Thirteenth annual Conference of the Czech Society for Mass Spectrometry will take place together with the eleventh Informal Proteomic Meeting from November 19 – November 21, 2025 in České Budějovice.<h3>Registration</h3><blockquote><ul><li>Registration is only possible on site.&nbsp;</li><li>Registration fee: CZK 2500.</li></ul></blockquote><h3>Program</h3><blockquote>The complete program, including links to lecture abstracts, can be found HERE:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6384">Program of the 13th Conference of Czech Mass Spectrometry together with 11th Informal proteomic meeting</a></li></ul></blockquote><h3>List of posters (including links to their abstracts):</h3><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=4124815">WeS-01:</a> Quantification of Oxoacids of the Human Metabolome using Liquid Chromatography-Mass Spectrometry<ul><li>Philipp Hebesberger</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=9840543">WeS-02</a>: Analyses of human scent samples<ul><li>Veronika Škeříková</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5983681">WeS-03</a>: rDUVLAESCI-MS/MSI: a novel approach for direct analysis of biological surfaces<ul><li>Jakub Grepl</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=8595826">WeS-04</a>: Investigation of phenolic compounds in grape pomace through UHPLC-ESI-MS/MS<ul><li>Michal Jágr</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7046316">WeS-05</a>: Mass Spectral Library Network (MERLIN) to streamline the generation of public spectral libraries<ul><li>Federico Brigante</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5455392">WeS-06</a>: A comprehensive timsTOF MS/MS spectral library for improving metabolite annotation in non-targeted metabolomics<ul><li>Aninda Mazumdar</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=3643222">ThS-07</a>: Propionic acid boosts sensitivity in high-flow RPLC-MS proteomics compared to formic and acetic acid<ul><li>Juraj Lenčo</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=8104565">ThS-08</a>: Towards biomarker discovery: Multiomics analysis of endometriosis<ul><li>Tomáš Oždian</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6375703">ThS-09</a>: Integrated proteogenomic analysis identifies synuclein gamma as a key target of the mesenchymal stem-like subtype of triple negative breast cancer<ul><li>Jan Šimoník</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5382082">ThS-10</a>: Human IMPs: A tool for comparing outputs of different tools for transmembrane segment predictions in the human proteome<ul><li>Ondřej Vít</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6248138">ThS-11</a>: LC-MS/MS-based terminomics unravels role of carboxypeptidase B1 in luminal A breast cancer<ul><li>Petr Lapcik</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=9618906">ThS-12</a>: Mass spectrometry in hands of experimental biology: Impaired nucleotide salvage affects de novo synthesis in Drosophila melanogaster<ul><li>Jakub Sysel</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5574795">ThS-13</a>: Bioaccumulation of pharmaceuticals in herbivorous fish: The role of aquatic plants as a dietary source<ul><li>Helena Hinterholzová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=8545306">ThS-14</a>: LC-HRMS for large-scale chemical exposure studies<ul><li>Štěpán Koudelka</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7597199">ThP-01</a>: Complexome profiling workflow elucidates mitochondrial cytochrome c oxidase biogenesis<ul><li>Marek Vrbacky</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=2382300">ThP-02</a>: Amino acid profiling across diverse biological matrices using the MetAmino® LC-MS Kit: Applications in free and peptide-bound amino acid analysis<ul><li>Stanislav Opekar</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=8863587">ThP-03</a>: Ambient MS for rapid screening of pharmaceutically active compounds: Off-the-shelf and lab-made ion sources<ul><li>Alžběta Němeškalová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=3765372">ThP-04</a>: Adaptive proteome underpins drought tolerance in bread wheat during flowering<ul><li>Olha Lakhneko</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=1069383">ThP-05</a>: Transition from natural dyestuffs to synthetic colorants depicted by ion mobility-aided mass spectrometry<ul><li>Volodymyr Pauk</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=3752972">ThP-06</a>: Off-line GC–GC×GC-MS focusing on scent samples<ul><li>Ulrika Malá</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7593891">ThP-07</a>: Ambient soft landing voltammetry: Tool for electrode modification and molecular redox characterization<ul><li>Adam Neuman</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7978669">ThP-08</a>: Translational research aimed at improving heatstroke diagnosis<ul><li>Andrej Kovac</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=2838864">ThP-09</a>: Tracing the Gravettian stone paths<ul><li>Filip Gregar</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=4304936">ThP-10</a>: Leaf discs as a suitable alternative for cell cultures in plant omics analyses<ul><li>Petr Čičmanec</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=4353606">ThP-11</a>: Characterization of nanoparticles in mixtures by Taylor dispersion analysis hyphenated to inductively coupled plasma mass spectrometry<ul><li>Tomáš Pluháček</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6271919">ThP-12</a>: Sub-cellular interacting partners of the tick-borne