Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis">LC–MS/MS</a> quantifying a novel biologic’s metabolic fate, or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis">GC–MS/MS</a> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.<h3>Biopharma: The Engine Room of LC–MS/MS Demand</h3><h4>GLP-1 Agonists and the Metabolomics Gold Rush</h4>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163">semaglutide and related peptide therapeutics </a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22">Chromatography podcast</a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html">one in eight Americans are currently taking one of these drugs such as Ozempic</a>.<h4>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</h4>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">Nitrogen evaporation workflows</a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.<h4>Precision Medicine and Multi-Omics Integration</h4>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3">Orbitrap </a>and Q–TOF hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.<h3>Environmental Mandates: PFAS Testing Drives Adoption</h3>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.<h4>The Regulatory Framework Chemists Need to Know</h4>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe">EPA Method 533</a>: Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination">EPA Method 537.1</a>: Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg">EPA Method 8327</a>: LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633">EPA Draft Method 1633</a>: The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.<h4>The Sample Preparation Bottleneck</h4>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"> PFAS in laboratory materials</a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385">inert nitrogen evaporation </a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.<h3>Technological Trends Reshaping the LC–MS/MS Workflow</h3><h4>Artificial Intelligence and Automated Data Interpretation</h4>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u">Agilent’s acquisition of Virtual Control’s ACIES </a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.<h4>Online SPE Coupling and “Chromatography Without Walls”</h4>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392">nitrogen-assisted evaporation</a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407">liquid–liquid extraction</a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.<h4>High-Purity Solvents and Reference Materials</h4>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.<h3>The Ever-Present Need for Sample Evaporation</h3>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.Several specific developments are particularly relevant:<ul><li>ADC and gene therapy characterization workflows require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li>PFAS trace analysis demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li>High-throughput pharmaceutical QC driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li>Multi-omics and metabolomics workflows generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;

