Fast and Efficient Peptide Mapping of a Monoclonal Antibody (mAb): UHPLC Performance with Superficially Porous Particles | LabRulez LCMS
 

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AdvanceBio PeptideMappingAn HPLC Column Technology forFaster Protein BiocharacterizationsTim RiceBioColumn Technical Specialist1September 10, 2014 What Is Peptide Mapping?•The chemical or enzymatic treatment of a protein to produce peptidefragments•Separation and identification of these fragments in a reproducible manner•For bio-therapeutic proteins and peptides peptide...
Key words
peptide, peptidemapping, mappingmau, mauadvancebio, advancebiodeamidated, deamidatedtryptic, trypticcolumn, columnmin, mintimes, timesmelittin, melittindigest, digestmean, meanigg, igguhplc, uhplcconserved
Agilent-NISTmAbPeptide MappingAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of analysis...
Key words
peptide, peptidemapping, mappingpage, pagecontents, contentsback, backadvancebio, advancebiocdr, cdrpeptides, peptidesagilent, agilentassaymap, assaymapmin, minmap, mapattributes, attributespqas, pqasmsd
Enhancing The Quality Of PeptideMapping Separation For TheAnalysis Of PTMApplication NoteBiotherapeutics and BiosimilarsAuthorIntroductionSuresh Babu C.V.Post-translational modifications (PTMs) on proteins such as monoclonal antibodies(mAbs) are critical quality attributes (CQAs) that define the drug efficacy andsafety. Characterization of PTMs is a difficult...
Key words
peptide, peptidecounts, countsntaylqmnslr, ntaylqmnslradvancebio, advancebiodltmisr, dltmisrmab, mabeeeqynstr, eeeqynstrptms, ptmsdeamidated, deamidatedplus, plusdigest, digestmapping, mappingseparation, separationtryptic, trypticnonoxidized
High Resolution and Rapid PeptideMapping of Monoclonal AntibodyUsing an Agilent 1290 Infinity UHPLCand an Agilent 6550 iFunnel Q-TOFLC/MS SystemApplication NoteAuthorsIntroductionNing TangPeptide mapping is the common analytical technique for characterizing biologictherapeutic proteins. The protein is first digested into smaller peptides enzymaticallyusing...
Key words
peptide, peptidemapping, mappingcounts, countssuperficially, superficiallysequence, sequencejetstream, jetstreamidentified, identifiedacquisition, acquisitionmonoclonal, monoclonalantibody, antibodyefficiency, efficiencyptms, ptmstime, timeagilent, agilentcharge
 

Similar PDF

Toggle
AdvanceBio PeptideMappingAn HPLC Column Technology forFaster Protein BiocharacterizationsTim RiceBioColumn Technical Specialist1September 10, 2014 What Is Peptide Mapping?•The chemical or enzymatic treatment of a protein to produce peptidefragments•Separation and identification of these fragments in a reproducible manner•For bio-therapeutic proteins and peptides peptide...
Key words
peptide, peptidemapping, mappingmau, mauadvancebio, advancebiodeamidated, deamidatedtryptic, trypticcolumn, columnmin, mintimes, timesmelittin, melittindigest, digestmean, meanigg, igguhplc, uhplcconserved
Agilent-NISTmAbPeptide MappingAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of analysis...
Key words
peptide, peptidemapping, mappingpage, pagecontents, contentsback, backadvancebio, advancebiocdr, cdrpeptides, peptidesagilent, agilentassaymap, assaymapmin, minmap, mapattributes, attributespqas, pqasmsd
Enhancing The Quality Of PeptideMapping Separation For TheAnalysis Of PTMApplication NoteBiotherapeutics and BiosimilarsAuthorIntroductionSuresh Babu C.V.Post-translational modifications (PTMs) on proteins such as monoclonal antibodies(mAbs) are critical quality attributes (CQAs) that define the drug efficacy andsafety. Characterization of PTMs is a difficult...
Key words
peptide, peptidecounts, countsntaylqmnslr, ntaylqmnslradvancebio, advancebiodltmisr, dltmisrmab, mabeeeqynstr, eeeqynstrptms, ptmsdeamidated, deamidatedplus, plusdigest, digestmapping, mappingseparation, separationtryptic, trypticnonoxidized
High Resolution and Rapid PeptideMapping of Monoclonal AntibodyUsing an Agilent 1290 Infinity UHPLCand an Agilent 6550 iFunnel Q-TOFLC/MS SystemApplication NoteAuthorsIntroductionNing TangPeptide mapping is the common analytical technique for characterizing biologictherapeutic proteins. The protein is first digested into smaller peptides enzymaticallyusing...
Key words
peptide, peptidemapping, mappingcounts, countssuperficially, superficiallysequence, sequencejetstream, jetstreamidentified, identifiedacquisition, acquisitionmonoclonal, monoclonalantibody, antibodyefficiency, efficiencyptms, ptmstime, timeagilent, agilentcharge
 

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