LCMS
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike
Author
Watrex Praha
Watrex Praha
WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.
Tags
Article
Product
LinkedIn Logo

Precision in Amino Acid Analysis

Th, 3.7.2025
| Original article from: Watrex Praha
The Sykam S 433 offers advanced amino acid analysis with ion exchange and HPLC precision, automated features, and expert support from Watrex Praha for routine and specialized labs.
<p><strong>Watrex: </strong>Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).</p>

Watrex: Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).

Amino acid analysis plays a crucial role in various scientific and industrial applications, from food quality control to clinical diagnostics and pharmaceutical research. Sykam, a leading manufacturer of analytical instruments, offers cutting-edge amino acid analyzers that combine the precision of ion exchange chromatography with the efficiency of modern high-performance liquid chromatography (HPLC). This article explores the features and benefits of Sykam’s flagship analyzer, the S 433, available through Watrex Praha.

Watrex: Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).Watrex: Fig 1: Amino Acid Analyzer from Sykam. (The original image is borrowed from Sykam).

Amino Acid Analyzer S 433 

The S 433 is an innovative automatic amino acid analyzer designed to deliver unparalleled performance for routine and research applications. Its sophisticated design integrates several advanced features, making it a comprehensive solution for amino acid determination.

Key Features: 
  • Multistep Separation: Only two or three buffer solutions have to be combined to form the best optimized buffer profile at any part of the separation program. No more compromises by the limitation to four or five buffer changes. 
  • Cooled Reagent Storage: All buffers and the Ninhydrine reagent are stored under inert gas pressure in a refrigerated compartment, preventing oxidation and contamination. 
  • Integrated Autosampler: With a capacity of 120 vials and precise volume control (1 μL to 100 μL), the autosampler ensures minimal sample loss and eliminates cross-contamination through programmable washing. 
  • Enhanced Detection: The dual-channel photometer detects amino acids at 440 nm and 570 nm wavelengths, offering high sensitivity and reliability. 
  • Integrated Vacuum Degasser: Avoids the interruption of the buffer pump by air bubbles without the need of bubble traps with varying volumes, causing changes in retention times of the different amino acids. 
  • Separation Column Oven: An Integrated solid state column oven with fast heating and cooling capability with a temperature range from +20°C to +99°C independent of the ambient temperature. Up to twelve programmable temperature steps can be used during one separation program. 
  • High-Temperature Reactor: High-Temperature Reactor with a programmable temperature range from ambient to 180°C with a coiled capillary for the color reaction of the amino acid-ninhydrine complex. Automatic flushing of the reactor coil with a washing solution after each run prevents the blockage of the capillary.
  • User-Friendly Design: The system features integrated vacuum degassers, inert materials for all contact points, programmable reactors, and temperature-controlled columns to deliver consistent and reproducible results. 

The modular system design of the S 433 enables the budget-minded buyer to use several components as parts for a standard HPLC system without further costs.

Watrex: Fig 2: Flow Diagram S 433. (The original image is borrowed from Sykam).Watrex: Fig 2: Flow Diagram S 433. (The original image is borrowed from Sykam).

Applications 

The S 433 analyzer is suitable for a broad range of sample types, including: 

  • Protein hydrolysates 
  • Physiological fluids (serum, urine) 
  • Pharmacological samples

Key applications include detecting inborn metabolism errors, food and feedstuff analysis, biogenic amine profiling, and pharmaceutical quality control. 

Why Choose Sykam Analyzers from Watrex Praha? 

Sykam amino acid analyzers offer a unique combination of reliability, accuracy and flexibility. The innovative features of the S 433 ensure efficient and reproducible results, making it ideal for both routine and specialized applications. In addition, cooperation with Watrex Praha brings users expert support, ready-made solutions and flexibility to meet the demands of modern analytics. 

For more information contact Watrex Praha at [email protected].

Watrex Praha
LinkedIn Logo
 

Related content

Quantitation of 18 Per and Polyfluoroalkyl Substances (PFAS) in Shrimp with Agilent LC/TQ

Applications
| 2025 | Agilent Technologies
Instrumentation
Consumables, Sample Preparation, LC/MS, LC/QQQ, LC/MS/MS
Manufacturer
Agilent Technologies
Industries
Environmental

Configuring a combustion-ion chromatography system using a complete workflow

Technical notes
| 2025 | Thermo Fisher Scientific
Instrumentation
Ion chromatography
Manufacturer
Thermo Fisher Scientific
Industries
Environmental

Automatic Optimization of Gradient Conditions Using AI Algorithm for LC Method Development with Functional Foods

