<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

<h3>We're excited to announce that <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity Chromatography Software</a> version 10.1.2 is now available!</h3>This minor update expands Clarity’s portfolio with new control modules.<h4>Newly added Instrument control modules</h4><ul><li>Ecom auxiliary modules</li><li>HTA ICF control modules</li><li>Spark pump</li></ul><h4>Updated Instrument control modules</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/168">Ecom</a> HPLC</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/328">Knauer</a> HPLC</li><li>Spark ICF control modules</li><li>YoungIn Chromass HPLC</li></ul><blockquote>For a complete list of module changes, see the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">Changelog</a>. For more info about exciting new features directly in Clarity, explore what was introduced in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5622">Clarity 10.1 release highlights</a>.Clarity can control 1000+ instruments; the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/controls">list can be previewed here</a>.</blockquote><h4>Supported Instruments in Clarity (DataApex) — Quick Guide</h4>Clarity Chromatography Software supports a wide range of chromatographs and peripherals via digital control (DD) and analog acquisition (A/D) using the Colibrick module.<h5>Gas Chromatographs (GC)</h5>Digital integration over LAN/RS232 (many via ICF):<ul><li>Agilent: 5890, 6890, 7890, 8860, 8890, Intuvo 9000</li><li>Shimadzu: GC-2010/2014, Nexis GC-2030</li><li>Thermo Fisher: TRACE 1300/1310</li><li>PerkinElmer: Clarus 400–690</li><li>Also supported: Dani, Ellutia, Young In Chromass, Apix, Fuli, GOW-MAC (selected models)</li></ul><blockquote>Note: Most Agilent 6890–8890 and Intuvo use LAN – ICF.</blockquote><h5>HPLC Systems</h5>Full system/module control:<ul><li>Agilent 1100–1290 Infinity II (ICF)</li><li>Shimadzu LC-10/20 and Nexera LC-40</li><li>PerkinElmer Flexar / NexSAR</li><li>Knauer Azura / BlueShadow / Smartline</li><li>Hitachi Chromaster / Primaide</li><li>Young In Chromass ChroZen series</li><li>Sykam S500–S600, Watrex Streamline, Hanon LC7000–7300</li></ul><h5>HPLC Modules (Pumps, Detectors, Ovens, Fraction Collectors)</h5><ul><li>Pumps: Agilent 1100–1290 families, Knauer, Shimadzu, PerkinElmer, Sykam, SSI, Ecom</li><li>Detectors: VWD/DAD(PDA)/RID/FLD/ELSD across Agilent, Shimadzu, Knauer, PerkinElmer, Young In Chromass, Ecom, etc.</li><li>Column Ovens: Agilent G1316x / G7116x, Shimadzu CTO-20/30/40, Knauer CT2.1, PerkinElmer Flexar</li><li>Fraction Collectors: Knauer FC 6.1, Teledyne FOXY series, Ecom ECF 2096, Waters Fraction Collector II</li></ul><h5>Autosamplers</h5><ul><li>Agilent GC ALS (7673/7683/7693/8697), HPLC ALS modules (G1313A–G7167C)</li><li>Shimadzu AOC-20 / AOC-5000+, SIL-10 / SIL-20 / SIL-40 series</li><li>CTC Analytics PAL, HTA autosamplers, Spark Alias/UHPLC, Knauer AS 6.1L</li><li>PerkinElmer Flexar / NexSAR AS</li></ul><h5>Other Devices</h5><ul><li>GC×GC modulators (e.g., Charles University GC×GC Modulator)</li><li>Valves &amp; switch modules (Agilent, Knauer, Shimadzu, VICI-Valco)</li><li>Degassers, flow monitors, managers, interfaces</li><li>LC-MS / GC-MS: Advion Expression CMS, Microsaic 4500 MiD, Markes BenchTOF (and others)</li><li>Balances (EA extension): Mettler Toledo, Sartorius</li></ul><h5>Analog Acquisition (Colibrick)</h5>A/D support for legacy or analog-output devices:<ul><li>GC: Agilent 5880/4890A, Varian 3xxx/3800/3900, PerkinElmer Clarus series, Thermo TRACE, Shimadzu GC-14/9A, Scion 43x/45x/83xx/85xx, etc.</li><li>HPLC Detectors: Waters 2487/2489, ESA Corona, Hitachi L-series, JASCO FP/UV, Ecom LCD series, many others</li><li>Elemental &amp; Amino Acid Analyzers: Carlo Erba/Costech ECS, Biochrom 20/30+</li></ul>If you are interested in a DEMO VERSION, contact us!

