Solid Phase Extraction (SPE) is one of the most highly selective sample preparation methods, meaning that the targeted analytes and their matrices must be considered when choosing the correct SPE sorbent and format. Drying down the sorbent before elution is an important step.<ul><li>Wash solvents are used to flush out unwanted compounds (i.e., contaminants). If the wash solvent is not completely removed, then residual wash solvent could contaminate the final elution.</li><li>If you do not dry the SPE sufficiently, it could make the final solution less concentrated and result in a reduction in sensitivity.</li><li>Drying helps physically break down the interactions between the sorbent and analyte for a more concentrated extract.</li></ul>After the wash solvent has been passed through the cartridge, continue to apply negative pressure (vacuum) or positive pressure. Use this pressure to dry the cartridge for 2 to 5 minutes (or until dry). Turn off the pressure and wipe the tips of the manifold needles to remove any residual solvent.<h5>Notes</h5><ol><li>Reproducibility: Drying removes traces of residual sample/matrix and wash solvent from the sorbent. This produces a more concentrated final extract with a consistent volume and composition and yields better reproducibility and higher recoveries.</li><li>Immiscibility: Drying is especially critical when using elution solvents that are immiscible with water (e.g. hexane, ethyl acetate, and methylene chloride). Residual water in the sorbent bed may prevent dissolution of the analyte in the elution solvent, resulting in low recoveries and irreproducibility.</li><li>Over-drying: Avoid over-drying the sorbent, since in some cases, analyte recovery may decrease with excessive vacuum or drying times above 5 minutes. Dry cartridges look distinctly different from moist ones. Make a note of the cartridge appearance before and after conditioning. Also, you could touch the cartridge while it is drying; wet cartridges feel colder than dry cartridges.&nbsp;</li></ol>

Why Is It Necessary to Dry the SPE Sorbent Before Elution?

