The upgrade to the Clarity version 9.0 version is paid, a new user code is needed for this version. Customers who purchased their Clarity software after July 25th, 2022 are eligible for a free-of-charge upgrade under the condition they have <a href="https://www.dataapex.com/swregistration?set_sn=1">registered their software</a>. Please refer to the <a href="https://www.dataapex.com/product/upgrade">upgrade details</a>.
<blockquote>
💡 <a href="https://www.dataapex.com/product/clarity-demo">Download our free Clarity Demo version</a>
</blockquote>
<blockquote>
💡 A detailed list of changes is available in <a href="https://www.dataapex.com/version_history?version=9.0">whatsnew</a>.
</blockquote>
<h3>What is new in Clarity 9.0?</h3>
<ul>
<li>Improvements of the Integration Algorithm – new algorithm Wave based on previous Experimental</li>
<li>Batch dialog redesign, improved handling of calibration during reprocessing</li>
<li>Improvements in Audit Trail</li>
<li>Clarity design adjusted to the Windows 11 environment</li>
<li>A new option Move down after Enter in Sequence Table to support barcode readers</li>
<li>Updated SW upgrade policy including paid upgrades of major versions</li>
<li>New and updated control modules</li>
</ul>
<h3>Batch Dialog</h3>
<ul>
<li>Options regrouped to reflect the real workflow</li>
<li>Calibration cloning from Batch issue fixed</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Batch_Dialog_in_new_in_Clarity_9_0_cf2d812841/DataApex-Batch-Dialog-in-new-in-Clarity-9.0.jpg" alt="DataApex: Batch Dialog in new in Clarity 9.0"/>
<h3>Audit Trail</h3>
<ul>
<li>Logging of system messages and pop-up windows improved</li>
<li>It now contains info about the instrument and user</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Audit_Trail_in_new_in_Clarity_9_0_c36d04f379/DataApex-Audit-Trail-in-new-in-Clarity-9.0.jpg" alt="DataApex: Audit Trail in new in Clarity 9.0"/>
<h3>Integration algorithm - WAVE</h3>
<ul>
<li>New Wave Integration Algorithm based on previous Experimental algorithm
<ul>
<li>In most cases, integration using just Global Parameters and Integration Interval works better</li>
</ul>
</li>
<li>Selection of Integration Algorithm moved to the Integration tab
</li>
</ul>
<blockquote>
For more details refer to <a href="https://www.dataapex.com/download/manuals">Clarity 9.0 vs 8.8 manual</a>
</blockquote>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Integration_algorithm_WAVE_in_new_in_Clarity_9_0_c72146007a/DataApex-Integration-algorithm-WAVE-in-new-in-Clarity-9.0.jpg" alt="DataApex: Integration algorithm - WAVE in new in Clarity 9.0"/>
<h3>Barcode reader support in sequence table</h3>
<ul>
<li>The Sequence Table - On Enter option defines where the focus moves in the Sequence table after pressing Enter:<ul>
<li>Move Right – default option</li>
<li>Move Down – used for barcode readers emulating keyboard</li>
</ul>
</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Barcode_reader_support_in_sequence_table_in_new_in_Clarity_9_0_e52f5e99c8/DataApex-Barcode-reader-support-in-sequence-table-in-new-in-Clarity-9.0.jpg" alt="DataApex: Barcode reader support in sequence table in new in Clarity 9.0"/>
<h3>Updated SW upgrade policy</h3>
<ul>
<li>New software license comes with free upgrades one-year period
</li>
<li>Major versions – upgrade
<ul>
<li>Change of the version number is before the dot - A.b.c.d</li>
<li>Includes the change of the user code</li>
<li>Are paid (unless the user is entitled to one-year free upgrade)</li>
</ul>
</li>
<li>Minor versions – update
<ul>
<li>Change of the version number is after the first dot - a.B.C.D</li>
<li>Free of charge</li>
</ul>
</li>
<li>We sell and provide support only for the latest version of Clarity
</li>
</ul>
<blockquote>
For more details refer to <a href="https://www.dataapex.com/product/upgrade">Clarity SW upgrade</a>
</blockquote>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_9_Advanced_Chromatography_Software_1ab81db902/DataApex-Clarity-9-Advanced-Chromatography-Software.jpg" alt="DataApex: Clarity 9 Advanced Chromatography Software"/>
<h3>Other improvements and fixed bugs</h3>
<ul>
<li>Fixed MS Search and NIST libraries result differences caused by sending the wrong spectral range from Clarity to NIST
</li>
<li>Improvements in the behavior of Fraction Collectors
</li>
<li>Better handling of method version to prevent memory exhaustion
</li>
<li>New warning when method size exceeds the limit
</li>
</ul>
… and a lot more
<h3>New control modules</h3>
<ul>
<li>HTA 2000H, 2000HT, 2100H</li>
<li>QuadroCore MCore </li>
<li>Shanghai Sunny Hengping Scientific Instrument GC-MS</li>
</ul>
... and many control modules have been updated and improved, for more see <a href="https://www.dataapex.com/version_history">Changes in version 9.0.0 (25.07.2023)</a>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Shanghai_Sunny_Hengping_Scientific_Instrument_GC_1290_MSQ_8100_456d4423c1/Shanghai-Sunny-Hengping-Scientific-Instrument-GC1290-+-MSQ8100.jpg" alt="DataApex: Shanghai Sunny Hengping Scientific Instrument GC1290 + MSQ8100"/>