DataApex Clarity Chromatography software 10.1.2 just released!

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<div>High-throughput proteomics is pushing laboratories to generate deeper, more reliable data from smaller sample amounts without sacrificing speed or consistency. As workflows shorten, questions remain around maintaining quantitative accuracy and cross-lab reproducibility.This webinar examines how recent advances in liquid chromatography-mass spectrometry (LC-MS) are addressing these challenges through insights from a large multi-laboratory benchmark study using standardized proteome samples. Attendees will see how next-generation approaches can support robust, reproducible high-throughput proteomics and how publicly available benchmark datasets are helping the community evaluate and improve data analysis strategies.<h4>Attend this webinar to:</h4><ul><li>Understand how short gradient LC–MS workflows support high throughput label-free quantitation (LFQ) proteomics</li><li>Explore the role of data independent acquisition strategies in improving proteome coverage and quantitative reproducibility</li><li>Learn from a multi laboratory benchmark study using standardized proteome samples</li><li>Learn why the ZenoTOF 8600 system is perfectly positioned to support high-throughput LFQ proteomics&nbsp;</li></ul><blockquote>Presenter: Bart Van Puyvelde, PhD (Field Application Specialist, SCIEX)Dr. Bart Van Puyvelde is a field application specialist at SCIEX, a role he recently stepped into after completing his postdoctoral research at the Laboratory of Pharmaceutical Biotechnology at Ghent University, Belgium.</blockquote></div>

Technology Networks: Label Free Quantitation: New Insights Into High-Throughput Proteomic Workflows

Label Free Quantitation: New Insights Into High-Throughput Proteomic Workflows

Webinar on high-throughput LFQ proteomics using LC-MS and ZenoTOF 8600, highlighting reproducibility and multi-lab benchmark results.

Insights Into High-Throughput Proteomic Workflows

In biotherapeutic development, speed and confidence in impurity profiling are critical to moving candidates forward. This webinar introduces a streamlined workflow designed to accelerate impurity profiling of GLP-1 analogs. It begins with impurity screening using the new Pro IQ Plus single quadrupole mass detector. Then, detailed sequence analysis is performed using the 6545XT AdvanceBio LC/Q-TOF, equipped with an ExD cell to enable electron capture dissociation (ECD). ECD offers a gentler fragmentation approach than traditional CID, preserving labile modifications and enabling detailed structural insights.&nbsp;We highlight key advantages of ECD including modification localization, isoaspartate investigation, and discuss the importance of spectral quality in sequence confirmation. Whether you're optimizing GLP-1 analogs or exploring new biotherapeutics, this webinar highlights how Agilent’s tools can help get answers faster and go deeper with your data.<blockquote>Presenter: Rachel Franklin (Application Development Scientist, Agilent Technologies, Inc.)Rachel is an Application Development Scientist at Agilent Technologies who specializes in developing innovative applications using high resolution mass spectrometry. She joined Agilent in 2023 through the acquisition of e-MSion, the developer of the core technology for the Agilent ExD cell. Rachel earned her PhD in Biochemistry and Biophysics from Oregon State University where she focused her research on studying genetically engineered protein systems using top-down mass spectrometry. In her current role at Agilent, Rachel continues to advance research in this area and enjoys exploring new application areas.</blockquote>

Agilent Technologies: Accelerating GLP-1 Analog Impurity Profiling with the Agilent Pro IQ Plus Followed by Detailed Sequence Analysis using Electron Capture Dissociation

Accelerating GLP-1 Analog Impurity Profiling with the Agilent Pro IQ Plus Followed by Detailed Sequence Analysis using Electron Capture Dissociation

Fast GLP-1 impurity profiling using Pro IQ Plus and 6545XT LC/Q-TOF with ExD ECD for confident sequence analysis.

