New Paradigm for HPLC Method Development
Technical notes | 2000 | MerckInstrumentation
HPLC
IndustriesManufacturerMerck
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Fast Gradients for RP-HPLC
2000|Merck|Technical notes
VOL 18.6 TheReporter www.sigma-aldrich.com/TheReporter (continued on page 3) LC issue Conditions for A, B & C differ only in column length, flow and gradient. Panel A. Column: Discovery C18, 15cm x 4.6mm ID, 5µ Cat. No.: 504955 Sample: Gradient RP…
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Use Discovery to Reduce HPLC Method Development Time
2003|Merck|Technical notes
595 North Harrison Road Bellefonte, PA 16823-0048 USA Telephone 800-247-6628 • 814-359-3441 Fax 800-447-3044 • 814-359-3044 email: supelco@s ial.com sigma-aldrich.com/supelco Bulletin 937 Use Discovery to Reduce HPLC Method Development Time Following the Discovery method development protocol and using the Column…
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Optimizing HPLC Separations: Samples with Widely Differing Polarities
2000|Merck|Technical notes
VOL 18.3 TheReporter www.sigma-aldrich.com/TheReporter Optimizing HPLC Separations: Samples with Widely Differing Polarities When confronted with a sample containing widely varying polarities, the LC chromatographer will typically rely on gradient elution. However, if gradient elution is not an option, isocratic elution…
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HPLC Column Comparison Screening Study for Reversed Phase Columns
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HPLC Column Comparison Screening Study for Reversed Phase Columns Carmen T. Santasania, David S. Bell, and Tracy L. Ascah-Ross Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA T403157 GIB Introduction The selection of the proper HPLC column for any…
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