DataApex Clarity 9.0 Chromatography Software  Released

<h3>Introduction</h3>
Besides its use in analytical GC, HPLC and CE, the Clarity chromatography software is well suited also for control of preparative chromatography systems. Using the FCGP (General Purpose Fraction Collector) control module, many different fraction collectors could be controlled by the simple Next and Collect/Waste commands generated by the module and transferred to the devices either through the digital Outputs/Inputs or by suitable communication line (RS232, LAN, GSIOC). Not only dedicated fraction collectors, but also multi-position valves could be controlled. The fraction collection parameters (including time windows and signal level or slope triggered collection) are defined within the Clarity FC-GP method setup. 
Two examples of automated systems used for isolation and purification of antibodies, based on common instrumentation and controlled by Clarity chromatography software are presented.
<h3>Automated Immunoaffinity Chromatography</h3>
This instrument setup is designed to isolate specific antibodies from clarified serum. The serum is obtained from an animal immunized with the antigen which we want to generate antibodies against. The media in the column has the same or a similar antigen covalently bound to it, in order to capture antibodies which are specific to that antigen. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_1_Diagram_of_the_immunoaffinity_separation_system_2453e733ba/DataApex-Fig.-1-Diagram-of-the-immunoaffinity-separation-system.jpg" alt="DataApex: Fig. 1 – Diagram of the immunoaffinity separation system"/>
The instrument is configured around our existing lab infrastructure (benches, racks, shelves, rods for mounting columns, etc.). A 6 position selector valve allows selection of serum, a wash buffer, an elution buffer, or a column cleaning buffer. The unused positions are plugged so that they can be selected to put the instrument into a standby state which prevents any siphoning of the mobile phases due to gravity. 
The output of the selector valve leads to the pump. Immediately after the pump is a backpressure regulator to ensure proper seating of the check valves and a second one, serving as a pressure relief valve, as the pump does not feature pressure monitoring.
The tubing is then plumbed into a 6 port, 2 position valve, used to allow the direction of flow through the column to be reversed (see plumbing diagram). This allows strongly retained antibodies at the top of the column to be eluted quickly, with minimal exposure to the harsh elution buffer. After flowing through the column, the mobile phase returns to the valve and is directed through pH and UV detectors to a second 6 position selector valve, where the output can be directed to reservoirs for waste, eluted material, or material to be reloaded for further processing. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_2_Example_of_the_elution_cycle_in_immunoaffinity_separation_09c29ecf9a/DataApex-Fig.-2-Example-of-the-elution-cycle-in-immunoaffinity-separation.jpg" alt="DataApex: Fig. 2 - Example of the elution cycle in immunoaffinity separation"/>
Currently we have 13 instruments in service that match this general description. Each instrument takes up roughly 50 cm x 50 cm of bench space, making use of shelving above the bench to hold large reservoirs. The “prototype” for this instrument design was assembled primarily from components we had in our lab that were not in use.
The full version of Clarity with the LC module is used on all instruments in order to allow for expansion. Most of the stations already have the maximum number of 4 instruments installed. Serial communication ports for the valves and pumps are provided by a DataApex Multicom device. All valves are mounted on Valco Microelectric Actuators with RS-232 communication. Analog signals from the pH probe and UV absorbance detector are acquired using either an INT9 A/D PCI card, a 4 channel Colibrick device, or a 2 channel UPAD, depending on the instrument. The PCs used are from various manufacturers, or are built to specifications in house.
<h5>Table 1- Immunoafinity System Components</h5>
<h5>No. / Name / Description</h5>
<ol>
<li>Reservoirs / Sample, Wash, Eluent, Column Cleaning</li>
<li>Selection valve / 6 position selector valve, (Valco, C25-6186EMH)</li>
<li>Pump / SSI/LabAlliance Series 1 Pump (Chromtech, P-040), with 40 ml/min heads</li>
<li>Backpressure regulator / 40 psi, to provide backpressure for pump valves</li>
<li>Backpressure regulator / 100 psi, connected by a tee, serving as pressure relief valve</li>
<li>Switching valve / 6 port, 2 position valve (Valco C22-6186EH)</li>
<li>Column / 5 cm diameter x 5 cm length</li>
<li>pH detector / Flow cell (Sensorex, FC45C) with Flat pH electrode (Sensorex, S450CD)</li>
<li>UV detector / UV absorbance detector (Bio-Rad, EM-1)</li>
<li>Selection valve / 6 position selector valve, (Valco, C25-6186EMH)</li>
<li>Reservoirs / Waste, Pure Product, Impure Product for reloading</li>
</ol>
1/8” ID Dupont PFA tubing is used throughout for connections
Multiple isolation cycles are needed to process the starting material quantity. The sequence table is thus used to control the instrument. Separate methods are written for the loading, washing, elution, and cleaning phases of the process. These methods are then repeated in the sequence to create cycles, which can be performed automatically until the desired amount of material has been processed. Sequences can also be run overnight, with the primary limitation being the size of the mobile phase and collection reservoirs. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_3_Example_of_sequence_table_for_immunoaffinity_separation_58a994a3fe/DataApex-Fig.-3-Example-of-sequence-table-for-immunoaffinity-separation.jpg" alt="DataApex: Fig. 3 - Example of sequence table for immunoaffinity separation"/>
<h3>Preparative size exclusion chromatography with stacked injections using Clarity</h3>
This instrument setup is designed to facilitate removal of aggregates and other high molecular weight species from antibodies. Generally, the harshness of the elution buffer used in the affinity step leads to some degree of aggregation in the isolated antibodies.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_4_Diagram_of_the_Stacked_Injection_SEC_system_a7622a7986/DataApex-Fig.-4-Diagram-of-the-Stacked-Injection-SEC-system.jpg" alt="DataApex: Fig. 4 - Diagram of the Stacked Injection SEC system"/>
A 6 position selector valve is used to switch between a sample reservoir and a running buffer reservoir. This is connected to a pump, with backpressure regulator configured as a blow-off valve in order to protect the column from excessive pressure in the event of a clog. The outlet of the column is connected to a UV absorbance detector. Fractions are collected using a fraction collector. 
<h5>Table 2 - Size Exclusion System Components</h5>
<h5>No. / Name / Description</h5>
<ol>
<li>Reservoirs / Sample, Eluent, Column Cleaning</li>
<li>Selection valve / 6 position selector valve, (Valco, C25-6186EMH)</li>
<li>Pump / SSI/LabAlliance Series 1 Pump (Chromtech, P-040), with 40 ml/min heads</li>
<li>Backpressure regulator/ 40 psi, to provide backpressure for pump valves</li>
<li>Backpressure regulator / 100 psi, connected by a tee, serving as pressure relief valve</li>
<li>Column / 5 cm x 100 cm (GE, XK-50/100) column packed with Superdex 200 Prep Grade.</li>
<li>UV detector / UV absorbance detector (Bio-Rad, EM-1)</li>
<li>Fraction collector / Isco Foxy 200 fraction collector</li>
</ol>
1/8” ID Dupont PFA tubing is used throughout for connections.
Again, multiple injections are needed to load all the sample to be processed. In order to reduce the time required to process large samples, a stacked injection technique is used. As a run begins, the solvent selection valve loads the first portion of the sample from the sample reservoir then switches back to the running buffer.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_5_Example_of_Stacked_Injection_Method_Event_Table_687cda6e11/DataApex-Fig.-5-Example-of-Stacked-Injection-Method-Event-Table.jpg" alt="DataApex: Fig. 5 - Example of Stacked Injection Method Event Table"/>
After the first portion has migrated some distance down the column, the valve switches to the sample reservoir loading a second portion. After the second portion has migrated down the column a third can be loaded, and so on. If the characteristics of the sample are known, spacing between samples can be reduced significantly versus loading one sample and waiting for it to elute completely. In one example, a single sample takes 165 minutes to elute completely, however 8 samples can be processed in 790 minutes, saving almost 9 hours by using stacked injections. Multi-gram quantities of material can be processed per day using this technique. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_6_Example_of_stacked_injection_SEC_separation_019f37638a/DataApex-Fig.-6-Example-of-stacked-injection-SEC-separation.jpg" alt="DataApex: Fig. 6 - Example of stacked injection SEC separation"/>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Fig_7_Detail_of_stacked_injection_SEC_separation_cycle_bc1be53e3f/DataApex-Fig.-7-Detail-of-stacked-injection-SEC-separation-cycle.jpg" alt="DataApex: Fig. 7 - Detail of stacked injection SEC separation cycle"/>
<h3>Conclusion</h3>
We have demonstrated the use of Clarity in purification of antibodies using two specific setups, the automated immunoaffinity chromatography and preparative size exclusion chromatography with stacked injections.
Similar setups can be used with large range of fraction collectors and multi-position valves. The description can also help with setting up systems for other preparative applications.
<h3>Featured Clarity products</h3>
In both applications the same Clarity Chromatography Software system has been used:
<h5>Table 3 - Clarity Software Components (Item - p/n)</h5>
<ul>
<li>Clarity Single Instrument - C50</li>
<li>LC Control Module - A24</li>
<li>AD Converters- e.g. Colibrick 4 ch - U34</li>
<li>Add on instruments (optional – to control more systems from 1 station) - C55</li>
</ul>

