News from LabRulezLCMS Library - Week 36, 2024

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 2nd September 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!

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This week we bring to you applications and other documents by Agilent Technologies, Shimadzu, Thermo Fisher Scientific, Waters Corporation, and Watrex!

1. Watrex: Watrex WATER PURIFICATION SYSTEMS

• Brochures and specifications

Systems:

• SCI-AQUA ROWAPUR - Reverse Osmosis Systems
• SCI-AQUA ULTRAPUR - Ultrapure Water
• SMART PUV - Ultrapure Water
• SMART PLUS N - Ultrapure Water

2. Shimadzu: On-Line Monitoring of Flow Synthesis Reactions Using Nexera FV

• Application

User Benefits

• On-line monitoring of flow synthesis reactions can be performed.
• Simple operation with dedicated software allows automatic execution of analysis at specific sampling intervals and automatic report generation.
• Reliable experimental system setup can be established using HPLC as a part of flow synthesis system.

Introduction: In the pharmaceutical and fine chemical industries, there is an increasing demand to switch from conventional batch manufacturing for the sake of improvement of efficiency (labor and manpower savings), quality and safety, and reduction of environmental load.

ICH-Q13 guidelines for continuous production of drug substances and drug products recommend the application of Process Analytical Technology (PAT) for the purpose of maintaining controlled conditions for manufacturing. Consequently, the demand for PAT is increasing.

Nexera FV is an HPLC system setup for on-line analysis equipped with a flow through vial in the autosampler (Fig. 1). The system enables automated processes from continuous delivery of reaction solution into the flow through vial to resulting report creation of HPLC analysis.

This article introduces an on-line monitoring of flow synthesis reactions using Nexera FV, taking an example of the esterification of carboxylic acid.

Conclusion: On-line monitoring of the esterification reaction of phenylpropionic acid by flow synthesis as performed using Nexera FV. Connecting the reaction system and the flow through vial, sampling and analysis was able to be performed automatically according to the analytical batch. In addition, the multi-data reporting function allowed reaction tracking on reactant and product by automatic generation of trend plot after analysis. Nexera FV and LabSolutions FV simplified analytical batch creation, automated sampling, and reporting. It results in a reduction of working time and increase of efficiency.

3. Agilent Technologies: SEC-MALS for mRNA Characterization with the Agilent 1260 Infinity II Multi-Angle Light Scattering Detector

• Application

Abstract: The characterization of messenger ribonucleic acid (mRNA) with mass spectrometry can be particularly challenging due to the complex and large structure of RNA molecules. The combination of multiangle light scattering (MALS) detection with size exclusion chromatography (SEC) can successfully be applied to the analysis of mRNA, delivering biophysical and structural information.

The Agilent 1260 Infinity II Multi-Angle Light Scattering Detector coupled to the Agilent 1290 Infinity II Bio LC System enables the determination of molecular weight (MW) in addition to aggregate percentage information from the concentration detector. The 1290 Infinity II Bio LC is specifically designed for conditions used in biochromatography, with a completely iron-free flow path and high tolerance to the salt-based buffers typically used in SEC.

Conclusion: An Agilent 1260 Infinity II Multi-Angle Light Scattering Detector coupled to an Agilent 1290 Infinity II Bio LC System provided biophysical information for the analysis of large mRNA molecules. The precise determination of the molecular weight was in near-perfect agreement with the theoretical value of the mRNA sequence. Good resolution was found between monomer and dimer of mRNA with an Agilent AdvanceBio SEC 1000Å column, leading to unique MW determination for the separated peaks.

The combination of the 1290 Infinity II Bio LC System and the 1260 Infinity II Multi-Angle Light Scattering Detector provides a complete biocompatible flow path to ensure trusted results for challenging solvent conditions or iron-reactive samples.

4. Waters Corporation: Leveraging Mobile Phase pH to Optimize Separations for Improved Prep Performance Using Columns Packed With Waters™ Hybrid Particles

• Application

Abstract

This application note illustrates the value of leveraging mobile phase pH to improve peak shape, retentivity, and mass loading for preparative separations. The Waters columns, manufactured with Hybrid Particle and Optimum Bed Density [(OBD™]) Technologies, enable the purification scientist to exploit the advantages of changing pH to improve chromatographic separations while maintaining consistent column-to-column performance, long column lifetimes, and direct scalability from analytical to prep. The OBD preparative columns resulted in better purification outcomes when compared to Vendor Y’s Column using the conditions specified in this study.

Benefits

• Changing the mobile phase pH can lead to enhanced performance with changes in retentivity and selectivity, improved peak shape, and increased mass loading, which save purification processing time.
• XBridge™ BEH™ C18 and XSelect™ CSH™ C18 Columns are stable over a wide pH range (1–12 and 1–11, respectively), effectively eliminating concerns about damaging the stationary phase due to low/high pH
  conditions within these ranges.
• Different C18 stationary phases provide additional selectivity choices (i.e., impacting the resolution and order of peak elution) for separating target compounds.
• The highly controlled OBD column packing process ensures preparative columns are of similar bed density to analytical columns of the same chemistry, thus making preparative chromatographic profiles analogous to analytical runs. Similar chromatography at the analytical and preparative scales simplifies scaleup and compound isolation.

5. Thermo Fisher Scientific: Accurate and consistent analysis of poly(A) tails of mRNA therapeutics on a UHPLC-HRAM-MS platform

• Application

Application benefits

• Accurate and consistent measurement of poly(A) tail length and distribution of in vitro transcribed (IVT) mRNA
• Automated and compliance-ready analysis and reporting of poly(A) tail lengths for IVT mRNA
• Seamless transfer of the IPRP-LC-HRAM-MS method from one LC-MS system to another via a Thermo Scientific™ Chromeleon™ CDS eWorkflow™ procedure

Goal

• Develop an automated and compliance-ready analytical solution for accurate and consistent analysis of poly(A) tails of IVT mRNA using an IPRP-UHPLC-HRAM-MS method from sample to report
• Execute the developed solution on three different LC-MS systems to demonstrate seamless transfer and data consistency

Keywords: Polyadenylated (poly(A)) tail analysis, intact mass determination, monoisotopic mass, liquid chromatography high-resolution accurate-mass mass spectrometry, ion-pairing reversed phase liquid chromatography, IPRP-LC, Vanquish Horizon UHPLC system, Orbitrap Exploris 240 mass spectrometer, Orbitrap Exploris MX mass detector, DNAPac reversed phase column, Chromeleon Chromatography Data System (CDS), high-resolution accurate mass, HRAM