Learn how to balance purity, yield, and throughput when scaling analytical HPLC methods to preparative chromatography.

Discover how hybrid HPLC materials extend pH stability up to 12, improving retention and peak shape for basic compounds. Learn how Gemini™ and Kinetex™ EVO columns enhance LC-MS/MS performance.

Learn how to minimize peak tailing when analyzing basic compounds on type A silicas. Explore pH adjustment, mobile phase modifiers, and modern type B silica columns for sharper, more symmetrical peaks.

Learn how silica end-capping improves HPLC column performance. Discover how C18, PS-C18, Polar C18, and XB-C18 chemistries enhance retention, peak shape, and selectivity for various analytes.

Discover how bio-inert HPLC hardware enhances oligonucleotide analysis. Learn how BioTi™ columns improve resolution, sensitivity, and reduce adduct formation compared to stainless steel.

Learn how the choice and concentration of alkylamine ion-pair agents affect oligonucleotide retention and detection in HPLC. Explore method optimization with Phenomenex Biozen™ Oligo columns.

Learn how to develop robust HPLC methods for peptide analysis using systematic screening of column chemistry, pH, and gradients for optimal selectivity and reproducibility.

Discover practical tips to optimise LC systems for UHPLC performance on any HPLC. Learn how to reduce peak broadening, improve efficiency, and shorten runtimes.

After SPE, samples may not be ready for direct LC injection. Solvent mismatch with the mobile phase can distort peak shapes—proper solvent choice is essential.

Practical troubleshooting guide for low recovery in SPE methods. Learn how to identify problems with solvents, flow rate, pH, or sorbent choice step by step.