<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=136&amp;manufacturerItems=50&amp;page=2">Solid phase extraction</a> is routinely selected as a sample preparation technique prior to HPLC and GC analysis. It provides:<ul><li>Clean-up;</li><li>Analyte pre-concentration;</li><li>Solvent switching.</li></ul>This works well for any homogeneous liquid samples, but clearly a solid sample cannot be applied directly to an SPE device. Here we will consider how best to approach the handling of solid samples prior to SPE.The first stage of sample pre-treatment involves homogenising the solid sample with a suitable solvent. The solvent selected for this purpose will:<ul><li>Be effective at solubilising the analyte.</li><li>Be effective at solubilising the sample matrix, although this is not necessary providing the sample is blended well with the solvent.</li><li>Ideally it will be a weak solvent when it comes to the SPE stage. This may not be possible, and it may be necessary to dry down and reconstitute the sample prior to loading on to the SPE cartridge.</li></ul>Having formed a suspension of the sample in a suitable solvent the sample should be vortexed to promote thorough mixing, and then centrifuged to pellet the insoluble components of the matrix. The solvent should then be removed for further treatment.At this point the SPE mechanism needs to be selected:&nbsp;<h3>Reversed Phase SPE Method</h3>Mechanism / Weak Solvent / Strong Solvent<ul><li>Reversed phase – Water/buffer – Acetonitrile/Methanol (hexane strongest)</li><li>Normal phase – Hexane/Heptane – Mid polar organic such as DCM, MTBE, IPA (strongest is water)</li><li>Ion exchange – Aqueous buffer at a pH which promotes ion exchange interactions between analyte and SPE sorbent – Organic solvent, typically methanol with pH adjustment to disrupt the ion exchange interactions.</li></ul>Analyte properties and sample diluent both need to be considered at this point. Reversed phase extractions are effective for moderately polar to moderately hydrophobic analytes, but only if they are dissolved in a weak solvent such as water. If the sample is dissolved in a mid-polar solvent the sample will either need to be diluted, or alternatively dried down and reconstituted. The same is true for normal phase, where samples need to be dissolved in hexane/heptane for analytes to be successfully retained during sample loading onto the SPE device.These examples will hopefully illustrate this concept.<h4>The extraction of PCBs from soil.</h4>PCBs are hydrophobic and will dissolve readily in a non-polar solvent. Soil can be dried, weighed and then extracted using hexane (or heptane). Once thoroughly mixed and centrifuged the resulting solution can be applied directed to a conditioned silica SPE tube allowing for a normal phase extraction to be conducted.<h4>Tylosin from meat.</h4>Tylosin is an antibiotic, with a log P of 1.0. Extracting from meat with a mixture of acetonitrile and water (80:20) will break any hydrophobic interactions releasing the tylosin. After centrifugation the resulting liquid sample will require further dilution with water to reduce the loading solvent strength prior to a reversed phase extraction.<h4>Enilconazole from oranges.</h4>Enilconazole is a fungicide widely used in agriculture and is often used with citrus fruits. It can readily be extracted from oranges using acetonitrile. As enilconazole is basic, the addition of HCl to the acetonitrile extract will promote protonation of the analyte, allowing for it to be directly retained on a strong cation exchange SPE material. This greatly simplifies the extraction process as no dilution of the sample is required (in contrast to reversed phase, or normal phase extraction where 100% acetonitrile would cause the analyte to be unretained during loading).