Posters
| 2025 | Shimadzu (HPLC Symposium)
Instrumentation
Software, HPLC
Manufacturer
Shimadzu
Industries
Food & Agriculture

Characterization of four saturated fatty acids using gradient HPLC-CAD highlighting optimized evaporation temperature control features

Posters
| 2025 | Thermo Fisher Scientific (HPLC Symposium)
Instrumentation
HPLC
Manufacturer
Thermo Fisher Scientific
Industries
Pharma & Biopharma

Single Quadrupole Mass Spectrometry Quick Workflows Specified to Oligonucleotides Screening

Posters
| 2025 | Shimadzu (ASMS)
Instrumentation
LC/MS, LC/SQ
Manufacturer
Shimadzu
Industries
Pharma & Biopharma
 

Related articles

Considerations when working with solid samples and SPE
Article | Academy

Considerations when working with solid samples and SPE

Solid phase extraction enables clean-up, pre-concentration, and solvent switching. Proper solvent choice and SPE mode are key for effective solid sample preparation.
Phenomenex
tag
share
more
EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders
Article | Environment

EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders

ALS is committed to climate action and sustainability, aiming for Net Zero by 2050. Through innovation and science, we strive to make a meaningful impact and share progress with clients.
ALS Czech Republic
tag
share
more
News from LabRulezLCMS Library - Week 29, 2025
Article | Poster

News from LabRulezLCMS Library - Week 29, 2025

This week we bring you posters by Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / ASMS and Waters Corporation / ASMS!
LabRulez
tag
share
more
Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?
Article | Product

Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?

Streamline™ OLE 200 by Watrex Praha automates solid-phase extraction for HPLC. Process up to 8 samples, reduce manual errors, and increase lab throughput with flexible, cartridge-based integration.
Watrex Praha
tag
share
more
 

Related content

Quantitation of 18 Per and Polyfluoroalkyl Substances (PFAS) in Shrimp with Agilent LC/TQ

Applications
| 2025 | Agilent Technologies
Instrumentation
Consumables, Sample Preparation, LC/MS, LC/QQQ, LC/MS/MS
Manufacturer
Agilent Technologies
Industries
Environmental

Configuring a combustion-ion chromatography system using a complete workflow

Technical notes
| 2025 | Thermo Fisher Scientific
Instrumentation
Ion chromatography
Manufacturer
Thermo Fisher Scientific
Industries
Environmental

Automatic Optimization of Gradient Conditions Using AI Algorithm for LC Method Development with Functional Foods

Posters
| 2025 | Shimadzu (HPLC Symposium)
Instrumentation
Software, HPLC
Manufacturer
Shimadzu
Industries
Food & Agriculture

Characterization of four saturated fatty acids using gradient HPLC-CAD highlighting optimized evaporation temperature control features

Posters
| 2025 | Thermo Fisher Scientific (HPLC Symposium)
Instrumentation
HPLC
Manufacturer
Thermo Fisher Scientific
Industries
Pharma & Biopharma

Single Quadrupole Mass Spectrometry Quick Workflows Specified to Oligonucleotides Screening

Posters
| 2025 | Shimadzu (ASMS)
Instrumentation
LC/MS, LC/SQ
Manufacturer
Shimadzu
Industries
Pharma & Biopharma
 

Related articles

Considerations when working with solid samples and SPE
Article | Academy

Considerations when working with solid samples and SPE

Solid phase extraction enables clean-up, pre-concentration, and solvent switching. Proper solvent choice and SPE mode are key for effective solid sample preparation.
Phenomenex
tag
share
more
EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders
Article | Environment

EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders

ALS is committed to climate action and sustainability, aiming for Net Zero by 2050. Through innovation and science, we strive to make a meaningful impact and share progress with clients.
ALS Czech Republic
tag
share
more
News from LabRulezLCMS Library - Week 29, 2025
Article | Poster

News from LabRulezLCMS Library - Week 29, 2025

This week we bring you posters by Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / ASMS and Waters Corporation / ASMS!
LabRulez
tag
share
more
Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?
Article | Product

Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?