The Continued Rise of Tandem Chromatography–Mass Spectrometry

News from LabRulezLCMS Library - Week 27, 2026

🎤Dr. Christopher Reid (Professor of Chemistry and Biochemistry at Bryant University in Rhode Island)In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.<h4>What You’ll Learn in This Episode:</h4><ul><li>Why antimicrobial resistance is accelerating globally</li><li>What peptidoglycan is and why it’s essential to bacterial survival</li><li>How targeting cell wall degradation differs from traditional antibiotics</li><li>The strategy behind designing small molecule probes and inhibitors</li><li>How LC-MS is used to analyze peptidoglycan fragments and enzyme activity</li><li>Key analytical challenges: complex mixtures, low-abundance species, and structural similarity</li><li>The importance of sample preparation and reproducibility in LC-MS workflows</li><li>How this research could lead to new antibiotic targets</li></ul><h3>Video Transcription</h3><h4>The Growing Challenge of Antibiotic Resistance</h4>According to Reid, antibiotic resistance is increasing across a broad spectrum of bacterial pathogens that were once readily treated with existing therapies. In some cases, clinicians must now rely on only a handful of last-line antibiotics, while resistance is also rising in diseases such as tuberculosis.One of the major obstacles is the limited commercial incentive to develop entirely new antibiotics. Because bacterial resistance can emerge relatively quickly after a drug reaches the market, pharmaceutical companies often favor modifying existing antibiotics or combining them with resistance-modifying agents rather than investing in completely new drug classes. As a result, genuinely novel antibacterial targets remain urgently needed.<h4>Why the Bacterial Cell Wall Remains an Attractive Target</h4>The bacterial cell wall consists primarily of peptidoglycan, a highly cross-linked polymer that provides structural integrity, maintains cell shape, and enables bacterial growth and division.Many widely used antibiotics—including the β-lactam antibiotics such as penicillins—target enzymes responsible for synthesizing or cross-linking peptidoglycan. These pathways have proven highly successful therapeutic targets because peptidoglycan is unique to bacteria and absent from human cells, minimizing unwanted effects on the host.However, most existing antibiotics target only specific stages of peptidoglycan biosynthesis. Reid's research explores another part of this complex pathway: enzymes responsible for degrading the carbohydrate backbone of peptidoglycan during normal bacterial growth and remodeling. These enzymes may represent an underexplored class of antimicrobial targets capable of overcoming existing resistance mechanisms.<h4>Targeting the Glycan Backbone</h4>A key advantage of focusing on the carbohydrate backbone is its remarkable conservation across both Gram-positive and Gram-negative bacteria.Whereas the peptide components of peptidoglycan vary considerably between bacterial species—and frequently acquire mutations associated with antibiotic resistance—the glycan backbone remains highly conserved and tolerates relatively few structural modifications.This biological constraint makes enzymes acting on the glycan backbone particularly attractive targets. Reid's group developed a series of small-molecule probes and inhibitors designed to recognize enzymes known as N-acetylglucosaminidases, which cleave glycosidic bonds within peptidoglycan and generate soluble degradation products.Rather than beginning with purified enzymes, the researchers adopted a top-down phenotypic screening strategy. Candidate compounds were first evaluated for their ability to inhibit bacterial growth before progressively narrowing the list of potential molecular targets through genetic studies, microscopy, enzyme assays, and biochemical characterization. This iterative workflow ultimately identified compounds capable of inhibiting the desired enzyme class while simultaneously providing valuable chemical probes for studying bacterial cell wall biology.<h4>LC-MS as a Central Analytical Tool</h4>Liquid chromatography coupled with mass spectrometry plays a central role throughout the project by enabling detailed characterization of structural changes occurring within peptidoglycan after bacterial cells are treated with candidate inhibitors.Because intact peptidoglycan is an insoluble polymer, sample preparation begins with isolation of the bacterial cell wall through detergent extraction and differential centrifugation. Additional purification steps remove proteins, nucleic acids, and teichoic acids before enzymatic digestion converts the insoluble material into soluble fragments suitable for chromatographic analysis.The resulting digest is analyzed by LC-MS to examine changes in the distribution of peptidoglycan fragments. Chromatographic retention times provide an immediate indication of changes in cross-linking, with larger oligomers such as trimers and tetramers generally eluting later than monomeric fragments. Mass spectrometry then confirms the identity of these fragments by measuring their intact molecular masses, while tandem MS experiments can provide further structural information when required.<h4>Sample Preparation Determines Data Quality</h4>Reid emphasized that sample preparation represents one of the most critical—and challenging—stages of the entire workflow.Because peptidoglycan analysis involves multiple purification, digestion, and reduction steps, even minor procedural differences between samples can introduce analytical artifacts that complicate biological interpretation.Maintaining strict consistency throughout sample preparation is therefore essential. Appropriate controls must accompany every experiment, ensuring that observed chromatographic and mass spectrometric differences truly reflect biological responses rather than experimental variability. The complexity of bacterial cell wall composition further increases the analytical challenge, as subtle differences in amino acid substitutions and low-abundance cross-linked species can complicate LC-MS data interpretation.<h4>Chemical Biology for Future Antibiotic Discovery</h4>Although the identified compounds may eventually serve as starting points for antibiotic development, Reid views them primarily as chemical biology tools.By selectively inhibiting individual enzymes, these probes allow researchers to investigate previously unexplored aspects of bacterial cell wall remodeling that cannot easily be studied using classical genetic approaches alone.The research team is now expanding this strategy by combining their probes with proximity-labeling proteomics and mass spectrometry, aiming to map protein interaction networks associated with bacterial cell wall degradation. Such studies may reveal entirely new antibacterial targets and provide deeper insight into the biological processes governing bacterial growth and division.Ultimately, the work illustrates how integrating synthetic chemistry, microbiology, molecular biology, and advanced LC-MS can accelerate the search for innovative therapeutic strategies against antimicrobial resistance. As existing antibiotics become progressively less effective, multidisciplinary approaches such as these may play an increasingly important role in identifying the next generation of antibacterial targets.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