Considerations when working with solid samples and SPE

ALS recognizes that Climate Change is one of the defining issues of our time and we strive to make Impact through Action in the areas of Sustainability, linked to the ALS core value of Caring.ALS intends to make the world a better place through innovation, science, and commitment to best practice. This year the ALS Board has signed off on a company commitment to Net Zero by 2050 as summarized on our <a target="_blank" rel="noopener noreferrer" href="https://www.alsglobal.com/en/sustainability?utm_source=email-sig-link-ca&amp;utm_medium=email&amp;utm_campaign=sustainability-report-23">annual sustainability report</a>. ALS is pleased to share our milestones and targets with clients.<h3>Background</h3>In 2021, ALS developed a climate change strategy, setting a target of 40% reduction in scope 1 &amp; 2 emissions by 2030. By end of FY23 (March 2023), ALS had achieved a 58% reduction, and achieved carbon neutrality for scope 1 &amp; 2 emissions – well ahead of plan. In the same month, ALS recorded a key milestone with the ALS Board approving our roadmap to reach Net Zero by 2050 (shared at right), with milestones noted and completed actions shaded green.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_b157904cab.jpg" alt="ALS Czech Republic: EnviroMail™12/Europe: ALS Net Zero Commitments &amp; Roadmap in Support of our Staff, Clients, Communities &amp; Shareholders." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_b157904cab.jpg 112w," sizes="100vw" width="805" height="1120"><h3>Further detail</h3>Key projects to date have included purchasing Renewable Electricity (RE) from the grid (we utilize greater than 90% RE across all operations), installing &gt;1.8MW of solar photovoltaic (PV) systems across 16 site locations, and continued implementation of our ALS Green Building Standards, which focus on HVAC systems, building insulation, LED lights, energy efficient plant and equipment, moving to electric vehicles (EVs), and staff engagement on adopting climate friendly practices to reduce actual energy demand.This ALS Net Zero commitment will involve reducing absolute scope 1 and 2 emissions by 95% and scope 3 emissions by 90% by 2050, against a 2020 baseline year. This is a challenging task for laboratory operations given requirements for chemical, plastic, and glassware usage, and significant air heating and cooling requirements due to fume hoods which cause frequent cycling of laboratory air.The ALS Roadmap includes a near-term target to reduce absolute scope 1 and 2 greenhouse gas emissions by 78% by 2030 against a 2020 baseline year.Over the next 2 years ALS will measure our scope 3 greenhouse gas inventory to confirm the calculated scope 3 estimate completed in FY2023 and will develop action plans for freight, purchased goods/services, wastes, and employee commutes. A near-term target for 2030 for scope 3 will then be set.Priority actions over the next 5 years will include:<ul><li>purchasing renewable electricity for &gt;95% of our operations</li><li>installing further solar energy PV systems (7 more installed YTD for FY24 with a further 9 systems to be completed in early FY25)</li><li>converting gas ovens and furnaces to electric where possible</li><li>selection of more energy-efficient vehicles; 30% of fleet to be hybrids and EVs by 2030</li><li>upgrading to ALS green building guidelines for new laboratory developments and refurbishments (better insulation, more efficient HVAC, solar PV systems, and upgraded building management systems)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_2_91b5e7ebf8.jpg" alt="ALS Czech Republic: EnviroMail™12/Europe: ALS Net Zero Commitments &amp; Roadmap in Support of our Staff, Clients, Communities &amp; Shareholders." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_2_91b5e7ebf8.jpg 156w," sizes="100vw" width="1089" height="1089"><blockquote>We are pleased to share our commitments with our stakeholders. Please feel free to contact your local ALS representative for further information.</blockquote>

EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders

Solid-phase extraction (SPE) is one of the key techniques in modern analytical chemistry for pre-concentrating analytes prior to chromatographic analysis. However, traditional manual SPE procedures are often time-consuming, error-prone, and difficult to reproduce. In response to laboratories’ increasing demand for automation and efficiency, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Watrex Praha</a> introduces the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/streamline-ole-200/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Streamline OLE 200</a> – an advanced system for online SPE.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Ole_uvod_oprava_bd77e4d8c2.jpg" alt="Watrex: Streamline™ OLE 200" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Ole_uvod_oprava_bd77e4d8c2.jpg 245w," sizes="100vw" width="1599" height="900"><h4>Comprehensive Automation of SPE&nbsp;</h4>The <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/streamline-ole-200/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Streamline OLE 200</a> is a fully automated solid-phase extraction unit designed for direct integration into HPLC systems. It enables efficient pre-concentration of up to eight different samples, minimizes the need for manual handling, and significantly boosts laboratory productivity.<h4>Advantages of SPE Automation with OLE 200&nbsp;</h4><ul><li>Increased laboratory throughput: Automatic processing of up to 8 samples without the need for operator intervention.&nbsp;</li><li>Reduced manual errors and improved result reproducibility.&nbsp;</li><li>Flexible integration: Compatible with any HPLC system, allowing implementation across various laboratory settings.&nbsp;</li><li>Time savings: Eliminates the need to manually transfer samples between the SPE and HPLC steps.&nbsp;</li><li>Multimodal selection of SPE cartridges based on analytes and matrix complexity.&nbsp;</li></ul><h4>Technical Specifications and System Design&nbsp;</h4>At the heart of the system is a high-pressure pump, ensuring precise dosing of both samples and solvents. The system’s design features three key valves:&nbsp;<ul><li>A 10-port selection valve for sample and wash solvent selection.&nbsp;</li><li>A high-pressure SPE cartridge selector, allowing switching between up to five different SPE cartridges.&nbsp;</li><li>A standard injection valve, connecting the device to any HPLC system.</li></ul>This setup ensures that each sample is precisely processed: drawn into an SPE cartridge, washed with one or two eluents, and then eluted directly into the chromatograph.&nbsp;<h4>Functional Diagram&nbsp;</h4><ol><li>The sample is drawn by the pump through the 10-port valve to the selected SPE cartridge.&nbsp;</li><li>The cartridge is washed with one or two eluents to remove interferences.&nbsp;</li><li>Analytes are then eluted with a selected solvent and delivered directly into the HPLC system via the injection valve.&nbsp;</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_OLE_clanek_obr_2_36588f90b9.jpg" alt="Watrex: Fig. 2 - Streamline™ OLE 200 Schematic" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_OLE_clanek_obr_2_36588f90b9.jpg 245w," sizes="100vw" width="1700" height="957"><h4>Efficiency, Reproducibility, and Flexibility&nbsp;</h4>Automation of the entire SPE process leads to greater reproducibility and reduces the risk of contamination or human error. The system allows sequential processing of multiple samples, each of which can be analyzed following a specific protocol. Thanks to its open design, the OLE 200 is compatible with any HPLC system, making it easy to integrate into existing lab workflows without major modifications.&nbsp;<h4>Application Potential&nbsp;</h4>The OLE 200 system is particularly suitable for:&nbsp;<ul><li>Pesticide and pharmaceutical residue analysis in water&nbsp;</li><li>Clinical and pharmaceutical analysis of biological fluids&nbsp;</li><li>Food safety and environmental monitoring&nbsp;</li><li>Forensic and toxicological analyses&nbsp;</li></ul>Thanks to its modular design and the ability to select various SPE cartridges based on polarity and interactions, the device can be easily adapted to specific applications.&nbsp;<h4>Operation and Software&nbsp;</h4>The system's user-friendly interface enables easy setup of each SPE step, including cartridge selection, volumes, elution solvents, and wash sequences. The entire process can be fully programmed, minimizing the need for operator involvement during analysis.&nbsp;<h4>Conclusion&nbsp;</h4>The <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/streamline-ole-200/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Streamline OLE 200</a> from <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Watrex Praha</a> prepresents a modern and powerful tool for automated sample pre-concentration that significantly enhances efficiency, reliability, and accuracy in analytical laboratories. Its flexibility, robust design, and easy integration with existing systems make it an ideal solution for demanding applications in environmental analysis, pharmaceutical research, and routine quality control.&nbsp;<blockquote>Want to learn more? Visit our website at <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=linkedin&amp;utm_medium=propagace&amp;utm_campaign=linkedin_clanky">www.watrex.com</a> or email us at watrex@watrex.com.</blockquote>

Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC? 

