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In Depth Analysis of Host Cell Proteins from Antibody Preparations using PASEF

 

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Confident LC-MS Identification of Low ppm Host Cell Proteins (HCPs) in Biotherapeutic Monoclonal Antibodies Amy J Claydon1, Phil Widdowson1, Andrew Williamson2, and Min Du3, Thermo Fisher Scientific, 1Runcorn, UK, 2Hemel Hempstead, UK, 3Cambridge, MA, USA Label-free quantification of NISTmAb HCPs…
Key words
hcps, hcpshcp, hcpppm, ppmnistmab, nistmabdynamic, dynamicintrasample, intrasampleidentifications, identificationsclathrin, clathrinhydratase, hydratasetransketolase, transketolaseper, pergranulins, granulinsbisphosphate, bisphosphatemeasured, measureddigest
Biopharma Whitepaper Advanced host cell protein (HCP) analysis with TIMS QTOF MS powers biopharmaceutical development Introduction The recent rapid expansion of the biopharmaceutical field is driving the growth of biotherapeutic analysis and bioprocess development. The percentage of large molecules in…
Key words
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[ APPLICATION NOTE ] A Comprehensive Approach for HCP Identification, Quantification, and Monitoring Based on a Single Dimension (1D) LC Separation Catalin Doneanu, 1 Sarah Lennon, 2 Malcolm Anderson, 2 Ian Reah, 3 Mal Ross, 3 Steven Anderson, 3 Ian…
Key words
biphosphate, biphosphatealdolase, aldolasefructose, fructosehcp, hcpsonar, sonarhcps, hcpstpqiqvysr, tpqiqvysrtvywdrdm, tvywdrdmmse, msenote, notehost, hostaddgrpfpqvik, addgrpfpqvikphpypaltpeqk, phpypaltpeqksqvqasytfk, sqvqasytfktlhqskpvtitvqgpk
PASEF™ on a timsTOF Pro defines new performance standards for shotgun proteomics with dramatic improvements in MS/MS data acquisition rates and sensitivity Trapped ion mobility spectrometry coupled with quadrupole time-of-flight mass spectrometry (timsTOF Pro) offers a unique dimension of characterization…
Key words
tims, timspasef, paseftimstof, timstofprecursors, precursorstunnel, tunnelbruker, brukermobility, mobilityshotgun, shotgundaltonics, daltonicspro, proproteomics, proteomicstrapped, trappedaccumulation, accumulationion, ionparallel
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