Metabolomics researchers are often forced to choose between two powerful but different approaches. <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/metabolomics-lipidomics/metabolomics/untargeted-metabolomics.html">Untargeted metabolomics</a> is great for discovery: it surveys as many metabolites as possible to reveal biology you didn’t know to look for. <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/metabolomics-lipidomics/metabolomics/targeted-metabolomics.html">Targeted metabolomics</a> is built for precision: it measures a defined list of metabolites with high sensitivity and quantitative confidence. The challenge is that running both typically costs time, sample, and can introduce variability between workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_One_Injection_Two_Answers_Introducing_Squad_Metabolomics_on_Orbitrap_Mass_Spectrometers_0f7ca7d1c8.jpg" alt="Thermo Fisher Scientific: One Injection, Two Answers: Introducing Squad Metabolomics on Orbitrap Mass Spectrometers" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_One_Injection_Two_Answers_Introducing_Squad_Metabolomics_on_Orbitrap_Mass_Spectrometers_0f7ca7d1c8.jpg 245w," sizes="100vw" width="1024" height="512">In order to deal with this the researchers at Thermo Fisher Scientific have developed <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/metabolomics-lipidomics/simultaneous-quantitation-discovery-squad.html">Simultaneous Quantitation and Discovery (SQUAD)</a> analysis as shown in this technical note <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004327_ov_squad_metabolomics_orbitrap_astral_tn004327_na_en_290c75edd9.pdf">Simultaneous Quantitation and Discovery Analysis: Combining Targeted and Untargeted Metabolomics on Orbitrap Mass Spectrometers</a>, a hybrid strategy that combines targeted quantitation and untargeted discovery in a single experiment. In practice, SQUAD lets you quantify a predefined metabolite panel using calibration curves built from authentic standards and isotopically labeled internal standards, while still capturing rich full-scan data that supports retrospective mining and identification of unknowns.A key theme of the technical note is that results are only as strong as the experiment design and quality controls. The workflow emphasizes calibration strategy, careful extraction choices (aligned to metabolite classes), and the smart use of blanks, pooled QCs, and system suitability tests to monitor drift, contamination, and reproducibility across a batch.SQUAD is also designed to scale across different Thermo Scientific Orbitrap mass spectrometers. The technical note outlines how the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1491">Thermo Scientific Orbitrap Exploris mass spectrometers</a> can use MS1-based SQUAD with polarity switching and optionally add tSIM/tMS2 for targeted sensitivity, while the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=Tribrid">Thermo Scientific Orbitrap Tribrid mass spectrometers</a> can pair high-resolution MS1 discovery with rapid ion-trap tMS2 quantitation, boosted by tools like <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1763">Thermo Scientific AcquireX Intelligent Data Acquisition</a> and Real-Time Library Search (RTLS).Finally, the data story comes together in software: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77">Thermo Scientific TraceFinder Software</a> for targeted quantitation and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer Software </a>for untargeted discovery, annotation, and statistics, turning one injection into both confident numbers and new biological leads.<blockquote><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004327_ov_squad_metabolomics_orbitrap_astral_tn004327_na_en_290c75edd9.pdf">Download the SQUAD technical note</a></div></blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#SQUAD #Metabolomics #Lipidomics

One Injection, Two Answers: Introducing Squad Metabolomics on Orbitrap Mass Spectrometers

<h3><a target="_blank" rel="noopener noreferrer" href="https://www.isc2026.org/">ISC 2026</a></h3>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/314">International Symposium on Chromatography (ISC)</a> represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/644">35th International Symposium on Chromatography</a></h4>📆6. - 10. September 2026📍Prague, Czech Republic<h3>Registration</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://www.czech-in.org/cmPortalV15/Account/Login?ReturnUrl=%2FcmPortalV15%2FPortal%2FISC26%2Fnormal">Register HERE for the ISC 2026</a>Late Registration until 25 August 2026!From 26 August 2026 only Onsite Registration.</blockquote><h4>Publishing Workshop</h4><ul><li>Wednesday, 9 September 2026&nbsp;</li><li>12:30–14:00&nbsp;</li></ul>This interactive workshop will provide practical guidance on scientific publishing in the field of separation science. Participants will have the opportunity to hear directly from journal editors about key aspects of the publication process and how to improve their chances of successful publication.Topics will include manuscript preparation, peer review, editorial decision-making, open access publishing, and the responsible use of artificial intelligence in scientific publishing. Editors will share insights into what they look for in submissions, common reasons for rejection, and strategies for preparing strong manuscripts. The workshop will also cover selected Wiley-specific topics, including the publisher’s submission platform and current guidelines on the use of AI in scientific publications.The session will include opportunities for questions and discussion, allowing participants to interact directly with editors and gain valuable advice for their future publishing activities.<h5>Attending Editors-in-Chief:</h5><ul><li>Michael Lämmerhofer — Journal of Separation Science and Separation Science Plus&nbsp;</li><li>Sebastiaan Eeltink — Analytical Science Advances&nbsp;</li><li>Mario Thevis — Drug Testing &amp; Analysis&nbsp;</li></ul>