Article

Application

Academy

Video

Scientists

Interview

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Separation Science

Bruker

GERSTEL

Phenomenex

Thermo Fisher Scientific

SelectScience

SCIEX

Waters Corporation

Advances in metabolomics and lipidomics are generating unprecedented volumes of complex data, but confident identification and biological interpretation of small molecules remain significant challenges. Limitations in annotation, data dimensionality, and reproducibility can slow discovery and reduce confidence in downstream decision‑making across clinical, translational, and applied research.In this live broadcast event, leading experts explore how next‑generation mass spectrometry technologies, ion mobility, isotope‑based filtering, and computational tools are helping researchers move from complex datasets to confident insight. Through two short, focused scientific talks, speakers will share real‑world examples of how multi‑dimensional data and advanced workflows are being used to improve metabolite and lipid annotation, accelerate small‑molecule discovery, and enable more robust and reproducible analyses.Broadcast as a single, high‑impact session and presented in partnership with Bruker, this webinar provides practical and conceptual guidance for scientists looking to push the boundaries of metabolomics and lipidomics research using modern analytical platforms such as timsMetabo™.<h5>This broadcast is designed for:</h5><ul><li>Scientists using mass spectrometry in metabolomics, lipidomics, and small‑molecule analysis</li><li>Researchers in clinical, translational, biopharmaceutical, and toxicology settings</li><li>Teams working in biomarker discovery, exposomics, and pathway analysis</li><li>Lab leaders evaluating next‑generation MS and ion mobility platforms</li></ul><blockquote>Speaker: Dr. Michael Witting (Deputy Head Metabolomics and Proteomics Core &amp; Executive Manager Metabolomics, Helmholtz Munich)Speaker: Prof. Pieter Dorrestein (Skaggs School of Pharmacy and Pharmaceutical Sciences, Departments of Pharmacology and Pediatrics)Speaker: Pelle Simpson (Principal Scientist, Enveda)Moderator: Cameron Smith-Craig (Pharma and Applied Sciences Editor, SelectScience)</blockquote>

Bruker: Next‑generation metabolomics: From annotation to discovery at scale

Next‑generation metabolomics: From annotation to discovery at scale

Advance small-molecule discovery with Bruker's next-gen metabolomics. Learn how ion mobility and timsMetabo™ improve metabolite and lipid annotation