encephalitis virus capsid protein<ul><li>Kateřina Kubátová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7486753">ThP-13</a>: Mass spectrometry-based proteomic profiling of Sugiyamaella lignohabitans adaptation to xylose as carbon source<ul><li>Jana Bellová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=3044553">ThP-14</a>: Proteomic analysis of acetylsalicylic acid-induced changes in the cellular, secreted, and extracellular vesicle proteome of malignant melanocytes<ul><li>Jakub Červenka</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=1444237">ThP-15</a>: Apparatus for protein footprinting by singlet oxygen<ul><li>Lada Fialová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7301517">ThP-16</a>: Insight into hemoglobin digestion pathways of Sphaerospora molnari by functional proteomics<ul><li>Filip Dyčka</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=4960125">ThP-17</a>: High-flow HESI interface with point-focusing and enhanced desolvation<ul><li>Jana Knytlová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6499455">ThP-18</a>: Proteomics identifies the landmark protein expression difference among mutation-based clusters of human pheochromocytoma and paraganglioma<ul><li>Tereza Hrdinová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=3701074">ThP-19</a>: Application of LC–MS/MS and fluorescence-based assays in the evaluation of novel carbonic anhydrase inhibitors<ul><li>Mária Dobšovičová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=4121623">ThP-20</a>: Production of sialylated glycoproteins in Ixodes ricinus ticks and tick cell lines<ul><li>Jarmila Štěrbová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7502272">ThP-21</a>: Application of LC–MS-based “polaromics” in biochemistry, pharmacology, and biomedicine<ul><li>Jaroslav Galba</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=2719813">ThP-22</a>: Development of software for LC-MS data processing of oligonucleotides<ul><li>Evgeniya Biryukova</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7174479">ThP-23</a>: Enhanced bottom-up proteomics for histone peptidoform profiling<ul><li>Palina Ryzhaya</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=2072360">ThP-24</a>: Detection of deoxynivalenol in grain products by LC-MS<ul><li>Daniel Smíšek</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7139436">ThP-25</a>: FAmOS versus Percoll: Advancing organelle isolation efficiency and proteomic compatibility in plant research<ul><li>Ivo Chamrád</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6175084">ThP-26</a>: Quantification of monoclonal antibodies by middle-up capillary electrophoresis coupled to mass spectrometry<ul><li>Jana Havlíková</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7843229">ThP-27</a>: Integrative proteomic and hormonomic analysis of barley chloroplast mutant chlorina f2f2<ul><li>Natálie Zemánková</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=9933055">ThP-28</a>: Microplastics and the blood–brain barrier: Mechanisms of transport and endothelial response<ul><li>Petra Majerová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=1637886">ThP-29</a>: Reactivity of singlet oxygen toward amino acid side chains<ul><li>Marta Cruz</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5725330">ThP-30</a>: Applied and residual: Assessment of pesticide residues and transformation products in irrigated Czech agricultural fields<ul><li>Alina Sadchenko</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5136513">ThP-31</a>: Expanding the FFAP protocol for structural characterization of membrane proteins<ul><li>Michael Karpíšek</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=1961961">ThP-32</a>: DigDig: Streamlining digestion analysis for proteomics and HDX-MS<ul><li>Zuzana Kalaninová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5856285">ThP-33</a>: Illuminating proteins: Dual UV/IR photodissociation in a custom FT-ICR system<ul><li>Ezgi Gurler</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=9770654">ThP-34</a>: Decoding therapy-induced protein subcellular relocalization in leukemia<ul><li>Michaela Myšáková</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=5337771">ThP-35</a>: Advancing AI/ML-based post-translational modification assignment using a verified FPOP dataset<ul><li>Dmitry Loginov</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=2599017">ThP-36</a>: Analysis of glycerophospholipids using the method of direct infusion into a tribrid mass spectrometer with high mass resolution<ul><li>Petr Zacek</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=1028179">ThP-37</a>: In situ comparison of hormonomics changes in different biotic stresses using mass spectrometry imaging<ul><li>Chao Zhang</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6619097">ThP-38</a>: Large-scale metabolomics screening of understudied plant lineages<ul><li>Kateřina Kučerová</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=9282171">ThP-39</a>: Quantitative and redox proteomics identify signaling networks shaping leukemia survival and resistance<ul><li>Dušan Nemes</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=6336342">ThP-40</a>: Deciphering phlebotomine sand fly taxonomy using MALDI-TOF MS protein profiling<ul><li>Petr Halada</li></ul><a target="_blank" rel="noopener noreferrer" href="https://www.czechms.org/cz/events/cmsc/abstract.php?code=7924365">ThP-41</a>: Metabolomics analysis of type 1 diabetes mellitus among Qatari children using LC-MS<ul><li>Haya Alsulaiti</li></ul>