GLP-1 Analog Impurity Profiling with Agilent Pro IQ Plus

<h4>Join us for a free webinar on Analysis of Inositol Stereoisomer Profiling in Dietary Supplements and Foods</h4>Inositol plays a key role in regulating numerous metabolic pathways and hormonal signaling processes. Maintaining a balanced profile of inositol stereoisomers in the human body is critical for supporting key biological functions and may help reduce the risk of certain health disorders. The aim of this webinar is to present analytical procedures capable of simultaneously separating and quantifying free inositol stereoisomers in both simple matrices, such as dietary supplements, and complex matrices, such as milk, cereal, and infant formula. An existing Hydrophilic Interaction Liquid Chromatography (HILIC) protocol was systematically optimized to achieve adequate resolution and reliable separation of seven inositol stereoisomers (allo-, muco-, epi-, D-chiro-, neo-, myo-, and scyllo-inositol) using a UPLC™ BEH™ Amide Column. Two different sample preparation procedures and two mass spectrometry (MS) techniques (ACQUITY™ QDa™ II Mass Detector and Xevo™ TQ-S cronos Mass Spectrometer) were developed for the analysis of the simple and complex matrices, respectively. Details of method development and analytical performance will be discussed.<blockquote>Presenter: Jinchuan Yang, Ph.D. (Principal Scientist, Waters Corporation)Dr. Jinchuan Yang is a Principal Scientist Waters Corporation, and a subject matter expert in food and environmental applications, polymer chromatography, and LC and MS technologies. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, market development, and support for R&amp;D and training. He is an active participant in technical stakeholder groups at AOAC International and USP, serving as an expert panel member in AOAC SPIFAN and participating in review, recommendation and muti-lab testing of AOAC methods.Presenter:&nbsp;Dimple Shah (Moderator | Principal Food and Materials Market Development Manager, Waters Corporation)Dimple Shah has a M.S. degree in Organic Chemistry from St. Xavier’s college, Gujarat University, India. She also has a M.S. degree in Chemistry from University of Massachusetts at Lowell. She is working at Waters Corporation, Milford as a Principal Market Development Manager for Food and Material Science. In her previous journey she was a Principal Scientist at Waters and worked in Genotoxic impurity and Food and Environment market. She was responsible for development of analytical methods for contaminants in food and water using LC and Mass spectrometers. Her areas of expertise are LC, tandem quadruple, high resolution mass spectrometry and various chromatographic and mass spectrometry software. She has published several application notes on polar pesticides, pesticides, veterinary drugs, mycotoxins, sugars, sweeteners, vitamins, dyes and NDSRIs.</blockquote>

Waters Corporation: Fit-for-purpose workflows for inositol stereoisomer profiling in dietary supplements and foods using single and tandem mass spectrometry

Fit-for-purpose workflows for inositol stereoisomer profiling in dietary supplements and foods using single and tandem mass spectrometry

Analytical procedures capable of simultaneously separating and quantifying free inositol stereoisomers in simple and complex matrices.

Inositol Profiling by Single & Tandem Mass Spectrometry

Mass spectrometry is ideally suited to the analysis of trace explosive contamination, offering the sensitivity and selectivity required to detect very small quantities of organic explosive materials. Over the past 20 years, liquid chromatography paired with triple‑quadrupole and high‑resolution mass spectrometry has become the mainstay of forensic laboratories, gradually replacing GC and GC–MS for the analysis of many explosive compounds.At the same time, ion mobility spectrometers (IMS) have become the dominant technology for explosives threat detection (ETD) in security environments, ensuring that airports and other vulnerable sites can rapidly screen for illicit trace residues.Recently, the Waters RADIAN™ ASAP mass spectrometer has gained popularity for rapid, targeted screening of illicit drugs in law‑enforcement workflows worldwide. This presentation will explore how the capabilities of the RADIAN system can be extended to the identification of explosive materials across a range of scenarios. It will also consider how the instrument can be used to model ions generated by explosives to support the development of new detection technologies, and ultimately how mass spectrometry could contribute to future security‑screening applications.<blockquote>Presenter: Dr. Patrick Sears (Senior Lecturer of Chemistry and Forensic Analysis, School of Chemistry and Chemical Engineering, University of Surrey)Dr. Patrick Sears is a Senior Lecturer of Chemistry and Forensic Analysis at the University of Surrey in the School of Chemistry and Chemical Engineering. His research expertise and background include developing analytical chemistry solutions for complex problems and understanding how analytical systems can be broken down to improve precision and accuracy. Patrick chairs the ambient ionisation interest group of the British Mass Spectrometry Society and his main research focus is how these technologies can be used to address a diverse range of applications including Drugs of Abuse, Explosives, Environmental Pollutants and Pesticides.Dr. Sears received his B.Sc. (1995) and M.Sc. (1996) from the University of Durham before completing a Ph.D. at the University of Hull completing research on the catalytic oxidation of isobutene by uranium oxide. After being awarded his PhD, Dr. Sears worked in process safety analysis (at Rhodia CSD and Thermal Hazard Technology) and in reaction optimisation and flow chemistry (at Syrris) before joining the defence science and technology laboratory (Dstl) in 2008. At Dstl, Dr. Sears was a team leader in the Forensic Explosives Laboratory and latterly a Principal Scientist in Explosives Detection where his research interests included the analysis of trace explosive contamination and residues.</blockquote>