Next-Generation Metabolomics and Lipidomics Discovery

Modern biopharma pipelines are rapidly expanding beyond conventional monoclonal antibodies into multi‑specific formats and bioconjugates such as antibody–drug conjugates (ADCs) and antibody–oligonucleotide conjugates (AOCs). While these modalities unlock new therapeutic possibilities, their structural complexity significantly increases analytical demands. Molecular assembly, chain pairing, size, charge, and hydrophobic heterogeneity, post-translational modifications, and stability can all vary in subtle yet clinically meaningful ways. &nbsp;In this webinar, we present a practical, end‑to‑end characterization framework that connects LC-based strategies across structural levels—intact, subunit, and peptide—and shows how orthogonal separations support confident decisions from development through QC transfer. Topics include approaches to verify structural integrity, reveal mispaired or incorrectly assembled species, monitor size variants and aggregation, assign charge variants, and apply fit‑for‑purpose workflows to conjugated antibodies where additional heterogeneity must be controlled.Attendees will leave with a clear “analytical toolbox” perspective—understanding which techniques to apply, what questions each method answers, and how to assemble robust characterization strategies for emerging antibody modalities.<h4>What you'll learn</h4><ul><li>How multispecific formats and conjugates change analytical strategy vs. traditional mAbs</li><li>How LC separates and quantifies variants, while LC–MS enables molecular confirmation</li><li>How to align intact, subunit, and peptide workflows to specific analytical questions</li><li>How conjugation introduces new heterogeneity—and how to manage it effectively (ADC/AOC)</li></ul><h4>Why attend?</h4>As antibody therapeutics evolve from conventional mAbs to multi‑specific architectures and bioconjugates (ADCs and AOCs), analytical teams face complexity—more variants, more heterogeneity, and a greater need for orthogonal evidence to support confident development and control strategies.In this webinar, we share a pragmatic characterization roadmap using LC-based methods across intact, subunit, and peptide‑mapping workflows—showing how each layer answers different questions, and how to assemble them into a cohesive package.<h4>Key takeaways include:</h4><ul><li>Selecting LC/LC‑MS approaches for size, charge, and hydrophobic variants,</li><li>Detecting mispaired or incorrectly assembled multi‑specific species</li><li>Handling the added complexity of conjugated antibodies (ADC/AOC) within fit‑for‑purpose workflows</li></ul>Ideal for scientists in analytical development, CMC, and QC supporting next‑generation antibody therapeutics.<h4>Who should attend?</h4>Analytical scientists in biopharma R&amp;D, process development, and QC working with advanced antibody modalities.You can select the language of the content in the top right corner of your screen. This webinar will be delivered in English.<blockquote>Speaker: Ali Tiss, Ph.D., MSc., Eng. (Chat) (Chemistry Market Development Manager, Biopharma &amp; Cell and Gene Therapies, Waters Corporation)Ali joined Waters in 2021 and has since supported colleagues and users in the biomolecule separation field (proteins, peptides, Abs, conjugates, Cell &amp; Gene Therapy products). Ali completed his Ph.D. in biochemistry and structural biology at the Mediterranean University (CNRS, Marseille, France), where he contributed to the R&amp;D of the anti-obesity drug, Xenical®, developed by Roche Ltd. Then, he earned over two decades of research experience in biochemistry, liquid chromatography, mass spectrometry, and proteomics, focusing on detecting and characterizing biomarkers related to human diseases such as cancer, diabetes, and obesity. Ali is passionate about supporting customer business and innovative applications.</blockquote>

Characterizing Next-Generation Antibody Therapeutics: LC Strategies for Multispecifics, ADCs & AOCs

In this webinar, we share a pragmatic characterization roadmap using LC-based methods across intact, subunit, and peptide‑mapping workflows

LC Strategies for Multispecifics, ADCs & AOCs

The presence of antibiotics in aquatic environments is a growing analytical challenge, with trace-level contamination posing significant risks to ecosystems and contributing to antimicrobial resistance. Accurate, reliable and high-throughput sample preparation is therefore essential for effective water quality monitoring.This webinar will demonstrate the use of the Biotage® PrepXpert-8 automated solid-phase extraction (SPE) system for the simultaneous extraction and enrichment of multiple antibiotic classes, including fluoroquinolones, tetracyclines, sulfonamides and macrolides from water matrices.We will cover method development considerations, sorbent selection and the advantages of full automation in reducing operator variability and improving reproducibility. Quantitative data and method performance metrics will be presented and discussed.Whether you are new to SPE or looking to streamline existing workflows, this webinar offers practical insights for modern environmental water analysis.By attending this presentation, you will:<ul><li>Gain practical knowledge of automated SPE method development for extracting multiple antibiotic classes from water matrices using the Biotage® PrepXpert-8.</li><li>Learn key sorbent selection strategies and see real quantitative recovery data to support your own method implementation.</li><li>Leave equipped to streamline your sample preparation workflows and improve reproducibility in water analysis.</li></ul><h5>Who should attend?</h5>This webinar is ideal for analytical and environmental chemists, water quality scientists and laboratory managers working in environmental monitoring, regulatory compliance, or pharmaceutical research who are involved in trace-level contaminant analysis.<h5>What you need to know:</h5>Start times:<ul><li>Broadcast 1: 8 am BST (London/Lisbon) / 9 am CEST (Paris/Berlin) / 12:30 pm IST (New Delhi) / 3 pm CST (Beijing) / 4 pm JST (Tokyo)</li><li>Broadcast 2: 8 am CDT (Chicago) / 9 am EDT (New York) / 2 pm BST (London/Lisbon) / 3 pm CEST (Paris/Berlin)</li><li>Broadcast 3: 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)</li></ul>Duration: 1 hour<blockquote>Presenter: Vainius Skukauskas (Applications Chemist, Biotage EMEA Application Laboratory, Cardiff, UK)Dr. Vainius Skukauskas is an Applications Chemist at the Biotage EMEA Application Laboratory in Cardiff, UK. With a Ph.D. in chemistry and extensive expertise in analytical method development, he specializes in developing and optimizing automated sample preparation workflows across a wide range of industries, with a particular focus on the environmental and food sectors. He works closely with customers to translate complex methods into robust, high-throughput solutions, and regularly delivers technical demonstrations, training, and workshops across EMEA.</blockquote>