Automated SW solution for preparative HPLC

Dear customers and business partners,
We are happy to announce our latest version of Clarity 9.1. This version introduces new control modules for Chromsystems, ECOM, and Welch. It also includes updated Advion, ECOM, Labio, NETEL, Sykam, YoungIn Chromass, and Agilent ICF control modules. A detailed list of changes is available in the What is new section on our web.
This update is available to existing <a href="https://gcms.labrulez.com/products/999">Clarity 9.0</a> users at no additional cost.
<blockquote>
<h4><a href="https://www.dataapex.com/download?product_filter=3&amp;utm_campaign=Clarity%209.1&amp;utm_medium=email&amp;utm_id=MKT1871&amp;utm_source=newsletter">DOWNLOAD CLARITY</a></h4>
</blockquote>
If you are using a version prior to 9.0, please review our upgrade policy. We recommend upgrading to the latest software version. By doing so, you will not only gain access to the newest features but also benefit from our expert technical support, which is provided for free with our latest software versions.
Your DataApex team
<h3>Tha latest literature on Clarity software from DataApex in <a href="https://gcms.labrulez.com/library?from=2023&amp;mainauthorItems=95&amp;search=Clarity&amp;sources=LCMS_EN-ICPMS_EN-GCMS_EN&amp;to=2024">LabRulez Library</a></h3>
<ul>
<li>REGULATED ENVIRONMENT IN REGARD TO CLARITY (Presentations | 2023)
</li>
<li>Clarity VALIDATION (Presentations | 2023)
</li>
<li>Clarity Software + Hardware VALIDATION KIT (Manuals | 2023)
</li>
<li>Why Clarity Chromatography Software? (Presentations | 2023)
</li>
<li>Clarity Software REFERENCE GUIDE (Manuals | 2023)
</li>
<li>Clarity SST – SYSTEM SUITABILITY TEST CLARITY EXTENSION (Presentations | 2023)
</li>
<li>Declaration of Software Validation - Structural validation and Statement of compliance with 21 CFR Part 11 (Others | 2023)
</li>
</ul>

DataApex Clarity 9.1 released

<h3>Promotion Alert!</h3>
For a limited time, customers who replace their existing chromatography software or integrator with <a href="https://lcms.labrulez.com/products/999">Clarity</a> or Clarity Lite Chromatography Software will receive a 20% discount on the software! This promotion is applicable also for old DataApex software (CSW17/CSW32). The promotion is valid through August 31, 2024.
Please contact <a href="https://www.dataapex.com/">DataApex</a> or your <a href="https://lcms.labrulez.com/products/999">distributor</a> for more details.
<h4>Why migrate to Clarity/Clarity Lite?</h4>
<ul>
<li>User-friendly interface.</li>
<li>Scalable solution.</li>
<li>Comprehensive data analysis tools.</li>
<li>Wide range of over 1000 controlled instruments.</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Replace_your_current_Chromatography_Software_with_Clarity_and_Save_Big_190b40fbee.jpg" alt="DataApex: Replace your current Chromatography Software with Clarity and Save Big!"/>

Replace your current Chromatography Software with Clarity and Save Big!