Waters Corporation: RADIAN™ ASAP Mass Spectrometry for the Detection and Analysis of Explosive Traces

RADIAN™ ASAP Mass Spectrometry for the Detection and Analysis of Explosive Traces

Trace explosive detection by mass spectrometry, RADIAN™ ASAP screening workflows, and future security applications in forensic and threat-detection labs.

RADIAN™ ASAP MS for Explosive Traces analysis

Join this webinar to hear from Lorena Manzano as she highlights the benefits of ZenoTOF 7600 system for the analysis of pharmaceuticals and pesticides in wastewater, soils and crops.This webinar will provide an overview of advanced high-resolution mass spectrometry (HRMS) strategies for monitoring pharmaceuticals and pesticides in complex environmental and agricultural matrices. Lorena Manzano will share recent findings on the occurrence, persistence, and potential transfer of these contaminants from reclaimed water to soils and crops, highlighting how innovative analytical solutions can support safer water reuse practices.Water scarcity and the increasing use of reclaimed water for irrigation create both opportunities and challenges for agriculture. While reuse contributes to sustainability, it also introduces chemical contaminants into the food chain. Conventional monitoring approaches often fail to provide the sensitivity, selectivity, and robustness required to detect low-level residues in highly complex samples such as soils and edible plant tissues.This webinar will address these challenges by discussing the role of HRMS, with a particular focus on how ZenoTOF 7600 system enhances confidence in compound identification and quantification. Attendees will learn about key trends in environmental risk assessment, the importance of tracking persistent pharmaceuticals such as carbamazepine, and the role of multi-residue analysis in ensuring food safety.Case studies from greenhouse experiments will be presented, demonstrating real-world examples of pharmaceutical and pesticide transfer from wastewater to soils and edible crops. Finally, the session will provide actionable insights into how cutting-edge HRMS can be integrated into monitoring programs to better protect both agricultural productivity and public health.<h4>Key learning points:</h4><ul><li>Understand the occurrence and persistence of pharmaceuticals and pesticides in reclaimed water, irrigated soils, and edible crops.</li><li>Explore how ZenoTOF 7600 system enhances detection, identification, and quantification of contaminants in complex matrices.</li><li>Learn from greenhouse case studies on pharmaceutical and pesticide accumulation under real-world irrigation practices.</li><li>Identify key challenges and opportunities for safe wastewater reuse in agriculture and food safety monitoring</li></ul><blockquote>Speaker: Lorena Manzano (PhD in Analytical Chemistry, University Almería, Spain)Lorena is an expert in advanced chromatographic and mass spectrometric techniques. With over six years of experience at the European Union Reference Laboratory for Fruits and Vegetables (EURL-FV), her work has focused on the application of LC- and GC-based high- and low-resolution mass spectrometry for the monitoring of pesticide residues in plant-derived matrices.</blockquote>

SCIEX: Tracking irrigation-related contaminants in water and soils (QTOF-MS)

Tracking irrigation-related contaminants in water and soils (QTOF-MS)

Benefits of ZenoTOF 7600 system for the analysis of pharmaceuticals and pesticides in wastewater, soils and crops.

QTOF-MS Tracking contaminants in water and soils

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4>In situ native proteomics: Imaging and top-down analysis of intact protein complexes in tissue</h4>Join this webinar to hear about latest methods and advances for probing intact protein complexes in tissue using in situ native mass spectrometry.Native ambient mass spectrometry (NAMS) enables the study of proteins and their complexes directly in tissue. The latest developments in NAMS will be presented, including innovative MS imaging and on-tissue native top-down MS workflows.Topics will include:<ul><li>Analysis of membrane proteins</li><li>Identification of protein-metal complexes implicated in neurodegenerative disease.</li></ul>Cannot join on April 16? Please register anyway to automatically receive access to the recording.<blockquote>Presenter: Dr. Oliver Hale (Senior Postdoctoral Research Fellow, University of Birmingham)Dr. Oliver Hale is a senior postdoctoral research fellow in the lab of Professor Helen Cooper at the University of Birmingham and has a secondment at the Rosalind Franklin Institute (RFI). Across both institutions, his research focus is the development of native ambient mass spectrometry (NAMS) encompassing MS imaging of intact protein complexes, native top-down MS and instrument/method development. Oliver has presented at multiple international conferences and was a tutor at the American Society for Mass Spectrometry’s Fall Workshop on Native Mass Spectrometry.</blockquote></div></div></div>