Separation Science: Advances in Antibiotic Extraction from Water - An Automated SPE Approach

Advances in Antibiotic Extraction from Water: An Automated SPE Approach

Advance water quality monitoring. Discover automated SPE method development and sorbent selection for trace-level antibiotic extraction with Biotage

Automated SPE Approach for Antibiotic Extraction from Water

Join us for an insightful webinar on the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in food substances using advanced LC-MS/MS technology. This session will cover the importance of PFAS testing in food safety, key analytical challenges, sample preparation strategies, method development approaches, and best practices for accurate and sensitive detection using LC-MS/MS systems. Experts will also discuss workflow optimization, regulatory considerations, and real-world applications across different food matrices, helping laboratories and researchers strengthen their PFAS analysis capabilities.<h5>Key highlights:</h5><ul><li>PFAS in food and it’s regulation around the world</li><li>Analytical challenges &amp; SCIEX LC-MS/MS solutions</li><li>QTRAP workflow to confirm the true or false results in food samples &nbsp;</li></ul><blockquote>Expert: Kankan Jana (Application Support Specialist, SCIEX)</blockquote>

SCIEX: Analysis of per- and polyfluoroalkyl substances (PFAS) in food substances using LC-MS/MS solutions

Analysis of per- and polyfluoroalkyl substances (PFAS) in food substances using LC-MS/MS solutions

Advance food safety testing. Discover SCIEX LC-MS/MS methods and QTRAP workflows for accurate detection and regulatory compliance of PFAS in food

PFAS Analysis in Food Safety: SCIEX LC-MS/MS Solutions

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Leverage unique capabilities and powerful tools to streamline sample analysis. Are you using your Thermo Scientific Vanquish UHPLC systems to their fullest potential?Join this webinar to discover the multiple tools in Thermo Scientific Chromeleon software that drive sample throughput from acquisition to data processing and deliver reduced errors through advanced automation.<h4>Why attend</h4>Uncover the Chromeleon software features that drive sample throughput and deliver unique capabilities in combination with the Thermo Scientific Vanquish Duo UHPLC system. In this webinar, you will see how Chromeleon software can help:<ul><li>Increase sample throughput through streamlined workflows</li><li>Leverage the full capabilities of the Vanquish Duo UHPLC system&nbsp;</li><li>Deliver automation to common analyses to reduce errors</li></ul>Through practical examples and live software demonstrations, attendees will learn how to improve day-to-day laboratory efficiency and reduce errors.<h4>What you will learn</h4>By attending, participants will be able to:<ul><li>Streamline analysis in Chromeleon software from acquisition to result</li><li>Understand and leverage the unique capabilities of Vanquish Duo UHPLC systems &nbsp;</li><li>Drive automation in common workflows to reduce errors</li></ul>Save your spot to discover practical ways to optimize Vanquish UHPLC system workflows with Chromeleon software.Cannot attend on July 9? Please register anyway to automatically receive the link to the recording.<blockquote>Presenter: Darren Barrington-Light (Senior Manager, Product Marketing, Thermo Fisher Scientific)Darren Barrington-Light’s background includes over 25 years of experience in the field of analytical chemistry, chromatography hardware and computer software. Having worked in chromatography throughout his career, Darren has a unique understanding of the requirements of organizations that rely on chromatography data to manage their day to day operations. As capabilities have advanced, he has worked with clients across multiple industries to show them how software solutions can help them to improve their day to day operations and drive fast, informed decisions.</blockquote></div></div></div>

