Biotage VacMaster 10 & 20 Sample Processing Manifold - User Manual

Importance of the Topic

Vacuum‐assisted manifolds are central in modern sample preparation, providing consistent flow rates, high throughput, and safe handling of hazardous or biological samples. The Biotage VacMaster 10 & 20 addresses these needs with robust design, inert materials, and flexible configurations.

Objectives and Overview

This summary reviews the design, specifications, and practical use of the VacMaster manifolds. It highlights key features, operational guidelines, and accessory options for efficient solid-phase extraction (SPE) and related sample processing techniques.

Methodology and Instrumentation

The VacMaster unit applies vacuum (up to –20" Hg) to draw samples or eluents through SPE columns into collection vessels or a waste trap. Key components include:

• Glass tank with reorientable waste outlet fitting for contained disposal
• PTFE lid fitted with vacuum gauge, fine and coarse control valves
• 316 stainless steel or inert PTFE needles and stopcocks
• Adjustable UHMW polyethylene/polypropylene tube racks accommodating 10–27 mm tubes
• Optional accessories: alternative rack plates, PTFE T-valve, combined needle stopcocks, large volume extraction (LVE) kits, vacuum control unit or pump

Main Results and Discussion

The VacMaster design demonstrates:

• Rapid assembly: easy threading of needles and quick rack height adjustment
• Precise vacuum regulation: combined coarse/fine valves and integrated gauge
• High chemical inertness: glass, PTFE, UHMW PE, polypropylene, 316 SS prevent contamination
• Safe operation under biohazard conditions: optional dedicated elution tank and safety screen recommendations
• High throughput capacity: simultaneous processing of up to 10 or 20 samples

Benefits and Practical Applications

Laboratories benefit from reduced processing time, reproducible extraction performance, and compatibility with standard SPE cartridges. The system’s adaptability supports environmental water analysis, pharmaceutical QA/QC, clinical sample cleanup, and hazardous material workflows.

Future Trends and Potential Uses

Emerging developments may include automated vacuum control with digital feedback, expanded cartridge and vial compatibility, in-line sensors for real-time flow monitoring, and integration with laboratory information management systems (LIMS). Scaling the design to higher port counts and remote operation could further enhance sample throughput.

Conclusion

The Biotage VacMaster 10 & 20 offers a user-friendly, reliable vacuum manifold solution for high-throughput SPE. Its modular, inert construction and precise control features make it a versatile asset across analytical chemistry laboratories.

Reference

Biotage VacMaster 10 & 20 Sample Processing Manifold User Manual, Biotage 2020.