Article

Product

Academy

Video

Environment

For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

ALS Czech Republic

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

BÜCHI

Agilent Technologies

Shimadzu Corporation

Waters Corporation

Supercritical Fluid Chromatography (SFC) was first developed in the 1960s, using supercritical carbon dioxide and this technology has been commercially available from Agilent for over a decade. Often known for its application to the analysis of chiral compounds, SFC is the ‘greener’ alternative over normal phase separations, further increasing the demand and acceptance of this technology.
This webinar will explain basic scientific principles of SFC as a highly powerful liquid separation technique for the efficient separation a wide range of analytes. Examples will be given to illustrate, how an SFC system can be coupled to a mass detector to form an integrated LC/MS system to deliver high throughput applications. 
<h3>What you will learn</h3>
<ul>
<li>Similarities and differences between SFC and conventional HPLC</li>
<li>Why SFC is the green technique in separation sciences.</li>
<li>The advantages of SFC with application examples</li>
<li>Theoretical background to SFC</li>
<li>How to set up an SFC system with MS detection</li>
</ul>
<blockquote>
Presenter: Dr. Jens Trafkowski (Product Specialist HPLC, Agilent Technologies)
</blockquote>
<blockquote>
Jens Trafkowski studied food chemistry and received his PhD at the Institute of Legal Medicine from the University of Bonn for his work on applications of HPLC-MS/MS in forensic and clinical toxicology.
</blockquote>
<blockquote>
Prior to joining Agilent in 2011, Jens spent more than 6 years as LC-MS an application specialist in industry before leaving to undertake a global HPLC product management responsibility at Agilent Technologies. Later, in 2017 Jens moved into his current role as a HPLC product specialist where he is based in Basel Switzerland.
</blockquote>

Agilent Technologies: SFC – only the solvent is critical

SFC – only the solvent is critical

This webinar will explain basic scientific principles of SFC as a highly powerful liquid separation technique for the  efficient separation.

Supercritical Fluid Chromatography (SFC) offers several benefits over High-Performance Liquid Chromatography (HPLC) for compound isolation due to the nature of supercritical fluids.
During the webinar find out:
<ul>
<li>What Supercritical Fluids are, and how they are used for compound isolation</li>
<li>How SFC takes advantage of HPLC</li>
<li>Why SFC instruments are the green standard for compound isolation</li>
<li>When SFC can speed up a process</li>
</ul>
<blockquote>
Presenter: Holger Gumm (Managing Director, Sepiatec GmbH)
</blockquote>
<blockquote>
I’m the founder and Managing Director at Sepiatec, a new product line of compact and easy-to-use SFC instruments recently joined to BUCHI’s established portfolio.
</blockquote>
<blockquote>
Presenter: Birke Götz (Product Manager Purification, BUCHI Labortechnik)
</blockquote>
<blockquote>
I am a Global Product Manager for Purification at BUCHI Labortechnik AG and located in the headquarter in Flawil, Switzerland. 
</blockquote>

BUCHI: Why SFC? An in-depth look into the benefits of this separation method

Why SFC? An in-depth look into the benefits of this separation method

SFC offers several benefits over HPLC for compound isolation due to the nature of supercritical fluids.

Why SFC? An in-depth look into the benefits

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 2 | Basics of Supercritical Fluid Chromatography Purification</h3>
Supercritical Fluid Chromatography (SFC) is an effective and green technique to isolate a compound(s) of interest from a mixture. SFC utilizes liquid CO2 to quickly isolate compounds while minimizing the efforts required to dry the collected fraction. In this module, we will discuss factors involved in SFC purification. 
Learning Objectives: 
<ul>
<li>Understand how to use SFC purification</li>
<li>Make informed decisions on method optimization</li>
<li>Understand impact of decisions made during purification on larger workflow</li>
</ul>
<blockquote>
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
</blockquote>

Waters Corporation: Basics of Supercritical Fluid Chromatography Purification

Basics of Supercritical Fluid Chromatography Purification

We will discuss factors involved in SFC purification.

