Column contamination is a leading cause of shortened HPLC column lifetime, leading to high back pressure. Learn why it happens and how to prevent blocked frits with simple precautions.

Over-drying silica-based SPE cartridges during conditioning can reduce analyte retention and recovery. Learn best practices to avoid losses and ensure reproducible results.

Polysaccharide chiral stationary phases enable wide enantiomeric recognition, solvent flexibility, and robust resolution with coated or immobilised designs.

Chiral separations require careful screening of stationary phases. Learn how a systematic approach with polysaccharide-based columns streamlines method development.

Best practices for sample preparation in peptide mapping with trypsin, covering denaturation, alkylation, buffer exchange, and protease selection for complete sequence coverage.

C18 columns with residual positive charge improve peak shape of basic analytes at low pH by reducing silanol interactions and enabling 100% aqueous mobile phases.

Bioanalytical samples often need pretreatment before SPE. Each matrix presents challenges like protein removal, red blood cell lysis, glucuronide hydrolysis, or tissue homogenization.

Struggling with peak tailing of basic compounds in RP-LC using type A silica columns? Discover how low-pH buffers and silanol suppressors can improve peak shape—plus why type B silica is a better choice.

Solid phase extraction enables clean-up, pre-concentration, and solvent switching. Proper solvent choice and SPE mode are key for effective solid sample preparation.

New end-capping technologies can significantly improve the analysis of compounds that were once considered challenging.