We introduce multicolumn online 2D-LC approach that simplifies column screening and method development dramatically enabling us to combine multiple columns in both dimensions.

Comprehensive two-dimensional (2D) chromatography method combining LC, SFC, and HRMS with fragmentation (LC×SFC-HRMS/MS) was developed for structural characterisation of lignin.

The technique for automated comparative analysis of chromatograms across different samples as well as across different detectors using the same sample is demonstrated.

2D-LC/MS in a regulated biopharma environment: Challenges & applications

LC-GC×GC-TOF MS/FID is a powerful multidimensional analytical method for automated and reliable characterization of complex samples. Overview of the technique and application to mineral oil analysis in food.

In this study, the enantioselective separation of a wide range of medicinal substances (log p: 0.9-4.1) is evaluated using a combination of TRLC and Chiral-RPLC.

This study investigates possible configurations for the modulation of HILIC and RPLC using ASM in a selective comprehensive 2D-LC set-up (sHILIC x RPLC).

This presentation will discuss the current challenges to scale this automated method development technology to heart-cut and comprehensive 2D-LC separations.

New improvements and free GCMS training resources, including videos and associated handouts, will be described. EI/tandem libraries utilized include NIST, Wiley, MoNA, and user-created ones.