<h3>Mastering Ion-Pair Reversed-Phase Chromatography for Oligonucleotide Analysis</h3><h4>Why Purity Is Critical in the Oligonucleotide Era</h4>Oligonucleotides — short, synthetic strands of DNA or RNA — are central to modern biotechnology. They power gene therapies, advanced diagnostics, and cutting-edge molecular research. However, before these molecules can be used in clinical or research settings, their purity and structural integrity must be thoroughly verified. High-resolution chromatographic analysis plays a crucial role in ensuring quality and safety.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Separation_Example_of_Oligonucleotides_5577514561.jpg" alt="KNAUER: Figure 1 - Separation Example of Oligonucleotides." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Separation_Example_of_Oligonucleotides_5577514561.jpg 245w," sizes="100vw" width="1024" height="368"><h4>Understanding Ion-Pair Reversed-Phase (IP-RP) Chromatography</h4>For scientists involved in oligonucleotide synthesis and characterization, Ion-Pair Reversed-Phase Chromatography (IP-RP) is one of the most effective analytical tools available.Unlike separation techniques that rely solely on charge differences, IP-RP combines:<ul><li>Ionic interactions</li><li>Hydrophobic interactions</li></ul>This dual retention mechanism enables exceptional resolution — even for oligonucleotides that differ by only a single nucleotide or subtle chemical modification. Whether assessing synthesis efficiency or purifying therapeutic-grade material, IP-RP provides reliable, reproducible separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Overview_of_IP_RP_Mode_prinicple_ecd54b6e9c.jpg" alt="KNAUER: Figure 2 - Overview of IP-RP Mode prinicple." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Overview_of_IP_RP_Mode_prinicple_ecd54b6e9c.jpg 245w," sizes="100vw" width="1024" height="456"><h4>The Principle Behind Ion-Pairing</h4>Oligonucleotides possess a highly negatively charged phosphate backbone. In a traditional reversed-phase system, such hydrophilic, charged molecules would show little retention.IP-RP overcomes this limitation by introducing a positively charged ion-pairing reagent into the mobile phase. The cation associates with the negatively charged oligonucleotide, forming a transient ion pair with increased hydrophobic character. This modified complex can now interact with the hydrophobic stationary phase.Retention strength depends on:<ul><li>The hydrophobicity of the ion-pair reagent</li><li>The strength of ionic interaction</li><li>The applied organic gradient</li></ul>During gradient elution (commonly using acetonitrile), analytes are released in a controlled manner according to size, sequence, and chemical modifications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Ion_Pair_Reversed_Phase_Principle_9a4fcd12f2.jpg" alt="KNAUER: Figure 3 - Ion-Pair Reversed Phase Principle." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Ion_Pair_Reversed_Phase_Principle_9a4fcd12f2.jpg 245w," sizes="100vw" width="1024" height="393"><h4>Choosing the Right Ion-Pair Reagent</h4>Successful IP-RP separation depends heavily on selecting an appropriate ion-pair reagent. Two key factors determine retention behavior:<ol><li>Alkyl chain length</li><li>Charge density</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Ion_Pair_Classification_eaad172a4e.jpg" alt="KNAUER: Figure 4 - Ion-Pair Classification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Ion_Pair_Classification_eaad172a4e.jpg 245w," sizes="100vw" width="1024" height="398"><h4>Strong Cationic Ion-Pair Reagents</h4>Ion-pair reagents with longer alkyl chains provide stronger hydrophobic interactions and increased retention. A widely used example is tetrabutylammonium (TBA⁺), frequently applied for strongly retained anionic analytes.These reagents are particularly useful when high retention strength is required.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_Strong_Cationic_Ion_Pair_Reagents_8dbf00c57e.jpg" alt="KNAUER: Table 1 - Strong Cationic Ion-Pair Reagents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_Strong_Cationic_Ion_Pair_Reagents_8dbf00c57e.jpg 245w," sizes="100vw" width="1024" height="396"><h4>Volatile (Weaker) Ion-Pair Reagents</h4>Short-chain ion-pair reagents generate weaker retention but offer a significant advantage: compatibility with LC–MS detection.Triethylammonium (TEA⁺) is among the most commonly used reagents for oligonucleotide analysis, particularly when mass spectrometric detection is involved. Other options, such as diisopropylethylammonium (DIEA⁺), are also widely applied, often combined with HFIP to enhance MS performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_2_Weak_Cationic_Ion_Pair_Reagents_6332a4975e.jpg" alt="KNAUER: Table 2 - Weak Cationic Ion-Pair Reagents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_2_Weak_Cationic_Ion_Pair_Reagents_6332a4975e.jpg 245w," sizes="100vw" width="1024" height="283"><h4>Building an IP-RP Method for Oligonucleotides</h4>Developing a robust IP-RP method follows a logical sequence:<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_3_IP_RP_Method_Step_Procedure_for_Oligos_c2216c24a0.jpg" alt="KNAUER: Table 3 - IP-RP Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_3_IP_RP_Method_Step_Procedure_for_Oligos_c2216c24a0.jpg 245w," sizes="100vw" width="1024" height="304"><h5>1. Characterize the Analyte</h5>Oligonucleotides typically contain 10–50+ nucleotides and exhibit high negative charge density. This necessitates a suitable cationic partner such as TEA⁺ or DIEA⁺.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_Cationic_Ion_Pair_Principle_for_Oligonucleotides_cb2dfb7457.jpg" alt="KNAUER: Figure 5 - Cationic Ion-Pair Principle for Oligonucleotides." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_Cationic_Ion_Pair_Principle_for_Oligonucleotides_cb2dfb7457.jpg 245w," sizes="100vw" width="512" height="320"><h5>2. Select the Column</h5>Use a reversed-phase column with:<ul><li>Large pore size (≈300 Å)</li><li>Appropriate internal diameter</li></ul>These parameters support the so-called “ON–OFF” retention mechanism — a bind-and-elute behavior where analytes are strongly retained until the gradient triggers rapid elution.<h5>3. Optimize the Mobile Phase</h5>A typical starting condition includes:<ul><li>10 mM TEA buffer at pH ~7</li><li>Acetonitrile as organic modifier</li></ul>Gradient programs from approximately 5% to 50% organic solvent over 15–30 minutes are commonly effective for complex oligonucleotide mixtures.<h5>4. Adjust Retention Strength if Needed</h5>To increase retention:<ul><li>Raise ion-pair concentration (up to ~100 mM if required)</li><li>Select a more hydrophobic ion-pair reagent</li></ul><h5>5. Validate the Method</h5>Ensure robustness, sensitivity, and reproducibility by carefully evaluating:<ul><li>UV chromatograms</li><li>MS traces</li><li>Peak shape and resolution</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_UV_MS_traces_of_IP_RP_Oligonucleotide_Analysis_81faf4e9f0.jpg" alt="KNAUER: Figure 6 - UV-MS traces of IP-RP Oligonucleotide Analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_UV_MS_traces_of_IP_RP_Oligonucleotide_Analysis_81faf4e9f0.jpg 245w," sizes="100vw" width="1024" height="365"><h4>Why IP-RP Is Considered the Gold Standard</h4>Ion-Pair Reversed-Phase Chromatography uniquely combines the resolving power of reversed-phase LC with the ability to retain highly polar, charged biomolecules.Its advantages include:<ul><li>Tunable selectivity</li><li>Compatibility with UV and MS detection</li><li>Scalability from analytical to preparative workflows</li><li>Applicability from research to pharmaceutical production</li></ul>By transforming highly charged oligonucleotides into hydrophobic ion-pair complexes, IP-RP enables precise and reproducible separations that would otherwise be impossible in conventional reversed-phase systems.For these reasons, IP-RP is widely regarded as the benchmark technique for oligonucleotide analysis.<h4>Final Thoughts</h4>Mastering IP-RP is less intimidating than it may seem. A successful method comes down to:<ul><li>Selecting the right ion-pair reagent</li><li>Choosing a suitable column</li><li>Optimizing gradient conditions</li><li>Fine-tuning temperature and concentration parameters</li></ul>With thoughtful method development, IP-RP delivers sharp peaks, clean separations, and reliable performance — fully compatible with modern mass spectrometry workflows and ready to meet the demands of advanced oligonucleotide analysis.

