Quantifying steroids in biological matrices has always presented analytical challenges. Complex structures, isobaric interferences, and matrix effects often limit accuracy and throughput — demanding a new approach beyond incremental improvements.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_6e03ce7f59.jpg 245w," sizes="100vw" width="1024" height="512">The<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149"> Thermo Scientific Stellar MS</a> redefines how analysts approach steroid quantification, enabling rapid method optimization through advanced scanning flexibility, complementary fragmentation mechanisms, and enhanced selectivity.Rather than focusing on a single metric, this application note demonstrates how researchers can quickly develop more effective methods by leveraging the full capability of the Stellar MS to characterize compounds comprehensively in a single acquisition.<h3>A new paradigm for analytical quantitation</h3>Traditional LC-MS approaches often rely on incremental improvements in sensitivity or speed. Stellar MS instead provides a new analytical framework, designed to optimize every parameter of quantitative method creation.Key attributes include:<ol><li>PRM vs. SRM/MRM — improving instrument efficiency Parallel Reaction Monitoring (PRM) on Stellar MS provides comprehensive ion information for each target steroid, improving quantitation accuracy and efficiency over traditional MRM methods.</li><li>Complementary dissociation mechanisms — HCD and CID Stellar’s unique ability to combine beam-type (HCD) and resonant (CID) dissociation ensures full coverage of all 17 targeted steroids. This dual-mode approach effectively overcomes neutral water loss product ions — one of the key challenges in steroid analysis.</li><li>Next-level selectivity — MS² to MS³ transition The platform’s ability to perform MS³ experiments enhances selectivity and confidence, particularly in complex biological matrices, where interferences are common.</li><li>Product ion accumulation vs. dwell time — improved ion ratios and sensitivity By accumulating product ions rather than relying on fixed dwell times, Stellar MS achieves superior ion ratio precision and reproducibility — even at low spiking levels — without sacrificing throughput.</li></ol>&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg" alt="Thermo Fisher Scientific: Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer: Figure 1. Representative EIC of 17 steroids in human serum using the Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Advancing_Steroid_Quantification_Unlocking_Sensitivity_and_Selectivity_with_the_Thermo_Scientific_Stellar_Mass_Spectrometer_2_21e509451a.jpg 245w," sizes="100vw" width="800" height="412"><h3>From characterization to confident method creation</h3>The power of Stellar MS lies in its comprehensive acquisition strategy. In this application note, a single injection per spiking level generated 125 distinct scan events, combining:<ul><li>HCD and CID MS² experiments</li><li>Targeted MS³ scans</li><li>Positive/negative polarity switching</li></ul>This workflow enables analysts to rapidly evaluate:<ol><li>Which dissociation mechanism produces the most informative spectra</li><li>Whether MS² or MS³ is best suited for each compound</li><li>The optimal product ions for confident post-acquisition quantitation</li></ol>By providing this complete dataset in a single run, Stellar MS dramatically accelerates method optimization and validation, supporting more efficient and reliable quantitation strategies.<h3>Driving method development efficiency</h3>The real strength of the Stellar MS isn’t just its speed or sensitivity — it’s the ability to experiment, compare, and decide in one workflow. Through simultaneous HCD/CID fragmentation, MS² and MS³ switching, and polarity control, users can determine the optimal analytical conditions across complex steroid panels quickly and confidently.This approach helps labs:<ul><li>Reduce development time</li><li>Maximize data quality</li><li>Improve long-term robustness and reproducibility</li></ul><h3>Conclusion</h3>The Stellar MS platform empowers analytical scientists to move beyond incremental performance metrics toward methodological innovation.By integrating comprehensive characterization and advanced scanning control, it enables faster, more confident decisions in quantitative steroid analysis — setting a new benchmark for flexibility and precision in LC-MS.Ask the Experts: On-Demand WebinarLearn more about how Stellar MS enhances steroid profiling and quantitative workflows. Watch the on-demand webinar where Thermo Fisher scientists discuss:<ul><li>Advanced method development strategies</li><li>Insights from the steroid panel dataset</li><li>Practical examples of leveraging Stellar’s flexibility for confident quantitation</li></ul><blockquote>Watch now: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5117">Unlocking the Power of Quantitative Analysis with Stellar MS</a>Read the full application note: <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/32415">Selective and sensitive measurement of 17 steroids in human serum using a Stellar mass spectrometer</a></blockquote><ul><li>Learn more about <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/stellar-mass-spectrometer/stellar-mass-spectrometer-applications.html">Stellar Mass Spectrometer applications</a></li><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#MassSpectrometry #ClinicalResearch #LCMS #StellarMS</li></ul><blockquote><h5>Dane Walker</h5>Dane Walker is Regional Marketing Manager at Thermo Fisher Scientific, focused on LC-MS systems across EMEA. With a PhD in Surface Science and a background in scientific instrumentation, he blends technical expertise with strategic marketing to drive data-led campaigns. Passionate about communicating science and enabling innovation, Dane enjoys powerboating, cycling, stargazing, and talking endlessly about mass spectrometry.</blockquote>

Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer

Visit the LabRulez booth at <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/871">analytica 2026</a> in Munich, 24. - 27. March 2026! You can find us in Hall A1, Booth A1.506. At the booth, you will be able to explore live demonstrations of how the LabRulez portals work and see selected analytical instruments from our pre-owned marketplace section.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg" alt="LabRulez at Analytica 2026: Hall A1 Floor Plan and Booth Location" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_Analytica_2026_Floor_plan_55e1057e03.jpg 245w," sizes="100vw" width="1516" height="881"><h3>Would you like to learn how the LabRulez portals work?</h3>➡︎ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/">LabRulezLCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/">LabRulezGCMS</a> ➡︎ <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/">LabRulezICPMS</a><ul><li>A unique online solution for your marketing</li><li>A targeted audience from the analytical chemistry community</li><li>Modern technologies and a unique concept</li><li>Design optimized for most smart devices – PC, mobile, and tablet</li><li>Advanced statistical tools fully available to users</li></ul><h3>Services Offered</h3><h4>News + Company Updates</h4><ul><li>Publish news related to your instrumentation, applications, services, events, job openings, and more</li><li>News is shared both on our portals and across our social media channels</li></ul><h4>Library – All Documents in One Place</h4><ul><li>Applications, posters, presentations, technical articles, brochures, specifications</li><li>Fast and advanced search capabilities</li><li>Over 32,000 documents currently available in our database</li></ul><h4>Webinars</h4><ul><li>Upcoming and on-demand webinars from the field of analytical chemistry</li><li>More than 5,500 webinars covering chromatography, mass spectrometry, and spectroscopy</li></ul><h4>Careers</h4><ul><li>Targeted job listings in analytical chemistry</li><li>Clear filtering by specialization or location</li><li>Increased visibility for job postings</li></ul><h4>Companies</h4><ul><li>Company profile with key information and contacts</li><li>Integration of all your activities on our portal: News, Library documents, Webinars, Upcoming Events, Careers, and mentions by partners or customers</li><li>Connection to your Products listed on LabRulez portals</li></ul><h4>Products and Services</h4><ul><li>Comprehensive overview of instrumental techniques and services</li><li>Targeting the analytical chemistry community and customers in the growing online environment</li></ul><h4>Marketplace</h4><ul><li>Buying and selling pre-owned analytical equipment</li><li>We purchase analytical instrumentation of all brands and ages</li><li>Sell your equipment through LabRulez</li><li>Request and purchase complete systems, modules, or spare parts with warranty and service</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg" alt="LabRulez at analytica 2026" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Lab_Rulez_at_analytica_2026_bazar_7edf4a0872.jpg 88w," sizes="100vw" width="1600" height="2844"><h3>What Will Be on Display at Our Booth?</h3>Come and explore selected spare parts and modules from our marketplace section. These include both new, tested, and pre-owned items.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar">LabRulezLCMS Marketplace</a></h4>Spare parts for LC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=378">Vacuum turbo pumps for LC/MS</a></li></ul>Spare parts for HPLC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=397">Degassers for HPLC/UHPLC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=368&amp;search=valve">Automatic valves for HPLC</a></li><li>HPLC components for Agilent 1100, 1200, and 1260 Infinity II series</li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=377">HPLC lamps</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/bazaar?category=48">HPLC columns</a></li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar">LabRulezGCMS Marketplace</a></h4>Spare parts for GC/MS:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=367&amp;search=turbo">Turbo pumps for GC/MS</a></li></ul>Spare parts for GC:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=366&amp;search=EPC">EPC modules</a></li></ul>Sample injection<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar/432">Autosampler 7693A for GC</a></li></ul>Consumables:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/bazaar?category=7">GC columns </a>and selected accessories</li></ul>

