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Optimizing Top Down Analysis of Proteins on Orbitrap Fusion Lumos Tribrid Mass Spectrometer

Posters | 2015 | Thermo Fisher Scientific | ASMSInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
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MSUM: NEW on Thermo Scientific Orbitrap Fusion Lumos Tribrid MS
2016|Thermo Fisher Scientific|Presentations
NEW on Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ MS Stephane Houel, Ph.D. BioPharma Vertical Marketing The world leader in serving science NEW On Thermo Scientific Orbitrap Fusion Lumos MS in 2017 ADVANCED PEAK DETERMINATION ALGORITHM RESULTS IN SIGNIFICANT IMPROVEMENT IN…
Key words
uvpd, uvpdapd, apdcoverage, coverageunique, uniqueetd, etdexisting, existingnew, newlumos, lumossystems, systemsoptional, optionalpeptide, peptidetrap, trapproteomics, proteomicsfragmentation, fragmentationdeamidated
Poster Note 64778 Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer Seema Sharma, Stephane Houel, Christopher Mullen, Chad Weisbrod, Romain Huguet, John Syka, Dave Horn, Jonathan Josephs, Jae Schwartz, Vlad Zabrouskov Thermo Fisher Scientific, San…
Key words
abundance, abundancefragmentation, fragmentationantibody, antibodyintact, intactetd, etdrelative, relativemab, mabtop, topdown, downheavy, heavymonoclonal, monoclonaldeglycosylated, deglycosylateduvpd, uvpdpulses, pulsessequence
proteins on a proteomics scale is challenging and requires significant method optimization of front–end separation, instrument parameters and data analysis. In this study, we developed a general RP-LC-MS method for intact/ top-down analysis on an Orbitrap Fusion mass spectrometer using…
Key words
protein, proteinfusion, fusionintact, intacttop, toporbitrap, orbitrapfigure, figuredown, downenolase, enolasefragmentation, fragmentationmixture, mixtureproteins, proteinsyeast, yeasthorse, horseethcd, ethcdwere
Optimization of RP-LC-MS Top-down Protein Analysis on an Orbitrap Fusion Lumos Tribrid MS with the Advanced Peak Determination Algorithm Vlad Zabrouskov1, Luca Fornelli2, Ryan Fellers2, Kristina Srzentic2, Joshua Silveira1, Helene Cardasis1, Graeme C. McAlister1, Christian Thoeing3, Derek Bailey1, Andreas Kuehn3,…
Key words
apd, apdresolution, resolutioncharge, chargeintact, intactdependent, dependentpierce, pierceprotein, proteinisotopically, isotopicallyorbitrap, orbitrapetd, etdhigh, highdata, dataunassigned, unassignedfusion, fusionspd
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