Oligos Made Easy - Part 3: IP-RP

News from LabRulezLCMS Library - Week 08, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

BÜCHI

Agilent Technologies

Shimadzu Corporation

Waters Corporation

Supercritical Fluid Chromatography (SFC) offers several benefits over High-Performance Liquid Chromatography (HPLC) for compound isolation due to the nature of supercritical fluids.
During the webinar find out:
<ul>
<li>What Supercritical Fluids are, and how they are used for compound isolation</li>
<li>How SFC takes advantage of HPLC</li>
<li>Why SFC instruments are the green standard for compound isolation</li>
<li>When SFC can speed up a process</li>
</ul>
<blockquote>
Presenter: Holger Gumm (Managing Director, Sepiatec GmbH)
</blockquote>
<blockquote>
I’m the founder and Managing Director at Sepiatec, a new product line of compact and easy-to-use SFC instruments recently joined to BUCHI’s established portfolio.
</blockquote>
<blockquote>
Presenter: Birke Götz (Product Manager Purification, BUCHI Labortechnik)
</blockquote>
<blockquote>
I am a Global Product Manager for Purification at BUCHI Labortechnik AG and located in the headquarter in Flawil, Switzerland. 
</blockquote>

BUCHI: Why SFC? An in-depth look into the benefits of this separation method

Why SFC? An in-depth look into the benefits of this separation method

SFC offers several benefits over HPLC for compound isolation due to the nature of supercritical fluids.