Experience faster, more efficient, and higher quality purification processes with Shimadzu&#39;s Preparative LC and SFC Solutions.
It’s time to streamline your workflow with Shimadzu&#39;s leading-edge Preparative LC and SFC solutions. Our Nexera Prep products offer complete solutions that produce clean and reproducible isolates, with a user-friendly workflow that accommodates all skill levels. Here&#39;s how Shimadzu&#39;s Nexera Prep can help you save time and boost productivity:
<ul>
<li>Nexera Prep Standard – Fully equipped with advanced functions that ensure reliable purification of target components.
</li>
<li>Nexera MDAP – Capable of high purity preparative work with no target components left behind.
</li>
<li>Nexera UFPLC – Equipped with trap technology for concentration, purification, and collection in 100% organic solvent, reducing overall processing time.
</li>
<li>ASAPrep – Automatic scale-up from analytical to preparative scale, saving time and resources needed for optimization.
</li>
</ul>
Join us in the live webinar to learn how Shimadzu&#39;s Nexera Prep can streamline your workflow and produce outstanding results with minimal effort and time. Don&#39;t miss out on this opportunity – save your seat now!
Learning Objectives:
<ul>
<li>Understand how purification workflow can be streamlined for higher efficiency
</li>
<li>Explore Shimadzu’s leading-edge Preparative LC and SFC solutions
</li>
<li>Learn how automation plays a role in eliminating tedious and manual work
</li>
</ul>
<blockquote>
Presenter: Kosuke Nakajima (Innovation Centre Manager, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Kosuke Nakajima has strong technical knowledge in separation science, particularly LC, LC/MS, and Preparative LC system. Before joining Shimadzu Asia Pacific as an Innovation Centre Manager, he was an application chemist at Shimadzu Corporation Japan, and contributed to developing some novel analytical methods. His recent research has mainly focused on Automated Method Development, Workflow enhancement for Preparative LC, and Supercritical Fluid Chromatography. He has earned his Master&#39;s in Chemistry back in 2016.
</blockquote>
<blockquote>
Presenter: Liw Wan Tung (Assistant Product Manager (LC/Pharma), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Wan Tung, is the LC Product Manager and Pharmaceutical market in-charge at Shimadzu Asia Pacific Pte Ltd. She is the LC expert with more than 14 years of working experience in analytical science, specializing in method development of various LC and LCMS applications. In 2012, she joined Shimadzu to develop end-to-end workflow solutions for the customers. She has supported a multitude of applications from impurity analysis to drug discovery and biopharmaceutical applications. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honour&#39;s degree in chemistry back in 2009.
</blockquote>
<blockquote>
Presenter: Navin Devadiga, Ph.D (Assistant Manager (Drug Discovery), Shimadzu Analytical India)
</blockquote>
<blockquote>
Navin Devadiga currently works as an Assistant Manager at Shimadzu Analytical India, where he focuses on method development for the analytical division in the Drug Discovery segment. With over 14 years of experience in the analytical industry, he has gained specialized knowledge in advanced analytical instrumentation, particularly in LC, Preparative LC, LC-MS, and SFC technology. His responsibilities also include providing innovative solutions to drug discovery laboratories and pharmaceutical companies through the use of advanced analytical technologies. Prior to joining Shimadzu, Navin worked as an R&amp;D scientist at a multinational pharmaceutical company. During this time, he actively contributed to the development of analytical methods for identifying process-related impurities, degradation impurities, and genotoxic impurities in drug substances and drug products.
</blockquote>

Shimadzu: Streamlining Your Purification Workflow With Automation

Streamlining Your Purification Workflow With Automation

How Shimadzu's Nexera Prep can streamline your workflow and produce outstanding results with minimal effort and time.