LabRulez at analytica 2026

<h4>The future of chromatography is here – are you ready?</h4>Chromatography continues to evolve at an unprecedented pace, driven by the need for sustainability, analytical performance, and digital innovation. This dedicated analytica 2026 conference session brings together leading experts to explore how next-generation GC and LC technologies, advanced hyphenated techniques, microfluidics, additive manufacturing, and data-driven workflows are reshaping analytical science. From greener HPLC strategies and supercritical fluid chromatography to ion mobility–HRMS and multidimensional separations, the programme provides a forward-looking perspective on how laboratories can adapt to emerging analytical challenges and unlock new scientific insights.<blockquote><h5>Companies participating in the analytica 2026 trade fair:</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1200">Agilent Technologies</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1206">ANALYTIKA</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1197">Bruker</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1201">DataApex</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1195">KNAUER</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7049">LabRulez</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1227">LECO</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1202">Markes International</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1214">Metrohm</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1213">PerkinElmer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1216">Restek</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1196">SCIEX</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1230">SciY</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1215">Shimadzu</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1191">Thermo Fisher Scientific</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/events/1217">Wasson-ECE</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/events/1228">Waters Corporation</a></li></ul></blockquote><h4>Programme Tuesday, 24 March 2026, ICM Saal 5</h4><h5>09:30 - 10:00 EuChemS-DAC Tribute to Prof. Dr. Wolfgang Buchberger presented by Dr. Martin Vogel</h5><ul><li>Dr. Martin Vogel | Germany | University of Münster</li></ul><h4>The Future of Chromatography Is Here – Are You Ready? Towards Sustainable HPLC: Are Narrower Columns the Solution? (GDCh)</h4><h5>10:00 - 10:30 From filament to chromatogram: using additive manufacturing to democratise miniaturisation and promote sustainability in analytical laboratories</h5><ul><li>Dr. Thorsten Teutenberg | Germany | Institut für Umwelt &amp; Energie, Technik &amp; Analytik e.V. (IUTA)</li></ul><h5>10:30 - 11:00 Supercritical fluid chromatography as a sustainable analytical method</h5><ul><li>Prof. Dr. Caroline West | France | University of Orleans</li></ul><h5>11:00 - 11:30 Microfluidic versus conventional LC–HRMS for HIV metabolomics: a comparative performance assessment</h5><ul><li>Frederic Lynen | Belgium</li></ul><h5>11:30 - 12:30 Poster Session sponsored by Shimadzu</h5><h4>The Future of Chromatography Is Here – Are You Ready? GC &amp; HPLC: The Cutting-Edge Advances You Need to Know - Part I (GDCh)</h4><h5>12:30 - 13:00 Next-Gen HPLC: A Column Technology Deep Dive</h5><ul><li>Prof. Dr. Gert Desmet | Belgium | Free University Brussels</li></ul><h5>13:00 - 13:30 GC-Based hyphenated techniques for detailed characterization of complex samples</h5><ul><li>Prof. Dr. Giorgia Purcaro | Belgium | University of Liège</li></ul><h5>13:30 - 14:00 Molecular Encoding as a Tool to Enable Data Science-Assisted Analytical Measurements</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/articles?search=Kevin+Schug">Prof. Kevin Schug</a> | USA | The University of Texas at Arlington</li></ul><h5>14:00 - 14:30 Good Resolutions for Bad Pollutants: Ion Mobility–HRMS to Resolve Complex Environmental Mixtures</h5><ul><li>Prof. Dr. Gauthier Eppe | Belgium</li></ul><h4>The Future of Chromatography Is Here – Are You Ready? GC &amp; HPLC: The Cutting-Edge Advances You Need to Know - Part II (GDCh)</h4><h5>15:00 - 15:30 GC×GC-TOFMS for Medical Volatolomics</h5><ul><li>Prof. Dr. Jean-François Focant | Belgium | University of Liege</li></ul><h5>15:30 - 16:00 Cryogenic band compression as a means to simplify the sample preparation step in 1D and comprehensive 2D GC food analysis</h5><ul><li>Prof. Dr. Peter Q. Tranchida | Italy | University of Messina</li></ul><h5>16:00 - 16:30 Significance of radial dispersion to effective modulation in two-dimensional liquid chromatography and its role in machine-learning based automated method development</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/articles?search=bob+pirok">Bob Pirok</a> | Netherlands | University of Amsterdam</li></ul><h5>16:30 - 17:00 LCxSFC coupling: A robust tool to aid understanding of renewable and circular industrial processes&nbsp;</h5><ul><li>Karine Faure | France | University of Lyon</li></ul>

Analytica 2026: The Future of Chromatography Is Here – Are You Ready?

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Agilent PLgel 1000Å, 7.5 x 300 mm, 5 µm – GPC/SEC Column (Tested)

Agilent PLgel 1000Å GPC/SEC column enables reliable separation of non-polar polymers (200 Da–60 kDa) using organic solvents like THF, toluene, or chloroform.

Agilent GRAM, 100Å, 8 x 300 mm, 10 µm. GPC/SEC column, AMA0830101E2 (Tested)

The Agilent GPC/SEC column for medium polarity organic eluents e.g., DMF, DMAc, DMSO, NMP to analyze polar macromolecules resolving range 100 Da to 60 kDa.

Agilent GPC/SEC Column PL aquagel-OH MIXED-H 8um 300 x 7.5mm (Tested)

Agilent PL aquagel-OH MIXED-H (PL1149-6800, 7.5 × 300 mm, 8 µm) aqueous GPC/SEC column separates polymers and polysaccharides across 500 Da–10 MDa with high resolution and reproducibility.

Agilent AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) PL1580-5301 (Tested)

AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) delivers high-resolution SEC for proteins, mAbs, and aggregates, with a wide MW range (5–1,250 kDa).

Agilent PL aquagel-OH MIXED-H (PL1149-6800) GPC/SEC column provides linear aqueous separation of polymers, starches, and polysaccharides in the range 500 Da–10 MDa.

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LabRulez at analytica 2026

Advancing Steroid Quantification: Unlocking Sensitivity and Selectivity with the Thermo Scientific Stellar Mass Spectrometer

Analytica 2026: The Future of Chromatography Is Here – Are You Ready?