Why SFC? An in-depth look into the benefits

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 2 | Basics of Supercritical Fluid Chromatography Purification</h3>
Supercritical Fluid Chromatography (SFC) is an effective and green technique to isolate a compound(s) of interest from a mixture. SFC utilizes liquid CO2 to quickly isolate compounds while minimizing the efforts required to dry the collected fraction. In this module, we will discuss factors involved in SFC purification. 
Learning Objectives: 
<ul>
<li>Understand how to use SFC purification</li>
<li>Make informed decisions on method optimization</li>
<li>Understand impact of decisions made during purification on larger workflow</li>
</ul>
<blockquote>
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
</blockquote>

Waters Corporation: Basics of Supercritical Fluid Chromatography Purification

Basics of Supercritical Fluid Chromatography Purification

We will discuss factors involved in SFC purification.

Experience faster, more efficient, and higher quality purification processes with Shimadzu&#39;s Preparative LC and SFC Solutions.
It’s time to streamline your workflow with Shimadzu&#39;s leading-edge Preparative LC and SFC solutions. Our Nexera Prep products offer complete solutions that produce clean and reproducible isolates, with a user-friendly workflow that accommodates all skill levels. Here&#39;s how Shimadzu&#39;s Nexera Prep can help you save time and boost productivity:
<ul>
<li>Nexera Prep Standard – Fully equipped with advanced functions that ensure reliable purification of target components.
</li>
<li>Nexera MDAP – Capable of high purity preparative work with no target components left behind.
</li>
<li>Nexera UFPLC – Equipped with trap technology for concentration, purification, and collection in 100% organic solvent, reducing overall processing time.
</li>
<li>ASAPrep – Automatic scale-up from analytical to preparative scale, saving time and resources needed for optimization.
</li>
</ul>
Join us in the live webinar to learn how Shimadzu&#39;s Nexera Prep can streamline your workflow and produce outstanding results with minimal effort and time. Don&#39;t miss out on this opportunity – save your seat now!
Learning Objectives:
<ul>
<li>Understand how purification workflow can be streamlined for higher efficiency
</li>
<li>Explore Shimadzu’s leading-edge Preparative LC and SFC solutions
</li>
<li>Learn how automation plays a role in eliminating tedious and manual work
</li>
</ul>
<blockquote>
Presenter: Kosuke Nakajima (Innovation Centre Manager, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Kosuke Nakajima has strong technical knowledge in separation science, particularly LC, LC/MS, and Preparative LC system. Before joining Shimadzu Asia Pacific as an Innovation Centre Manager, he was an application chemist at Shimadzu Corporation Japan, and contributed to developing some novel analytical methods. His recent research has mainly focused on Automated Method Development, Workflow enhancement for Preparative LC, and Supercritical Fluid Chromatography. He has earned his Master&#39;s in Chemistry back in 2016.
</blockquote>
<blockquote>
Presenter: Liw Wan Tung (Assistant Product Manager (LC/Pharma), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Wan Tung, is the LC Product Manager and Pharmaceutical market in-charge at Shimadzu Asia Pacific Pte Ltd. She is the LC expert with more than 14 years of working experience in analytical science, specializing in method development of various LC and LCMS applications. In 2012, she joined Shimadzu to develop end-to-end workflow solutions for the customers. She has supported a multitude of applications from impurity analysis to drug discovery and biopharmaceutical applications. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honour&#39;s degree in chemistry back in 2009.
</blockquote>
<blockquote>
Presenter: Navin Devadiga, Ph.D (Assistant Manager (Drug Discovery), Shimadzu Analytical India)
</blockquote>
<blockquote>
Navin Devadiga currently works as an Assistant Manager at Shimadzu Analytical India, where he focuses on method development for the analytical division in the Drug Discovery segment. With over 14 years of experience in the analytical industry, he has gained specialized knowledge in advanced analytical instrumentation, particularly in LC, Preparative LC, LC-MS, and SFC technology. His responsibilities also include providing innovative solutions to drug discovery laboratories and pharmaceutical companies through the use of advanced analytical technologies. Prior to joining Shimadzu, Navin worked as an R&amp;D scientist at a multinational pharmaceutical company. During this time, he actively contributed to the development of analytical methods for identifying process-related impurities, degradation impurities, and genotoxic impurities in drug substances and drug products.
</blockquote>

Shimadzu: Streamlining Your Purification Workflow With Automation

Streamlining Your Purification Workflow With Automation

How Shimadzu's Nexera Prep can streamline your workflow and produce outstanding results with minimal effort and time.

Join us as we discuss the collaborative process that led from the introduction of Shimadzu’s Nexera UC analytical SFC systems to the Pharma-focused creation and launch of the Nexera UC Prep semi-preparative SFC systems.
We will explore the uses of SFC and see how the Shimadzu lineup has been shaped to meet the needs of Pharma SFC users through their direct input into instrument and software design and development.
We will host a Q&amp;A session after the presentation to address any questions or comments.
<blockquote>
Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.
</blockquote>
<blockquote>
Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.
</blockquote>

Shimadzu: Harnessing the Power of Collaboration: Shimadzu’s SFC Journey

Harnessing the Power of Collaboration: Shimadzu’s SFC Journey

The introduction of Shimadzu’s Nexera UC analytical SFC and the Pharma-focused Nexera UC Prep semi-preparative SFC systems

The Power of Collaboration: Shimadzu’s SFC Journey

Event Overview:
<ul>
<li>Principle of Chirality
</li>
<li>Chiral Stationary Phases (CSP)
</li>
<li>Polysaccharide CSPs and their Chiral Retention Mechanisms
</li>
<li>Chiral Method Development: Separation Modes and Screening Protocol
</li>
<li>SFC Chiral Method Development
</li>
<li>Chiral Method Optimization: Case Study
</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users who would like to understand how chiral separations work and how to apply the principles of chiral theory in developing an analytical method for separation of enantiomers.
</li>
<li>Any analyst who wishes to learn the basics of chiral theory.
</li>
<li>Any academic or pharmaceutical scientists who need to develop a method for separation and characterization of chiral compounds.
</li>
</ul>
Key Learning Points:
<ul>
<li>Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are
</li>
<li>Understand the three critical components of Polysaccharide CSPs and review the difference between coated and immobilized CSPs and how they are applied in different analytical contexts
</li>
<li>Learn how to approach a chiral screen using different separation modes.
</li>
</ul>
<blockquote>
Presenter: Laura K Ward (Technical Specialist, Phenomenex)
</blockquote>
<blockquote>
Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support. In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!
</blockquote>

Phenomenex: LC Chiral Theory and Method Development

LC Chiral Theory and Method Development

Learn what chiral compounds are and how they are separated using LC and what a chiral stationary phase (CSP) is and the different types there are.

Supercritical Fluid Chromatography (SFC), a chromatographic technique using a supercritical CO2 mobile phase, has been known for decades but was seldom used due to the popularity of normal and reverse phase liquid chromatography.Several years ago, SFC found a niche in chiral resolution when it was found that the physical characteristics of supercritical fluids can offer unique separations when compared to traditional liquid chromatography methods. Since then, these unique separations and “greener” aspect of CO2 have sparked new interest broadening SFC applications. Shimadzu, the leader in innovative SFC instrumentation, offers an array of analytical and preparative SFC systems that allow researchers easily access this growing technology. In this webinar, we’ll look at how Shimadzu’s SFC instruments and how our unique features are currently used in a variety of cutting edge applications for small and large molecules.<blockquote>Presenter: Stephen Swartz (Business Development Specialist, Shimadzu Scientific Instruments)Steve has been involved with the pharmaceutical industry for over 33 years. As a medicinal chemist at DuPont, Bayer, and Bristol Myers Squibb, he worked in multiple therapeutic areas supporting early phase discovery. After leaving the lab, Steve worked for several instrumentation companies in scientific support, sales, and business development. Since joining Shimadzu in 2020 as Business Development Specialist, he has worked with customers in the pharma and biopharma markets using his experience to help optimize workflows from early discovery to IND and beyond.Presenter:&nbsp;Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Shimadzu's Recent Advances in SFC

Shimadzu's Recent Advances in SFC

How Shimadzu’s SFC instruments and how our unique features are currently used in a variety of cutting edge applications for small and large molecules.