Poster section of the 13th Conference of Czech Mass Spectrometry together with 11th Informal proteomic meeting

A research team at IOCB Prague led by Jiří Kaleta has described a new concept for light-driven molecular motors that remain fully functional even after being anchored to a metal surface, where they continue to perform controlled rotary motion. The scientists have thus overcome one of the main obstacles on the road to fully controllable molecular machines – a development expected to have a major impact on the creation of intelligent materials, among other applications. The study provides deeper insight into the control of motion at the nanoscale. The paper was published in the journal Angewandte Chemie International Edition.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Tomas_Bellon_UOCHB_Dr_Jiri_Kaleta_vedouci_vedecke_skupiny_Molekularni_stroje_UOCHB_4585ced86b.jpg" alt="Tomáš Belloň / IOCB Prague: Dr. Jiří Kaleta, head of the Molecular Devices group at IOCB Prague" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Tomas_Bellon_UOCHB_Dr_Jiri_Kaleta_vedouci_vedecke_skupiny_Molekularni_stroje_UOCHB_4585ced86b.jpg 234w," sizes="100vw" width="1600" height="1068">The study of molecular motors is among the most fascinating areas of contemporary chemistry and nanotechnology. Comprising only a few dozen atoms, these machines can move or rotate in a precisely defined direction when exposed to an external stimulus, such as light. The team at IOCB has developed two types of so-called four-legged motors attached to a rigid triptycene base. Triptycene is a three-dimensional structure that holds the stationary part of the motor slightly above the surface, allowing the rotor to move freely and in a precisely controlled manner. Upon exposure to light, the rotor first makes a partial turn and then completes the rotation through the influence of heat, resulting in a full, controlled 360° rotation. Kateřina Bezděková, the first author of the study and a member of <a target="_blank" rel="noopener noreferrer" href="https://kaleta.group.uochb.cz/en">Kaleta’s research group</a>, explains: „Our motors are designed like building blocks, making them easy to adjust for different applications. We show that controlled motion at the molecular level can not only be achieved but also modified.”Jiří Kaleta’s team succeeded in overcoming one of the major technical challenges: molecules attached to solid surfaces often lose their ability to move due to excessively tight contact with the substrate. Thanks to the innovative design, the motors remain mobile under all conditions. “By using a rigid triptycene base, we managed to solve several problems,” explains Jiří Kaleta. “Among other things, we effectively decoupled the photoactive part of the motor from the surface. As a result, it retains its mobility and can be easily controlled by light.”The results from IOCB Prague pave the way for the practical use of molecular machines, for example in the development of intelligent materials, photoactive switches, or surface sensors. “Every step that improves our ability to control motion at the atomic level brings us closer to the time when molecular machines will perform precisely defined tasks – just like the machines we know from the macroscopic world, only a billion times smaller,” adds Jiří Kaleta.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Molecular_motors_aa40e38cb0.jpg" alt="IOCB Prague: Two tetrapodal triptycene-based light-driven molecular motors with precisely oriented rotation axes—parallel or perpendicular to a gold surface—achieve full 360° rotation in both solution and as monolayers. The design minimizes surface quenching, enabling functional integration into surface-mounted nanomachines." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Molecular_motors_aa40e38cb0.jpg 209w," sizes="100vw" width="667" height="498"><blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://onlinelibrary.wiley.com/doi/10.1002/anie.202513922">Surface-Decoupled Altitudinal and Azimuthal Triptycene-Fused Tetrapodal Molecular Motors</a></h4>Kateřina Bezděková, Lukáš Severa, Eva Kaletová, Katarina Majerová Varga, Milan Mašát, Liang-Ting Wu, Jyh-Chiang Jiang, Ivana Císařová, Jiří KaletaAngew. Chem., Int. Ed. 2025.<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/anie.202513922">https://doi.org/10.1002/anie.202513922</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Two light-driven molecular motors, fused to a triptycene-based tetrapodal platform, with rotational axes oriented either parallel or perpendicular to the surface, were successfully designed and synthesized. Both systems demonstrated complete 360° rotation cycles, efficient photoswitching at 385 ± 5 nm (reaching ∼90% at the photostationary state), and quantitative thermal helix inversion with half-lives of ∼7 min at 20 °C. When assembled as monolayers on gold surfaces, the motors retained their full rotational functionality, demonstrating the ability of the tetrapodal platform to minimize surface interactions. These findings highlight the potential of these systems for applications in surface-integrated molecular devices and machines.

