Optimization of LC/MS Intact /Top-Down Protein Analysis on an Orbitrap Fusion Mass Spectrometer
Posters | 2015 | Thermo Fisher Scientific | ASMSInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
IndustriesProteomics
ManufacturerThermo Fisher Scientific
Key wordsprotein, fusion, intact, top, orbitrap, figure, down, enolase, fragmentation, mixture, proteins, yeast, ethcd, horse, were, pmol, microflow, mass, cythochrome, prosightpd, rnasea, proteomics, trypsin, rslcnano, sod, sliding, spectrometer, bsa, hcd, ribosomal, inhibitor, scientific, proswift, respect, using, window, proteome, bovine, carbonic, deconvolution, monolithic, seven, analysis, method, soybean, all, performed, cid, nodes, prosightpdtm
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Optimizing Top Down Analysis of Proteins on Orbitrap Fusion Lumos Tribrid Mass Spectrometer
2015|Thermo Fisher Scientific|Posters
Methods Sample Preparation and Liquid Chromatography Protein standards (Ubiquitin, Carbonic anhydrase and Enolase) were purchased from Sigma Aldrich. Proteins were infused at 1pmol/ul at a flow rate 5ul/min for direct infusion experiments. Recombinant Human TROP2 protein (extracellular domain) was purchased…
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etd, etdlumos, lumosorbitrap, orbitraptop, topdown, downfusion, fusionintact, intactproteins, proteinsfragmentation, fragmentationenolase, enolasecoverage, coveragesequence, sequenceprotein, proteincarbonic, carbonicwere
IMSC: Optimization of crosslinked peptide analysis on an Orbitrap Fusion Lumos mass spectrometer
2016|Thermo Fisher Scientific|Posters
Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…
Key words
crosslinked, crosslinkedbuurbu, buurbucleavable, cleavablecrosslinkers, crosslinkersethcd, ethcdprotein, proteinpeptides, peptidesdsso, dssocrosslinking, crosslinkingidentified, identifiedxlinkx, xlinkxsequestht, sequesthtcid, cidlumos, lumosbsa
Optimization of Crosslinked Peptide Analysis on an Orbitrap Fusion Lumos Mass Spectrometer
2016|Thermo Fisher Scientific|Posters
evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…
Key words
buurbu, buurbucrosslinked, crosslinkedcleavable, cleavablecrosslinkers, crosslinkersdsso, dssoethcd, ethcdsequestht, sequesthtlumos, lumoscrosslinking, crosslinkingfusion, fusiondss, dsscid, cidorbitrap, orbitrapxlinkx, xlinkxpeptide
Optimization of RP-LC-MS Top-down Protein Analysis on an Orbitrap Fusion Lumos Tribrid MS with the Advanced Peak Determination Algorithm
2018|Thermo Fisher Scientific|Posters
Optimization of RP-LC-MS Top-down Protein Analysis on an Orbitrap Fusion Lumos Tribrid MS with the Advanced Peak Determination Algorithm Vlad Zabrouskov1, Luca Fornelli2, Ryan Fellers2, Kristina Srzentic2, Joshua Silveira1, Helene Cardasis1, Graeme C. McAlister1, Christian Thoeing3, Derek Bailey1, Andreas Kuehn3,…
Key words
apd, apdresolution, resolutioncharge, chargeintact, intactdependent, dependentpierce, pierceprotein, proteinisotopically, isotopicallyorbitrap, orbitrapetd, etdhigh, highdata, dataunassigned, unassignedfusion, fusionspd