A novel approach for optimizing online comprehensive LC × LC methods, addressing the challenges posed by second-dimension injection band broadening under sample solvent mismatch or volume overload.

The researchers demonstrated the use of a newly developed 2.5 µm column with superior kinetic efficiency, reduced run times, and improved resolution for the analysis of Adeno Associated Viruses (AAVs).

The researchers used artificial neural networks to advance a fundamental understanding of retention interactions in supercritical fluid chromatography.

In the in Trends in Analytical Chemistry journal the researchers from the Institute of Physiology of the Czech Academy of Sciences, gave comprehensive overview of metabolomics and lipidomics tools.

In the in ACS Analytical Chemistry the researchers took a deep dive into understanding the retention mechanism of oligonucleotides, and evaluation of the linear solvent strength (LSS) retention model.

The study addresses the complexity of plant extract analysis by developing a holistic two-injection method that integrates UHPSFC with multimodal ionization MS/MS.

The traditional method of High Performance LC (HPLC) was compared with the currently increasingly used Ultra- High Performance LC (UHPLC) on a representative group of 11 hop samples.

The Ultra High Performance Liquid Chromatography (UHPLC), which uses the principle of separation on sub-2 μm particles, was tested for the separation and determination of steric forms of iso-α-acids in beer.

The advantages of UHPLC over the traditional HPLC are a nine-fold reduction of analysis time, double improvement of resolution, and triple improvement of sensitivity.