IOCB researchers take a key step toward fully controllable molecular machines

Join us for an interesting interview with Taylor Bias, a leading researcher in the field of mitochondrial proteins, as she discusses her groundbreaking work on mitoNEET and its implications for diseases like Type 2 Diabetes, Parkinson's Disease, and Cancer. In this engaging conversation with Organomation General Manager David Oliva, Taylor shares the challenges she faced in developing analytical methods to study mitoNEET's enzymatic activities and the pivotal role of chromatography techniques like GC-MS and HPLC in her research.Discover how chromatography is evolving in the study of complex proteins and hear about some unexpected findings that have emerged from Taylor's research. For young researchers eager to dive into interdisciplinary studies, Taylor offers valuable advice on navigating this exciting field. Additionally, she addresses common misconceptions about mitoNEET and clarifies its significant role in disease mechanisms. Don't miss this opportunity to learn from an expert about the fascinating intersection of biochemistry, disease research, and advanced analytical techniques!<h3>Video Transcription</h3><h5>Taylor Bias:</h5>I'm a senior at Ball State. I have a chemistry major with a biochemistry concentration and a biology minor. I've been in my current biochemistry group for about three years now, and I study the protein MitoNEET.It's a pretty novel protein, discovered in 2004 as part of a screening for diabetes drugs. Its increased regulation and decreased levels have also been linked to Parkinson's disease and different types of cancer.I study the protein as an enzyme. I first studied it in an oxidation reaction with cysteine to cystine, and then as a transaminase enzyme with the cofactor PLP, where we switch functional groups on cysteine and 2-oxoglutarate to make the new amino acid glutamate and the new alpha-keto acid thiocaptoproprionate. I look at both of those reactions on the GC-MS or the HPLC.<h5>Interviewer: Very cool. What initially sparked your interest in studying MitoNEET, and how has that research focus evolved over time?</h5><h5>Taylor Bias:</h5>Originally, I kind of just joined the lab for its supportive environment and the people I knew, but I've come to really like this research. I think it's very exciting to be the only person who knows something about a protein that's about the same age as me.Anytime I run a new reaction, I get to know something about this protein before anyone else does, and I think that's really cool. Every protein is unique in the way that you need to work with it and analyze its reactions.Our research has changed quite a bit in the three years I've been here. Originally we were just trying to see: Can it act as an enzyme at all? Then, Can it act as specific types of enzymes?—which led to uncovering that transaminase activity.Now we've shifted toward: How can we change and affect the activity? Can we make it faster? Can we make it slower? What will it react with? What won’t it react with? It's been really cool to see.<h5>Interviewer: What challenges did you face in developing analytical methods to study MitoNEET’s enzymatic activities? Why was it useful to utilize both GC-MS and HPLC?</h5><h5>Taylor Bias:</h5>GC-MS and HPLC were both necessary because thiocaptoproprionate in the transaminase reaction can only be seen in certain ways. On the GC-MS, you can only see things that will derivatize with TBDMS and that will fly through the instrument as a gas. Thiocaptoproprionate has a really hard time doing that—it's very hard to quantify.So we developed an HPLC method, which had its own set of challenges: figuring out what would work, what we had to change compared to GC-MS.At one point, we encountered a major challenge where our samples were just losing material like crazy. They were super inconsistent between me and our Master's student, Joey Maran, who's now getting his PhD at Notre Dame.We had to go through every step of the method to figure out where something could go wrong. There are a lot of moving parts, and in research things don’t always work perfectly.Eventually, we found that our reaction wasn’t stopping completely when we heated it to denature the enzyme. We had to add a certain amount of acid to completely stop the reaction so our counts would be consistent again. We implemented that into both the GC-MS and HPLC methods, and it helped—our results are way more consistent now.<h5>Interviewer: How do you see the role of chromatography evolving in the study of proteins like MitoNEET?</h5><h5>Taylor Bias:</h5>The unique thing about MitoNEET—and similar proteins—is that their enzymatic activity invites a lot of analytical methods like GC-MS or HPLC. With those instruments, you can look at what's in the material, what's in our sample.Letting the enzyme react with substrates to make products, it's nice to use these instruments to see: What are we making? In what amounts?For MitoNEET specifically, we're developing new LC-MS/MS methods to get mass-spec data for the compounds harder to quantify on GC-MS, like thiocaptoproprionate.For other enzymatic proteins, it's also easier to quantify results when you use analytical tools like GC-MS or HPLC.<h5>Interviewer: Could you discuss any unexpected findings or surprises you encountered during your research?</h5><h5>Taylor Bias:</h5>Using these instruments, there are lots of surprises. Things don’t always go as planned. During my first summer of research, we ran into issues with our GC-MS. It would run the reaction and look like it was doing everything it was supposed to do, but then during analysis all of our graphs—the fragmentations, the TIC—just weren't there. No data.It took effort to figure out what was wrong. I ended up babysitting the instrument for a couple of runs, watching what was and wasn’t happening. It turned out our ion source was out. We needed to replace it.That was really cool for me because my research is mostly biochemistry and analytical work—not so much mechanical instrument work. So getting to open the instrument, take out parts, replace them, and see our data return was very exciting.<h5>Interviewer: What advice would you give to young researchers interested in interdisciplinary studies?</h5><h5>Taylor Bias:</h5>Go for it. Get involved. Try everything you possibly can.Interdisciplinary work is great because you can explore lots of things and see what you like and don’t like. Undergrad is the perfect time to try things, succeed at them, fail at them—there’s never going to be a better point in your career to explore.Be curious. Ask questions. Get involved in everything you can. Learn what you can while you can.<h5>Interviewer: Are there any misconceptions about MitoNEET or its role in disease that you’d like to address?</h5><h5>Taylor Bias:</h5>MitoNEET is pretty novel—discovered only in 2004—so there’s not a lot of information out there yet. I wouldn’t say there are many misconceptions.It’s a crucial part of reactive oxygen species control, being part of the mitochondria. It’s involved in pathways with both pros and cons. When MitoNEET is missing or upregulated, we get diseases like diabetes, cancer, and Parkinson’s. But it’s also needed for our bodies to function.It’s a complicated protein with positives and negatives, but I’m personally a big fan.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Exploring MitoNEET: Unraveling Its Role in Type 2 Diabetes, Parkinson's, and Cancer with Taylor Bias

Article

Events

Video

Scientists

Scientific article

Interview

Science and research

The relatively new tradition of the Czech mass spectrometry conferences was established only in 2011, the very first event took place in Hradec Králové. Since then, the conference was organized every year except for 2020, the year of the pandemic.

Czech Mass Spectrometry Conferences

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

BÜCHI

Biotage

Phenomenex

Ever wished certain tasks could be automated? Everything can be done with just a few clicks these days, right? Surely the same applies to chromatography.
On the 6th of June, we are introducing the ideal instrument to help you take the first steps into the world of automated flash chromatography.
Be the first to find out just how simple chromatography can be.

BUCHI: Product Launch: Pure Essential Chromatography

Product Launch: Pure Essential Chromatography

On the 6th of June, we are introducing the ideal instrument to help you take the first steps into the world of automated flash chromatography.

Attendees will see how currently available technologies can provide major solvent (and time) savings and improve their workflow and productivity.
<h5>This presentation provides easy to do suggestions to reduce organic solvent use including:</h5>
<ul>
<li>Step gradients</li>
<li>Smaller particle size columns</li>
<li>HILIC as replacement for DCM/MeOH</li>
<li>Reversed phase</li>
</ul>
<h5>Who should attend this webinar mini-series?</h5>
<ul>
<li>Small Molecule Organic Chemists</li>
<li>Medicinal Chemists</li>
<li>And their Managers</li>
</ul>
<blockquote>
Presenter: Bob Bickler (Senior Technical Specialist, Biotage)
</blockquote>
<blockquote>
Bob Bickler is the Senior Technical Specialist at Biotage and has over 45 years of chromatography experience. During his career, he has worked for Whatman, YMC, Waters, and for the past 25 years at Biotage. Bob has designed and launched many products including Biotage SNAP columns, SP1, Isolera LS, Isolera Prime Flash System, as well as several accessories.
</blockquote>

Biotage: Improve your Flash Chromatography Skills: Tips for Sustainable Purifications

Improve your Flash Chromatography Skills: Tips for Sustainable Purifications

Attendees will see how currently available technologies can provide major solvent (and time) savings and improve their workflow and productivity.

Better Flash Chromatography Skills: Tips for Purifications

<h4>Overview</h4>A comprehensive webinar on flash chromatography. The fundamentals of chromatography theory, Method development of Normal phase, TLC to Flash and diving into the specifics of reversed-phase flash materials. Exploring the power of HILIC mode for polar compounds and learn how to efficiently transfer your HPLC methods to flash chromatography for preparative purification. This webinar will equip you with practical knowledge to enhance your purification workflows.<h4>Key Learning Points</h4><ul><li>Learn about general chromatography theory</li><li>Flash Chromatography fundamentals</li><li>Method Development approach-TLC, Normal phase and Reverse phase&nbsp;</li><li>HPLC to Flash Chromatography - Know how it works.</li></ul><h4>Who Should Attend</h4><ul><li>Researchers and Scientists: Ideal for those involved in the development and characterization of biotherapeutics.</li></ul><blockquote>Speaker: Dhanunjaya Naidu (Business Development Manager, Phenomenex)Dhanunjaya Naidu has earned a master’s degree in Organic Chemistry from Andhra University, India and brings 19 years of experience in synthesis, analytical method development and Purifications. In the past 7 years at Phenomenex, he has given many technical presentations, Analytical method development support, Column positioning and troubleshooting assistance.Currently, he is working as a Business Development Manager at Phenomenex India, and responsible for Pan India Purification Technologies Products. He has publications in reputed international journals in organic synthesis.</blockquote>

Phenomenex:  Enhancing Productivity Using Flash Chromatography: Strategies and Techniques

 Enhancing Productivity Using Flash Chromatography: Strategies and Techniques

Master flash chromatography fundamentals, method development, and HPLC-to-flash transfer to improve purification workflows for polar and complex compounds.

LCMS webinars focusing on Flash LC - page 1