The complete Solution for Food Analysis: Presented by Retsch, CEM, Shimadzu and EltraAccurate and rapid food analysis is becoming increasingly important as regulations become stricter and consumers become more demanding. This expert day on food analysis will give you a comprehensive overview of the optimal steps from sample preparation to analysis, familiarize you with modern methods and bring you up to date.Registration is free of charge and without obligation.<div><div><div><div><div><h3>Heavy metals and pesticides in highly processed&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;</h3>In the third part of the webinar series the focus is on analytics. Each step of food processing presents a source of contamination, which is why highly processed foods (ultra-processed foods (UPF)) should be viewed critically.Heavy metals are analyzed using ICP-MS and pesticides using GCMS/MS and SFC-LCMS/MS. You have the opportunity to get in touch with device experts and discuss your questions.</div></div>In this webinar, you will learn more about:<ul><li>How to measure pesticides in difficult samples using LC-MS/MS and SFC-MS/MS</li><li>Determination of pesticides in food samples using GC-MS/MS with hydrogen as carrier gas</li><li>Challenges and solutions for heavy metal analysis</li><li>How much heavy metals are lurking in trend food products?</li></ul>Presenters: Uwe Oppermann, Stéphane Moreau, Waldemar Weber and Johannes Hesper</div></div></div>

Heavy metals and pesticides in highly processed

Heavy metals are analyzed using ICP-MS and pesticides using GCMS/MS and SFC-LCMS/MS. 

Amgen Synthetics Separations Group supports several stages of the pipeline, from early drug discovery to commercial therapeutics. Chromatography technologies such as supercritical fluid chromatography (SFC) and high-performance liquid chromatography (HPLC) have been employed for various purification needs to support the portfolio throughout the years. But trends in drug development have focused on creating more complex and targeted molecules at the same time as a shift in embracing greener, more sustainable research and development practices.&nbsp;Our group has worked to overcome these new challenges by expanding chiral and achiral screening platforms, utilization of software-based method optimization and offline chromatographic modeling, and leveraging mass-directed purification systems for our parallel synthesis libraries. The results are reduced solvent consumption, increased efficiency, and enhanced selectivity for the standard workflows, and a creation of a ugHTPP scale purification platform.<blockquote>Presenter: Kevin Crossley (Scientist, Amgen)Kevin Crossley has worked in the pharmaceutical industry for 23 years and acquired extensive purification and chromatography experience. Kevin graduated from CSUN (California State University of Northridge) in 1998 with a B.A. in Biology with an emphasis in animal physiology. He began his scientific career as a bench chemist and purification scientist performing large scale extractions and isolations of natural products at Miravant Medical Technologies and later found an opportunity to work with Dr. Greg Cauchon, learning analytical techniques for the characterization of polymers and mAbs and renewing his scientific ambitions. In 2008, Kevin moved to Amgen supporting FIH through commercial products with impurity isolations, and large scale (multigram to kilogram) purifications of chiral and achiral molecules by SFC, RPLC and NPLC. In 2019, he joined Larry Miller’s group, utilizing a multitude of analytical and preparative skills to advance the Discovery pipeline and expand the purification capabilities. Kevin is a recipient of the EPA Green Chemistry Award (Greener Reaction Conditions) in the 2017 Green Chemistry Challenge, the Amgen SMP&amp;PD Green Chemistry Award in 2011, patent holder and contributor to several publications.&nbsp;</blockquote>

Agilent Technologies: Evolving Multiscale Separation Capabilities Towards High-Throughput Microgram Purification and Analysis

Evolving Multiscale Separation Capabilities Towards High-Throughput Microgram Purification and Analysis

Amgen Synthetics optimizes drug purification with advanced chromatography, greener practices, and software modeling for greater efficiency and selectivity.

