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A P P L I C AT I O N S N O T E B O O K www.waters.com Introduction Most chromatographers have experienced problems retaining and separating polar compounds using conventional reversed-phase (RP) chromatography. These difficult-to-analyze compounds either…
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VOL 18.1 TheReporter www.sigma-aldrich.com/TheReporter New Paradigm for HPLC Method Development Column: Mobile Phase: Discovery, 5cm x 4.6mm ID, 5µm particles A: O.1% HCO2H (pH 2.5) B: MeCN 0 - 4% in 0.8 min., 4 - 30% in 1.7 min. 3mL/min…
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Fast Gradients for RP-HPLC
2000|Merck|Technical notes
VOL 18.6 TheReporter www.sigma-aldrich.com/TheReporter (continued on page 3) LC issue Conditions for A, B & C differ only in column length, flow and gradient. Panel A. Column: Discovery C18, 15cm x 4.6mm ID, 5µ Cat. No.: 504955 Sample: Gradient RP…
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Troubleshooting Liquid Chromatographic Separations: Avoiding Common Issues through Informed Method Development Practices Dave S. Bell Supelco, Div. of Sigma-Aldrich Bellefonte, PA 16823 USA sigma-aldrich.com/analytical T414096 © 2014 Sigma-Aldrich Co. All rights reserved. Objective •Most of the difficult problems encountered are…
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