Join us as we discuss the collaborative process that led from the introduction of Shimadzu’s Nexera UC analytical SFC systems to the Pharma-focused creation and launch of the Nexera UC Prep semi-preparative SFC systems.
We will explore the uses of SFC and see how the Shimadzu lineup has been shaped to meet the needs of Pharma SFC users through their direct input into instrument and software design and development.
We will host a Q&amp;A session after the presentation to address any questions or comments.
<blockquote>
Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.
</blockquote>
<blockquote>
Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.
</blockquote>

Shimadzu: Harnessing the Power of Collaboration: Shimadzu’s SFC Journey

Harnessing the Power of Collaboration: Shimadzu’s SFC Journey

The introduction of Shimadzu’s Nexera UC analytical SFC and the Pharma-focused Nexera UC Prep semi-preparative SFC systems

The Power of Collaboration: Shimadzu’s SFC Journey

Event Overview:
<ul>
<li>Principle of Chirality
</li>
<li>Chiral Stationary Phases (CSP)
</li>
<li>Polysaccharide CSPs and their Chiral Retention Mechanisms
</li>
<li>Chiral Method Development: Separation Modes and Screening Protocol
</li>
<li>SFC Chiral Method Development
</li>
<li>Chiral Method Optimization: Case Study
</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users who would like to understand how chiral separations work and how to apply the principles of chiral theory in developing an analytical method for separation of enantiomers.
</li>
<li>Any analyst who wishes to learn the basics of chiral theory.
</li>
<li>Any academic or pharmaceutical scientists who need to develop a method for separation and characterization of chiral compounds.
</li>
</ul>
Key Learning Points:
<ul>
<li>Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are
</li>
<li>Understand the three critical components of Polysaccharide CSPs and review the difference between coated and immobilized CSPs and how they are applied in different analytical contexts
</li>
<li>Learn how to approach a chiral screen using different separation modes.
</li>
</ul>
<blockquote>
Presenter: Laura K Ward (Technical Specialist, Phenomenex)
</blockquote>
<blockquote>
Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support. In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!
</blockquote>

Phenomenex: LC Chiral Theory and Method Development

LC Chiral Theory and Method Development

Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are.

Supercritical Fluid Chromatography (SFC), a chromatographic technique using a supercritical CO2 mobile phase, has been known for decades but was seldom used due to the popularity of normal and reverse phase liquid chromatography.Several years ago, SFC found a niche in chiral resolution when it was found that the physical characteristics of supercritical fluids can offer unique separations when compared to traditional liquid chromatography methods. Since then, these unique separations and “greener” aspect of CO2 have sparked new interest broadening SFC applications. Shimadzu, the leader in innovative SFC instrumentation, offers an array of analytical and preparative SFC systems that allow researchers easily access this growing technology. In this webinar, we’ll look at how Shimadzu’s SFC instruments and how our unique features are currently used in a variety of cutting edge applications for small and large molecules.<blockquote>Presenter: Stephen Swartz (Business Development Specialist, Shimadzu Scientific Instruments)Steve has been involved with the pharmaceutical industry for over 33 years. As a medicinal chemist at DuPont, Bayer, and Bristol Myers Squibb, he worked in multiple therapeutic areas supporting early phase discovery. After leaving the lab, Steve worked for several instrumentation companies in scientific support, sales, and business development. Since joining Shimadzu in 2020 as Business Development Specialist, he has worked with customers in the pharma and biopharma markets using his experience to help optimize workflows from early discovery to IND and beyond.Presenter:&nbsp;Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Shimadzu's Recent Advances in SFC

Shimadzu's Recent Advances in SFC

How Shimadzu’s SFC instruments and how our unique features are currently used in a variety of cutting edge applications for small and large molecules.