Thermo Fisher Scientific: Advanced tools to maximize your sample throughput with Chromeleon Software and Vanquish UHPLC

Advanced tools to maximize your sample throughput with Chromeleon Software and Vanquish UHPLC

Discover how Chromeleon software optimizes Vanquish UHPLC workflows with automation, higher throughput, and reduced errors from acquisition to results.

Sample throughput with Chromeleon and Vanquish UHPLC

<h4>A Practical Webinar for Wastewater Testing and Compliance Professionals</h4>Nitrogen analysis plays a critical role in wastewater treatment, compliance monitoring, and nutrient management, yet selecting the right method and interpreting results correctly can be complex. In this webinar, we’ll break down the most common nitrogen parameters, including ammonia, nitrate/nitrite, TKN, and total nitrogen, and explain how these measurements support both regulatory reporting and process control.Attendees will gain practical insight into commonly used analytical methods, sample handling and QA/QC best practices, and how to avoid common reporting and interpretation mistakes. We’ll also review real-world scenarios involving nitrification, denitrification, permit compliance, and troubleshooting elevated nitrogen results.Whether you work in a laboratory, wastewater treatment facility, or environmental compliance role, this session will provide the clarity and confidence needed to improve data quality, optimize operations, and make informed nitrogen testing decisions.<h4>Key takeaways</h4><ul><li>Understand key nitrogen methods and reporting requirements</li><li>Improve testing accuracy with better QA/QC and sample handling</li><li>Gain practical insights for compliance, troubleshooting, and process optimization</li></ul><h4>Who should attend</h4>This webinar is designed for you if you are:<ul><li>Analytical Chemist</li><li>QA Manager</li><li>Process Engineer</li><li>Wastewater Operator</li></ul><blockquote>Speaker: Sam Johnson (Inorganic Chemist, Waters ERA)Sam has been with Waters ERA for 11 years, specializing in the manufacturing and analytical verification of inorganic standards and reagents.He holds a Bachelor of Science in Geology with a minor in Watershed Science from Colorado State University in Fort Collins.Throughout his career, Sam has supported the environmental testing industry by providing solutions for wastewater, drinking water, and soil testing laboratories.Speaker: Christy Abbas, PhD (Sr. Product Marketing Manager, Waters ERA)Christy has been with Waters ERA for six and a half years. Her career spans roles at an instrument manufacturer, a biopharmaceutical laboratory, and a mining company. She earned her PhD in Applied Chemistry from the Colorado School of Mines.Throughout her career, Christy has supported the nuclear, pharmaceutical, and chemical industries, as well as academia. Drawing on her background in analytical chemistry, product development, marketing, and sales, she is focused on expanding Waters ERA’s product portfolio to better meet evolving customer needs.</blockquote>

Cracking the Code on Nitrogen Methods

Discover practical approaches to nitrogen analysis in wastewater, including testing methods, QA/QC, compliance requirements, and process optimization.