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 2nd September 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote><h4>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></h4></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring to you applications and other documents by Agilent Technologies, Shimadzu, Thermo Fisher Scientific, Waters Corporation, and Watrex!<h3>1. <style type="text/css"></style>Watrex: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_Water_Catalog_3a41a1462e.pdf">Watrex WATER PURIFICATION SYSTEMS</a></h3><ul><li>Brochures and specifications</li></ul>Systems:<ul><li>SCI-AQUA ROWAPUR - Reverse Osmosis Systems</li><li>SCI-AQUA ULTRAPUR - Ultrapure Water</li><li>SMART PUV - Ultrapure Water</li><li>SMART PLUS N - Ultrapure Water</li></ul><h3>2. <style type="text/css"></style>Shimadzu: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00779_en_063e0bfcd8.pdf">On-Line Monitoring of Flow Synthesis Reactions Using Nexera FV</a></h3><ul><li>Application</li></ul>User Benefits<ul><li>On-line monitoring of flow synthesis reactions can be performed.</li><li>Simple operation with dedicated software allows automatic execution of analysis at specific sampling intervals and automatic report generation.</li><li>Reliable experimental system setup can be established using HPLC as a part of flow synthesis system.</li></ul>Introduction: In the pharmaceutical and fine chemical industries, there is an increasing demand to switch from conventional batch manufacturing for the sake of improvement of efficiency (labor and manpower savings), quality and safety, and reduction of environmental load.ICH-Q13 guidelines for continuous production of drug substances and drug products recommend the application of Process Analytical Technology (PAT) for the purpose of maintaining controlled conditions for manufacturing. Consequently, the demand for PAT is increasing.Nexera FV is an HPLC system setup for on-line analysis equipped with a flow through vial in the autosampler (Fig. 1). The system enables automated processes from continuous delivery of reaction solution into the flow through vial to resulting report creation of HPLC analysis.This article introduces an on-line monitoring of flow synthesis reactions using Nexera FV, taking an example of the esterification of carboxylic acid.Conclusion: On-line monitoring of the esterification reaction of phenylpropionic acid by flow synthesis as performed using Nexera FV. Connecting the reaction system and the flow through vial, sampling and analysis was able to be performed automatically according to the analytical batch. In addition, the multi-data reporting function allowed reaction tracking on reactant and product by automatic generation of trend plot after analysis. Nexera FV and LabSolutions FV simplified analytical batch creation, automated sampling, and reporting. It results in a reduction of working time and increase of efficiency.<h3>3. <style type="text/css"></style>Agilent Technologies<style type="text/css"></style>: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_light_scattering_detector_1290_infinity_ii_bio_lc_5994_7745en_agilent_5c2dfb059e.pdf">SEC-MALS for mRNA Characterization with the Agilent 1260 Infinity II Multi-Angle Light Scattering Detector</a></h3><ul><li>Application</li></ul>Abstract: The characterization of messenger ribonucleic acid (mRNA) with mass spectrometry can be particularly challenging due to the complex and large structure of RNA molecules. The combination of multiangle light scattering (MALS) detection with size exclusion chromatography (SEC) can successfully be applied to the analysis of mRNA, delivering biophysical and structural information.The Agilent 1260 Infinity II Multi-Angle Light Scattering Detector coupled to the Agilent 1290 Infinity II Bio LC System enables the determination of molecular weight (MW) in addition to aggregate percentage information from the concentration detector. The 1290 Infinity II Bio LC is specifically designed for conditions used in biochromatography, with a completely iron-free flow path and high tolerance to the salt-based buffers typically used in SEC.Conclusion: An Agilent 1260 Infinity II Multi-Angle Light Scattering Detector coupled to an Agilent 1290 Infinity II Bio LC System provided biophysical information for the analysis of large mRNA molecules. The precise determination of the molecular weight was in near-perfect agreement with the theoretical value of the mRNA sequence. Good resolution was found between monomer and dimer of mRNA with an Agilent AdvanceBio SEC 1000Å column, leading to unique MW determination for the separated peaks.The combination of the 1290 Infinity II Bio LC System and the 1260 Infinity II Multi-Angle Light Scattering Detector provides a complete biocompatible flow path to ensure trusted results for challenging solvent conditions or iron-reactive samples.<h3>4. <style type="text/css"></style>Waters Corporation<style type="text/css"></style>: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008431_en_06ca2bef35.pdf">Leveraging Mobile Phase pH to Optimize Separations for Improved Prep Performance Using Columns Packed With Waters™ Hybrid Particles</a></h3><ul><li>Application</li></ul>AbstractThis application note illustrates the value of leveraging mobile phase pH to improve peak shape, retentivity, and mass loading for preparative separations. The Waters columns, manufactured with Hybrid Particle and Optimum Bed Density [(OBD™]) Technologies, enable the purification scientist to exploit the advantages of changing pH to improve chromatographic separations while maintaining consistent column-to-column performance, long column lifetimes, and direct scalability from analytical to prep. The OBD preparative columns resulted in better purification outcomes when compared to Vendor Y’s Column using the conditions specified in this study.Benefits<ul><li>Changing the mobile phase pH can lead to enhanced performance with changes in retentivity and selectivity, improved peak shape, and increased mass loading, which save purification processing time.</li><li>XBridge™ BEH™ C18 and XSelect™ CSH™ C18 Columns are stable over a wide pH range (1–12 and 1–11, respectively), effectively eliminating concerns about damaging the stationary phase due to low/high pH conditions within these ranges.</li><li>Different C18 stationary phases provide additional selectivity choices (i.e., impacting the resolution and order of peak elution) for separating target compounds.</li><li>The highly controlled OBD column packing process ensures preparative columns are of similar bed density to analytical columns of the same chemistry, thus making preparative chromatographic profiles analogous to analytical runs. Similar chromatography at the analytical and preparative scales simplifies scaleup and compound isolation.</li></ul><h3>5. <style type="text/css"></style>Thermo Fisher Scientific<style type="text/css"></style>: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ab_003060_pb_poly_a_uhplc_hram_ms_mrna_ab003060_na_en_f1ac90bd6f.pdf">Accurate and consistent analysis of poly(A) tails of mRNA therapeutics on a UHPLC-HRAM-MS platform</a></h3><ul><li>Application</li></ul>Application benefits<ul><li>Accurate and consistent measurement of poly(A) tail length and distribution of in vitro transcribed (IVT) mRNA</li><li>Automated and compliance-ready analysis and reporting of poly(A) tail lengths for IVT mRNA</li><li>Seamless transfer of the IPRP-LC-HRAM-MS method from one LC-MS system to another via a Thermo Scientific™ Chromeleon™ CDS eWorkflow™ procedure</li></ul>Goal<ul><li>Develop an automated and compliance-ready analytical solution for accurate and consistent analysis of poly(A) tails of IVT mRNA using an IPRP-UHPLC-HRAM-MS method from sample to report</li><li>Execute the developed solution on three different LC-MS systems to demonstrate seamless transfer and data consistency</li></ul>Keywords: Polyadenylated (poly(A)) tail analysis, intact mass determination, monoisotopic mass, liquid chromatography high-resolution accurate-mass mass spectrometry, ion-pairing reversed phase liquid chromatography, IPRP-LC, Vanquish Horizon UHPLC system, Orbitrap Exploris 240 mass spectrometer, Orbitrap Exploris MX mass detector, DNAPac reversed phase column, Chromeleon Chromatography Data System (CDS), high-resolution accurate mass, HRAM

