1220 Infinity/Infinity II LC - Site Preparation Checklist

Importance of the Topic

Ensuring optimal site preparation for high-performance liquid chromatography (HPLC) systems is critical for consistent analytical results and extended instrument lifespan. Proper planning of space, environmental conditions, and utilities minimizes installation delays and protects instrumentation from performance issues over time.

Objectives and Study Overview

This summary outlines the key requirements and guidelines defined by Agilent for preparing a laboratory site to install a 1260 Infinity/Infinity II LC system. It covers bench space allocation, environmental controls, power needs, necessary consumables, and special considerations for specific modules and upgrades.

Methodology and Instrumentation

Site preparation recommendations are organized into several categories:

• Bench Space: Minimum clearance of 2.5 cm on each side and 8 cm at the rear for air circulation. Bench must support weights up to 43 kg for module G4294B.
• Environmental Conditions: Stable ambient temperature (0–55 °C) with fluctuations below 1–2 °C/h and humidity below 95% non-condensing.
• Power Supply: Single-phase 100–240 V (±10%), 50–60 Hz outlets, providing up to 240 VA per module and appropriate grounding.
• Operating Supplies: HPLC-grade water (500 mL), vials, and a UV-transparent solvent for initial system checks.
• Modules Covered: G4286B, G4288B/C, G4290B/C, G4294B, and optional ALS cooler specifications.

Main Results and Discussion

The checklist ensures that all hardware components fit designated bench space and operate within manufacturer-specified ranges. Adhering to temperature and humidity guidelines reduces baseline drift and electronic condensation risks. Correct power management prevents electrical overloads and heat build-up. Having required consumables on hand accelerates installation and validation processes.

Benefits and Practical Applications

Following this site preparation checklist:

• Shortens installation time and avoids rescheduling costs.
• Enhances chromatographic stability and reproducibility.
• Reduces maintenance interventions due to environmental stress.
• Supports compliance with quality control and regulatory requirements.

Future Trends and Applications

Advancements likely include greater automation in site verification through IoT sensors, remote monitoring of environmental conditions, integration with lab information management systems (LIMS), and adoption of greener solvents and energy-efficient modules to further minimize the laboratory’s footprint.

Conclusion

Comprehensive site preparation is a foundational step for reliable HPLC operations. By systematically addressing space, environment, power, and consumables, laboratories can ensure seamless installation and maximize the performance and longevity of their Agilent 1260 Infinity LC systems.

References

Agilent Technologies. Site Preparation Checklist for 1260 Infinity/Infinity II LC, Revision 3.0, August 24, 2016.