The complete Solution for Food Analysis: Presented by Retsch, CEM, Shimadzu and EltraAccurate and rapid food analysis is becoming increasingly important as regulations become stricter and consumers become more demanding. This expert day on food analysis will give you a comprehensive overview of the optimal steps from sample preparation to analysis, familiarize you with modern methods and bring you up to date.Registration is free of charge and without obligation.<div><div><div><div><div><h3>Heavy metals and pesticides in highly processed&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</h3>In the third part of the webinar series the focus is on analytics. Each step of food processing presents a source of contamination, which is why highly processed foods (ultra-processed foods (UPF)) should be viewed critically.Heavy metals are analyzed using ICP-MS and pesticides using GCMS/MS and SFC-LCMS/MS. You have the opportunity to get in touch with device experts and discuss your questions.</div></div>In this webinar, you will learn more about:<ul><li>How to measure pesticides in difficult samples using LC-MS/MS and SFC-MS/MS</li><li>Determination of pesticides in food samples using GC-MS/MS with hydrogen as carrier gas</li><li>Challenges and solutions for heavy metal analysis</li><li>How much heavy metals are lurking in trend food products?</li></ul>Presenters: Uwe Oppermann, Stéphane Moreau, Waldemar Weber and Johannes Hesper</div></div></div>

Heavy metals and pesticides in highly processed

Heavy metals are analyzed using ICP-MS and pesticides using GCMS/MS and SFC-LCMS/MS. 

Amgen Synthetics Separations Group supports several stages of the pipeline, from early drug discovery to commercial therapeutics. Chromatography technologies such as supercritical fluid chromatography (SFC) and high-performance liquid chromatography (HPLC) have been employed for various purification needs to support the portfolio throughout the years. But trends in drug development have focused on creating more complex and targeted molecules at the same time as a shift in embracing greener, more sustainable research and development practices.&nbsp;Our group has worked to overcome these new challenges by expanding chiral and achiral screening platforms, utilization of software-based method optimization and offline chromatographic modeling, and leveraging mass-directed purification systems for our parallel synthesis libraries. The results are reduced solvent consumption, increased efficiency, and enhanced selectivity for the standard workflows, and a creation of a ugHTPP scale purification platform.<blockquote>Presenter: Kevin Crossley (Scientist, Amgen)Kevin Crossley has worked in the pharmaceutical industry for 23 years and acquired extensive purification and chromatography experience. Kevin graduated from CSUN (California State University of Northridge) in 1998 with a B.A. in Biology with an emphasis in animal physiology. He began his scientific career as a bench chemist and purification scientist performing large scale extractions and isolations of natural products at Miravant Medical Technologies and later found an opportunity to work with Dr. Greg Cauchon, learning analytical techniques for the characterization of polymers and mAbs and renewing his scientific ambitions. In 2008, Kevin moved to Amgen supporting FIH through commercial products with impurity isolations, and large scale (multigram to kilogram) purifications of chiral and achiral molecules by SFC, RPLC and NPLC. In 2019, he joined Larry Miller’s group, utilizing a multitude of analytical and preparative skills to advance the Discovery pipeline and expand the purification capabilities. Kevin is a recipient of the EPA Green Chemistry Award (Greener Reaction Conditions) in the 2017 Green Chemistry Challenge, the Amgen SMP&amp;PD Green Chemistry Award in 2011, patent holder and contributor to several publications.&nbsp;</blockquote>

Agilent Technologies: Evolving Multiscale Separation Capabilities Towards High-Throughput Microgram Purification and Analysis

Evolving Multiscale Separation Capabilities Towards High-Throughput Microgram Purification and Analysis

Amgen Synthetics optimizes drug purification with advanced chromatography, greener practices, and software modeling for greater efficiency and selectivity.

Evolving Multiscale Separation for Microgram Purification

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. &nbsp;We will examine the break-through use of SFC with LabSolutions MD AQbD method development software to model and optimize peptide separations. &nbsp;The topic of Exposed Polar Surface Area analysis by SFC will be discussed and how its application to cyclic peptide therapeutics speeds up drug candidate selection. &nbsp;We will share recent developments in SFC separations for oligonucleotides, along with other enabling solutions for micro-prep SFC (analytical scale fractionation), hybrid UHPLC-SFC systems, and more.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Innovations in SFC Applications and Technology for Pharma and Biopharma

Innovations in SFC Applications and Technology for Pharma and Biopharma

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. 

LCMS webinars focusing on SFC | LabRulez LCMS