News from LabRulezLCMS Library - Week 36, 2024

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 28th October 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you applications, technical notes, and brochures by Watrex Praha, Thermo Fisher Scientific, Shimadzu, Waters Corporation, and Agilent Technologies!<h4>1. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/59">Watrex Prague</a>: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Watrex_column_catalog_online_e63db33f5b.pdf">Watrex HPLC COLUMNS</a></h4><ul><li>Brochures and specifications</li></ul><h5>Ion chromatography - Analysis of cations</h5><ul><li>Watrex IonPlus™ CS19 - Styrene-divinylbenzene, 55% cross-linking (7 μm particle i.d.)</li></ul><h5>Ion chromatography - Analysis of anions</h5><ul><li>Watrex IonPlus™ AS19 - Styrene-divinylbenzene, 55% cross-linking (12 μm particle i.d.).</li><li>Watrex IC Anion I - Styrene-divinylbenzene (7 μm particle i.d.)</li></ul><h5>Reversed phase chromatography</h5><ul><li>Watrex DeltaSil™ 100 C18 column is a standard reversed phase for wide field of applications in analysis of drugs, organic compounds, peptides and others. Stationary phase is based on synthetic 5 μm silica-gel with 100 Å pores and 15% carbon content. The column has a good peak symmetry and efficiency. Theoretical plate count value for non-polar compounds, such as naphthalene, reaches up to 90.000 theoretical plates per meter.</li></ul><h5>Carbohydrate analysis</h5><ul><li>Watrex Polymer IEX™ is the Europe’s leading sorbent for ion exclusion analysis of alcohols, carboxylic acids, sugars, and polysacharides.</li></ul><h5>Gel-permeation chromatography</h5><ul><li>Watrex DeltaGel ™ - Mixed-bed GPC columns for SEC chromatography in organic solvents.</li></ul><h4>2. Agilent Technologies: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/wp_advanced_sample_linking_hplc_systems_5994_7570_EN_agilent_82989c0dce.pdf">Agilent Advanced Sample Linking (Infinity III)</a></h4><ul><li>Technical note</li></ul>AbstractWith the release of the Agilent Infinity III LC series, the Agilent 1260 and 1290 Infinity III Multisampler can optionally be equipped with the Agilent InfinityLab Sample ID Reader. The integration of the LC system into a client-server environment allows the use of additional software, such as Agilent Sample Linking software and Agilent Sample Scheduler for OpenLab, enabling a complete workflow that links samples from any laboratory information management system (LIMS) to the vials and HPLC analysis results. This white paper highlights the benefits of the integrated analytical workflow. In this workflow, barcodes are used to track samples from the sample list to their vial position, and the presence of the correct sample is recognized without manually entering its position. This saves time and improves ease of use, with fewer errors over the complete workflow.ConclusionThis white paper describes a workflow that begins with an analytical sequence initiated by any LIMS, then links the barcodes of sample containers to the barcodes of target pre-barcoded vials. The samples are analyzed to get the final data results, and a report is transmitted back to LIMS. This closed-loop, error-free, and time-saving workflow is enabled by Agilent Sample Linking software together with the Agilent Sample Scheduler for OpenLab. Samples can be tracked through the complete workflow using barcodes to transfer them accurately from their original containers to a final barcoded vial, managed completely by the Sample Linking software. This saves time, enhances ease of use, and results in fewer errors over the complete workflow.<h4>3. Waters Corporation: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008468_en_03451ccb29.pdf">Improving USP Monograph Analysis Time Through Scaling by N on an HPLC System Using CORTECS™ Premier Columns</a></h4><ul><li>Application</li></ul>AbstractThe United States Pharmacopeia General Chapter &lt;621&gt; allows certain changes to monograph methods. Changes in column length and particle size are employed as part of modernization efforts from HPLC to UPLC™ instrumentation. However, some modernization activities can be performed without switching LC systems. For HPLC analyses, reducing particle size from 5 μm to 3.5 μm can improve throughput and run times, however those columns increase backpressure of the system. Additionally, depending on the monograph, modernization may not be possible due to available column configurations, and the guidelines set in General Chapter &lt;621&gt; for scaling by L/dp. Modernizing by plate count (N) is one path forward, especially if the column being modernized to takes advantage of highly efficient particles. This application note scales the USP impurities assay of zidovudine from the original 4.6 x 250 mm column using fully porous 5 μm particles to a 4.6 x 150 mm column using solid-core particles to improve both throughput and solvent usage.Benefits<ul><li>USP Monograph for zidovudine impurities analyzed on various columns</li><li>Vastly improved separation efficiency using CORTECS Premier 5 μm columns with original USP monograph column configuration</li><li>40% improvement in solvent usage and up to 60% improvement in analysis run time using CORTECS Premier 5 μm Column</li></ul>IntroductionMonograph methods have traditionally employed the use of 5 μm particle size columns due to pressure limitations of older HPLC instrumentation. In combination with the larger particle stationary phases, longer column dimensions were needed in order to achieve appropriate column efficiency. This leads to lengthy analyses with high volumes of solvent being consumed. Monograph methods can be modernized however, and the United States Pharmacopeia (USP) outlines two routes for modernization as outlined in USP General Chapter &lt;621&gt;.1 The first is by changing particle size and column length (L/dp) which can be done but may require the use of more modern LC technology like UPLC. The second route to modernization involves scaling a column based on the plate count (N) for the assay. Changes can be made to either the L/dp or N by -25% and up to 50%. Modernizing by N allows a method to be changed to any configuration as long as the plate count for the new conditions falls within the limits outlined in General Chapter &lt;621&gt;.For some assays, modernizing by N can be possible by using higher efficiency stationary phases, like solid-core particles. Solid-core particle columns, like CORTECS columns, have been shown in the past to boost separation efficiency due to particle morphology.2–4 By taking advantage of the solid-core particles, a shorter column may be used while still achieving acceptable plate counts. Thus, improvements to run time and solvent usage could be possible without changing particle size, therefore avoiding the potential increases to system pressure accompanied with smaller particle sizes. In this application note, the assay and impurities monographs for zidovudine were scaled from a 250 mm column down to a 150 mm column using the modernize by N approach. Using the new CORTECS Premier 5 μm columns results in a 40% decrease in solvent usage with up to a 60% reduction in analysis time per sample.ConclusionValidated monograph methods often employ HPLC systems with larger particle size columns due to pressure limitations of the system. Modernization of these monograph methods can be performed under USP General Chapter &lt;621&gt; using either the length to particle size ratio (L/dp) or Plate Count (N). In either case, the new column configuration must be within -25% to 50% of the original conditions. Once a new column is selected, changes to gradient profile and flow rate are calculated. This application note focused on the assay and impurity test for zidovudine, which calls for a 250 mm column in the original monograph.By using CORTECS Premier 5 μm Column the assay and impurities test for zidovudine was successfully scaled down to a 150 mm column while maintaining the particle size. This greatly improved sample throughput with a 40% reduction in run time and solvent usage. Further improvements to throughput were realized by increasing flow rate from 1.5 mL/min to 2.25 mL/min. The increased flow rate, while not acceptable under USP General Chapter &lt;621&gt;, is still able to be run due to the lower system pressure generated by the 150 mm column. A 60% reduction in run time is realized with the higher flow rate. CORTECS Premier 5 μm Columns offer highly efficient solid-core particles combined with MaxPeak Premier High Performance Surface Technology hardware.<h4>4. Shimadzu: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00750_en_bee79113e7.pdf">Analysis of Per- and Polyfluoroalkyl Substances (PFAS) using Triple Quadrupole Mass Spectrometer Part 1 - Fish Fillet</a></h4><ul><li>Application</li></ul>User Benefits<ul><li>The optimized procedure for pretreatment and LC-MS/MS analytical conditions enable accurate quantification of thirty major PFASs targeted by AOAC SMPR from 0.1 μg/kg.</li><li>The method allows the initiation of PFASs analysis in food</li></ul>IntroductionPer- and Polyfluoroalkyl Substances (PFASs) are a collective name for more than four thousand organofluorine compounds. Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) are representative compounds of PFAS. They are used in a wide range of applications, such as fire retardants, food packaging materials, and non-stick coatings, due to their water-repellent, oil-repellent, heat-resistant, and chemical-resistant properties. Due to their structural stability, PFAS widely remains in the environment. There are concerns about health risks caused by human ingestion of fish that have ingested seawater, river water, or feed contaminated with PFAS. Therefore, quantitative assessment of PFAS levels in fish should be important.This application news introduces a quantitative analysis of PFAS in tuna fillets with LC-MS/MS. Thirty principal PFASs targeted by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/292">AOAC INTERNATIONAL</a>, a North American organization that standardizes food testing methods and verifies analytical methods, were analyzed using newly created method, which was investigated from the pretreatment procedure then validated by recovery test. By reducing losses during the pretreatment and optimizing the analytical conditions, good recovery rates were obtained for all compounds.ConclusionThis application news described a quantitative analysis of thirty PFAS targeted by AOAC INTERNATIONAL in tuna fillet. The analysis was performed using a quadrupole mass spectrometer LCMS-8060NX equipped with ultra-high performance liquid chromatograph Nexera X3 UHPLC system. Shim-pack Scepter, which provides good separation and peak shape, was used as the column. A spiked recovery test were conducted, and all compounds showed recovery rate within 80-120% and repeatability below 20% at spiked concentrations of 0.1, 1, 5 μg/kg. In particular, recovery rates of PFOS, PFOA, PFNA, and PFHxS were within 95.5-108.4% at all spiked concentrations. Using optimized pretreatment and analytical methods, accurate quantitation is possible from 0.1 μg/kg.<h4>5. Thermo Fisher Scientific: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/AN_003215_Highly_sensitive_method_for_the_determination_of_12_nitrosamine_impurities_in_multiple_AR_Bs_the_class_of_sartan_drug_formulations_c2744b4c40.pdf">Highly sensitive method for the determination of 12 nitrosamine impurities in multiple ARBs, the class of sartan drug formulations</a></h4><ul><li>Application</li></ul>Application benefits<ul><li>Single method applicable for highly sensitive quantitation of 12 nitrosamines in three ARB drug substances.</li><li>A robust gradient method with significant chromatographic separation between nitrosamines and ARB drug substances.</li><li>Better retentivity and resolution of nitrosamines and APIs with comparatively shorter runtime, ideal for high throughput screening.</li></ul>GoalDevelopment of a sensitive and robust LC-MS/MS method to quantitate the following 12 nitrosamine impurities in three ARB drug substances: N-nitrosodimethylamine (NDMA), N-nitroso-diethylamine (NDEA), N-ethyl-N-nitroso-2-propanamine (NEIPA), N-nitroso-diisopropylamine (NDIPA), N-nitroso-di-n-propylamine (NDPA), N-nitroso-methylphenylamine (NMPA), N-nitroso-di-n-butylamine (NDBA), N-nitroso-N-methyl-4-aminobutyric acid (NMBA), N-nitrosomorpholine (NMOR), N-nitrosopyrrolidine (NPYR), N-nitrosomethylethylamine (NMEA), and N-nitrosopiperidine (NPIP).IntroductionAngiotensin receptor blockers (also called ARBs or angiotensin II inhibitors) are medicines that dilate blood vessels and are used in the treatment of high blood pressure (hypertension), heart failure, or kidney disease in people with diabetes.1 These ARBs, also known as sartan medicines, have a specific tetrazole ring-containing structure whose synthesis could potentially lead to the formation of nitrosamine impurities.2If the content of nitrosamine impurities is found to be greater than the acceptable limit, then regulatory authorities need to be informed, and that may ultimately lead to the drug substance product being recalled. There have been numerous drug recalls associated with nitrosamine impurity levels exceeding the allowable limits in drug substances of various pharmacological categories, including ARBs.3 In 2018 and in 2019, the U.S. Food and Drug Administration (FDA) found traces of NDMA and NDEA impurities in the ARB drug products valsartan, losartan, and irbesartan. Since July 2018, more than two dozen specific ARB products have been recalled owing to the unacceptable presence of potentially carcinogenic nitrosamine impurities.4In 2018, the FDA issued guidance to the industry on how to assess and control impurities. EMA finalized a review under Article 5(3) of Regulation (EC) No 726/2004 in June 2020 to provide guidance to marketing authorization holders on how to avoid the presence of nitrosamine impurities in human medicines.The CHMP asked marketing authorization holders to review all chemical and biological human medicines for the possible presence of nitrosamines and test products at risk. Regulators across the globe have been vigilant on nitrosamine testing and subsequent modification of guidelines or testing procedures to ensure the best quality products reach the market. Recently, the FDA5 and EMEA6 updated guidelines with a more comprehensive and categorized approach towards nitrosamine testing.In this application note, the goal was to develop an LC-MS/MS solution to the increased frequency of demands for higher sensitivity and quantification of an extensive array of nitrosamine impurities in pharmaceutical formulations with a one-method approach for multiple products. From the multiple techniques available today, liquid chromatography-tandem mass spectrometry is one of the best for highly sensitive and reproducible quantification of nitrosamines. It not only benefits sensitivity in sub-nanogram concentrations but also provides a more robust, linear, and reproducible solution for nitrosamine detection. The guidelines followed in this application are from the ICH Q2(R2)7, FDA5, and EMEA6.As demonstrated by the excellent results observed in this study, the Thermo Scientific™ TSQ Altis™ Plus Triple Quadrupole mass spectrometer connected to the Thermo Scientific™ Vanquish™ Flex UHPLC system makes a suitable configuration for the highly sensitive analysis of multiple nitrosamines in a single method applicable for 3 ARBs—olmesartan, irbesartan, and valsartan. For data processing and reporting, Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) provides a complete solution, which fulfils all compliance requirements and ensures data integrity and security requirements are met, with a detailed workflow that includes user management along with instrument and data audit trails.

