Approaches to Preparative Chromatography

Practical Approaches to Preparative Chromatography

<p><strong>Please join us for this webinar where we will present an introduction to Agilent’s InfinityLab Purification Solutions with an introduction to Preparative Chromatography discussed from an analytical HPLC to Preparative HPLC perspective.</strong></p>
<p>We will discuss the theory of preparative chromatography, the components of a typical preparative HPLC system and discuss some strategies for scale up from analytical methods. We will present some of the common challenges facing Prep Chromatographers as they apply the theory of scale up to practical laboratory applications. </p>
<p>Our case study will focus on an analytical setup of a preparative HPLC system. We look forward to sharing an introduction into the basic principles of preparative liquid chromatography while providing some practical solutions for today’s chemists. Collect each and every drop!</p>
<blockquote>
<p><strong>Presenter: Lori Sandford (HPLC Applications Engineer, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Lori Sandford is an HPLC Applications Scientist with Agilent Technologies, Inc. in Wood Dale, IL. Lori has been working at Agilent Technologies supporting HPLC instrumentation since 2010, including 2 years with Varian working as an HPLC Specialist. Prior to Agilent, she was the Global Technology Manager for Prep and Process Chromatography at W. R. Grace/Alltech. Her career started at G.D. Searle/Pfizer in 1998 as a chromatography specialist in the Prep and Process Chromatography group supporting both Medicinal and Process Separations. Lori attended Florida State University where she received her degree in Chemistry. When not tinkering in the lab, Lori is enjoys time at home with her 2 teenage boys shuttling from hockey rink to Lacrosse field, enjoying concerts or planning her next scuba adventure.</p>
</blockquote>
<blockquote>
<p><strong>Presenter: Regina Black (LC Applications Scientist, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Gina joined Agilent over 2 years ago and brought with her a wealth of experience in the pharmaceutical industry. She began her career at Merck as a synthetic chemist and became a subject matter expert in preparative HPLC, eventually joining the Separations COE, supporting both discovery and process development efforts. After twenty-two years at Merck, Gina moved to Bristol-Myers Squibb where she served as a site lead for their Single Compound Purification group, using a highly automated workflow to analyze, purify and deliver final products for biological testing. Gina then moved back into large scale separations and enabling technologies supporting Chemical Development at BMS. Over the years, Gina has developed an extensive background in both reverse phase and normal phase HPLC, SFC and IEX methodology.</p>
</blockquote>


Agilent Technologies: “A” is for Analytical to Preparative Chromatography

“A” is for Analytical to Preparative Chromatography

<p><strong>Please join us for this webinar where we will take an in-depth tour of Agilent’s InfinityLab Purification Solutions and the wide range of available fraction collection and triggering options with multiple detectors such as UV, MS and ELSD.</strong></p>
<p>We will include guidance on configuring the appropriate fraction collector for your workflow as well as provide a thorough overview of the fraction collection parameters in OpenLab CDS Chemstation Edition. There will be plenty of helpful tips and tricks on the application of these settings to tackle challenging situations, ensuring optimal recovery and selectivity in all your purification workflows. Collect each and every drop!</p>
<blockquote>
<p><strong>Presenters: Regina Black (LC Applications Scientist, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Gina joined Agilent over 2 years ago and brought with her a wealth of experience in the pharmaceutical industry. She began her career at Merck as a synthetic chemist and became a subject matter expert in preparative HPLC, eventually joining the Separations COE, supporting both discovery and process development efforts. After twenty-two years at Merck, Gina moved to Bristol-Myers Squibb where she served as a site lead for their Single Compound Purification group, using a highly automated workflow to analyze, purify and deliver final products for biological testing. Gina then moved back into large scale separations and enabling technologies supporting Chemical Development at BMS. Over the years, Gina has developed an extensive background in both reverse phase and normal phase HPLC, SFC and IEX methodology.</p>
</blockquote>
<blockquote>
<p><strong>Presenters: Lori Sandford (HPLC Applications Engineer, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Lori Sandford is an HPLC Applications Scientist with Agilent Technologies, Inc. in Wood Dale, IL. Lori has been working at Agilent Technologies supporting HPLC instrumentation since 2010, including 2 years with Varian working as an HPLC Specialist. Prior to Agilent, she was the Global Technology Manager for Prep and Process Chromatography at W. R. Grace/Alltech. Her career started at G.D. Searle/Pfizer in 1998 as a chromatography specialist in the Prep and Process Chromatography group supporting both Medicinal and Process Separations. Lori attended Florida State University where she received her degree in Chemistry. When not tinkering in the lab, Lori is enjoys time at home with her 2 teenage boys shuttling from hockey rink to Lacrosse field, enjoying concerts or planning her next scuba adventure.</p>
</blockquote>


Agilent Technologies: “F” is for Fractionation: Advanced Topics in Fraction Collection using Agilent’s InfinityLab Purifcation Solutions

“F” is for Fractionation: Advanced Topics in Fraction Collection using Agilent’s InfinityLab Purifcation Solutions

<h3><strong>This first session will cover:</strong></h3>
<ul>
<li>What is LC purification and how do you get the most out of the technique. </li>
<li>How much sample can you expect to purify?</li>
<li>Up-scaling basics</li>
<li>Recycling and concentration</li>
</ul>
<p>This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more. </p>
<h3><strong>Future sessions will aim to cover the following topics:</strong></h3>
<ul>
<li>Semi-Micro to Analytical Scale LC Purification</li>
<li>Semi-Preparative and other LC Purification Techniques</li>
</ul>


Shimadzu: LC Purification Series - Session 1: Fundamentals of LC Purification

LC Purification Series - Session 1: Fundamentals of LC Purification

<h3><strong>This second session will cover:</strong></h3>
<ul>
<li>Differences between Semi-micro and Analytical Scale Prep</li>
<li>Principles of the techniques</li>
<li>Things to consider in semi-micro and analytical scale purification</li>
<li>Instrumentation and software advantages</li>
</ul>
<p>This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more. </p>
<p>We recommend viewing Session 1 before attending this webinar.</p>
<h3><strong>Future sessions will cover:</strong></h3>
<ul>
<li>Semi-Preparative and other LC Purification Techniques</li>
</ul>


Shimadzu: LC Purification Series - Session 2: Semi-Micro to Analytical Scale LC Purification

LC Purification Series - Session 2: Semi-Micro to Analytical Scale LC Purification

<h3><strong>This session will cover:</strong></h3>
<ul>
<li>Principles, common issues and tips and tricks of Semi-Preparative Chromatography</li>
<li>UFPLC – Ultra Fast Preparative and Purification LC</li>
<li>Semi-preparative SFC Scale purification</li>
</ul>
<p>This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more. </p>
<p>We recommend viewing before attending this webinar: </p>
<ul>
<li>Sessions 1</li>
<li>Sessions 2</li>
</ul>


Shimadzu: LC Purification Series - Session 3: Semi-Preparative and other LC Purification Techniques

LCMS webinars focusing on PrepLC - page 7