Publishing Workshop at ISC 2026

We are excited to announce that the 18th MDCW will take place from January 25-27, 2027, in Liège, Belgium. This in-person event will feature:<ul><li>Keynote presentations from leading researchers</li><li>Flash talks and poster sessions highlighting the latest innovations</li><li>Focus group discussions addressing current challenges and future developments</li><li>💸FREE registration ensures that this event remains accessible to all researchers, students, and industry professionals interested in multidimensional chromatography. ​​We hope to see you there!</li></ul><h3>Registration</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://app.oxfordabstracts.com/auth?redirect=https%3A%2F%2Fapp.oxfordabstracts.com%2Fregister%2Fevent%2F77272%3Fpreview%3Dfalse">Register HERE</a>Registration deadline: December 1, 2026</blockquote><h3>Abstract Submission</h3>Abstracts for oral presentations must be submitted by September 15, 2026, to be considered.​Poster abstracts submitted by September 15, 2026, will be included in the preliminary program. Additional last-minute poster submissions will be accepted until November 30, 2026, for inclusion in the final program.<blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://app.oxfordabstracts.com/auth?redirect=/stages/81780/submitter">Submit your abstract HERE</a>&nbsp;Deadline for abstract submission: September 15, 2026</blockquote><h4>Presentation guidelines</h4><h5>Poster Presentations</h5><ul><li>Presenters must print and bring their own posters—onsite printing services and mailed submissions are not available.</li><li>Posters should be in landscape format: 36" tall × 48" wide.</li><li>Set up your poster during registration on the day of your assigned session and remove it at the end of the same day.</li><li>Poster presenters must be present at their posters during the designated poster session.</li><li>Posters will also be available for viewing during coffee and lunch breaks.</li><li>Presenters may optionally stand by their posters during these times for further discussions.</li></ul><h5>Oral Presentations</h5><ul><li>Keynote presentations: 30 minutes (25–28 min + Q&amp;A)</li><li>Full oral presentations: 20 minutes (18 min + 2 min Q&amp;A)</li><li>Flash presentations: 10 minutes (8 min + 2 min Q&amp;A)</li></ul>Presentation Format:<ul><li>Submit your slides as a PowerPoint (PPT) file (.ppt or .pptx).</li><li>Name your file with program code and surname, e.g. "O-28 Smith.pptx".</li><li>For optimal display, use 16:9 slide format (4:3 is also supported).</li></ul>Technical &amp; Session Logistics:<ul><li>A headset or portable microphone will be available.</li><li>A laser pointer with a slide change button will be provided.</li><li>Ensure your presentation allows time for questions within your allocated time.</li><li>Before your session, check in and introduce yourself to the conference or session chairs.</li></ul><h3>Program</h3><h4>Keynote Speakers</h4><h5>Finding Clarity in Challenging Samples: Multiple Heart-Cutting 2D-LC and the Technologies That Enable It &nbsp; &nbsp;</h5><ul><li>Stephan Buckenmaier (Principal Scientist R&amp;D, Agilent Technologies)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/MDCW_Stephan_Buckenmaier_c84239ec30.jpg" alt="MDCW: Stephan Buckenmaier" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_MDCW_Stephan_Buckenmaier_c84239ec30.jpg 151w," sizes="100vw" width="284" height="294">Stephan Buckenmaier is a Principal Scientist in R&amp;D at Agilent Technologies, specializing in advanced separation science and the development of two-dimensional liquid chromatography (2D-LC) solutions. Since joining Agilent in 2006, he has held a variety of scientific and technical roles, with a particular focus on innovative chromatographic technologies. He earned his Ph.D. from the University of the West of England (Bristol, UK) and conducted postdoctoral research at the University of Tübingen. Stephan has authored numerous scientific publications, contributed a book chapter, and is listed as an inventor on numerous patents in the field of separation science. He regularly presents his work at international scientific conferences.<h4>Short Course</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/18th_MDCW_short_course_41ecd3c6cf.jpg" alt="18th MDCW: Short course" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_18th_MDCW_short_course_41ecd3c6cf.jpg 245w," sizes="100vw" width="1100" height="619">There will be 20 spaces available in a short course related to multidimensional chromatography. If you want to register for the short course, indicate so during your registration. We will send a separate link to pay for the short course to reserve your seat ($50 USD).&nbsp;<h4>A Beginner’s Guide to Multidimensional Chromatography short course</h4><ul><li>Instructors: Pierre-Hugues Stefanuto, Katelynn Perrault Uptmor, Dwight Stoll, Petr Vozka</li></ul><h5>Course Description&nbsp;</h5>So...you want to do multidimensional chromatography? If you are getting started in the field using either liquid or gas chromatography, you’re in the right place. This course will cover a brief history and introduction to both multidimensional gas chromatography and multidimensional liquid chromatography. Discussion will also include basic system components and data handling strategies to apply after acquiring your data. A hands-on portion of the course will also include familiarization with common system components and consumables for both GC and LC, as well as the opportunity to use a commercial GC×GC instrument.<h5>Target Participants&nbsp;</h5>This course is intended to be an initial touchpoint for those who are looking to explore multidimensional chromatography as a new option/capability, or who might be using it for the first time. The course is intended for new users to obtain a background on the techniques as a primer to the workshop. &nbsp;Advanced topics will not be discussed.<h5>Course Logistics&nbsp;</h5><ul><li>Morning Session (8:30 AM – 12:00 PM)</li><li>Afternoon Session (1:00 PM – 4:30 PM)</li></ul>Lunch and coffee will be provided.<h5>Cost&nbsp;</h5>A $50 fee is required to reserve a spot in the course. 20 seats are available. An expression of interest form will be available to indicate interest in a seat within the course. On November 10th, participants will be informed about seat availability and the payment process will be communicated.<h4>Outline (Total Time ~ 4 hrs)&nbsp;</h4><h5>Part I (1 hr) – History, Terminology, Fundamental Concepts&nbsp;</h5><ul><li>Brief history of the development of multidimensional separations&nbsp;</li><li>Terminology&nbsp;</li><li>Fundamental Concepts&nbsp;<ul><li>In what ways are multidimensional separations useful for analytical chemistry / measurement science?&nbsp;</li><li>First and second separations should be orthogonal / complementary&nbsp;</li><li>Fast second dimension separations are important to avoid undersampling&nbsp;</li><li>Structured chromatograms can provide chemical insight and facilitate data interpretation&nbsp;</li><li>Highlights of an example data processing workflow – from raw data to actionable information &nbsp;</li></ul></li><li>Break (30 min)&nbsp;</li></ul><h5>Part II (1.5 hrs) Introduction to Multidimensional Gas Chromatography&nbsp;</h5><ul><li>Method basics&nbsp;<ul><li>Column selection&nbsp;</li><li>Method parameters&nbsp;</li><li>Carrier gas choices&nbsp;</li><li>Detector considerations&nbsp;</li></ul></li><li>Modulator selection with a focus on current market options&nbsp;<ul><li>Thermal modulators&nbsp;</li><li>Flow modulators&nbsp;</li><li>Other commercial options&nbsp;</li></ul></li><li>Method development made simple&nbsp;</li><li>Data preprocessing and initial handling&nbsp;</li><li>Lunch (1 hr)&nbsp;</li></ul><h5>Part III (1 hr) Introduction to Multidimensional Liquid Chromatography&nbsp;</h5><ul><li>Modes of multidimensional separation commonly used with LC&nbsp;<ul><li>Heartcut&nbsp;</li><li>Multiple heartcut / selective comprehensive&nbsp;</li><li>Fully comprehensive&nbsp;</li></ul></li><li>Interfaces used for multidimensional LC (i.e., the hardware that does the transfer from first to second dimension)&nbsp;</li><li>Common use cases of multidimensional LC illustrated using examples from industry and academic research&nbsp;<ul><li>Evaluating peak purity&nbsp;</li><li>Unknown identification via multidimensional and MS or NMR detection&nbsp;</li><li>Multi-stage separation with analyte transformation between stages&nbsp;</li><li>Multi-attribute methods&nbsp;</li><li>Making complete analysis of moderately complex samples attainable in a reasonable analysis time&nbsp;</li><li>Digging deeper – comprehensive separations for comprehensive analysis&nbsp; Introduction to method development strategies&nbsp;</li></ul></li></ul><h5>Part IV (2 hrs) Laboratory Instrument Familiarization</h5><h5>Part V (30 min) Wrap-up and Q&amp;A &nbsp;</h5><h4>Venue</h4><ul><li>University of Liege</li><li>Pl. du Vingt Août 7</li><li>4000 Liège</li><li>​Belgium</li><li>​Main Room: Salle Academique</li></ul>