News from LabRulezLCMS Library - Week 44, 2024

<h4>Here's what Clarity 10.0 offers:</h4>For a comprehensive function overview, please refer to the full details on our website. Also, check our Upgrade policy.&nbsp;<ul style="margin-top:0px;"><li>Dual Chromatogram Mode: two samples can be measured during one analysis using dual tower or dual loop samplers.</li><li>Extended GLP options for regulated environments.</li><li>Many improvements for Clarity Extensions: MS, PDA, DHA, NGA.</li><li>Updated and new control modules.</li></ul>For a comprehensive function overview, please refer to <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/news/140846/clarity-10-released">the full details on our website</a>. Also, check our <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/product/upgrade">Upgrade policy</a>.Upgrade now and experience Clarity 10.0!<blockquote><a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/product/clarity-demo">GET FREE DEMO</a><ul><li>Check the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1&amp;version=10.0">What is new</a>.</li><li>Read the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/downloads/140899/view">Clarity (Lite) 10 vs. 9.0</a> guide.</li></ul></blockquote>&nbsp;Thank you for your trust in our products,Your DataApex team<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Clarity_10_released_91e9897b7d.jpg" alt="Dataapex: Clarity 10 chromatography software released" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Clarity_10_released_91e9897b7d.jpg 227w," sizes="100vw" width="800" height="550"><h3><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">DataApex Clarity Chromatography Software</a></h3>Clarity is a chromatography data software for data acquisition, processing, and instrument control in regulated environment. Clarity is a flexible solution for devices from different vendors, supporting different applications.Optional control modules provide integrated control of selected instruments such as GCs, LCs, pumps, detectors, autosamplers, etc. Extensions provide functions for specific separation techniques or calculations such as PDA, GPC analysis, Mass spectrometry, or SST. Clarity can run in English, French, German, Russian, Spanish, and Chinese language. Support technicians and developers are close to their users and ready to provide extensive Support from DataApex.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Chromatograficky_Software_3_4adabdd9a6/DataApex-Clarity-Chromatograficky-Software-3.png" alt="DataApex: Clarity Chromatography Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_Chromatograficky_Software_3_4adabdd9a6/thumbnail_DataApex-Clarity-Chromatograficky-Software-3.png 230w," sizes="100vw" width="1862" height="1265">