Evolving Multiscale Separation for Microgram Purification

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. &nbsp;We will examine the break-through use of SFC with LabSolutions MD AQbD method development software to model and optimize peptide separations. &nbsp;The topic of Exposed Polar Surface Area analysis by SFC will be discussed and how its application to cyclic peptide therapeutics speeds up drug candidate selection. &nbsp;We will share recent developments in SFC separations for oligonucleotides, along with other enabling solutions for micro-prep SFC (analytical scale fractionation), hybrid UHPLC-SFC systems, and more.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Innovations in SFC Applications and Technology for Pharma and Biopharma

Innovations in SFC Applications and Technology for Pharma and Biopharma

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. 

Innovations in SFC Applications in (Bio)Pharma 

Supercritical fluid chromatography (SFC) is gaining renewed attention for its sustainable, cost-effective, and highly sensitive capabilities—offering the potential to outperform traditional HPLC and GC methods.In this latest session of Digital Classrooms, join our experts, Derrick Tan and Chua Chee Keong, as they explore the principles and diverse applications of supercritical fluid extraction (SFE) and SFC, with a focus on achieving precise and efficient separations. Experience the innovation of Shimadzu’s Nexera UC SFE/SFC system, which redefines performance standards with fully automated on-line extraction and separation, along with an eco-friendly performance that reduces solvent usage by 94.2% compared to normal-phase methods.Complementing the leading-edge system is our extensive lineup of chiral and achiral SFC columns, including the newly released ShimOMNIA column, designed for exceptional enantioselectivity and robust performance. Additionally, our collaboration with Core Separations enables seamless scalability, taking your workflow from research to industrial-scale production. This partnership delivers a fully integrated, end-to-end solution to meet all your separation needs.Don’t miss this opportunity to elevate your expertise—Register today and secure your spot!<h4>Learning Objectives:</h4><ul><li>Gain insights on the use of Nexera UC system for analytical to preparative SFC separation.</li><li>Explore the wide range of Shimadzu SFC columns for optimum achiral and chiral separation.</li><li>Discover the capabilities of small to industrial scale SFE from Core Separation.</li></ul><blockquote>Presenter: Derrick Tan (Senior Application Chemist, Shimadzu (Asia Pacific) Pte Ltd)Derrick obtained his PhD in Chemistry from Nanyang Technological University, Singapore. With over 7 years of extensive research experience, Dr Derrick has specialized in various areas, including small molecules, nucleic acids, peptides, and protein synthesis, as well as separation and purification techniques. He joined Shimadzu (Asia Pacific) Pte Ltd in 2021 as an Application Chemist (Consumables). In this role, he is responsible for providing technical support and application expertise for consumables in chromatography and spectroscopy. Portfolio under his care includes a wide range of products, such as LC and GC columns, UV/Vis cuvettes, sample preparation products, sample vials, and other accessories.Presenter: Chua Chee Keong (Product Specialist (LC), Shimadzu (Asia Pacific) Pte Ltd)Chee Keong is the LC/SFC Product Specialist at Shimadzu Asia Pacific with more than five years of experience in analytical science. He specializes in providing technical consultation to researchers and laboratory professionals, helping them optimize methodologies and achieve precise results. Prior to joining Shimadzu, he gained valuable experience across diverse industries, including oil and gas, pharmaceuticals, and life sciences. His expertise spans from laboratory operations and analytical methodologies, with a strong focus on active pharmaceutical ingredient (API) analysis, formulation development, and method transfer to manufacturing, ensuring seamless scalability and precision in production. Chee Keong holds a bachelor’s degree in Analytical Chemistry from Universiti Sains Malaysia, (USM).</blockquote>

Shimadzu: Advancing Separation Science: SFE/SFC For Chiral & Achiral Analysis

Advancing Separation Science: SFE/SFC For Chiral & Achiral Analysis

Discover SFE/SFC principles, Nexera UC automation, and Shimadzu column options for precise, scalable, and eco-friendly separations. Register for this session.

LCMS webinars focusing on SFC - page 1