Cracking Code on Nitrogen Methods

Advances in metabolomics and lipidomics are generating unprecedented volumes of complex data, but confident identification and biological interpretation of small molecules remain significant challenges. Limitations in annotation, data dimensionality, and reproducibility can slow discovery and reduce confidence in downstream decision making across clinical, translational, and applied research.In this live broadcast event, leading experts explore how next generation mass spectrometry technologies, ion mobility, and computational tools are helping researchers move from complex datasets to confident insight. Through three short, focused scientific talks, speakers will share real world examples of how multi dimensional data and advanced workflows are being used to improve metabolite and lipid annotation, accelerate small molecule discovery, and enable more robust and reproducible analyses.<h4>Who Should Attend</h4><ul><li>Untargeted metabolomics and natural products researchers focused on spectral annotation and structure elucidation</li><li>Microbiome, exposomics, and environmental health scientists&nbsp;</li><li>Drug discovery scientists using AI/ML and computational chemistry</li><li>Core facility managers and method developers building reproducible, multi-dimensional LC-MS/MS workflows</li><li>Cheminformatics and computational scientists working with spectral repositories and ML-based metabolite prediction</li><li>Lab leaders evaluating next-generation ion mobility MS platforms for 4D-metabolomics and 4D-lipidomics</li></ul><h4>Key Learning Objectives</h4><ul><li>How to boost identification confidence and speed using timsMetabo™, isotope‑based filtering, and MassQL</li><li>Applying multi‑dimensional data (including ion mobility and CCS) to improve metabolite annotation</li><li>Tips for accelerating small‑molecule discovery and lipidomics workflows with robust, reproducible approaches</li><li>Navigating current bottlenecks in non‑targeted and targeted metabolomics and lipidomics</li></ul><blockquote>Speaker: PD Dr. Michael Witting, Deputy Head Metabolomics and Proteomics Core &amp; Executive Manager Metabolomics, Helmholtz Munich, Munich, GermanySpeaker: Pieter Dorrestein, Professor, Skaggs School of Pharmacy and Pharmaceutical Sciences Departments of Pharmacology and Pediatrics, UC San Diego, San Diego, CA, USASpeaker: Pelle Simpson, Principal Scientist, Enveda, Boulder, CO, USA</blockquote>

Bruker: 4D-Metabolomics™ in Action: Real‑World Strategies for Deep Discovery and Confident Annotation

4D-Metabolomics™ in Action: Real‑World Strategies for Deep Discovery and Confident Annotation

Next generation mass spectrometry technologies, ion mobility, and computational tools are helping researchers move from complex datasets to confident insight. 

4D-Metabolomics™ in Action

From high-throughput extraction and anti-clogging strategies to up-concentration and LC-MS/MS analysis.PFAS analysis in aqueous matrices requires robust sample preparation, reliable clean-up, and consistent recoveries - especially as ultra-short-chain PFAS such as TFA are gaining increasing regulatory attention.In this Velaris webinar, experts from PromoChrom and LCTech will present automated SPE-based workflows for PFAS analysis in drinking water, environmental water, wastewater, and other aqueous matrices compliance with methods such as EPA 533, EPA 537.1, EPA 1633, and EU Method 17892.The session will cover fully automated SPE with the PromoChrom SPE-03 platform, anti-clogging solutions for challenging samples, optimized PFAS SPE cartridges, and Velaris solutions for the extraction of ultra-short-chain PFAS.The webinar will also introduce the D-EVA vacuum concentrator for unattended sample concentration and show how automated sample preparation can support high throughput, reproducibility, low background levels.<blockquote>Presenter: Keith Keegan (Lead Applications Engineer, PromoChrom)Keith Keegan will focus on the PromoChrom SPE-03 platform for fully automated SPE, workflow efficiency, anti-clogging strategies, real-world laboratory use, and future-oriented tools such as real-time monitoring and remote fleet management.</blockquote><blockquote>Presenter: Dr. Suman Kharel (Application Specialist, LCTech)Suman Kharel will present optimized SPE cartridges for PFAS analysis, Velaris solutions for ultra-short-chain PFAS, performance data for aqueous matrices, and the use of D-EVA for unattended concentration before LC-MS/MS analysis.</blockquote>

