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Watrex Praha
Watrex Praha
WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.
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Precision in Amino Acid Analysis

Th, 3.7.2025
| Original article from: Watrex Praha
The Sykam S 433 offers advanced amino acid analysis with ion exchange and HPLC precision, automated features, and expert support from Watrex Praha for routine and specialized labs.
<p><strong>Watrex: </strong>Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).</p>

Watrex: Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).

Amino acid analysis plays a crucial role in various scientific and industrial applications, from food quality control to clinical diagnostics and pharmaceutical research. Sykam, a leading manufacturer of analytical instruments, offers cutting-edge amino acid analyzers that combine the precision of ion exchange chromatography with the efficiency of modern high-performance liquid chromatography (HPLC). This article explores the features and benefits of Sykam’s flagship analyzer, the S 433, available through Watrex Praha.

Watrex: Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).Watrex: Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).

Amino Acid Analyzer S 433 

The S 433 is an innovative automatic amino acid analyzer designed to deliver unparalleled performance for routine and research applications. Its sophisticated design integrates several advanced features, making it a comprehensive solution for amino acid determination.

Key Features: 
  • Multistep Separation: Only two or three buffer solutions have to be combined to form the best optimized buffer profile at any part of the separation program. No more compromises by the limitation to four or five buffer changes. 
  • Cooled Reagent Storage: All buffers and the Ninhydrine reagent are stored under inert gas pressure in a refrigerated compartment, preventing oxidation and contamination. 
  • Integrated Autosampler: With a capacity of 120 vials and precise volume control (1 μL to 100 μL), the autosampler ensures minimal sample loss and eliminates cross-contamination through programmable washing. 
  • Enhanced Detection: The dual-channel photometer detects amino acids at 440 nm and 570 nm wavelengths, offering high sensitivity and reliability. 
  • Integrated Vacuum Degasser: Avoids the interruption of the buffer pump by air bubbles without the need of bubble traps with varying volumes, causing changes in retention times of the different amino acids. 
  • Separation Column Oven: An Integrated solid state column oven with fast heating and cooling capability with a temperature range from +20°C to +99°C independent of the ambient temperature. Up to twelve programmable temperature steps can be used during one separation program. 
  • High-Temperature Reactor: High-Temperature Reactor with a programmable temperature range from ambient to 180°C with a coiled capillary for the color reaction of the amino acid-ninhydrine complex. Automatic flushing of the reactor coil with a washing solution after each run prevents the blockage of the capillary.
  • User-Friendly Design: The system features integrated vacuum degassers, inert materials for all contact points, programmable reactors, and temperature-controlled columns to deliver consistent and reproducible results. 

The modular system design of the S 433 enables the budget-minded buyer to use several components as parts for a standard HPLC system without further costs.

Watrex: Fig 2: Flow Diagram S 433. (The original image is borrowed from Sykam).Watrex: Fig 2: Flow Diagram S 433. (The original image is borrowed from Sykam).

Applications 

The S 433 analyzer is suitable for a broad range of sample types, including: 

  • Protein hydrolysates 
  • Physiological fluids (serum, urine) 
  • Pharmacological samples

Key applications include detecting inborn metabolism errors, food and feedstuff analysis, biogenic amine profiling, and pharmaceutical quality control. 

Why Choose Sykam Analyzers from Watrex Praha? 

Sykam amino acid analyzers offer a unique combination of reliability, accuracy and flexibility. The innovative features of the S 433 ensure efficient and reproducible results, making it ideal for both routine and specialized applications. In addition, cooperation with Watrex Praha brings users expert support, ready-made solutions and flexibility to meet the demands of modern analytics. 

For more information contact Watrex Praha at [email protected].

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