Clarity 10 chromatography software released

<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1001">The PDA Extension</a> is an optional Extension that is used for processing data that has been acquired from selected photo diode array detectors. Spectral data, together with chromatograms, add a third dimension to analytical data analysis. This extension expands the capability of Clarity by providing interactive spectral analysis, peak purity analysis and compound identification that is based on a spectral library search. PDA data can be displayed in a set of optional views including 3D rendering.All data is saved in a single file; a chromatogram at any wavelength or even only a spectrum can simply be recalled for review after an analysis. UV-VIS spectra, acquired using a PDA detector, may be interactively selected from a chromatogram signal for visual inspection and comparison. The spectra may also be used for peak purity determinations, wavelength optimization and component identification through spectral libraries.<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=58&amp;search=pda+extension">The PDA Extension</a> is an optional, fully integrated addition to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.&nbsp;</li><li>It can be ordered as a part of new software or as an extension to existing software.</li><li>The LC-PDA mode is selectable for any LC Instrument within a station.&nbsp;</li><li>The PDA Extension is also compatible with Clarity Offline software.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_PDA_Extension_776dbd48dd.jpg" alt="DataApex - PDA Extension" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_PDA_Extension_776dbd48dd.jpg 245w," sizes="100vw" width="1206" height="534"><h4>Features</h4><ul><li>PDA Data: All data is saved in a single file; a chromatogram at any wavelength or a spectrum only can be simply recalled after an analysis for review. UV-VIS spectra, acquired using a PDA detector, may be interactively selected from a chromatogram signal for visual inspection and comparison. The spectra may also be used for peak purity determinations, wavelength optimization and component identification through spectral libraries.</li><li>PDA View: The PDA view is customizable; up to four views can be displayed at one time (possible views: Isoplot, Chromatogram, Spectra, 3D Display, Peak Purity, Peak Purity Spectra, Library Spectra Search and Spectral Library). The user can easily extract chromatographic signals from PDA data to determine the optimal detection wavelength for each peak.</li><li>PDA Method: The Clarity PDA method includes an option for Spectral Library Search and Peak Purity analysis.</li><li>Peak Purity: This analysis helps to discover hidden impurities. Purity is calculated from 5 or all spectra within the peak. The similarity curve is displayed in the PDA window. Peak Purity analysis can be optimized by setting custom preferences relating to the purity threshold, wavelength restriction and absorbance threshold.</li><li>Spectral Library: The Clarity software compares the peak spectra with the spectra of an unlimited number of spectral libraries. Spectra stored in a Spectral Library include retention times and analysis parameters. The Spectral Library Search can perform automatic identification of integrated/calibrated components (peaks). The library search may be constrained by the RT Window and by Wavelength Range. A Background Correction option is also available.</li><li>Import/Export Data: Spectral data can be imported/exported in ASCII text formats to or from the Clarity software.</li><li>Reports: Users can easily include PDA options such as data 3D Display, Isoplot, Spectra, Peak Purity and Library Search results in reports using the intuitive Report Setup dialog.</li><li>Instrument Control: Control modules (p/n A24) for Agilent, Shimadzu, Knauer and Duratec DADs are available (as of May 2007). Additional control modules for other PDA detectors can be developed upon request.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_The_PDA_Window_displays_four_views_organized_in_splitters_8c614fb6f0.jpg" alt="DataApex: The PDA Window displays four views organized in splitters. Currently displayed in the example are: Isoplot, Chromatogram, Spectral, 3D Display The user can additionaly select also Peak Purity, Peak Purity Spectra, Library Spectra Search and Spectral Library." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_The_PDA_Window_displays_four_views_organized_in_splitters_8c614fb6f0.jpg 191w," sizes="100vw" width="1098" height="896"><h4>Specification</h4><ul><li>Part No.: A29</li><li>Related Products:&nbsp;<ul><li>Clarity (p/n C50) - required</li><li>Clarity offline (p/n C59)</li><li>LC Control (p/n A24) - see the list of available PDA detectors at www.dataapex.com</li></ul></li></ul>

