Thermo Scientific Vanquish Pumps - PRODUCTION SPECIFICATIONS

Significance of the Topic

High performance liquid chromatography pumps are critical for reproducible separation and quantification in analytical laboratories. The Thermo Scientific Vanquish platform addresses the need for high throughput, sensitivity and system robustness.

Objectives and Overview

This specification outlines the design and performance of the Thermo Scientific Vanquish series of HPLC and UHPLC pumps, covering variants such as Horizon, Flex, Core and Duo. The platform supports binary, quaternary, dual-gradient and isocratic modes to accommodate diverse chromatographic workflows.

Used Instrumentation

• Thermo Scientific Vanquish HPLC and UHPLC pumps including Horizon, Flex, Core and Duo models
• SmartFlow precision pumping technology
• Viper fingertight fittings for simplified setup
• Built-in multichannel solvent degassing modules
• Chromeleon Chromatography Data System with GLP audit trail

Methodology and Instrumentation

The Vanquish pumps utilize parallel or serial dual-piston designs with independent drives and compressibility compensation to maintain precise flow across pressures up to 150 MPa. Settable flow rates range from 0.001 to 10 mL/min in 1 µL increments. Mixer volumes and dwell volumes are optimized via proprietary capillary and static mixer configurations. Wetted parts comprise biocompatible materials such as MP35N, titanium, ceramics, PEEK and UHMWPE.

Main Results and Discussion

• Flow accuracy ±0.1% and precision <0.05% RSD
• Pulsation below 0.4% or 0.2 MPa
• High-pressure gradient proportioning accuracy within ±0.2% full scale
• Multiple solvent lines (up to six) for complex gradient methods
• Dwell volumes from 35 to 679 µL depending on model
• Integrated degassing on up to six channels
• GLP predictive maintenance scheduling and full parameter logging

Benefits and Practical Applications

• High sensitivity and reproducibility facilitate trace-level quantification
• Modular configurations support routine HPLC, ultrafast UHPLC and LC-MS analyses
• Durable components minimize downtime in QA/QC and industrial settings
• Flexible gradient and isocratic operations accelerate method development

Future Trends and Potential Applications

Emerging developments include extending pressure capabilities beyond 150 MPa, microflow and nanoflow integration, expanded biocompatibility, AI-driven maintenance, and multiplexed pumping for higher throughput in proteomics and pharmaceutical screening.

Conclusion

The Thermo Scientific Vanquish pump series delivers unmatched precision, robustness and flexibility for modern chromatographic demands, ensuring confidence in retention time, quantification and system uptime across a wide range of analytical applications.