18th MDCW - Registration and abstract submission opens

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The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Agilent PLgel 1000Å, 7.5 x 300 mm, 5 µm – GPC/SEC Column (Tested)

Agilent PLgel 1000Å GPC/SEC column enables reliable separation of non-polar polymers (200 Da–60 kDa) using organic solvents like THF, toluene, or chloroform.

Agilent GRAM, 100Å, 8 x 300 mm, 10 µm. GPC/SEC column, AMA0830101E2 (Tested)

The Agilent GPC/SEC column for medium polarity organic eluents e.g., DMF, DMAc, DMSO, NMP to analyze polar macromolecules resolving range 100 Da to 60 kDa.

Agilent GPC/SEC Column PL aquagel-OH MIXED-H 8um 300 x 7.5mm (Tested)

Agilent PL aquagel-OH MIXED-H (PL1149-6800, 7.5 × 300 mm, 8 µm) aqueous GPC/SEC column separates polymers and polysaccharides across 500 Da–10 MDa with high resolution and reproducibility.

Agilent AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) PL1580-5301 (Tested)

AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) delivers high-resolution SEC for proteins, mAbs, and aggregates, with a wide MW range (5–1,250 kDa).

Agilent PL aquagel-OH MIXED-H (PL1149-6800) GPC/SEC column provides linear aqueous separation of polymers, starches, and polysaccharides in the range 500 Da–10 MDa.

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