Thermo Fisher Scientific: Imaging and top-down analysis of intact protein complexes in tissue

Imaging and top-down analysis of intact protein complexes in tissue

Join this webinar to hear about latest methods and advances for probing intact protein complexes in tissue using in situ native mass spectrometry.

Imaging and top-down analysis of intact protein in tissue

<h4>Key Learning Points</h4><ul><li>Understand how SLE and SPE work and how they are different from each other.</li><li>Evaluate the advantages and limitations of each method for various matrices.</li><li>Select the appropriate technique for analyte chemistry and target sensitivity.</li></ul><h4>Who Should Attend</h4><ul><li>LC or LC‑MS users evaluating or optimizing sample prep workflows.</li><li>Analysts working with challenging biological or environmental matrices.</li><li>Scientists who are unsure when to apply SLE vs. SPE for maximum recovery and cleanliness.</li><li>Method developers seeking to improve robustness, throughput, or reproducibility.</li></ul><blockquote>Speaker: Grace Guo &nbsp;(Senior Technical Specialist, Phenomenex)Grace Guo graduated from Johns Hopkins University with a Master’s degree in Material Science and Engineering. She joined Phenomenex Technical Team in 2017 providing technical support globally. With more than 9 years of experience in SPE, HPLC and GC, she delivered 90+ seminars and webinars globally covering food and environmental, clinical, pharma and biopharma industry.</blockquote>

Phenomenex: Extraction Face-Off: Choosing Between SLE and SPE

Extraction Face-Off: Choosing Between SLE and SPE

Understand how SLE and SPE work and how they are different from each other.

Choosing Between SLE and SPE

As Sustainable Aviation Fuel (SAF) production expands globally, fuel testing is becoming more demanding. New feedstock pathways and evolving processing routes introduce analytical complexity that can challenge traditional methods.Join us for an interactive session on how Combustion Ion Chromatography (C-IC) is transforming fuel testing performance in modern laboratories. We will also provide technical insights into the Thermo Scientific™ Cindion ™ C-IC system architecture and how it supports robust, reproducible results in demanding Oil &amp; Gas applications.<h5>In this webinar, you will learn:</h5><ul><li>The analytical challenges contract testing laboratory encounters with emerging fuel matrices for ASTM D7359, and D7566 methods</li><li>How Cindion C-IC System overcomes these challenges</li><li>The measurable improvements achieved in accuracy, compliance, efficiency, and reliability</li></ul><h5>Who should attend?</h5>This session is designed for professionals who are:<ul><li>Struggling with complex SAF matrices and inconsistent results</li><li>Facing increasing ASTM compliance and regulatory pressure</li><li>Looking to improve reproducibility and reduce rework</li><li>Seeking ways to streamline fuel testing workflows and boost efficiency</li></ul>Bring your questions! Jonathan from Saybolt and our C-IC Product Manager, Pavan, will address your technical and application inquiries directly in the live Q&amp;A session.<blockquote>Presenter: Jonathan Chan (Technical Chemist, Saybolt Singapore)Jonathan is a Technical Chemist at Saybolt Singapore with experience in petroleum, biofuels, and renewable fuel analysis. His work focuses on developing and validating analytical methods for complex fuel matrices, including trace elemental analysis using advanced techniques such as combustion ion chromatography and ICP-based methods. At Saybolt, Jonathan supports both routine laboratory operations and technical development projects, helping ensure reliable and defensible analytical results for the evolving energy landscape.Presenter: Pavan Sandhu (Product Manager, Thermo Fisher Scientific)Pavan is a Product Manager in the Ion Chromatography &amp; Sample preparation-DIA &nbsp;(IC/SP-DIA) business unit at Thermo Fisher Scientific managing Combustion IC &amp; Discrete Analyzer products.Pavan completed her MS in Biomedical Engineering from University of Texas at Dallas and has several years of experience in clinical diagnostics and analytical instrumentation. Prior to joining IC/SP-DIA, she was part of an R&amp;D team at Thermo Fisher’s Clinical Diagnostic Division (CDD) leading product development &amp; design transfer of multiple diagnostic products. In her current role, she is focussed on driving innovative ion chromatography solutions for our customers..</blockquote>

Thermo Fisher Scientific: Transforming Fuel Testing Performance with Combustion Ion Chromatography

Transforming Fuel Testing Performance with Combustion Ion Chromatography

Join us for an interactive session on how Combustion Ion Chromatography (C-IC) is transforming fuel testing performance in modern laboratories.

Fuel Testing with Combustion Ion Chromatography

This webinar explores how potential PFAS contamination can sporadically enter the analytical workflow from sample preparation through the LC chromatography system. Fluoropolymer‑based components and inadequately screened consumables frequently introduce background PFAS that can lead to false positives. While regulatory methods emphasize the importance of clean materials and rigorous blank testing to ensure PFAS‑free workflows, practical mitigation strategies can help identify potential contamination sources and outline best practices to prevent them. These strategies include using PFAS‑specialized products to support a comprehensive workflow contamination‑control approach.<h4>Key Learning Points</h4><ul><li>Identify and avoid potential sources for sporadic PFAS contamination in the PFAS workflow.</li><li>Learn how to apply best practices for sample preparation and analytical instrumentation.</li><li>Gain insight into products that support effective management of PFAS contamination.</li></ul><h4>Who Should Attend</h4><ul><li>Any analytical professionals who are beginning or expanding their work with PFAS by LC-MS/MS across both regulated and non-regulated methods and who are navigating background contamination, method sensitivity demands, and the practical challenges of troubleshooting persistent PFAS interferences.</li></ul><blockquote>Speaker: Maryam Sayeed (Product Manager, Phenomenex)Maryam Sayeed is a Product Manager at Phenomenex and has been with the company for three years. She focuses on expanding solutions for chromatography products and consumables designed for the food and environmental markets. Her work also includes supporting products that address challenges associated with PFAS analysis and background contamination at low detection limits. Prior to joining Phenomenex, Maryam spent nine years in the food and beverage industry, gaining extensive hands on experience with HPLC analysis. She holds an MS in Biology with a specialization in Biochemistry.</blockquote>

Phenomenex: Strategies to Mitigate Sporadic PFAS Contamination in Analytical Workflows

Strategies to Mitigate Sporadic PFAS Contamination in Analytical Workflows

Webinar on PFAS contamination in LC-MS workflows—learn sources, prevention strategies, and best practices for accurate low-level PFAS analysis.

Mitigate Sporadic PFAS Contamination in Analytical Workflows

Join this presentation and open discussion with industry-leading experts Patrick Muller and Kieran Evans – leaders in UV/Vis and IR analytical methods and testing techniques. This talk is designed for scientists in optics development and the high-end research of optical properties who work in academia, solar, space, defense and other high-performance applications.<h4>Key Learning Objectives:</h4><ul><li>Understand key measurement requirements for optical components and thin films, using both the UV/Vis/NIR and FTIR instrumentation.</li><li>Better understand material properties for real-life applications in industry, defense and the environment.</li><li>Learn about the importance of sampling flexibility in UV/Vis/NIR analysis.</li><li>See how specialist infrared equipment such as Optica can further advance your research insights including in-transmission measurements.</li></ul><h4>Who Should Attend:</h4>Scientists investigating optical properties who work in academia, solar, space, defense and other high-performance applications:<ul><li>Laboratory manager</li><li>Material Scientist</li><li>Research/Researcher</li><li>Optics Scientists</li><li>Principal Investigators</li><li>Lead Researcher</li></ul><blockquote>Speaker: Kieran Evans (Product Manager – UV, PerkinElmer UK)Speaker: Dr. Patrick Müller (Product Specialist FT-IR/FT-NIR, PerkinElmer)Speaker: Sara Kobilka (Contributing Editor, C&amp;EN Media Group)</blockquote>

C&EN: Precision Lab Solutions for High-End Optics and Coatings

Precision Lab Solutions for High-End Optics and Coatings

Join this presentation and open discussion with industry-leading experts in UV/Vis and IR analytical methods and testing techniques.

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