Clarity software - PDA (Photodiode Array) Extension

In a laboratory environment, water quality plays a crucial role in conducting analytical methods, preparing chemical solutions, and ensuring the accuracy and reproducibility of experiments. Even minimal contamination can negatively impact analytical results, making it essential to ensure an appropriate level of water purity.&nbsp;Modern laboratory applications require different levels of water purification depending on the specific process requirements, and the choice of a suitable system depends on several factors, including the quality of the feed water, the required purity, the technological solution, and operational costs. This article focuses on the key aspects to consider when selecting a laboratory water purification system.<h4>Required Water Quality&nbsp;</h4>When selecting a water purification system, it is essential to determine the required water quality according to its intended use:&nbsp;<ul><li>Type I (ultrapure water) – suitable for sensitive analytical methods such as HPLC, ICP-MS, or PCR.&nbsp;</li><li>Type II (pure water) – used for buffer preparation, culture media, or clinical applications.&nbsp;</li><li>Type III (technical water) – suitable for washing laboratory glassware, autoclaves, or other technical applications.&nbsp;</li></ul><blockquote><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/download/Watrex_Water_2024.pdf?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">View the Watrex Praha Water System Catalog.&nbsp;</a></blockquote><h4>Feed Water Quality&nbsp;</h4>Before selecting a system, it is advisable to analyze the feed water, which may come from the municipal water supply or pre-treated sources, to allow the supplier to recommend the most suitable system for your needs. Parameters such as ion content, organic substances, and microbial contamination can influence the suitability of a specific system.&nbsp;<h4>Purification Technologies&nbsp;</h4>Modern water purification systems combine several technologies to achieve the required purity:&nbsp;<ul><li>Filtration – mechanical filtration is the first step in the purification process, removing solid particles such as sediments, rust, and other mechanical impurities. Filters with different pore sizes (e.g., 5 μm or 0.2 μm) are used depending on the required water purity.&nbsp;</li><li>Activated Carbon – this step removes chlorine, organic contaminants, and certain volatile organic compounds (VOCs). Activated carbon absorbs chemicals that could affect analytical methods or cause unwanted reactions.&nbsp;</li><li>Reverse Osmosis (RO) – one of the most effective methods for removing dissolved salts, heavy metals, bacteria, and most organic impurities. This technology uses a semi-permeable membrane that allows water to pass through while retaining most contaminants, significantly reducing water conductivity.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_10_obr_2_f34963c57b.jpg" alt="Watrex img.2: Reverse osmosis filter connection scheme. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_10_obr_2_f34963c57b.jpg 245w," sizes="100vw" width="1600" height="947"><ul><li>Deionization (DI) – this method uses ion exchange resins to replace cations (e.g., Ca²⁺, Mg²⁺, Na⁺) and anions (e.g., Cl⁻, SO₄²⁻) with hydrogen (H⁺) and hydroxide (OH⁻) ions, resulting in nearly ion-free water. This technology is essential for producing ultrapure water.&nbsp;</li><li>Ultraviolet (UV) Radiation – used for water disinfection and organic matter breakdown. UV radiation at a wavelength of 185 nm effectively reduces total organic carbon (TOC) content, while UV at 254 nm is used for bacterial and viral inactivation.&nbsp;</li><li>Ultrafiltration (UF) – this technology utilizes membranes with pore sizes smaller than 0.01 μm, effectively removing bacteria, endotoxins, pyrogens, and other biological contaminants. It is essential in applications requiring the elimination of biological contamination, such as buffer preparation for cell cultures.&nbsp;</li><li>Electrodeionization (EDI) – an advanced technology combining ion exchange resins with an electrochemical regenerator, enabling continuous operation without chemical regeneration. The result is water with exceptionally low conductivity and minimal contamination.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_10_1_e24a944b21.jpg" alt="Watrex img.3: Types of purification technologies. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_10_1_e24a944b21.jpg 245w," sizes="100vw" width="1600" height="755">The choice of the right combination of these technologies depends on the required water purity and its laboratory application.&nbsp;<h4>Capacity and Flow Rate&nbsp;</h4>The system selection should match the laboratory's daily water consumption. For example, small laboratories may require 10–50 L/day, while large research facilities may need hundreds of liters per day. The system's flow rate should be sufficient for efficient operation and rapid container filling.&nbsp;<h4>Maintenance, Service, and Supplier Selection&nbsp;</h4>When selecting a system, it is important to consider operating costs, including filter replacement, resin exchange, and other components. Regular maintenance is essential for maintaining optimal functionality and water quality.&nbsp;In addition to technical parameters, the availability of spare parts and manufacturer service support should also be considered. Reputable suppliers typically offer warranty and post-warranty service, which is crucial for the long-term reliability of the system. Thanks to its own service team, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Watrex Praha</a> provides all needs from maintenance and service to the availability of all necessary spare parts and filters for its <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/water-purification/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">own water systems</a> or HealForce brand water systems.<h4>Conclusion&nbsp;</h4>The selection of a laboratory water purification system should be carefully considered based on the specific requirements of the workplace. Key factors include the required water purity, feed water parameters, purification technologies, capacity, operating costs, and service availability. Investing in the right system ensures optimal conditions for laboratory experiments and the protection of sensitive analytical methods. At Watrex Praha, we are fully available to assist you in selecting the best solution for your needs! Contact us at watrex@watrex.com or visit our website at <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">www.watrex.com.</a>

Selection of a Water Purification System for the Laboratory: Key Factors and Recommendations

Clarity 10.1 expands calibration capabilities to help you work more confidently and efficiently. From clearer guidance and better warnings to flexible internal standard evaluation, this update streamlines calibration development, improves traceability, and supports compliance where it matters most.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_10_1_397aba9bab.jpg" alt="DataApex: Clarity 10.1 released" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Clarity_10_1_397aba9bab.jpg 143w," sizes="100vw" width="849" height="925"><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/P063_Clarity_10_1_New_Features_283323fcd2.pdf">Clarity 10.1 highlights</a></h4><ul><li>ISTD from a different signal</li><li>Determination Coefficient (R2)</li><li>Enhanced calibration warnings and blank handling</li><li>Electronic Signature improvements</li><li>New and updated control modules</li></ul><h5>Internal standard from another signal</h5>Clarity 10.1 introduces support for evaluating results using an internal standard from a different signal within the same chromatogram. This is especially useful for workflows like ICP-MS, where some signals may lack an ISTD peak.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Internal_standard_from_another_signal_2f2ac068f8.jpg" alt="DataApex: Internal standard from another signal" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Internal_standard_from_another_signal_2f2ac068f8.jpg 228w," sizes="100vw" width="380" height="260"><h5>Determination Coefficient (R²)</h5>Clarity now displays the Determination Coefficient (R²) alongside the Correlation Coefficient in each compound's calibration curve tab.Determination Coefficient (R²) gives you a more accurate view of how well your calibration model fits the data - especially valuable during method development and validation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Determination_Coefficient_57673d0ac3.jpg" alt="DataApex: Determination Coefficient" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Determination_Coefficient_57673d0ac3.jpg 228w," sizes="100vw" width="380" height="260"><h5>Enhanced calibration warnings and blank handling</h5>Clarity 10.1 helps you catch issues earlier and set up calibration with greater confidence. Warnings are now more visible and more specific - appearing above both the Results Table and the Calibration Summary Table. When multiple issues occur, an ellipsis appears with full details available on hover.Blank handling has also been clarified: blanks are included only when Ignore Origin is selected. If the origin is used, blanks are excluded, and Clarity now clearly warns and visually shows when a blank is ignored.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Enhanced_calibration_warnings_and_blank_handling_e9fe7fa31e.jpg" alt="Dataapex: Enhanced calibration warnings and blank handling" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Enhanced_calibration_warnings_and_blank_handling_e9fe7fa31e.jpg 228w," sizes="100vw" width="380" height="260"><h5>Signature improvements</h5>Clarity 10.1 improves how signatures are handled, making it easier to understand what is and isn't signed. Chromatograms opened with a linked calibration are always marked Not Signed. Once signed, the mode switches to Stored Calibration, so you always see the final, signed result. Even historical versions now reliably show their signature state and details.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Dataapex_Signature_improvements_a2bebe7875.jpg" alt="Dataapex: Signature improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Dataapex_Signature_improvements_a2bebe7875.jpg 228w," sizes="100vw" width="380" height="260"><blockquote>Explore the complete list of all changes, fixes, and additions in version 10.1.<a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">See the complete changelog</a></blockquote>

Clarity Chromatography Software 10.1 released!

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Clarity Chromatography Software 10.1 released!

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Moving from R&D and Limited QC on a UV-Vis-NIR instrument to Large Scale QC Using an Autosampler

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