ASMS: Optimization of Crosslinked Peptide Analysis on an Orbitrap Fusion Lumos Mass Spectrometer
Posters | 2016 | Thermo Fisher ScientificInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
IndustriesProteomics
ManufacturerThermo Fisher Scientific
Key wordsbuurbu, crosslinked, cleavable, crosslinkers, dsso, ethcd, sequestht, lumos, crosslinking, fusion, dss, cid, orbitrap, nce, xlinkx, peptide, isolation, hcd, max, thermo, quadruple, protein, fragment, parameters, bsa, tolerance, scientific, target, etd, value, utrecht, different, peptides, resolution, mass, static, were, identified, search, width, injection, lysates, using, proteome, solublized, fragmentation, scheltema, node, mueller, exactive
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Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…
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crosslinked, crosslinkedbuurbu, buurbucleavable, cleavablecrosslinkers, crosslinkersethcd, ethcdprotein, proteindsso, dssopeptides, peptidescrosslinking, crosslinkingidentified, identifiedxlinkx, xlinkxsequestht, sequesthtcid, cidlumos, lumosbsa
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faims, faimsscx, scxpeptides, peptidescrosslinked, crosslinkeddss, dsscross, crossfractionation, fractionationlinked, linkedmass, massnce, ncemax, maxunmodified, unmodifiedpierce, piercesettings, settingshcd
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2018|Thermo Fisher Scientific|Posters
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aadsbso, aadsbsocrosslinked, crosslinkedbiotinylation, biotinylationultralink, ultralinkstreptavidin, streptavidindsso, dssoneutravidin, neutravidincrosslinks, crosslinksbsa, bsadsbso, dsbsopeptides, peptideshela, helaagarose, agaroseenrichment, enrichmentavidin