GERSTEL: Automated SPE Sample Preparation for PFAS and Ultra-Short-Chain PFAS in Aqueous Matrices

Automated SPE Sample Preparation for PFAS and Ultra-Short-Chain PFAS in Aqueous Matrices

Master automated PFAS sample prep. Learn SPE-03 extraction techniques and D-EVA concentration for ultra-short-chain PFAS in compliance with EPA 1633

Automated SPE for Ultra-Short-Chain PFAS in Water

<h4>Key Learning Points</h4><ul><li>Understand how backpressure is measured in LC instruments.</li><li>Learn why backpressure is important to measure in the system and column.</li><li>Explore how different dimensions of column hardware and system tubing can influence overall backpressure.</li><li>Examine how different mobile phases and flow rates can impact backpressures.</li><li>Find out what different types of sample matrices can do to backpressure and how to mitigate.</li><li>Listen in to tips to troubleshoot the root cause of backpressure issues.</li></ul><h4>Who Should Attend</h4><ul><li>Any liquid chromatography users who are looking to understand the factors that can impact backpressure and how to mitigate potential backpressure problems.</li></ul><blockquote>Speaker: Koen Askamp (Senior Technical Specialist, Phenomenex)&nbsp;Koen Askamp is a Senior Technical Specialist in Phenomenex. He was awarded his BSc and MSc degrees in Molecular Life Sciences at Wageningen University in the Netherlands and joined Phenomenex in 2020 as Technical Sales Consultant. Koen became part of Phenomenex’s global technical support team in 2022 and assists laboratories in training chromatographers and developing analytical methods.&nbsp;</blockquote>

Phenomenex: Under Pressure - Mastering Backpressure in HPLC

Under Pressure: Mastering Backpressure in HPLC

Learn to manage HPLC backpressure. Discover how mobile phases, column hardware, and sample matrices impact system pressure with Phenomenex experts

Mastering HPLC Backpressure: Troubleshooting & Tips

As regulatory expectations around per- and polyfluoroalkyl substances (PFAS) continue to evolve, pharmaceutical laboratories are under increasing pressure to detect and quantify PFAS at ultra-trace levels across complex sample matrices.Join this live webinar to explore analytical approaches for sensitive, selective, and reproducible PFAS analysis in pharmaceutical applications.Drawing on representative workflows and quantitative performance data, this session will highlight strategies for PFAS monitoring in active pharmaceutical ingredients (APIs) and pharmaceutical packaging materials using the SCIEX 7500+ system.Attendees will gain insight into method development considerations, contamination control strategies, chromatographic separation approaches, and quantitative performance for broad PFAS panels in pharmaceutical workflows.<h4>What you will learn</h4><ul><li>Key analytical challenges associated with PFAS monitoring in pharmaceutical workflows, including contamination control and complex sample matrices</li><li>Approaches for sensitive and selective PFAS quantitation in APIs and pharmaceutical packaging materials using LC-MS/MS</li><li>Chromatographic and workflow strategies to improve PFAS separation, reproducibility, and quantitative confidence</li><li>Quantitative performance results, including LOQs as low as 0.005 ng/mL, broad linear dynamic range, and recovery performance across multi-compound PFAS panels</li></ul><blockquote>Speaker: Dr. Cathy LaneJoined SCIEX in 2006 as a Support Specialist and in 2021 took up the role of EMEAI Applications Lead for Pharma &amp; Peptide Quant.She has more than 20 years’ experience in the development of LC/MS methods across multiple application areas, and specializes in workflows for peptide quantification.She obtained her PhD in mass spectrometry-based proteomics from the University of London School of Pharmacy in 2004</blockquote>

PFAS in pharmaceuticals

Discover analytical strategies for sensitive PFAS monitoring in APIs and pharmaceutical packaging using LC-MS/MS and advanced contamination control

Related content

Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

The Continued Rise of Tandem Chromatography–Mass Spectrometry

News from LabRulezLCMS Library - Week 27, 2026