Streamline™ OLE 200 by Watrex Praha automates solid-phase extraction for HPLC. Process up to 8 samples, reduce manual errors, and increase lab throughput with flexible, cartridge-based integration.
Watrex Praha
tag
share
more
 

Related content

Quantitation of 18 Per and Polyfluoroalkyl Substances (PFAS) in Shrimp with Agilent LC/TQ

Applications
| 2025 | Agilent Technologies
Instrumentation
Consumables, Sample Preparation, LC/MS, LC/QQQ, LC/MS/MS
Manufacturer
Agilent Technologies
Industries
Environmental

Configuring a combustion-ion chromatography system using a complete workflow

Technical notes
| 2025 | Thermo Fisher Scientific
Instrumentation
Ion chromatography
Manufacturer
Thermo Fisher Scientific
Industries
Environmental

Automatic Optimization of Gradient Conditions Using AI Algorithm for LC Method Development with Functional Foods

Posters
| 2025 | Shimadzu (HPLC Symposium)
Instrumentation
Software, HPLC
Manufacturer
Shimadzu
Industries
Food & Agriculture

Characterization of four saturated fatty acids using gradient HPLC-CAD highlighting optimized evaporation temperature control features

Posters
| 2025 | Thermo Fisher Scientific (HPLC Symposium)
Instrumentation
HPLC
Manufacturer
Thermo Fisher Scientific
Industries
Pharma & Biopharma

Single Quadrupole Mass Spectrometry Quick Workflows Specified to Oligonucleotides Screening

Posters
| 2025 | Shimadzu (ASMS)
Instrumentation
LC/MS, LC/SQ
Manufacturer
Shimadzu
Industries
Pharma & Biopharma
 

Related articles

Considerations when working with solid samples and SPE
Article | Academy

Considerations when working with solid samples and SPE

Solid phase extraction enables clean-up, pre-concentration, and solvent switching. Proper solvent choice and SPE mode are key for effective solid sample preparation.
Phenomenex
tag
share
more
EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders
Article | Environment

EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders

ALS is committed to climate action and sustainability, aiming for Net Zero by 2050. Through innovation and science, we strive to make a meaningful impact and share progress with clients.
ALS Czech Republic
tag
share
more
News from LabRulezLCMS Library - Week 29, 2025
Article | Poster

News from LabRulezLCMS Library - Week 29, 2025

This week we bring you posters by Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / ASMS and Waters Corporation / ASMS!
LabRulez
tag
share
more
Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?
Article | Product

Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?

Streamline™ OLE 200 by Watrex Praha automates solid-phase extraction for HPLC. Process up to 8 samples, reduce manual errors, and increase lab throughput with flexible, cartridge-based integration.
Watrex Praha
tag
share
more
 

Related content

Quantitation of 18 Per and Polyfluoroalkyl Substances (PFAS) in Shrimp with Agilent LC/TQ

Applications
| 2025 | Agilent Technologies
Instrumentation
Consumables, Sample Preparation, LC/MS, LC/QQQ, LC/MS/MS
Manufacturer
Agilent Technologies
Industries
Environmental

Configuring a combustion-ion chromatography system using a complete workflow

Technical notes
| 2025 | Thermo Fisher Scientific
Instrumentation
Ion chromatography
Manufacturer
Thermo Fisher Scientific
Industries
Environmental

Automatic Optimization of Gradient Conditions Using AI Algorithm for LC Method Development with Functional Foods

Posters
| 2025 | Shimadzu (HPLC Symposium)
Instrumentation
Software, HPLC
Manufacturer
Shimadzu
Industries
Food & Agriculture

Characterization of four saturated fatty acids using gradient HPLC-CAD highlighting optimized evaporation temperature control features

Posters
| 2025 | Thermo Fisher Scientific (HPLC Symposium)
Instrumentation
HPLC
Manufacturer
Thermo Fisher Scientific
Industries
Pharma & Biopharma

Single Quadrupole Mass Spectrometry Quick Workflows Specified to Oligonucleotides Screening

Posters
| 2025 | Shimadzu (ASMS)
Instrumentation
LC/MS, LC/SQ
Manufacturer
Shimadzu
Industries
Pharma & Biopharma
 

Related articles

Considerations when working with solid samples and SPE
Article | Academy

Considerations when working with solid samples and SPE

Solid phase extraction enables clean-up, pre-concentration, and solvent switching. Proper solvent choice and SPE mode are key for effective solid sample preparation.
Phenomenex
tag
share
more
EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders
Article | Environment

EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders

ALS is committed to climate action and sustainability, aiming for Net Zero by 2050. Through innovation and science, we strive to make a meaningful impact and share progress with clients.
ALS Czech Republic
tag
share
more
News from LabRulezLCMS Library - Week 29, 2025
Article | Poster

News from LabRulezLCMS Library - Week 29, 2025

This week we bring you posters by Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / ASMS and Waters Corporation / ASMS!
LabRulez
tag
share
more
Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?
Article | Product

Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC?

Streamline™ OLE 200 by Watrex Praha automates solid-phase extraction for HPLC. Process up to 8 samples, reduce manual errors, and increase lab throughput with flexible, cartridge-based integration.
Watrex Praha
tag
share
more
Other projects
GCMS
ICPMS
Follow us
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike